AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control...AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.展开更多
Objective: To examine the daily life care, spiritual comfort, and fall safety care needs of the elderly in urban and rural areas of Tangshan, along with their influencing factors. Methods: From August 2022 to April 20...Objective: To examine the daily life care, spiritual comfort, and fall safety care needs of the elderly in urban and rural areas of Tangshan, along with their influencing factors. Methods: From August 2022 to April 2023, an investigation was conducted among urban and rural elderly individuals aged over 75 years in Tangshan City using the Activities of Daily Living Scale, the Revised Community Elderly Fall Risk Assessment Tool, and the Loneliness Scale. Results: The study included 750 urban and 740 rural elderly individuals aged over 75 years. Matrix analysis revealed a significant proportion of fall safety care needs across various daily life and spiritual care requirements. Multiple factor analysis indicated that advanced age, lower education levels, a greater number of chronic diseases, and lower levels of family and social support were associated with higher care demands among the elderly in both urban and rural areas. These differences were statistically significant (P < 0.05). Conclusion: The elderly in urban and rural areas demonstrate a high demand for fall safety care. Particular attention should be given to individuals with lower education levels, those who are widowed, those with multiple chronic diseases, and those with low levels of family and social support to better meet the diverse care needs of this population.展开更多
基金Supported by the National Natural Science Foundation of China(No.81770898).
文摘AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.
基金Model Study on the Care Effectiveness of Intergenerational Care for Disabled Elderly in Rural Areas under the Background of Silver Age(School level Project Y24-08)。
文摘Objective: To examine the daily life care, spiritual comfort, and fall safety care needs of the elderly in urban and rural areas of Tangshan, along with their influencing factors. Methods: From August 2022 to April 2023, an investigation was conducted among urban and rural elderly individuals aged over 75 years in Tangshan City using the Activities of Daily Living Scale, the Revised Community Elderly Fall Risk Assessment Tool, and the Loneliness Scale. Results: The study included 750 urban and 740 rural elderly individuals aged over 75 years. Matrix analysis revealed a significant proportion of fall safety care needs across various daily life and spiritual care requirements. Multiple factor analysis indicated that advanced age, lower education levels, a greater number of chronic diseases, and lower levels of family and social support were associated with higher care demands among the elderly in both urban and rural areas. These differences were statistically significant (P < 0.05). Conclusion: The elderly in urban and rural areas demonstrate a high demand for fall safety care. Particular attention should be given to individuals with lower education levels, those who are widowed, those with multiple chronic diseases, and those with low levels of family and social support to better meet the diverse care needs of this population.
