Objective:To entrap carvacrol(CAR)in bovine serum albumin nanoparticles(BSANPs)to form CAR-loaded BSANPs(CAR@BSANPs)and to explore the anti-cancer effects in breast adenocarcinoma cells(MCF-7 cells)treated with CAR an...Objective:To entrap carvacrol(CAR)in bovine serum albumin nanoparticles(BSANPs)to form CAR-loaded BSANPs(CAR@BSANPs)and to explore the anti-cancer effects in breast adenocarcinoma cells(MCF-7 cells)treated with CAR and CAR@BSANPs.Methods:A desolvation method was used to synthesize BSANPs and CAR@BSANPs.The BSANPs and CAR@BSANPs were characterized by several physicochemical methods,including visual observation,high-resolution field emission scanning electron microscopy,Fourier transform infrared spectroscopy,and high-performance liquid chromatography.MCF-7 cells were used and analyzed after 24 h of exposure to CAR and CAR@BSANPs at half-maximal inhibitory concentration.The anti-proliferative,apoptotic,reactive oxygen species(ROS),and nitric oxide(NO)scavenging activity as well as gene expression analysis were investigated by the cell viability assay,phase-contrast microscopy,2',7'-dichlorofluorescein-diacetate assay,Griess-Illosvoy colorimetric assay,and quantitative real-time polymerase chain reaction,respectively.Results:CAR and CAR@BSANPs showed anti-proliferative,apoptotic,ROS generation,and NO scavenging effects on MCF-7 cells.Expression profile of B-cell lymphoma 2-like 11(BCL2L11),vascular endothelial growth factor A(VEGFA),hypoxia inducible factor factor-1α(HIF1A),BCL2L11/apoptosis regulator(BAX),and BCL2L11/Bcl2 homologous antagonist/killer 1(BAK1)ratios revealed downregulated genes;and BAX,BAK1,and CASP8 were upregulated by CAR and CAR@BSANPs treatment.In vitro anticancer assays of the CAR and CAR@BSANPs showed that CAR@BSANPs demonstrated higher therapeutic efficacy in the MCF-7 cells than CAR.Conclusions:CAR and CAR@BSANPs affect gene expression and may subsequently reduce the growth and proliferation of the MCF-7 cells.Molecular targeting of regulatory genes of the MCF-7 cells with CAR and CAR@BSANPs may be an effective therapeutic strategy against breast cancer.展开更多
文摘Objective:To entrap carvacrol(CAR)in bovine serum albumin nanoparticles(BSANPs)to form CAR-loaded BSANPs(CAR@BSANPs)and to explore the anti-cancer effects in breast adenocarcinoma cells(MCF-7 cells)treated with CAR and CAR@BSANPs.Methods:A desolvation method was used to synthesize BSANPs and CAR@BSANPs.The BSANPs and CAR@BSANPs were characterized by several physicochemical methods,including visual observation,high-resolution field emission scanning electron microscopy,Fourier transform infrared spectroscopy,and high-performance liquid chromatography.MCF-7 cells were used and analyzed after 24 h of exposure to CAR and CAR@BSANPs at half-maximal inhibitory concentration.The anti-proliferative,apoptotic,reactive oxygen species(ROS),and nitric oxide(NO)scavenging activity as well as gene expression analysis were investigated by the cell viability assay,phase-contrast microscopy,2',7'-dichlorofluorescein-diacetate assay,Griess-Illosvoy colorimetric assay,and quantitative real-time polymerase chain reaction,respectively.Results:CAR and CAR@BSANPs showed anti-proliferative,apoptotic,ROS generation,and NO scavenging effects on MCF-7 cells.Expression profile of B-cell lymphoma 2-like 11(BCL2L11),vascular endothelial growth factor A(VEGFA),hypoxia inducible factor factor-1α(HIF1A),BCL2L11/apoptosis regulator(BAX),and BCL2L11/Bcl2 homologous antagonist/killer 1(BAK1)ratios revealed downregulated genes;and BAX,BAK1,and CASP8 were upregulated by CAR and CAR@BSANPs treatment.In vitro anticancer assays of the CAR and CAR@BSANPs showed that CAR@BSANPs demonstrated higher therapeutic efficacy in the MCF-7 cells than CAR.Conclusions:CAR and CAR@BSANPs affect gene expression and may subsequently reduce the growth and proliferation of the MCF-7 cells.Molecular targeting of regulatory genes of the MCF-7 cells with CAR and CAR@BSANPs may be an effective therapeutic strategy against breast cancer.
