The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the t...The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.展开更多
Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiame...Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiameter vascular grafts(SDVGs)with on-demand NO and H_(2)S release capabilities,were designed and fabri-cated.The keratin-based H_(2)S donor(KTC)with good biocompatibility and high stability was first synthesized and then electrospun with poly(L-lactide-co-caprolactone)(PLCL)to be used as the outer layer of grafts.The elec-trospun poly(ε-caprolactone)(PCL)mats were aminolyzed and further chelated with copper(II)ions to construct glutathione peroxidase(GPx)-like structural surfaces for the catalytic generation of NO,which acted as the inner layer of grafts.The on-demand release of NO and H_(2)S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells(HUVECs)while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells(HUASMCs).Dual releases of NO and H_(2)S gaso-transmitters could enhance their respective production,resulting in enhanced promotion of HUVECs and inhi-bition of HUASMCs owing to their combined actions.In addition,the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress.In rat abdominal aorta replacement models,the grafts remained patency for 6 months.These grafts were capable of facilitating rapid endothelialization and alleviating neo-intimal hyperplasia without obvious injury,inflammation,or thrombosis.More importantly,the grafts were expected to avoid calcification with the degradation of the grafts.Taken together,these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.展开更多
The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the t...The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.展开更多
Pericardial barrier destruction,inflammatory cell infiltration,and fibrous tissue hyperplasia,trigger adhesions after cardiac surgery.There are few anti-adhesion materials that are both functional and sutureable for p...Pericardial barrier destruction,inflammatory cell infiltration,and fibrous tissue hyperplasia,trigger adhesions after cardiac surgery.There are few anti-adhesion materials that are both functional and sutureable for pericardial reconstruction.Besides,a few studies have reported on the mechanism of preventing pericardial adhesion.Herein,a functional barrier membrane with sutureability was developed via a modified electrospinning method.It was composed of poly(L-lactide-co-caprolactone)(PLCL)nanofibers,poly(vinyl alcohol)(PVA)aerogel,and melatonin,named PPMT.The PPMT had a special microstructure manifested as a staggered arrangement of nanofibers on the surface and a layered macroporous aerogel structure in a cross-section.Besides providing the porosity and hydrophilicity obtained from PVA,the structure also had suitable mechanical properties for stitching due to the addition of PLCL nanofibers.Furthermore,it inhibited the proliferation of fibroblasts by suppressing the activation of Fas and P53,and achieved anti-inflammatory effects by affecting the activity of inflammatory cells and reducing the release of pro-inflammatory factors,such as interleukin 8(IL-8)and tumor necrosis factorα(TNF-α).Finally,in vivo transplantation showed that it up-regulated the expression of matrix metalloproteinase-1(MMP1)and tissue inhibitor of metalloproteinase-1(TIMP1),and down-regulated the expression of Vinculin and transforming growth factorβ(TGF-β)in the myocardium,thereby reducing the formation of adhesions.Collectively,these results demonstrate a great potential of PPMT membrane for practical application to anti-adhesion.展开更多
Gelation kinetics is important in tailoring chemically crosslinked hydrogel-based injectable adhesives for different applications.However,the regulation of gelation rate is usually limited to varying the gel precursor...Gelation kinetics is important in tailoring chemically crosslinked hydrogel-based injectable adhesives for different applications.However,the regulation of gelation rate is usually limited to varying the gel precursor and/or crosslinker concentration,which cannot reach a fine level and inevitably alters the physical properties of hydrogels.Amidation reactions are widely used to synthesize hydrogel adhesives.In this work,we propose a traditional Chinese medicine(Borax)-input strategy to tune the gelation rate of amidation reaction triggered systems.Borax provides an initial basic buffer environment to promote the deprotonation process of amino groups and accelerate this reaction.By using a tissue adhesive model PEG-lysozyme(PEG-LZM),the gelation time can be modulated from seconds to minutes with varying Borax concentrations,while the physical properties remain constant.Moreover,the antibacterial ability can be improved due to the bioactivity of Borax.The hydrogel precursors can be regulated to solidify instantly to close the bleeding wound at emergency.Meanwhile,they can also be customized to match the flowing time in the catheter,thereby facilitating minimally invasive tissue sealing.Because this method is easily operated,we envision Borax adjusted amidation-type hydrogel has a promising prospect in clinical application.展开更多
文摘The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.
基金supported by the National Natural Science Fund of China(81873923)Jiangsu Higher Education Institutions(19KJA310001 and PAPD)Jiangsu Collaborative Innovation Center of Biomedical Functional Materials.
文摘Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiameter vascular grafts(SDVGs)with on-demand NO and H_(2)S release capabilities,were designed and fabri-cated.The keratin-based H_(2)S donor(KTC)with good biocompatibility and high stability was first synthesized and then electrospun with poly(L-lactide-co-caprolactone)(PLCL)to be used as the outer layer of grafts.The elec-trospun poly(ε-caprolactone)(PCL)mats were aminolyzed and further chelated with copper(II)ions to construct glutathione peroxidase(GPx)-like structural surfaces for the catalytic generation of NO,which acted as the inner layer of grafts.The on-demand release of NO and H_(2)S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells(HUVECs)while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells(HUASMCs).Dual releases of NO and H_(2)S gaso-transmitters could enhance their respective production,resulting in enhanced promotion of HUVECs and inhi-bition of HUASMCs owing to their combined actions.In addition,the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress.In rat abdominal aorta replacement models,the grafts remained patency for 6 months.These grafts were capable of facilitating rapid endothelialization and alleviating neo-intimal hyperplasia without obvious injury,inflammation,or thrombosis.More importantly,the grafts were expected to avoid calcification with the degradation of the grafts.Taken together,these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.
文摘The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.
基金The National Natural Science Fund of China(81873923)Shanghai Science and Technology Development Fund(20Y11910600,18441901900)supported this study.
文摘Pericardial barrier destruction,inflammatory cell infiltration,and fibrous tissue hyperplasia,trigger adhesions after cardiac surgery.There are few anti-adhesion materials that are both functional and sutureable for pericardial reconstruction.Besides,a few studies have reported on the mechanism of preventing pericardial adhesion.Herein,a functional barrier membrane with sutureability was developed via a modified electrospinning method.It was composed of poly(L-lactide-co-caprolactone)(PLCL)nanofibers,poly(vinyl alcohol)(PVA)aerogel,and melatonin,named PPMT.The PPMT had a special microstructure manifested as a staggered arrangement of nanofibers on the surface and a layered macroporous aerogel structure in a cross-section.Besides providing the porosity and hydrophilicity obtained from PVA,the structure also had suitable mechanical properties for stitching due to the addition of PLCL nanofibers.Furthermore,it inhibited the proliferation of fibroblasts by suppressing the activation of Fas and P53,and achieved anti-inflammatory effects by affecting the activity of inflammatory cells and reducing the release of pro-inflammatory factors,such as interleukin 8(IL-8)and tumor necrosis factorα(TNF-α).Finally,in vivo transplantation showed that it up-regulated the expression of matrix metalloproteinase-1(MMP1)and tissue inhibitor of metalloproteinase-1(TIMP1),and down-regulated the expression of Vinculin and transforming growth factorβ(TGF-β)in the myocardium,thereby reducing the formation of adhesions.Collectively,these results demonstrate a great potential of PPMT membrane for practical application to anti-adhesion.
基金supported by National Natural Science Foundation of China(grant number:31922041,51621002,11932012 , 21875066)the science and technology innovation project of Shanghai Science and Technique Committee(18441908300)the Fundamental Research Funds for the Central Universities(SLD 00202006,JKVD12001032,JKVD12001002,50321041916015).
文摘Gelation kinetics is important in tailoring chemically crosslinked hydrogel-based injectable adhesives for different applications.However,the regulation of gelation rate is usually limited to varying the gel precursor and/or crosslinker concentration,which cannot reach a fine level and inevitably alters the physical properties of hydrogels.Amidation reactions are widely used to synthesize hydrogel adhesives.In this work,we propose a traditional Chinese medicine(Borax)-input strategy to tune the gelation rate of amidation reaction triggered systems.Borax provides an initial basic buffer environment to promote the deprotonation process of amino groups and accelerate this reaction.By using a tissue adhesive model PEG-lysozyme(PEG-LZM),the gelation time can be modulated from seconds to minutes with varying Borax concentrations,while the physical properties remain constant.Moreover,the antibacterial ability can be improved due to the bioactivity of Borax.The hydrogel precursors can be regulated to solidify instantly to close the bleeding wound at emergency.Meanwhile,they can also be customized to match the flowing time in the catheter,thereby facilitating minimally invasive tissue sealing.Because this method is easily operated,we envision Borax adjusted amidation-type hydrogel has a promising prospect in clinical application.
