Villin(VLN)is considered to be one of the most important actin-binding proteins,participates in modulating the actin cytoskeleton dynamics,plays essential role in plant development and resisting adverse environments.H...Villin(VLN)is considered to be one of the most important actin-binding proteins,participates in modulating the actin cytoskeleton dynamics,plays essential role in plant development and resisting adverse environments.However,systematic studies of the VLN gene family have not been reported in cotton(Gossypium).In this study,14 GhVLNs were identified in G.hirsutum.These GhVLN genes were distributed in 6 A-subgenome chromosomes and 6 D-subgenome chromosomes of the allotetraploid upland cotton and classified into three phylogenetical groups based on the classification model of AtVLNs.In addition,the 14 GhVLN genes have highly conserved gene structure and motif architecture.The number of introns was ranged from 18 to 22 and the length of protein sequences was varied from 901 to 1077.Six gelsolin homology domains,G1–G6,and villin headpiece domain,VHP,were existed in all GhVLNs with the exception of two VLNs(GhVLN6 and GhVLN13)which lacked VHP.Cis-elements analysis revealed that the promoter regions of GhVLNs contained various light related components and also elements responsible for phytohormones and stresses response,indicating that,when subjected to those adverse environments,cotton plants may activate the response system by targeting VLN genes to survive the crisis.Heatmaps showed that the GhVLN genes exhibited various expression patterns,some were accumulated in certain tissues,root,petal,stamen or elongating fibers,and some were obviously induced by environmental changes.Especially GhVLN3 and GhVLN10 were highly and preferentially expressed in elongating fibers and distinctly upregulated by abiotic(salt,PEG,cold and heat)and biotic(Verticillium dahliae V991)stresses.This study may provide useful information for biological function identification of GhVLN genes and gene resources for creating high-quality and various resistant cotton germplasms.展开更多
The weak heat tolerance of Clematis ornamental varieties negatively affects their ornamental qualities in the summer.To elucidate heat resistance mechanisms,Clematis lanuginosa,which is an important original parent of...The weak heat tolerance of Clematis ornamental varieties negatively affects their ornamental qualities in the summer.To elucidate heat resistance mechanisms,Clematis lanuginosa,which is an important original parent of the Clematis large-flowered group of ornamental varieties,was selected for use in this study.Here,six libraries,including three biological replicates each of control and heat-shock stress samples,were determined using RNA-sequencing technology.In total,62,050 unigenes were obtained,and 6,439 unigenes exceeded 1 kb in length.A total of 42,377 unigenes were annotated using six databases.Between the two treatments,2,165 differentially expressed genes were identified,with 1,565 being up-regulated and 600 down-regulated.In addition,51 heat-shock protein-encoding genes were identified,among which the small heat-shock proteins accounted for 68.63%.In total,two heat-shock factors and 12 ribosomal proteins were significantly up-regulated under heatstress conditions.The differential expressions of ethylene-responsive transcription factor,chalcone synthase,cysteine-rich receptor-like kinase and aspartic protease unigenes in guard cells were induced by heat-shock.The data obtained will assist the elucidation of the molecular events underlying heat-stress responses in C.lanuginosa.展开更多
基金This work was financially supported by the National Natural Science Foundation of China(No.31801408)the Natural Science Foundation of Jiangsu Province,China(No.BK20180517).
文摘Villin(VLN)is considered to be one of the most important actin-binding proteins,participates in modulating the actin cytoskeleton dynamics,plays essential role in plant development and resisting adverse environments.However,systematic studies of the VLN gene family have not been reported in cotton(Gossypium).In this study,14 GhVLNs were identified in G.hirsutum.These GhVLN genes were distributed in 6 A-subgenome chromosomes and 6 D-subgenome chromosomes of the allotetraploid upland cotton and classified into three phylogenetical groups based on the classification model of AtVLNs.In addition,the 14 GhVLN genes have highly conserved gene structure and motif architecture.The number of introns was ranged from 18 to 22 and the length of protein sequences was varied from 901 to 1077.Six gelsolin homology domains,G1–G6,and villin headpiece domain,VHP,were existed in all GhVLNs with the exception of two VLNs(GhVLN6 and GhVLN13)which lacked VHP.Cis-elements analysis revealed that the promoter regions of GhVLNs contained various light related components and also elements responsible for phytohormones and stresses response,indicating that,when subjected to those adverse environments,cotton plants may activate the response system by targeting VLN genes to survive the crisis.Heatmaps showed that the GhVLN genes exhibited various expression patterns,some were accumulated in certain tissues,root,petal,stamen or elongating fibers,and some were obviously induced by environmental changes.Especially GhVLN3 and GhVLN10 were highly and preferentially expressed in elongating fibers and distinctly upregulated by abiotic(salt,PEG,cold and heat)and biotic(Verticillium dahliae V991)stresses.This study may provide useful information for biological function identification of GhVLN genes and gene resources for creating high-quality and various resistant cotton germplasms.
基金supported by the National Natural Science Foundation of China(31800603)the Jiangsu Agricultural Science and Technology Innovation Fund[CX(20)3027]Jiangsu Key Laboratory for the Research and Utilization of Plant Resources(JSPKLB202203).
文摘The weak heat tolerance of Clematis ornamental varieties negatively affects their ornamental qualities in the summer.To elucidate heat resistance mechanisms,Clematis lanuginosa,which is an important original parent of the Clematis large-flowered group of ornamental varieties,was selected for use in this study.Here,six libraries,including three biological replicates each of control and heat-shock stress samples,were determined using RNA-sequencing technology.In total,62,050 unigenes were obtained,and 6,439 unigenes exceeded 1 kb in length.A total of 42,377 unigenes were annotated using six databases.Between the two treatments,2,165 differentially expressed genes were identified,with 1,565 being up-regulated and 600 down-regulated.In addition,51 heat-shock protein-encoding genes were identified,among which the small heat-shock proteins accounted for 68.63%.In total,two heat-shock factors and 12 ribosomal proteins were significantly up-regulated under heatstress conditions.The differential expressions of ethylene-responsive transcription factor,chalcone synthase,cysteine-rich receptor-like kinase and aspartic protease unigenes in guard cells were induced by heat-shock.The data obtained will assist the elucidation of the molecular events underlying heat-stress responses in C.lanuginosa.
