The concept of labor division and multi-module cooperation of microbial consortia offers it promising potentials in various areas,such as the utilization of complex substrates,synthesis of natural compounds with long ...The concept of labor division and multi-module cooperation of microbial consortia offers it promising potentials in various areas,such as the utilization of complex substrates,synthesis of natural compounds with long metabolic pathways and remediation of environmental pollutants within a hostile environment.Consequently,synthetic microbial consortia represent a new frontier for synthetic biology because they can solve more complex problems than monocultures.However,current research on microbial consortia often involves the simple mixing of multiphase systems,where strains are co-cultured sequentially or individually cultured and then mixed-cultured.The instability and low efficiency of microbial consortia systems hindered their practical application.To construct a stable and efficient microbial consortium,it is essential to consider the different growth and metabolic characteristics of strains,the competition for various nutrients as well as the complex carbon,energy and signaling dynamics within the system.In this review,we provide a progressive strategy for constructing a stable and efficient microbial consortium system across three stages:compromised stage(work together),microenvironment-oriented stage(work better),and metabolite delivery-enhanced stage(work best).The detailed methods and points for attention of each stage are summarized,with a highlight on the technical bottleneck and application limitations.Through the integration of interdisciplinary strategies,such as materials science and mathematical models,the goal of building a stable and efficient microbial consortium is constantly advanced.展开更多
L-malate is an intermediate of the tricarboxylic acid cycle which is naturally occurred in various microorganisms,and it has been widely applied in polymer,beverage and food,textile,agricultural and pharmaceutical ind...L-malate is an intermediate of the tricarboxylic acid cycle which is naturally occurred in various microorganisms,and it has been widely applied in polymer,beverage and food,textile,agricultural and pharmaceutical industries.Driven by the pursuit of a sustainable economy,microbial production of L-malate has received much attention in last decades.In this review,we focus on the utilization of wastes and/or byproducts as feedstocks for the microbial production of L-malate.Firstly,we present the recent developments on the natural or engineered metabolic pathways that dedicate to the biosynthesis of L-malate,and also provide a comprehensive discussions on developing high-efficient producers.Then,the recent achievements in microbial production of L-malate from various carbon sources were concluded and discussed.Furthermore,some abundant non-food feedstocks which have been used for microbial production of other chemicals were reviewed,as they may be potential candidate feedstock for L-malate production in future.Finally,we outlined the major challenges and proposed further improvements for the production of L-malate.展开更多
Palmitoleic acid(POA)can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry.Recently,the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified...Palmitoleic acid(POA)can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry.Recently,the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid(13.80%).In this study,process optimization focused on dissolved oxygen regulation to improve POA production was conducted.Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation.Under the best condition of 1000 r·min^(-1)of agitation and 1 vvm(airvolume/culture volume/min)of aeration,no ethanol was detected during the whole fermentation process,while a dry biomass concentration of 44.80 g·L^(-1)with 13.43 g·L^(-1)of lipid and 2.93 g·L^(-1)of POA was achieved.Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation,while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.展开更多
Artificial multi-enzyme cascades bear great potential for bioconversion of C1 compounds to value-added chemicals.Over the past decade,massive efforts have been devoted to constructing multi-enzyme cascades to produce ...Artificial multi-enzyme cascades bear great potential for bioconversion of C1 compounds to value-added chemicals.Over the past decade,massive efforts have been devoted to constructing multi-enzyme cascades to produce glycolic acid,rare functional sugars and even starch from C1 compounds.However,in contrast to traditional fermentation utilizing C1 compounds with the expectation of competitive economic performance in future industrialization,multi-enzyme cascades systems in the proof-of-concept phase are facing the challenges of upscaling.Here,we offered an overview of the recent advances in the construction of in vitro multi-enzyme cascades and whole-cell transformation using C1 compounds as substrate.In addition,the existing challenges and possible solutions were also discussed aiming to combine the strengths of in vitro and in vivo multi-enzyme cascades systems for upscaling.展开更多
基金supported by the National Key Research and Development Program of China(2022YFC2104800)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTD2205)the State Key Laboratory of Materials-Oriented Chemical Engineering(KL-MCE-23A10).
文摘The concept of labor division and multi-module cooperation of microbial consortia offers it promising potentials in various areas,such as the utilization of complex substrates,synthesis of natural compounds with long metabolic pathways and remediation of environmental pollutants within a hostile environment.Consequently,synthetic microbial consortia represent a new frontier for synthetic biology because they can solve more complex problems than monocultures.However,current research on microbial consortia often involves the simple mixing of multiphase systems,where strains are co-cultured sequentially or individually cultured and then mixed-cultured.The instability and low efficiency of microbial consortia systems hindered their practical application.To construct a stable and efficient microbial consortium,it is essential to consider the different growth and metabolic characteristics of strains,the competition for various nutrients as well as the complex carbon,energy and signaling dynamics within the system.In this review,we provide a progressive strategy for constructing a stable and efficient microbial consortium system across three stages:compromised stage(work together),microenvironment-oriented stage(work better),and metabolite delivery-enhanced stage(work best).The detailed methods and points for attention of each stage are summarized,with a highlight on the technical bottleneck and application limitations.Through the integration of interdisciplinary strategies,such as materials science and mathematical models,the goal of building a stable and efficient microbial consortium is constantly advanced.
基金This work was supported by the National Key R&D Program of China(2018YFA0901500)the National Natural Science Foundation of China(21706124,21727818)+1 种基金the Key Science and Technology Project of Jiangsu Province(BE2016389)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture of China.
文摘L-malate is an intermediate of the tricarboxylic acid cycle which is naturally occurred in various microorganisms,and it has been widely applied in polymer,beverage and food,textile,agricultural and pharmaceutical industries.Driven by the pursuit of a sustainable economy,microbial production of L-malate has received much attention in last decades.In this review,we focus on the utilization of wastes and/or byproducts as feedstocks for the microbial production of L-malate.Firstly,we present the recent developments on the natural or engineered metabolic pathways that dedicate to the biosynthesis of L-malate,and also provide a comprehensive discussions on developing high-efficient producers.Then,the recent achievements in microbial production of L-malate from various carbon sources were concluded and discussed.Furthermore,some abundant non-food feedstocks which have been used for microbial production of other chemicals were reviewed,as they may be potential candidate feedstock for L-malate production in future.Finally,we outlined the major challenges and proposed further improvements for the production of L-malate.
基金supported by the National Key Research & Development Program of China (2021YFC2101500, 2018YFA0902200)National Natural Science Foundation of China (22008115, 21978130)+4 种基金Jiangsu Province Natural Science Foundation for Youths (SBK2020044721)Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund Project (CX(21)3120)Jiangsu Planned Projects for Postdoctoral Research Funds (2021K085A)China Postdoctoral Science Foundation (2020M671467)Postdoctoral Research Funding Program of Jiangsu Province (2021K085A)
文摘Palmitoleic acid(POA)can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry.Recently,the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid(13.80%).In this study,process optimization focused on dissolved oxygen regulation to improve POA production was conducted.Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation.Under the best condition of 1000 r·min^(-1)of agitation and 1 vvm(airvolume/culture volume/min)of aeration,no ethanol was detected during the whole fermentation process,while a dry biomass concentration of 44.80 g·L^(-1)with 13.43 g·L^(-1)of lipid and 2.93 g·L^(-1)of POA was achieved.Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation,while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.
基金supported by National Key R&D Program of China(2022YFC2105900)the National Natural Science Foundation of China(22078151,22378199,21978130)+1 种基金Jiangsu Natural Science Fund for Distinguished Young Scholars(BK20220052)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTD2215)Young Elite Scientist Sponsorship Program by CAST(YESS20200174).
文摘Artificial multi-enzyme cascades bear great potential for bioconversion of C1 compounds to value-added chemicals.Over the past decade,massive efforts have been devoted to constructing multi-enzyme cascades to produce glycolic acid,rare functional sugars and even starch from C1 compounds.However,in contrast to traditional fermentation utilizing C1 compounds with the expectation of competitive economic performance in future industrialization,multi-enzyme cascades systems in the proof-of-concept phase are facing the challenges of upscaling.Here,we offered an overview of the recent advances in the construction of in vitro multi-enzyme cascades and whole-cell transformation using C1 compounds as substrate.In addition,the existing challenges and possible solutions were also discussed aiming to combine the strengths of in vitro and in vivo multi-enzyme cascades systems for upscaling.
