Biomedical applications of nanomaterials are exponentially increasing every year due to analogy to various cell receptors, ligands, structural proteins, and genetic materials(that is, DNA). In bone tissue, nanoscale m...Biomedical applications of nanomaterials are exponentially increasing every year due to analogy to various cell receptors, ligands, structural proteins, and genetic materials(that is, DNA). In bone tissue, nanoscale materials can provide scaffold for excellent tissue repair via mechanical stimulation, releasing of various loaded drugs and mediators, 3D scaffold for cell growth and differentiation of bone marrow stem cells to osteocytes. This review will therefore highlight recent advancements on tissue and nanoscale materials interaction.展开更多
Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging metho...Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging method for accurate and rapid diagnosis of cancer cells by introducing aqueous Fe^2＋ and Zn^2＋ ions into cancer cells （i.e., HeLa, U87, and HepG2 cancer cells）. We found that the biocompatible metal ions Fe^2＋ and Zn^2＋ forced the cancer cells to spontaneously synthesize fluorescent ZnO nanoclusters and magnetic Fe3O4 nanoclusters. These clusters could then be used for multimodal cancer imaging by combining fluorescence imaging with magnetic resonance imaging and computed tomography imaging. Meanwhile, for normal cells （i.e., L02） and tissues, neither fluorescence nor any other obvious difference could be detected between pre- and post-injection. This multimodal bioimaging strategy based on the in situ biosynthesized Zn＆Fe oxide nanoclusters might therefore be useful for early cancer diagnosis and therapy.展开更多
Photodynamic therapy （PDT） is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficac...Photodynamic therapy （PDT） is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficacy, e.g. effective concentra- tion of the drug at the desired sites, the irradiation light dosimetry and biocompatibility of the photosensitizer. The introduction of nanotechnology and nanomaterial like biocompatible nano-titania （i.e., nano-TiO2） may facilitate to solve some of these problems. In this study we have explored the possibility of combining tetra sulphonatophenyl porphyrin （TSPP） with nano-titania （PT） for efficient PDT with least adverse effects. The spectroscopic properties of these nano-cornposites were characterized by using fluorescence and UV-Vis absorption spectroscopic study. The singlet oxygen quantum yield was deter- mined by using 2,5-diphenyl-3,4-benzofuran （DPBF）, while the effect of nano TiO2 with TSPP on the synovial fibroblast cells from human （HSC） and rat models （RSC） were investigated by confocal laser microscopy and 3-（4,5-dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay. Our results suggest that nano TiO2 with TSPP can be readily utilized for effec- tive PDT treatment of Rheumatoid Arthritis （RA）.展开更多
We designed boron-doped nanocrystalline diamond microelectrode arrays（BNCD-MEAs） with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H2 O...We designed boron-doped nanocrystalline diamond microelectrode arrays（BNCD-MEAs） with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H2 O2[2_TD$IF]released from stimulated cells by ascorbic acid（AA）. Our observations demonstrated that the as-prepared diamond microelectrode arrays could be utilized to distinguish cancer cells from normal cells, and the amperometric study showed the considerable differences in the currents, indicating that the related Hep G2 cancer cells could release more H2 O2 than that of L02 normal cells. This supports the possibility to use diamond-based MEAs for rapid cancer cell detection in future clinic applications.展开更多
基金funded by the 863 project(2015AA020502)National Natural Science Foundation of China(61401217,61527806)+1 种基金Natural Science Foundation of Jiangsu Province(BK20140900)the Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province[(2013)448]
文摘Biomedical applications of nanomaterials are exponentially increasing every year due to analogy to various cell receptors, ligands, structural proteins, and genetic materials(that is, DNA). In bone tissue, nanoscale materials can provide scaffold for excellent tissue repair via mechanical stimulation, releasing of various loaded drugs and mediators, 3D scaffold for cell growth and differentiation of bone marrow stem cells to osteocytes. This review will therefore highlight recent advancements on tissue and nanoscale materials interaction.
基金This work is supported by the National High-tech R＆D Program of China （No. 2015AA020502） and the National Natural Science Foundation of China （Nos. 81325011, 21327902 and 21175020）. M. S. acknowledges support from the NSF-PREM program （No, DRM-1523588）.
文摘Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging method for accurate and rapid diagnosis of cancer cells by introducing aqueous Fe^2＋ and Zn^2＋ ions into cancer cells （i.e., HeLa, U87, and HepG2 cancer cells）. We found that the biocompatible metal ions Fe^2＋ and Zn^2＋ forced the cancer cells to spontaneously synthesize fluorescent ZnO nanoclusters and magnetic Fe3O4 nanoclusters. These clusters could then be used for multimodal cancer imaging by combining fluorescence imaging with magnetic resonance imaging and computed tomography imaging. Meanwhile, for normal cells （i.e., L02） and tissues, neither fluorescence nor any other obvious difference could be detected between pre- and post-injection. This multimodal bioimaging strategy based on the in situ biosynthesized Zn＆Fe oxide nanoclusters might therefore be useful for early cancer diagnosis and therapy.
基金supported by the National Natural Science Foundation of China (81325011)National High Technology Research & Development Program of China (2015AA020502, 2012AA022703)+1 种基金the Major Science & Technology Project of Suzhou (ZXY2012028)the National Science Foundation CREST program (NSF HRD-0932421)
文摘Photodynamic therapy （PDT） is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficacy, e.g. effective concentra- tion of the drug at the desired sites, the irradiation light dosimetry and biocompatibility of the photosensitizer. The introduction of nanotechnology and nanomaterial like biocompatible nano-titania （i.e., nano-TiO2） may facilitate to solve some of these problems. In this study we have explored the possibility of combining tetra sulphonatophenyl porphyrin （TSPP） with nano-titania （PT） for efficient PDT with least adverse effects. The spectroscopic properties of these nano-cornposites were characterized by using fluorescence and UV-Vis absorption spectroscopic study. The singlet oxygen quantum yield was deter- mined by using 2,5-diphenyl-3,4-benzofuran （DPBF）, while the effect of nano TiO2 with TSPP on the synovial fibroblast cells from human （HSC） and rat models （RSC） were investigated by confocal laser microscopy and 3-（4,5-dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay. Our results suggest that nano TiO2 with TSPP can be readily utilized for effec- tive PDT treatment of Rheumatoid Arthritis （RA）.
基金financially supported by the National High Technology Research and Development Program of China (No. 2015AA020502)the National Natural Science Foundation of China (Nos. 81325011, 21175020, 21327902)+3 种基金the Fundamental Research Funds for the Central Universities of China (No. 2242016K41023)the Project "Strategic Partnerships U5" of Ulm University, Ulm, Germanyfunded by the German Academic Exchange Service (DAAD)the German Ministry for Education and Research (BMBF)
文摘We designed boron-doped nanocrystalline diamond microelectrode arrays（BNCD-MEAs） with 16 channels for the bioanalysis of multicellular samples, which could be readily adapted for a highly sensitive detection of H2 O2[2_TD$IF]released from stimulated cells by ascorbic acid（AA）. Our observations demonstrated that the as-prepared diamond microelectrode arrays could be utilized to distinguish cancer cells from normal cells, and the amperometric study showed the considerable differences in the currents, indicating that the related Hep G2 cancer cells could release more H2 O2 than that of L02 normal cells. This supports the possibility to use diamond-based MEAs for rapid cancer cell detection in future clinic applications.
