In this paper,the concept of reversed-or normal-phase chiral stationary phase liquid chromatography has been put forward according to the polar strength of mobile and stationary phases. The statistical model developed...In this paper,the concept of reversed-or normal-phase chiral stationary phase liquid chromatography has been put forward according to the polar strength of mobile and stationary phases. The statistical model developed in HPLC has been used to investigate the separation mechanism of D-and L-enantiomer in chiral stationary phase liquid chromatography.It has been observed that the variation of capacity factor of enantiomers with mobile phase composition in both reversed-phase and normal-phase chiral stationary phase liquid chromatography can be described by the fundamental elution equation lnk'=a+blnC_b+cC_b.The effect of mobile phase composition on the selec- tivity of enantiomers D and L in normal-phase chiral stationary phase liquid chromatography cam be described by the equation lnα=⊿a+⊿blnC_b,but in reversed-phase chiral stationary phase liquid chromatography the selectivity is almost independant of the mobile phase composition.展开更多
The prediction of the peak width at half height is an important aspect in the optimization of the chromatographic operating conditions.In this paper,a linear relationship,between the peak widths at half height and the...The prediction of the peak width at half height is an important aspect in the optimization of the chromatographic operating conditions.In this paper,a linear relationship,between the peak widths at half height and the retention values with various isocratic elution is observed.In gradient elution,however,the relationship between the peak widths at half height and the so-called invented retention values that correspond to the mobile phase composition by eluting the solute from the column end is developed.We believe that there is almost the same band width at half height inside the column (in unit of length)for different solutes.The peak width at half height in the chromatogram(in unit of time)is mainly determined by the capacity factor of the solute when it is eluted front the column end.The larger the capacity factor of a solute eluted from the column end,the more slowly will be the solute eluted from the column end and the wider will be the peak width at half height.It is possible to predict the peak width at half height in various isocratic and gradient elutions by using this linear relationship.展开更多
基金This work was supported by the National Natural Science Foundation of China.
文摘In this paper,the concept of reversed-or normal-phase chiral stationary phase liquid chromatography has been put forward according to the polar strength of mobile and stationary phases. The statistical model developed in HPLC has been used to investigate the separation mechanism of D-and L-enantiomer in chiral stationary phase liquid chromatography.It has been observed that the variation of capacity factor of enantiomers with mobile phase composition in both reversed-phase and normal-phase chiral stationary phase liquid chromatography can be described by the fundamental elution equation lnk'=a+blnC_b+cC_b.The effect of mobile phase composition on the selec- tivity of enantiomers D and L in normal-phase chiral stationary phase liquid chromatography cam be described by the equation lnα=⊿a+⊿blnC_b,but in reversed-phase chiral stationary phase liquid chromatography the selectivity is almost independant of the mobile phase composition.
基金This work was supported by the Foundation of Chinese Academy of Sciences.
文摘The prediction of the peak width at half height is an important aspect in the optimization of the chromatographic operating conditions.In this paper,a linear relationship,between the peak widths at half height and the retention values with various isocratic elution is observed.In gradient elution,however,the relationship between the peak widths at half height and the so-called invented retention values that correspond to the mobile phase composition by eluting the solute from the column end is developed.We believe that there is almost the same band width at half height inside the column (in unit of length)for different solutes.The peak width at half height in the chromatogram(in unit of time)is mainly determined by the capacity factor of the solute when it is eluted front the column end.The larger the capacity factor of a solute eluted from the column end,the more slowly will be the solute eluted from the column end and the wider will be the peak width at half height.It is possible to predict the peak width at half height in various isocratic and gradient elutions by using this linear relationship.
