This article explores the development of the Internet of Things(IoT)application technology course in higher vocational colleges under the background of“post,course,competition,certificate.”It first emphasizes the im...This article explores the development of the Internet of Things(IoT)application technology course in higher vocational colleges under the background of“post,course,competition,certificate.”It first emphasizes the importance of IoT talent training and course construction in higher vocational colleges and deeply analyzes the core concept of“post,course,competition,certificate”integration.In view of the problems faced in the course construction of IoT application technology in higher vocational colleges and the practical experience of Tianjin Vocational College of Mechanics and Electricity,the implementation strategy of the course construction of IoT application technology in higher vocational colleges is elaborated in detail based on the integrated concept of“post,course,competition,certificate.”展开更多
The defects from electron transport layer,perovskite layer and their interface would result in carrier nonradiative recombination losses.Poor buried interfacial contact is detrimental to charge extraction and device s...The defects from electron transport layer,perovskite layer and their interface would result in carrier nonradiative recombination losses.Poor buried interfacial contact is detrimental to charge extraction and device stability.Here,we report a bottom-up holistic carrier management strategy induced synergistically by multiple chemical bonds to minimize bulk and interfacial energy losses for high-performance perovskite photovoltaics.4-trifluoromethyl-benzamidine hydrochloride(TBHCl)containing–CF_(3),amidine cation and Cl^(-)is in advance incorporated into SnO_(2)colloid solution to realize bottom-up modification.The synergistic effect of multiple functional groups and multiple-bond-induced chemical interaction are revealed theoretically and experimentally.F and Cl^(-)can passivate oxygen vacancy and/or undercoordinated Sn^(4+)defects by coordinating with Sn^(4+).The F can suppress cation migration and modulate crystallization via hydrogen bond with FA^(+),and can passivate lead defects by coordinating with Pb^(2+).The–NH_(2)–C=NH^(+)_(2)and Cl^(-)can passivate cation and anion vacancy defects through ionic bonds with perovskites,respectively.Through TBHCl modification,the suppression of agglomeration of SnO_(2)nanoparticles,bulk and interfacial defect passivation,and release of tensile strains of perovskite films are demonstrated,which resulted in a PCE enhancement from 21.28%to 23.40%and improved stability.With post-treatment,the efficiency is further improved to 23.63%.展开更多
Objective:The aim of the research was to study whether microRNA-15a(miR-15a) oligonucleotide could inhibit cell growth and enhance cytarabine(Ara-C)-induced apoptosis in Raji cells.Methods:Transfecting miR-15a oligonu...Objective:The aim of the research was to study whether microRNA-15a(miR-15a) oligonucleotide could inhibit cell growth and enhance cytarabine(Ara-C)-induced apoptosis in Raji cells.Methods:Transfecting miR-15a oligonucleotide into Raji cells with LipofectamineTM 2000,and then combined with Ara-C.IC50 value and cell proliferation were detected by CCK8 assay;the expression levels of Bcl-2 mRNA and protein were evaluated by RT-PCR and indirect immuno-fluorescence.The apoptotic cells were observed by Hoechst Dyeing;AnnexinV/PI double dyeing method was used to detect the cell apoptotic rate by Flow Cytometry(FCM).Results:After Raji cells were transfected with miR-15a oligonucleotide for 48 h,Bcl2 protein expression levels obviously decreased,however,there was no difference in Bcl-2 mRNA levels,as compared with the control group and blank group(P < 0.05).CCK8 assay showed that miR-15a oligonucleotide decreased the cell growth at 24,48 and 72 h,moreover,miR-15a oligonucleotides combined with Ara-C obviously decreased the cell growth than miR-15a group,Ara-C group and scrambled oligonucleotides(SODN) + Ara-C group.Meanwhile,miR-15a oligonucleotides combined with Ara-C significantly decreased IC50 of Ara-C(10.41 μg/mL),which were obviously lower than those of Ara-C group(15.43 μg/mL) and SODN plus Ara-C group(14.92 μg/mL).Plenty of apoptotic cells could be seen with Hoechst dyeing.AnnexinV/PI double dying assays by FCM indicated that the cell apoptotic rates in earlier period and late period of miR-15a + Ara-C group were 20.93% and 25.27%,respectively,which were obviously higher than those of miR-15a group,Ara-C group and SODN plus Ara-C group.Conclusion:miR-15a oligonucleotides can inhibit cell growth and enhance Ara-C-induced apoptosis in Raji cells.展开更多
Perovskite solar cells(PSCs) have stood out from many photovoltaic technologies due to their flexibility,cost-effectiveness and high-power conversion efficiency(PCE). Nevertheless, the further development of PSCs is g...Perovskite solar cells(PSCs) have stood out from many photovoltaic technologies due to their flexibility,cost-effectiveness and high-power conversion efficiency(PCE). Nevertheless, the further development of PSCs is greatly hindered by the trap-induced non-radiative recombination losses and poor long-term work stability. In the past decade, the huge advancements have been obtained on suppressing nonradiative recombination and enhancing device durability. Among them, the multisite ligands(MSLs) engineering plays a crucial role in precise control and directional modification of functional layers and interfaces,which contributes to markedly increased PCE and lifetimes of PSCs. In view of this, this review summarizes the advances of MSLs in PSCs. From the perspective of functional groups and chemical interaction,the modulation mechanisms of properties of different functional layers and interfaces and device performance via various MSLs are deeply investigated and revealed. Finally, the prospects for the application and development direction of MSLs in PSCs are legitimately proposed.展开更多
Although ionic liquids(ILs)have been widely employed to heal the defects in perovskite solar cells(PSCs),the corresponding defect passivation mechanisms are not thoroughly understood up to now.Herein,we first reveal a...Although ionic liquids(ILs)have been widely employed to heal the defects in perovskite solar cells(PSCs),the corresponding defect passivation mechanisms are not thoroughly understood up to now.Herein,we first reveal an abnormal buried interface anion defect passivation mechanism depending on cationinduced steric hindrance.The IL molecules containing the same anion([BF4]^(-))and different sizes of imidazolium cations induced by substituent size are used to manipulate buried interface.It was revealed what passivated interfacial defects is mainly anions instead of cations.Theoretical and experimental results demonstrate that the large-sized cations can weaken the ionic bond strength between anions and cations,and facilitate the interaction between anions and SnO2as well as perovskites,which is conducive to interfacial defect passivation and ameliorating interfacial contact.It can be concluded that interfacial chemical interaction strength and defect passivation effect are positively correlated with the size of cations.The discovery breaks conventional thinking that large-sized modification molecules would weaken their chemical interaction with perovskite.Compared with the control device(21.54%),the device based on 1,3-Bis(1-adamantyl)-imidazolium tetrafluoroborate(BAIMBF4)with maximum size cations achieves a significantly enhanced efficiency of 23.61%along with much increased moisture,thermal and light stabilities.展开更多
Single-junction and tandem perovskite solar cells(PSCs)have achieved impressive power conversion efficiencies(PCEs)of 25.7%and 31.3%,respectively,which makes it to be one of next-generation photovoltaic technologies[1...Single-junction and tandem perovskite solar cells(PSCs)have achieved impressive power conversion efficiencies(PCEs)of 25.7%and 31.3%,respectively,which makes it to be one of next-generation photovoltaic technologies[1−9].Inter-face engineering[3,5,10−12],composition engineering[13]and ad-ditive engineering[7,14,15]have made remarkable contribu-tions to efficiency enhancement.Compared with efficiency,the long-term operational stability of PSCs jogs along,which is far from the requirements of commercialization.Currently,almost all regular n-i-p PSCs were accomplished with classic-al organic hole-transport materials(HTMs),i.e.,PTAA[16]and spiro-OMeTAD[2,4,6].However,the highly efficient PSCs with the above organic hole-transport layers(HTL)usually suffer from instability.To facilitate hole transport and extraction,LiTF-SI and tBP are frequently employed to dope organic HTLs but this would sacrifice device stability.The use of these hygro-scopic p-dopants endows the devices with poor moisture sta-bility.展开更多
Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia(B-ALL).Methods Relative m RNA levels of BIM, BCL-6...Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia(B-ALL).Methods Relative m RNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference gene.Results Relative expression of BIM, BCL-6, and c-MYC m RNA in B-ALL patients was significantly lower than in healthy controls(P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL patients and those incomplete remission(CR)(P < 0.05). There were no statistically significant differences in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups(P > 0.05). Spearman's rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL patients.Conclusion Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.展开更多
Background:Temporomandibular joint osteoarthritis(TMJOA)is an end-stage disease that seriously affects the patients’quality of life.Molecular insights in advancing our understanding of TMJOA are the need of the hour....Background:Temporomandibular joint osteoarthritis(TMJOA)is an end-stage disease that seriously affects the patients’quality of life.Molecular insights in advancing our understanding of TMJOA are the need of the hour.Methods:We performed RNA high-throughput sequencing and bioinformatics analysis of differentially expressed(DE)long non-coding RNA(lncRNAs),microRNAs(miRNAs),and messenger RNA(mRNAs)in human synovial TMJOA tissues.Firstly,synovium samples of TMJOA patients and non-TMJOA controls were collected for highthroughput sequencing of lncRNAs,miRNAs,and mRNAs.We then performed biological function analysis of the top 100 mRNAs with more than 2-fold differential expression,and their upstream regulated miRNAs and lncRNAs were predicted separately.Intersections between predicted miRNAs/lncRNAs and differentially expressed miRNAs/lncRNAs were determined,respectively.Regulatory networks among the selected lncRNAs,miRNAs,and mRNAs were constructed.Finally,Western Blotting and reverse transcription-quantitative polymerase chain reaction(RT-qPCR)were used to explore the function of selected lncRNA and mRNAs.Results:Our analysis showed that the only upregulated lncRNAxist,was involved in intracellular protein degradation,cartilage matrix degradation,and osteoclast differentiation through four regulatory axes:miR-1271-5p/ctsb,miR-365a-3p/mmp3,miR-199a-3p/fos,and miR-27b-3p/miR-1271-5p/grb2.RT-qPCR results showed that xist expression increased in both TMJOA synovial tissue and inflammatory-stimulated synovial cells.Inhibition of xist could promote the proliferation of synovial cells upon inflammatory stimulation,and xist could positively regulate the expression of GRB2.Targeting GRB2 could inhibit the expression of matrix degrading enzymes in synovial cells,thereby attenuating the inflammatory environment.Conclusion:Given the current lack of reports of lncRNA regulation in TMJOA synovitis,our results revealed that xist was significantly differentially expressed in human synovial TMJOA and could regulate the expression of GRB2,which may play a role in the progression of TMJOA.展开更多
In the past 10 years,perovskite solar cells(PSCs)have undergone extremely rapid development,with a record certified power conversion efficiency(PCE)of 26.7%,which is very close to the limit efficiency.However,the inhe...In the past 10 years,perovskite solar cells(PSCs)have undergone extremely rapid development,with a record certified power conversion efficiency(PCE)of 26.7%,which is very close to the limit efficiency.However,the inherent instability caused by ion migration impedes the realization of long-term operationally stable PSCs.In this review,the types and mechanisms of ion migration occurring in various functional layers of negative-intrinsic-positive(n-i-p)PSCs are summarized.Additionally,methods of suppressing ion migration are systematically discussed.Finally,the prospects of current challenges and future development directions are proposed to advance the achievement of high-performance regular PSCs with high stability and PCE.展开更多
The deep-level traps at grain boundaries(GBs)and halide ion migration are quite challenging for further enhancement of the stability and efficiency of perovskite solar cells(PSCs)as well as for the elimination of noto...The deep-level traps at grain boundaries(GBs)and halide ion migration are quite challenging for further enhancement of the stability and efficiency of perovskite solar cells(PSCs)as well as for the elimination of notorious hysteresis.Herein,we report a large-sized strongly coordinated organic anion GB anchoring strategy for suppressing ion migration and passivating defects in planar PSCs.The practical implementation of this strategy involves the incorporation of potassium salts containing a large-sized organic counter anion(4-sulfobenzoic acid monopotassium salt,SAMS)into the perovskite precursor.It has been found that anions within SAMS can be firmly anchored at GBs due to the strong coordination interaction between C=O and/or S=O at both ends of bulky anion and undercoordinated Pb^(2+)and/or halide vacancies,along with the hydrogen bond between–OH and formamidinium.SAMS can not only passivate shallowlevel defects but also cause more effective passivation of the deep-level defects.The GB manipulation strategy results in a reduced defect density,an increased carrier lifetime as well as suppressed ion migration,which in turn contributed to enhanced efficiency and stability of PSCs together with a thorough elimination of hysteresis.As a result,the SAMSmodified device with an outstanding fill factor of 0.84 delivers a significant improvement in efficiency(22.7%)in comparison with the control device(20.3%).The unencapsulated modified device demonstrates only little degradation after 1320 h at 60℃.展开更多
Introduction:Gastroenteritis caused by non-O1/non-O139 Vibrio cholerae exhibited an increasing trend in recent years in China.Whole genome sequence(WGS)data could play an important role both in the identification of t...Introduction:Gastroenteritis caused by non-O1/non-O139 Vibrio cholerae exhibited an increasing trend in recent years in China.Whole genome sequence(WGS)data could play an important role both in the identification of the outbreaks and in the determination of the serogroup.Here,we present the employment of WGS data in the investigation of two outbreaks caused by non-O1/non-O139 V.cholerae in Guangdong,China,2020-2021.Methods:We obtained the whole genome sequence of 66 V.cholerae strains isolated in two outbreaks with next generation sequencing technology.We retrieved the publicly available WGS data of non-O1/non-O139 V.cholerae from public database.We used a pipeline integrated in China Pathogen Identification Net(PIN)to complete the phylogenetic analysis.Results:Two outbreaks caused by non-O1/non-O139 V.cholerae were identified using WGS data.These V.cholerae strains were determined as serogroup O5.Type 3 and 6 secretion systems were detected in these serogroup O5 strains.These serogroup O5 strains belonged to sequence type(ST)88.Conclusions:Our analysis indicated the risk of non-O1/non-O139 V.cholerae leading to outbreaks of diarrheal diseases.The application of genomic data played an important role in the identification of the serogroup of non-O1/non-O139 V.cholerae in the lack of antiserum,which gave an example of the application of genome data in disease surveillance and public health emergency response.展开更多
The past decade has witnessed the rapid development of perovskite solar cells,with their power conversion efficiency increasing from an initial 3.8%to over 26%,approaching the Shockley-Queisser(S-Q)limit for single-ju...The past decade has witnessed the rapid development of perovskite solar cells,with their power conversion efficiency increasing from an initial 3.8%to over 26%,approaching the Shockley-Queisser(S-Q)limit for single-junction solar cells.Multijunction solar cells have garnered significant attention due to their tremendous potential to surpass the S-Q limit by reducing thermalization losses and wide light harvesting.The wide bandgap tunability of metal halide perovskite materials makes them highly suitable for sub-cells in tandem solar cells(TSCs).Currently,LONGi Green Energy Technology Co.,Ltd.in China has set a world record efficiency of 34.6% based on a dual-junction perovskitesilicon TSCs,far surpassing the single-junction efficiencies of each sub-cell.Consequently,perovskite based TSCs are widely regarded as the next-generation photovoltaic products in the solar industry.Despite the significant efficiency improvements,several challenges still impede the commercial application of perovskite based TSCs,such as the instability of perovskite materials and difficulties in achieving large-scale production.This review summarizes the progresses and optimization strategies of perovskite based TSCs.This review also identifies the critical issues hindering multijunction solar cells.Finally,the potential solutions to address these challenges are proposed to advance the development of perovskite based TSCs.展开更多
基金2021 Tianjin Educational Science Planning Project“Research and Practice of Talent Cultivation Mode for IoT Majors in Higher Vocational Colleges under the Guidance of the‘1+X’Certificate System”(CJE210237)。
文摘This article explores the development of the Internet of Things(IoT)application technology course in higher vocational colleges under the background of“post,course,competition,certificate.”It first emphasizes the importance of IoT talent training and course construction in higher vocational colleges and deeply analyzes the core concept of“post,course,competition,certificate”integration.In view of the problems faced in the course construction of IoT application technology in higher vocational colleges and the practical experience of Tianjin Vocational College of Mechanics and Electricity,the implementation strategy of the course construction of IoT application technology in higher vocational colleges is elaborated in detail based on the integrated concept of“post,course,competition,certificate.”
基金financially supported by the Support Plan for Overseas Students to Return to China for Entrepreneurship and Innovation(cx2020003)the Fundamental Research Funds for the Central Universities(2020CDJ-LHZZ-074)the Natural Science Foundation of Chongqing(cstc2020jcyj-msxm X0629)。
文摘The defects from electron transport layer,perovskite layer and their interface would result in carrier nonradiative recombination losses.Poor buried interfacial contact is detrimental to charge extraction and device stability.Here,we report a bottom-up holistic carrier management strategy induced synergistically by multiple chemical bonds to minimize bulk and interfacial energy losses for high-performance perovskite photovoltaics.4-trifluoromethyl-benzamidine hydrochloride(TBHCl)containing–CF_(3),amidine cation and Cl^(-)is in advance incorporated into SnO_(2)colloid solution to realize bottom-up modification.The synergistic effect of multiple functional groups and multiple-bond-induced chemical interaction are revealed theoretically and experimentally.F and Cl^(-)can passivate oxygen vacancy and/or undercoordinated Sn^(4+)defects by coordinating with Sn^(4+).The F can suppress cation migration and modulate crystallization via hydrogen bond with FA^(+),and can passivate lead defects by coordinating with Pb^(2+).The–NH_(2)–C=NH^(+)_(2)and Cl^(-)can passivate cation and anion vacancy defects through ionic bonds with perovskites,respectively.Through TBHCl modification,the suppression of agglomeration of SnO_(2)nanoparticles,bulk and interfacial defect passivation,and release of tensile strains of perovskite films are demonstrated,which resulted in a PCE enhancement from 21.28%to 23.40%and improved stability.With post-treatment,the efficiency is further improved to 23.63%.
基金Supported by the grants from the Natural Science Program Foundation of the Guangdong Province (No.04010446)the Overseas Chinese Affairs Office of the State Council Key Discipline Construction Fund (No.51205002)
文摘Objective:The aim of the research was to study whether microRNA-15a(miR-15a) oligonucleotide could inhibit cell growth and enhance cytarabine(Ara-C)-induced apoptosis in Raji cells.Methods:Transfecting miR-15a oligonucleotide into Raji cells with LipofectamineTM 2000,and then combined with Ara-C.IC50 value and cell proliferation were detected by CCK8 assay;the expression levels of Bcl-2 mRNA and protein were evaluated by RT-PCR and indirect immuno-fluorescence.The apoptotic cells were observed by Hoechst Dyeing;AnnexinV/PI double dyeing method was used to detect the cell apoptotic rate by Flow Cytometry(FCM).Results:After Raji cells were transfected with miR-15a oligonucleotide for 48 h,Bcl2 protein expression levels obviously decreased,however,there was no difference in Bcl-2 mRNA levels,as compared with the control group and blank group(P < 0.05).CCK8 assay showed that miR-15a oligonucleotide decreased the cell growth at 24,48 and 72 h,moreover,miR-15a oligonucleotides combined with Ara-C obviously decreased the cell growth than miR-15a group,Ara-C group and scrambled oligonucleotides(SODN) + Ara-C group.Meanwhile,miR-15a oligonucleotides combined with Ara-C significantly decreased IC50 of Ara-C(10.41 μg/mL),which were obviously lower than those of Ara-C group(15.43 μg/mL) and SODN plus Ara-C group(14.92 μg/mL).Plenty of apoptotic cells could be seen with Hoechst dyeing.AnnexinV/PI double dying assays by FCM indicated that the cell apoptotic rates in earlier period and late period of miR-15a + Ara-C group were 20.93% and 25.27%,respectively,which were obviously higher than those of miR-15a group,Ara-C group and SODN plus Ara-C group.Conclusion:miR-15a oligonucleotides can inhibit cell growth and enhance Ara-C-induced apoptosis in Raji cells.
基金financially supported by the National Natural Science Foundation of China (62274018)the Xinjiang Construction Corps Key Areas of Science and Technology Research Project (2023AB029)the Key Project of Chongqing Overseas Students Returning to China Entrepreneurship and Innovation Support Plan (cx2023006)。
文摘Perovskite solar cells(PSCs) have stood out from many photovoltaic technologies due to their flexibility,cost-effectiveness and high-power conversion efficiency(PCE). Nevertheless, the further development of PSCs is greatly hindered by the trap-induced non-radiative recombination losses and poor long-term work stability. In the past decade, the huge advancements have been obtained on suppressing nonradiative recombination and enhancing device durability. Among them, the multisite ligands(MSLs) engineering plays a crucial role in precise control and directional modification of functional layers and interfaces,which contributes to markedly increased PCE and lifetimes of PSCs. In view of this, this review summarizes the advances of MSLs in PSCs. From the perspective of functional groups and chemical interaction,the modulation mechanisms of properties of different functional layers and interfaces and device performance via various MSLs are deeply investigated and revealed. Finally, the prospects for the application and development direction of MSLs in PSCs are legitimately proposed.
基金financially supported by the Support Plan for Overseas Students to Return to China for Entrepreneurship and Innovation(cx2020003)the Fundamental Research Funds for the Central Universities(2020CDJ-LHZZ-074 and 2021CDJQY-022)Natural Science Foundation of Chongqing(cstc2020jcyjmsxmX0629)。
文摘Although ionic liquids(ILs)have been widely employed to heal the defects in perovskite solar cells(PSCs),the corresponding defect passivation mechanisms are not thoroughly understood up to now.Herein,we first reveal an abnormal buried interface anion defect passivation mechanism depending on cationinduced steric hindrance.The IL molecules containing the same anion([BF4]^(-))and different sizes of imidazolium cations induced by substituent size are used to manipulate buried interface.It was revealed what passivated interfacial defects is mainly anions instead of cations.Theoretical and experimental results demonstrate that the large-sized cations can weaken the ionic bond strength between anions and cations,and facilitate the interaction between anions and SnO2as well as perovskites,which is conducive to interfacial defect passivation and ameliorating interfacial contact.It can be concluded that interfacial chemical interaction strength and defect passivation effect are positively correlated with the size of cations.The discovery breaks conventional thinking that large-sized modification molecules would weaken their chemical interaction with perovskite.Compared with the control device(21.54%),the device based on 1,3-Bis(1-adamantyl)-imidazolium tetrafluoroborate(BAIMBF4)with maximum size cations achieves a significantly enhanced efficiency of 23.61%along with much increased moisture,thermal and light stabilities.
基金supported by the National Natural Science Foundation of China(62274018)the Fundamental Research Funds for the Central Universities(2020CDJ-LHZZ-074)+4 种基金the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0629)the Support Plan for Overseas Students to Return to China for Entrepreneurship and Innovation(cx2020003)L.Ding thanks the National Key Research and Development Program of China(2022YFB3803300)the open research fund of Songshan Lake Materials Laboratory(2021SLABFK02)the National Natural Science Foundation of China(21961160720).
文摘Single-junction and tandem perovskite solar cells(PSCs)have achieved impressive power conversion efficiencies(PCEs)of 25.7%and 31.3%,respectively,which makes it to be one of next-generation photovoltaic technologies[1−9].Inter-face engineering[3,5,10−12],composition engineering[13]and ad-ditive engineering[7,14,15]have made remarkable contribu-tions to efficiency enhancement.Compared with efficiency,the long-term operational stability of PSCs jogs along,which is far from the requirements of commercialization.Currently,almost all regular n-i-p PSCs were accomplished with classic-al organic hole-transport materials(HTMs),i.e.,PTAA[16]and spiro-OMeTAD[2,4,6].However,the highly efficient PSCs with the above organic hole-transport layers(HTL)usually suffer from instability.To facilitate hole transport and extraction,LiTF-SI and tBP are frequently employed to dope organic HTLs but this would sacrifice device stability.The use of these hygro-scopic p-dopants endows the devices with poor moisture sta-bility.
基金Supported by the gants of the Guangdong Province Key Foundation of Science and Technology Program(No.2009B0507000029)the Major Research Plan of the National Natural Science Foundation of China(cultivating project,No.91129720)+1 种基金the Guangdong Science&Technology Project(Nos.2012B050600023 and 2015A050502029)a Grant from the Overseas Chinese Affairs Office of the State Council Key Discipline Construction Fund(No.51205002)
文摘Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia(B-ALL).Methods Relative m RNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference gene.Results Relative expression of BIM, BCL-6, and c-MYC m RNA in B-ALL patients was significantly lower than in healthy controls(P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL patients and those incomplete remission(CR)(P < 0.05). There were no statistically significant differences in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups(P > 0.05). Spearman's rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL patients.Conclusion Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.
基金the National Natural Science Foundation of China(32071313,82270996)the Science and Technology Commission of Shanghai Municipality Science Research Project(20S31902500,20Y11903900)the Cross-Disciplinary Research Fund of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202203).
文摘Background:Temporomandibular joint osteoarthritis(TMJOA)is an end-stage disease that seriously affects the patients’quality of life.Molecular insights in advancing our understanding of TMJOA are the need of the hour.Methods:We performed RNA high-throughput sequencing and bioinformatics analysis of differentially expressed(DE)long non-coding RNA(lncRNAs),microRNAs(miRNAs),and messenger RNA(mRNAs)in human synovial TMJOA tissues.Firstly,synovium samples of TMJOA patients and non-TMJOA controls were collected for highthroughput sequencing of lncRNAs,miRNAs,and mRNAs.We then performed biological function analysis of the top 100 mRNAs with more than 2-fold differential expression,and their upstream regulated miRNAs and lncRNAs were predicted separately.Intersections between predicted miRNAs/lncRNAs and differentially expressed miRNAs/lncRNAs were determined,respectively.Regulatory networks among the selected lncRNAs,miRNAs,and mRNAs were constructed.Finally,Western Blotting and reverse transcription-quantitative polymerase chain reaction(RT-qPCR)were used to explore the function of selected lncRNA and mRNAs.Results:Our analysis showed that the only upregulated lncRNAxist,was involved in intracellular protein degradation,cartilage matrix degradation,and osteoclast differentiation through four regulatory axes:miR-1271-5p/ctsb,miR-365a-3p/mmp3,miR-199a-3p/fos,and miR-27b-3p/miR-1271-5p/grb2.RT-qPCR results showed that xist expression increased in both TMJOA synovial tissue and inflammatory-stimulated synovial cells.Inhibition of xist could promote the proliferation of synovial cells upon inflammatory stimulation,and xist could positively regulate the expression of GRB2.Targeting GRB2 could inhibit the expression of matrix degrading enzymes in synovial cells,thereby attenuating the inflammatory environment.Conclusion:Given the current lack of reports of lncRNA regulation in TMJOA synovitis,our results revealed that xist was significantly differentially expressed in human synovial TMJOA and could regulate the expression of GRB2,which may play a role in the progression of TMJOA.
基金financially supported by the National Natural Sci-ence Foundation of China(62274018,52462031)the Xinjiang Construction Corps Key Areas of Science and Technology Research Project(2023AB029)+1 种基金the Tianchi Talent Program of Xinjiang Uygur Autonomous Region(2024,Jiangzhao Chen)the Key Project of Chongqing Overseas Students Returning to China Entrepreneurship and Innovation Support Plan(cx2023006).
文摘In the past 10 years,perovskite solar cells(PSCs)have undergone extremely rapid development,with a record certified power conversion efficiency(PCE)of 26.7%,which is very close to the limit efficiency.However,the inherent instability caused by ion migration impedes the realization of long-term operationally stable PSCs.In this review,the types and mechanisms of ion migration occurring in various functional layers of negative-intrinsic-positive(n-i-p)PSCs are summarized.Additionally,methods of suppressing ion migration are systematically discussed.Finally,the prospects of current challenges and future development directions are proposed to advance the achievement of high-performance regular PSCs with high stability and PCE.
基金the Support Plan for Overseas Students to Return to China for Entrepreneurship and Innovation(cx2020003)the Fundamental Research Funds for the Central Universities(2020CDJQY-A028 and 2020CDJ-LHZZ-074)the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0629)。
文摘The deep-level traps at grain boundaries(GBs)and halide ion migration are quite challenging for further enhancement of the stability and efficiency of perovskite solar cells(PSCs)as well as for the elimination of notorious hysteresis.Herein,we report a large-sized strongly coordinated organic anion GB anchoring strategy for suppressing ion migration and passivating defects in planar PSCs.The practical implementation of this strategy involves the incorporation of potassium salts containing a large-sized organic counter anion(4-sulfobenzoic acid monopotassium salt,SAMS)into the perovskite precursor.It has been found that anions within SAMS can be firmly anchored at GBs due to the strong coordination interaction between C=O and/or S=O at both ends of bulky anion and undercoordinated Pb^(2+)and/or halide vacancies,along with the hydrogen bond between–OH and formamidinium.SAMS can not only passivate shallowlevel defects but also cause more effective passivation of the deep-level defects.The GB manipulation strategy results in a reduced defect density,an increased carrier lifetime as well as suppressed ion migration,which in turn contributed to enhanced efficiency and stability of PSCs together with a thorough elimination of hysteresis.As a result,the SAMSmodified device with an outstanding fill factor of 0.84 delivers a significant improvement in efficiency(22.7%)in comparison with the control device(20.3%).The unencapsulated modified device demonstrates only little degradation after 1320 h at 60℃.
基金Guangdong Collaborative Innovation Center for Prevention and Control of Cross-border Transmission of Infectious Diseases(Project No.2018B020207013).
文摘Introduction:Gastroenteritis caused by non-O1/non-O139 Vibrio cholerae exhibited an increasing trend in recent years in China.Whole genome sequence(WGS)data could play an important role both in the identification of the outbreaks and in the determination of the serogroup.Here,we present the employment of WGS data in the investigation of two outbreaks caused by non-O1/non-O139 V.cholerae in Guangdong,China,2020-2021.Methods:We obtained the whole genome sequence of 66 V.cholerae strains isolated in two outbreaks with next generation sequencing technology.We retrieved the publicly available WGS data of non-O1/non-O139 V.cholerae from public database.We used a pipeline integrated in China Pathogen Identification Net(PIN)to complete the phylogenetic analysis.Results:Two outbreaks caused by non-O1/non-O139 V.cholerae were identified using WGS data.These V.cholerae strains were determined as serogroup O5.Type 3 and 6 secretion systems were detected in these serogroup O5 strains.These serogroup O5 strains belonged to sequence type(ST)88.Conclusions:Our analysis indicated the risk of non-O1/non-O139 V.cholerae leading to outbreaks of diarrheal diseases.The application of genomic data played an important role in the identification of the serogroup of non-O1/non-O139 V.cholerae in the lack of antiserum,which gave an example of the application of genome data in disease surveillance and public health emergency response.
基金supported by the National Natural Science Foundation of China(62274018)the Xinjiang Construction Corps Key Areas of Science and Technology Research Project(2023AB029)the Key Project of Chongqing Overseas Students Returning to China Entrepreneurship and Innovation Support Plan(cx2023006).
文摘The past decade has witnessed the rapid development of perovskite solar cells,with their power conversion efficiency increasing from an initial 3.8%to over 26%,approaching the Shockley-Queisser(S-Q)limit for single-junction solar cells.Multijunction solar cells have garnered significant attention due to their tremendous potential to surpass the S-Q limit by reducing thermalization losses and wide light harvesting.The wide bandgap tunability of metal halide perovskite materials makes them highly suitable for sub-cells in tandem solar cells(TSCs).Currently,LONGi Green Energy Technology Co.,Ltd.in China has set a world record efficiency of 34.6% based on a dual-junction perovskitesilicon TSCs,far surpassing the single-junction efficiencies of each sub-cell.Consequently,perovskite based TSCs are widely regarded as the next-generation photovoltaic products in the solar industry.Despite the significant efficiency improvements,several challenges still impede the commercial application of perovskite based TSCs,such as the instability of perovskite materials and difficulties in achieving large-scale production.This review summarizes the progresses and optimization strategies of perovskite based TSCs.This review also identifies the critical issues hindering multijunction solar cells.Finally,the potential solutions to address these challenges are proposed to advance the development of perovskite based TSCs.
