The emergence of Web3 technologies promises to revolutionize the Internet and redefine our interactions with digital assets and applications.This essay explores the pivotal role of 5G infrastructure in bolstering the ...The emergence of Web3 technologies promises to revolutionize the Internet and redefine our interactions with digital assets and applications.This essay explores the pivotal role of 5G infrastructure in bolstering the growth and potential of Web3.By focusing on several crucial aspects—network speed,edge computing,network capacity,security and power consumption—we shed light on how 5G technology offers a robust and transformative foundation for the decentralized future of the Internet.Prior to delving into the specifics,we undertake a technical review of the historical progression and development of Internet and telecommunication technologies.展开更多
以鲤肾脏组织RNA为模板,扩增并克隆鲤环指蛋白2(RING Finger Protein 2,RNF2)基因的CDS区全长,分析其组织表达谱。结果显示,鲤RNF2基因的CDS区序列长1011 bp,编码336个氨基酸;该基因与鲫、斑马鱼、金头鲷、海洋青鳉鱼、鳕鱼、非洲爪蟾...以鲤肾脏组织RNA为模板,扩增并克隆鲤环指蛋白2(RING Finger Protein 2,RNF2)基因的CDS区全长,分析其组织表达谱。结果显示,鲤RNF2基因的CDS区序列长1011 bp,编码336个氨基酸;该基因与鲫、斑马鱼、金头鲷、海洋青鳉鱼、鳕鱼、非洲爪蟾、尖尾娇鹟、小鼠、智人的同源性分别为98.2%、93.1%、78.0%、79.2%、79.2%、73.9%、78.1%、72.7%、72.7%;理化性质分析表明,RNF2蛋白分子质量为37.44 kD,理论等电点(pI)为6.28,脂肪系数为74.97%,不稳定指数为36.75,无跨膜结构和信号肽;亚细胞定位预测分析表明,该蛋白定位于细胞核(52.2%)、线粒体(26.1%)、细胞质(17.4%)和过氧化物酶体(4.3%)。蛋白质结构域分析表明该蛋白含RING结构域和RAWUL结构域,且RING结构域与斑马鱼、小鼠和智人完全一致;二级结构和三级结构表明,鲤鱼RNF2蛋白含有α-螺旋(35.12%)、延伸链(8.63%)、β-转角(0.89%)和无规则卷曲(55.36%);泛素化底物预测显示,TP53、IRF4、HIST2H2AC、H3F3B和HIST3H3作为RNF2蛋白的底物具有较高的置信度;实时荧光定量PCR结果显示,鲤RNF2基因在肌肉中表达量最高,其次是头肾和肝脏,在肾脏中表达量最低。这些结果为进一步研究RNF2在鲤鱼抗病毒应答中的作用提供了基础信息。展开更多
基金supported by the ZTE Industry-University-Institute Fund Project under Grant No.IA20221202011.
文摘The emergence of Web3 technologies promises to revolutionize the Internet and redefine our interactions with digital assets and applications.This essay explores the pivotal role of 5G infrastructure in bolstering the growth and potential of Web3.By focusing on several crucial aspects—network speed,edge computing,network capacity,security and power consumption—we shed light on how 5G technology offers a robust and transformative foundation for the decentralized future of the Internet.Prior to delving into the specifics,we undertake a technical review of the historical progression and development of Internet and telecommunication technologies.
