AIM： To evaluate the multi-step pretargeting radioimmunoimaging （RII） and radioimmunotherapy （RTT） in nude mice bearing human colon carcinoma with avidin-biotin system labeled with 153^Sm. METHODS： Two- and thre...AIM： To evaluate the multi-step pretargeting radioimmunoimaging （RII） and radioimmunotherapy （RTT） in nude mice bearing human colon carcinoma with avidin-biotin system labeled with 153^Sm. METHODS： Two- and three-step strategies for avidinbiotin system pretargeting techniques were established. In a three-step procedure, human colon carcinoma bearing nude mice were first injected with biotinylated monoclonal antibody （McAb-Bt） followed by cold avidin （Av） 48 h later and then 153^Sm-DB2 24 h thereafter; whereas the twostep procedure consisted of injection of 153^Sm-SA 48 h after pretargeting with biotinylated anti-CEA monoclonal antibody （CEA McAb-Bt）. SPECT imaging and biodistribution were performed at 4, 24, 48, or 72 h after injection of 153^Sm-labeled compounds. Five groups of nude mice subcutaneously grafted with human colon carcinoma were treated 3 d after grafting. One group received the injection with 100 μg CEA McAb-Bt followed by cold avidin （80 μg） after 2 d and 11.1 MBCl I53Sm-DB2 after 1 d. Four control groups were treated respectively with 11.1 MBq 153^Sm- CEA McAb, ii.i MBq 153^Sm-nmIgG, ii.i MBq 153^Sm-DB2, 100 μL normal saline. Toxicity was evaluated by changes of leukocyte count, and the efficacy by variation in tumor volume. Histological analyses of tumors were performed. RESULTS： The three-step procedure allowed faster blood clearance and yielded higher tumor blood ratios （5.76 at 4 h and 12.94 at 24 h） of the 153^Sm-DB2. The tumor was clearly visualized at 4 h in y-imaging after the injection of 153^Sm-DB2, while a significant accumulation of 153^Sm-SA in the tumor was observed only 24 h after the injection and tumor blood ratios at 4 and 24 h were 1.00 and 2.03, respectively, in the two-step procedure. Pretargeting RIT and 153^Sm-CEA McAb had a strong tumor-inhibiting effect.The tumor inhibitory rate was 80.67% and 78.44%, respectively, five weeks after therapy. Histopathological evidence also indicated radioactive damage in tumor tissues as necrosis of tumor cells, while in the other organs such as liver and kidney no radioactive damage was observed. Leukocyte counts showed significant decrease after treatment in groups of 153^Sm-CEA McAb and 153^Sm- nmIgG. CONCLUSION： The two kinds of pretargeting strategies can elevate the target-to-nontarget ratio, decrease the blood background and shorten the imaging time compared to 153^Sm-CEA McAb. Three-step pretargeting RIT is as effident as 153^Sm-CEA McAb, but markedly less toxic. This study provides experimental evidence for the clinical application of pretargeting RII and RIT.展开更多
Objective At present,there is no appropriate system to evaluate the severe complications of liver surgery through the preoperative factors.This study aimed to design and verify a risk assessment system for the predict...Objective At present,there is no appropriate system to evaluate the severe complications of liver surgery through the preoperative factors.This study aimed to design and verify a risk assessment system for the prediction of severe post-operative complications after a hepatectomy based on the preoperative parameters.Methods A retrospective analysis was performed on 1732 patients who had undergone liver surgery.The severity of the complications was graded by Accordion Severity Grading of post-operative complications.The variables were screened by multivariate analysis,and graded scores were assigned to the selected variables.A logistic regression equation was used to form the liver operation risk formula(LORF)for the prediction of severe post-operative complications.The LORF was verified by the receiver operating characteristic(ROC)curve.Results The multivariate correlation analysis revealed the independent influencing factors of the severe post-operative complications of liver surgery were Child-Pugh grade(OR=4.127;P<0.001),medical diseases requiring drug treatment(OR=3.092;P<0.001),the number of liver segments to be removed(OR=2.209;P=0.006),organ invasion(OR=4.538;P=0.024),and pathological type(OR=4.023;P=0.002).The binomial logistic regression model was established to obtain the calculation formula(LORF)of the severe complication risk.The area under the ROC curve(AUC)of the LORF was 0.815.The cut-off value of the expected probability of severe complications was 0.3225(32.25%).Furthermore,in the validation data set,the corresponding AUC of the LORF was 0.829.Conclusion As a novel and simplified assessment system,the LORF could effectively predict the severe post-operative complications of liver surgery through the preoperative factors,and therefore it could be used to evaluate the risk of severe liver surgical complications before surgery.展开更多
BACKGROUND At present,there is no perfect system to evaluate pulmonary complications of liver surgery using perioperative variables.AIM To design and verify a risk assessment system for predicting postoperative pulmon...BACKGROUND At present,there is no perfect system to evaluate pulmonary complications of liver surgery using perioperative variables.AIM To design and verify a risk assessment system for predicting postoperative pulmonary complications(PPCs)after hepatectomy based on perioperative variables.METHODS A retrospective analysis was performed on 1633 patients who underwent liver surgery.The variables were screened using univariate and multivariate analyses,and graded scores were assigned to the selected variables.Logistic regression was used to develop the liver operation pulmonary complication scoring system(LOPCSS)for the prediction of PPCs.The LOPCSS was verified using the receiver operating characteristic curve.RESULTS According to the multivariate correlation analysis,the independent factors which influenced PPCs of liver surgery were age[≥65 years old/<65 years old,odds ratio(OR)=1.926,P=0.011],medical diseases requiring drug treatment(yes/no,OR=3.523,P<0.001),number of liver segments to be removed(≥3/≤2,OR=1.683,P=0.002),operation duration(≥180 min/<180 min,OR=1.896,P=0.004),and blood transfusion(yes/no,OR=1.836,P=0.003).The area under the curve(AUC)of the LOPCSS was 0.742.The cut-off value of the expected score for complications was 5.The incidence of complications in the group with≤4 points was significantly lower than that in the group with≥6 points(2.95%vs 33.40%,P<0.001).Furthermore,in the validation dataset,the corresponding AUC of LOPCSS was 0.767.CONCLUSION As a novel and simplified assessment system,the LOPCSS can effectively predict PPCs of liver surgery through perioperative variables.展开更多
Objective:Red blood cell distribution width(RDW)has been utilized as a prognostic indicator for mortality risk assessment in cardiovascular and cerebrovascular patients.Nevertheless,the prognostic significance of RDW ...Objective:Red blood cell distribution width(RDW)has been utilized as a prognostic indicator for mortality risk assessment in cardiovascular and cerebrovascular patients.Nevertheless,the prognostic significance of RDW in critically ill patients with cerebral infarction is yet to be investigated.The objective of this study is to examine the association between RDW and the risk of all-cause mortality in cerebral infarction patients admitted to the intensive care unit(ICU).Method:A retrospective cohort study was conducted using the Medical Information Mart for Intensive Care IV 2.2(MIMIC-IV)intensive care dataset for data analysis.The main results were the all-cause mortality rates at 3 and 12 months of follow-up.Cumulative curves were plotted using the Kaplan-Meier method,and Cox proportional hazards analysis was used to examine the relationship between RDW and mortality rates in critically ill cerebral infarction patients.Results:The findings indicate that RDW serves as a significant prognostic factor for mortality risk in critically ill stroke patients,specifically at the 3 and 12-month follow-up periods.The observed correlation between increasing RDW levels and higher mortality rates among cerebral infarction patients further supports the potential utility of RDW as a predictive indicator.Conclusion:RDW emerges as an independent predictor of mortality risk during the 3 and 12-month follow-up periods for critically ill patients with cerebral infarction.展开更多
BACKGROUND Chronic hepatitis B is a highly heterogeneous disease that can be divided into four phases: Immune tolerant(IT), immune active(IA), inactive carrier(IC) and hepatitis B envelope antigen(HBeAg)-negative hepa...BACKGROUND Chronic hepatitis B is a highly heterogeneous disease that can be divided into four phases: Immune tolerant(IT), immune active(IA), inactive carrier(IC) and hepatitis B envelope antigen(HBeAg)-negative hepatitis(ENEG).AIM To investigate the immune status of natural killer(NK) and T cells in different phases of chronic hepatitis B.METHODS The frequency, phenotype and function of circulating NK cells, as well as nonantigen-specific and hepatitis B virus(HBV)-specific T cell responses were detected by flow cytometry in healthy and HBV-infected subjects.RESULTS The ability of NK cells to produce IFN-γ was markedly attenuated in HBVinfected patients overall but was less compromised in IC patients. Patients in the IT and IA phases also displayed significantly lower TNF-α production compared to healthy subjects. NK cells were phenotypically activated in the IA and ENEGphases, as evidenced by the upregulation of NKp44 in CD56^(bright) NK cells and CD69 in CD56^(dim) NK cells. Furthermore, global T-cells from the ENEG phase displayed a proinflammatory cytokine profile with upregulated IFN-γ and TNF-αexpression, while this profile was suppressed in IT and IA patients. Finally, core and S antigen-specific T cell responses were significantly stronger after in vitro expansion in the IC phase compared to other phases.CONCLUSION Our findings demonstrate the changes in immune response pattern during the natural history of HBV infection. Both NK and T cells are functionally impaired in the IT and IA phases. With the spontaneous clearance of HBeAg and hepatitis B surface antigen decline, NK cell cytokine production and HBV-specific T responses are partially restored in IC phase, and the ENEG phase is dominated by nonantigen-specific T cell responses.展开更多
AIM： To analyze the biological role of the surface antigen of Toxoplasma gondii（Tgondii） in development of vaccine. METHODS： The surface antigen of Tgondii （SAG1） was expressed in vitro. The immune response of t...AIM： To analyze the biological role of the surface antigen of Toxoplasma gondii（Tgondii） in development of vaccine. METHODS： The surface antigen of Tgondii （SAG1） was expressed in vitro. The immune response of the host to the antigen was investigated by detection of specific antibody reaction to SAG1 and production of cytokines. Mice were immunized with recombinant SAG1 and challenged with lethal strain of Tgondii RH. The monoclonal antibody to r-SAG1 was prepared and used to study the effects of SAG1 on Tgondii tachyzoites under electromicroscope. RESULTS： The mice immunized with recombinant SAG1 delayed death for 60 h compared to the control group. The recombinant SAG1 induced specific high titer of IgG and IgM antibodies as well as IFN-y, IL-2 and IL-4 cytokines in mice. In contrast, IL-12, IL-6 and TNF-α were undetectable. When T gondii tachyzoites were treated with the monoclonal antibody to r-SAG1, the parasites were gathered together, destroyed, deformed, swollen, and holes and gaps formed on the surface. CONCLUSION： SAG1 may be an excellent vaccine candidate against T gondii. The immune protection induced by SAG1 against Tgondii may be regulated by both hormone- and cell-mediated immune response.展开更多
Objective This study aims to investigate the effect ofω-3 fatty acid immunonutritional therapy on natural killer(NK)cell gene methylation and function in elderly patients with gastric cancer.Methods A total of 70 cas...Objective This study aims to investigate the effect ofω-3 fatty acid immunonutritional therapy on natural killer(NK)cell gene methylation and function in elderly patients with gastric cancer.Methods A total of 70 cases of elderly patients with gastric cancer were randomized into theω-3 fatty acid group and placebo group,according to the type of nutritional support administered.The methylation status of the tumor necrosis factor(TNF)-αgene promoter in peripheral NK cells was detected by methylation specific polymerase chain reaction,and the TNF-αlevel in peripheral NK cells was detected by enzyme-linked immunosorbent assay.Results After 14 days of immunonutritional therapy withω-3 fatty acid or placebo,patients in theω-3 group had significantly higher average NK cell activity(0.27 vs.0.24,P=0.013)and lower percentages of TNF-αgene promoter methylation(25.7%vs.60%,P<0.05)than the placebo group.However,there were no significant differences in the concentration of albumin,prealbumin and TNF-αin serum,and the NK cell count between theω-3 group and placebo group.Conclusion The postoperative application ofω-3 fatty acid may improve the activity of NK cells,which is correlated to the methylation status of the TNF-αgene promoter.展开更多
Background: The Xingnao Kaiqiao acupuncture method, founded by Academician ShiXuemin, has outstanding efficacy in the treatment of ischemic stroke and has been widelyused at home and abroad. However, after half a cent...Background: The Xingnao Kaiqiao acupuncture method, founded by Academician ShiXuemin, has outstanding efficacy in the treatment of ischemic stroke and has been widelyused at home and abroad. However, after half a century of animal experiments and clinicalstudies, clearly and intuitively revealing its therapeutic mechanism is still a great challengefor researchers. Therefore, this experiment is based on the combination of medicine andengineering to study the immediate effects of the acupuncture method in treating patientsby recording the electroencephalography (EEG) activities of subjects during the acupunctureprocess and to further reveal the therapeutic mechanism at the brain level. Methods: Thistrial is an exploratory, prospective, single-arm interventional study involving a total ofpatients with ischemic stroke. Physicians will record EEG data from patients duringacupuncture as the primary outcome indicator. After pre-processing the EEG data,researchers will use various methods to analyze the immediate effects of acupuncture toobtain brain effectiveness. Deep learning will then be used to identify acupoint stimuli andreceive correspondence between the acupuncture effect and the brain’s internal state.National Institutes of Health Stroke Scale score before and after the acupuncture process willbe used as the secondary outcome indicator. Conclusion: This is the first study protocol toapply dynamic changes in EEG to explore a range of mechanisms of action of acupuncture inthe treatment of ischemic stroke. We propose a method to analyze EEG signals ofacupuncture patients. The deep learning model will be applied for supervised training toobtain the compelling relationship between the acupuncture method and internal brainstates, providing a new prospect for the digitization of traditional Chinese medicine.展开更多
Background:Acupuncture for stroke has been endorsed by the World Health Organization,and the Chinese Guidelines for the Diagnosis and Treatment of Acute Ischemic Stroke 2018 lists acupuncture for stroke as a Level II ...Background:Acupuncture for stroke has been endorsed by the World Health Organization,and the Chinese Guidelines for the Diagnosis and Treatment of Acute Ischemic Stroke 2018 lists acupuncture for stroke as a Level II recommendation with Level B evidence.The efficacy of acupuncture in the treatment of acute ischaemic stroke needs to be approved by more high-quality studies.However,there are currently no real-world studies of acupuncture for stroke.Methods:Stroke patients who meet the study criteria and are hospitalized from February 2021 to March 2022 in 23 medical institutions across China,including the First Teaching Hospital of Tianjin University of Chinese Medicine.The number of patients planned to be recruited is 3,000.Due to the impact of COVID-19,we have applied to the competent authorities for an extension(recruit patients until December 2022).Basic patient information and treatment information will be registered at admission,at discharge,and on the following dates after the onset of the disease:90±7 days,180±7 days,and 360±7 days after the onset of the disease.Establish a database for statistical analysis.Discussion:This study proposes to conduct a prospective cohort study of acupuncture intervention for stroke in a real-world medical setting.Analysis of the effect pattern of acupuncture intervention on the recovery of neurological function system and swallowing disorder in stroke patients.Evaluate the long-term effects of acupuncture intervention for stroke and recurrence of cardiovascular and cerebrovascular events.展开更多
Objective:To summarize the clinical features,diagnosis,and treatment of neonatal staphylococcal scalded skin syndrome(SSSS).Methods:The clinical data with SSSS was analyzed,and the related literature was reviewed.Resu...Objective:To summarize the clinical features,diagnosis,and treatment of neonatal staphylococcal scalded skin syndrome(SSSS).Methods:The clinical data with SSSS was analyzed,and the related literature was reviewed.Results:The acute onset of the disease was characterized by generalized erythema,epidermis exfoliation,skin non-touch,radial chapping around the mouth,and positive Nissl sign.The culture of binocular secretions and neck exudates showed Staphylococcus aureus and was diagnosed as SSSS.According to the results of drug sensitivity of secretions and exudates,vancomycin was selected for anti-infective treatment,and skincare and symptomatic support were given simultaneously.The child was cured and discharged after ten days of treatment.Conclusion:SSSS belongs to neonatal acute and critical illness.Improving etiological examination and timely targeted anti-infective treatment is the key to correct diagnosis and recovery.展开更多
文摘AIM： To evaluate the multi-step pretargeting radioimmunoimaging （RII） and radioimmunotherapy （RTT） in nude mice bearing human colon carcinoma with avidin-biotin system labeled with 153^Sm. METHODS： Two- and three-step strategies for avidinbiotin system pretargeting techniques were established. In a three-step procedure, human colon carcinoma bearing nude mice were first injected with biotinylated monoclonal antibody （McAb-Bt） followed by cold avidin （Av） 48 h later and then 153^Sm-DB2 24 h thereafter; whereas the twostep procedure consisted of injection of 153^Sm-SA 48 h after pretargeting with biotinylated anti-CEA monoclonal antibody （CEA McAb-Bt）. SPECT imaging and biodistribution were performed at 4, 24, 48, or 72 h after injection of 153^Sm-labeled compounds. Five groups of nude mice subcutaneously grafted with human colon carcinoma were treated 3 d after grafting. One group received the injection with 100 μg CEA McAb-Bt followed by cold avidin （80 μg） after 2 d and 11.1 MBCl I53Sm-DB2 after 1 d. Four control groups were treated respectively with 11.1 MBq 153^Sm- CEA McAb, ii.i MBq 153^Sm-nmIgG, ii.i MBq 153^Sm-DB2, 100 μL normal saline. Toxicity was evaluated by changes of leukocyte count, and the efficacy by variation in tumor volume. Histological analyses of tumors were performed. RESULTS： The three-step procedure allowed faster blood clearance and yielded higher tumor blood ratios （5.76 at 4 h and 12.94 at 24 h） of the 153^Sm-DB2. The tumor was clearly visualized at 4 h in y-imaging after the injection of 153^Sm-DB2, while a significant accumulation of 153^Sm-SA in the tumor was observed only 24 h after the injection and tumor blood ratios at 4 and 24 h were 1.00 and 2.03, respectively, in the two-step procedure. Pretargeting RIT and 153^Sm-CEA McAb had a strong tumor-inhibiting effect.The tumor inhibitory rate was 80.67% and 78.44%, respectively, five weeks after therapy. Histopathological evidence also indicated radioactive damage in tumor tissues as necrosis of tumor cells, while in the other organs such as liver and kidney no radioactive damage was observed. Leukocyte counts showed significant decrease after treatment in groups of 153^Sm-CEA McAb and 153^Sm- nmIgG. CONCLUSION： The two kinds of pretargeting strategies can elevate the target-to-nontarget ratio, decrease the blood background and shorten the imaging time compared to 153^Sm-CEA McAb. Three-step pretargeting RIT is as effident as 153^Sm-CEA McAb, but markedly less toxic. This study provides experimental evidence for the clinical application of pretargeting RII and RIT.
文摘Objective At present,there is no appropriate system to evaluate the severe complications of liver surgery through the preoperative factors.This study aimed to design and verify a risk assessment system for the prediction of severe post-operative complications after a hepatectomy based on the preoperative parameters.Methods A retrospective analysis was performed on 1732 patients who had undergone liver surgery.The severity of the complications was graded by Accordion Severity Grading of post-operative complications.The variables were screened by multivariate analysis,and graded scores were assigned to the selected variables.A logistic regression equation was used to form the liver operation risk formula(LORF)for the prediction of severe post-operative complications.The LORF was verified by the receiver operating characteristic(ROC)curve.Results The multivariate correlation analysis revealed the independent influencing factors of the severe post-operative complications of liver surgery were Child-Pugh grade(OR=4.127;P<0.001),medical diseases requiring drug treatment(OR=3.092;P<0.001),the number of liver segments to be removed(OR=2.209;P=0.006),organ invasion(OR=4.538;P=0.024),and pathological type(OR=4.023;P=0.002).The binomial logistic regression model was established to obtain the calculation formula(LORF)of the severe complication risk.The area under the ROC curve(AUC)of the LORF was 0.815.The cut-off value of the expected probability of severe complications was 0.3225(32.25%).Furthermore,in the validation data set,the corresponding AUC of the LORF was 0.829.Conclusion As a novel and simplified assessment system,the LORF could effectively predict the severe post-operative complications of liver surgery through the preoperative factors,and therefore it could be used to evaluate the risk of severe liver surgical complications before surgery.
文摘BACKGROUND At present,there is no perfect system to evaluate pulmonary complications of liver surgery using perioperative variables.AIM To design and verify a risk assessment system for predicting postoperative pulmonary complications(PPCs)after hepatectomy based on perioperative variables.METHODS A retrospective analysis was performed on 1633 patients who underwent liver surgery.The variables were screened using univariate and multivariate analyses,and graded scores were assigned to the selected variables.Logistic regression was used to develop the liver operation pulmonary complication scoring system(LOPCSS)for the prediction of PPCs.The LOPCSS was verified using the receiver operating characteristic curve.RESULTS According to the multivariate correlation analysis,the independent factors which influenced PPCs of liver surgery were age[≥65 years old/<65 years old,odds ratio(OR)=1.926,P=0.011],medical diseases requiring drug treatment(yes/no,OR=3.523,P<0.001),number of liver segments to be removed(≥3/≤2,OR=1.683,P=0.002),operation duration(≥180 min/<180 min,OR=1.896,P=0.004),and blood transfusion(yes/no,OR=1.836,P=0.003).The area under the curve(AUC)of the LOPCSS was 0.742.The cut-off value of the expected score for complications was 5.The incidence of complications in the group with≤4 points was significantly lower than that in the group with≥6 points(2.95%vs 33.40%,P<0.001).Furthermore,in the validation dataset,the corresponding AUC of LOPCSS was 0.767.CONCLUSION As a novel and simplified assessment system,the LOPCSS can effectively predict PPCs of liver surgery through perioperative variables.
基金Project of Science and Technology Plan of Tianjin City(Grant number 20ZYJDSY00020)。
文摘Objective:Red blood cell distribution width(RDW)has been utilized as a prognostic indicator for mortality risk assessment in cardiovascular and cerebrovascular patients.Nevertheless,the prognostic significance of RDW in critically ill patients with cerebral infarction is yet to be investigated.The objective of this study is to examine the association between RDW and the risk of all-cause mortality in cerebral infarction patients admitted to the intensive care unit(ICU).Method:A retrospective cohort study was conducted using the Medical Information Mart for Intensive Care IV 2.2(MIMIC-IV)intensive care dataset for data analysis.The main results were the all-cause mortality rates at 3 and 12 months of follow-up.Cumulative curves were plotted using the Kaplan-Meier method,and Cox proportional hazards analysis was used to examine the relationship between RDW and mortality rates in critically ill cerebral infarction patients.Results:The findings indicate that RDW serves as a significant prognostic factor for mortality risk in critically ill stroke patients,specifically at the 3 and 12-month follow-up periods.The observed correlation between increasing RDW levels and higher mortality rates among cerebral infarction patients further supports the potential utility of RDW as a predictive indicator.Conclusion:RDW emerges as an independent predictor of mortality risk during the 3 and 12-month follow-up periods for critically ill patients with cerebral infarction.
基金Supported by National Science Fund of China(NSFC)No.30771911National Science and Technology Major Project No.2012ZX10002007-003
文摘BACKGROUND Chronic hepatitis B is a highly heterogeneous disease that can be divided into four phases: Immune tolerant(IT), immune active(IA), inactive carrier(IC) and hepatitis B envelope antigen(HBeAg)-negative hepatitis(ENEG).AIM To investigate the immune status of natural killer(NK) and T cells in different phases of chronic hepatitis B.METHODS The frequency, phenotype and function of circulating NK cells, as well as nonantigen-specific and hepatitis B virus(HBV)-specific T cell responses were detected by flow cytometry in healthy and HBV-infected subjects.RESULTS The ability of NK cells to produce IFN-γ was markedly attenuated in HBVinfected patients overall but was less compromised in IC patients. Patients in the IT and IA phases also displayed significantly lower TNF-α production compared to healthy subjects. NK cells were phenotypically activated in the IA and ENEGphases, as evidenced by the upregulation of NKp44 in CD56^(bright) NK cells and CD69 in CD56^(dim) NK cells. Furthermore, global T-cells from the ENEG phase displayed a proinflammatory cytokine profile with upregulated IFN-γ and TNF-αexpression, while this profile was suppressed in IT and IA patients. Finally, core and S antigen-specific T cell responses were significantly stronger after in vitro expansion in the IC phase compared to other phases.CONCLUSION Our findings demonstrate the changes in immune response pattern during the natural history of HBV infection. Both NK and T cells are functionally impaired in the IT and IA phases. With the spontaneous clearance of HBeAg and hepatitis B surface antigen decline, NK cell cytokine production and HBV-specific T responses are partially restored in IC phase, and the ENEG phase is dominated by nonantigen-specific T cell responses.
基金Supported by China Ministry of Human Affairs and Department of Science and Technology of Shandong Province, No. 031050115
文摘AIM： To analyze the biological role of the surface antigen of Toxoplasma gondii（Tgondii） in development of vaccine. METHODS： The surface antigen of Tgondii （SAG1） was expressed in vitro. The immune response of the host to the antigen was investigated by detection of specific antibody reaction to SAG1 and production of cytokines. Mice were immunized with recombinant SAG1 and challenged with lethal strain of Tgondii RH. The monoclonal antibody to r-SAG1 was prepared and used to study the effects of SAG1 on Tgondii tachyzoites under electromicroscope. RESULTS： The mice immunized with recombinant SAG1 delayed death for 60 h compared to the control group. The recombinant SAG1 induced specific high titer of IgG and IgM antibodies as well as IFN-y, IL-2 and IL-4 cytokines in mice. In contrast, IL-12, IL-6 and TNF-α were undetectable. When T gondii tachyzoites were treated with the monoclonal antibody to r-SAG1, the parasites were gathered together, destroyed, deformed, swollen, and holes and gaps formed on the surface. CONCLUSION： SAG1 may be an excellent vaccine candidate against T gondii. The immune protection induced by SAG1 against Tgondii may be regulated by both hormone- and cell-mediated immune response.
基金This project was supported by the General Project of Natural Science Foundation of Hubei Province(No.2020CFB826).
文摘Objective This study aims to investigate the effect ofω-3 fatty acid immunonutritional therapy on natural killer(NK)cell gene methylation and function in elderly patients with gastric cancer.Methods A total of 70 cases of elderly patients with gastric cancer were randomized into theω-3 fatty acid group and placebo group,according to the type of nutritional support administered.The methylation status of the tumor necrosis factor(TNF)-αgene promoter in peripheral NK cells was detected by methylation specific polymerase chain reaction,and the TNF-αlevel in peripheral NK cells was detected by enzyme-linked immunosorbent assay.Results After 14 days of immunonutritional therapy withω-3 fatty acid or placebo,patients in theω-3 group had significantly higher average NK cell activity(0.27 vs.0.24,P=0.013)and lower percentages of TNF-αgene promoter methylation(25.7%vs.60%,P<0.05)than the placebo group.However,there were no significant differences in the concentration of albumin,prealbumin and TNF-αin serum,and the NK cell count between theω-3 group and placebo group.Conclusion The postoperative application ofω-3 fatty acid may improve the activity of NK cells,which is correlated to the methylation status of the TNF-αgene promoter.
基金The research was supported by the Department of Science and Technology of the National Administration of Traditional Chinese Medicine Local Project of Central Financial Transfer Payment in 2021(grant no.2021JDBZTS-01).
文摘Background: The Xingnao Kaiqiao acupuncture method, founded by Academician ShiXuemin, has outstanding efficacy in the treatment of ischemic stroke and has been widelyused at home and abroad. However, after half a century of animal experiments and clinicalstudies, clearly and intuitively revealing its therapeutic mechanism is still a great challengefor researchers. Therefore, this experiment is based on the combination of medicine andengineering to study the immediate effects of the acupuncture method in treating patientsby recording the electroencephalography (EEG) activities of subjects during the acupunctureprocess and to further reveal the therapeutic mechanism at the brain level. Methods: Thistrial is an exploratory, prospective, single-arm interventional study involving a total ofpatients with ischemic stroke. Physicians will record EEG data from patients duringacupuncture as the primary outcome indicator. After pre-processing the EEG data,researchers will use various methods to analyze the immediate effects of acupuncture toobtain brain effectiveness. Deep learning will then be used to identify acupoint stimuli andreceive correspondence between the acupuncture effect and the brain’s internal state.National Institutes of Health Stroke Scale score before and after the acupuncture process willbe used as the secondary outcome indicator. Conclusion: This is the first study protocol toapply dynamic changes in EEG to explore a range of mechanisms of action of acupuncture inthe treatment of ischemic stroke. We propose a method to analyze EEG signals ofacupuncture patients. The deep learning model will be applied for supervised training toobtain the compelling relationship between the acupuncture method and internal brainstates, providing a new prospect for the digitization of traditional Chinese medicine.
基金supported by the Tianjin Municipal Science and Technology Bureau.Grant number(No.20ZYJDSY00020).
文摘Background:Acupuncture for stroke has been endorsed by the World Health Organization,and the Chinese Guidelines for the Diagnosis and Treatment of Acute Ischemic Stroke 2018 lists acupuncture for stroke as a Level II recommendation with Level B evidence.The efficacy of acupuncture in the treatment of acute ischaemic stroke needs to be approved by more high-quality studies.However,there are currently no real-world studies of acupuncture for stroke.Methods:Stroke patients who meet the study criteria and are hospitalized from February 2021 to March 2022 in 23 medical institutions across China,including the First Teaching Hospital of Tianjin University of Chinese Medicine.The number of patients planned to be recruited is 3,000.Due to the impact of COVID-19,we have applied to the competent authorities for an extension(recruit patients until December 2022).Basic patient information and treatment information will be registered at admission,at discharge,and on the following dates after the onset of the disease:90±7 days,180±7 days,and 360±7 days after the onset of the disease.Establish a database for statistical analysis.Discussion:This study proposes to conduct a prospective cohort study of acupuncture intervention for stroke in a real-world medical setting.Analysis of the effect pattern of acupuncture intervention on the recovery of neurological function system and swallowing disorder in stroke patients.Evaluate the long-term effects of acupuncture intervention for stroke and recurrence of cardiovascular and cerebrovascular events.
文摘Objective:To summarize the clinical features,diagnosis,and treatment of neonatal staphylococcal scalded skin syndrome(SSSS).Methods:The clinical data with SSSS was analyzed,and the related literature was reviewed.Results:The acute onset of the disease was characterized by generalized erythema,epidermis exfoliation,skin non-touch,radial chapping around the mouth,and positive Nissl sign.The culture of binocular secretions and neck exudates showed Staphylococcus aureus and was diagnosed as SSSS.According to the results of drug sensitivity of secretions and exudates,vancomycin was selected for anti-infective treatment,and skincare and symptomatic support were given simultaneously.The child was cured and discharged after ten days of treatment.Conclusion:SSSS belongs to neonatal acute and critical illness.Improving etiological examination and timely targeted anti-infective treatment is the key to correct diagnosis and recovery.
