Objective:To summarize the rules of acupoint selection in treatment of cancer with moxibustion by analyzing the articles of relevant clinical trials in recent years in China,so as to provide the references for acupoin...Objective:To summarize the rules of acupoint selection in treatment of cancer with moxibustion by analyzing the articles of relevant clinical trials in recent years in China,so as to provide the references for acupoint selection of moxibustion for cancer.Methods:The articles of clinical trials for treatment of cancer with moxibustion were retrieved from China National knowledge Infrastructure(CNKI),VIP Chinese Journal Service Platform(VIP)and Wanfang Data Knowledge Service Platform(Wanfang)from 1st January 2015 to 30th June 2021.Using EpiData software,the data were extracted and exported in Excel.The analysis was performed with IBM SPSS Statistics 23 software.Results:A total of 516 articles were collected,including 533 prescriptions with 164 acupoints involved.The high-frequency acupoints were similar among 7 types of cancers and these acupoints mostly acted on supporting the antipathogenic qi,replenishing qi and regulating the spleen and stomach.Regarding meridian tropism,the acupoints from the conception vessel,the governor vessel and bladder meridian were dominant in treatment of cancer with moxibustion.In terms of location distribution,the selected acupoints were generally distributed on the chest,abdomen,lumbar region and the upper back of the body.Based on cancer related symtomes in different systems,the use frequency of Zusanli(ST36)was the highest for the symptoms of the immune,digestive,circulatory and motor systems;that of Feishu(BL13)was the highest for the respiratory system symptoms and that of Guanyuan(CV4)was the highest for the urinary system symptoms.The cluster analysis of high-frequency acupoints showed that the acupoints of moxibustion for cancer generally work on cultivating yang,supporting the antipathogenic qi,strengthening the spleen and harmonizing the stomach,and were selected in association with the clinical symptoms of different cancers.展开更多
Objective:The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)were evaluated.Changes in t...Objective:The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)were evaluated.Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated.The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions,thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development.Methods:Sixty healthy male Wistar rats were randomly divided into four groups:Control,control+moxibustion,model,and moxibustion groups.A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG,with a dose of 1 mL/100 g body weight,once daily for 16 weeks.The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling,continuing for 16 weeks.After the experiment,gastric tissue was collected,and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining.Immunohistochemistry(IHC)and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues.Enzyme-linked immunosorbent assays(ELISA)were used to measure the levels of interleukin-12(IL-12),interferon-gamma(IFN-γ),and tumor necrosis factor-beta(TNF-β)in rat serum.Results:Upon macroscopic observation,the gastric mucosa of rats in the control and control+moxibustion groups appeared uniformly red,with a glossy mucosal surface,normal gastric wall elasticity,and clear,regular mucosal folds,without hyperplasia or bleeding points.In the model group,the gastric mucosa was reduced in volume,the gastric wall thinned,elasticity decreased,mucosal folds were disordered,and yellow-white cauliflower-like lesions and yellow-brown hyperkeratosis were observed.In the moxibustion group,the gastric mucosa showed decreased elasticity,with disordered mucosal folds and granular hyperplasia.After H&E staining,the gastric mucosal structure was clear and intact in the control and control+moxibustion groups displaying an organized and uniform arrangement of the mucosa,submucosa,and muscularis propria,without hyperplasia or keratinization.In the model group,the epithelial glands in the gastric mucosa were disordered,with varied cell morphologies,thickened submucosa,and disrupted squamous epithelium that invaded downward into the muscularis propria.In the moxibustion group,the squamous epithelium did not invade the muscularis propria.IHC results showed higher expression levels of CD80 and CD86 in the gastric mucosa of the control+moxibustion group than in the control group(P<0.05)and lower expression levels in the model group than in the control group(P<0.05).The moxibustion group showed higher CD80 and CD86 levels than those in the model group(P<0.05).Western blotting indicated that CD80 and CD86 levels were higher in the moxibustion group than in the model group(P<0.05).ELISA results showed higher IL-12 levels in the model group than in the control group(P<0.05)and higher TNF-βand IFN-γlevels in the moxibustion group than in the model group(P<0.01).Conclusion:Direct moxibustion alleviates the pathological progression of gastric cancer in an MNNGinduced rat model.Its mechanisms may involve effects on the state of antigen-presenting cells,thereby promoting T cell activation and enhancing immune function.展开更多
基金Supported by National Natural Science Foundation of China:81173343Scientific Research Capacity Improvement Project of Hebei University of Chinese Medicine:KTZ2019012+2 种基金Scientific Program of Hebei Administration of Traditional Chinese Medicine:No.2022361Doctoral Research Fund Project of Hebei University of Traditional Chinese Medicine:BSZ2022006Natural Science Foundation of Hebei Province:No.H2023423029。
文摘Objective:To summarize the rules of acupoint selection in treatment of cancer with moxibustion by analyzing the articles of relevant clinical trials in recent years in China,so as to provide the references for acupoint selection of moxibustion for cancer.Methods:The articles of clinical trials for treatment of cancer with moxibustion were retrieved from China National knowledge Infrastructure(CNKI),VIP Chinese Journal Service Platform(VIP)and Wanfang Data Knowledge Service Platform(Wanfang)from 1st January 2015 to 30th June 2021.Using EpiData software,the data were extracted and exported in Excel.The analysis was performed with IBM SPSS Statistics 23 software.Results:A total of 516 articles were collected,including 533 prescriptions with 164 acupoints involved.The high-frequency acupoints were similar among 7 types of cancers and these acupoints mostly acted on supporting the antipathogenic qi,replenishing qi and regulating the spleen and stomach.Regarding meridian tropism,the acupoints from the conception vessel,the governor vessel and bladder meridian were dominant in treatment of cancer with moxibustion.In terms of location distribution,the selected acupoints were generally distributed on the chest,abdomen,lumbar region and the upper back of the body.Based on cancer related symtomes in different systems,the use frequency of Zusanli(ST36)was the highest for the symptoms of the immune,digestive,circulatory and motor systems;that of Feishu(BL13)was the highest for the respiratory system symptoms and that of Guanyuan(CV4)was the highest for the urinary system symptoms.The cluster analysis of high-frequency acupoints showed that the acupoints of moxibustion for cancer generally work on cultivating yang,supporting the antipathogenic qi,strengthening the spleen and harmonizing the stomach,and were selected in association with the clinical symptoms of different cancers.
基金Supported by the National Natural Science Foundation of China:81173342Scientific Research Ability Improvement Projects of Hebei College of Traditional Chinese Medicine(now known as Hebei University of Chinese Medicine):KTZ2019012Scientific Research Project of Hebei Province Administration of Traditional Chinese Medicine:2022361.2021095。
文摘Objective:The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)were evaluated.Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated.The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions,thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development.Methods:Sixty healthy male Wistar rats were randomly divided into four groups:Control,control+moxibustion,model,and moxibustion groups.A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG,with a dose of 1 mL/100 g body weight,once daily for 16 weeks.The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling,continuing for 16 weeks.After the experiment,gastric tissue was collected,and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining.Immunohistochemistry(IHC)and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues.Enzyme-linked immunosorbent assays(ELISA)were used to measure the levels of interleukin-12(IL-12),interferon-gamma(IFN-γ),and tumor necrosis factor-beta(TNF-β)in rat serum.Results:Upon macroscopic observation,the gastric mucosa of rats in the control and control+moxibustion groups appeared uniformly red,with a glossy mucosal surface,normal gastric wall elasticity,and clear,regular mucosal folds,without hyperplasia or bleeding points.In the model group,the gastric mucosa was reduced in volume,the gastric wall thinned,elasticity decreased,mucosal folds were disordered,and yellow-white cauliflower-like lesions and yellow-brown hyperkeratosis were observed.In the moxibustion group,the gastric mucosa showed decreased elasticity,with disordered mucosal folds and granular hyperplasia.After H&E staining,the gastric mucosal structure was clear and intact in the control and control+moxibustion groups displaying an organized and uniform arrangement of the mucosa,submucosa,and muscularis propria,without hyperplasia or keratinization.In the model group,the epithelial glands in the gastric mucosa were disordered,with varied cell morphologies,thickened submucosa,and disrupted squamous epithelium that invaded downward into the muscularis propria.In the moxibustion group,the squamous epithelium did not invade the muscularis propria.IHC results showed higher expression levels of CD80 and CD86 in the gastric mucosa of the control+moxibustion group than in the control group(P<0.05)and lower expression levels in the model group than in the control group(P<0.05).The moxibustion group showed higher CD80 and CD86 levels than those in the model group(P<0.05).Western blotting indicated that CD80 and CD86 levels were higher in the moxibustion group than in the model group(P<0.05).ELISA results showed higher IL-12 levels in the model group than in the control group(P<0.05)and higher TNF-βand IFN-γlevels in the moxibustion group than in the model group(P<0.01).Conclusion:Direct moxibustion alleviates the pathological progression of gastric cancer in an MNNGinduced rat model.Its mechanisms may involve effects on the state of antigen-presenting cells,thereby promoting T cell activation and enhancing immune function.
