BACKGROUND： Studies have shown that vascular endothelial growth factor （VEGF） gene polymorphisms highly correlate with sporadic amyotrophic lateral sclerosis （ALS）, although this remains controversial. To date, t...BACKGROUND： Studies have shown that vascular endothelial growth factor （VEGF） gene polymorphisms highly correlate with sporadic amyotrophic lateral sclerosis （ALS）, although this remains controversial. To date, the relationship between VEGF gene polymorphism and sporadic ALS in a Han Chinese population remains unclear. OBJECTIVE： To explore the relationship between sporadic ALS and VEGF gene promoter 7 locus polymorphisms in a Hart Chinese population, and to investigate whether this relationship exhibits gender differences. DESIGN, TIME AND SETTING： A case-control study regarding genetic association was performed at the Central Laboratory of Peking University Third Hospital from 2002 to 2006. PARTICIPANTS： A total of 93 patients, who were diagnosed with definite or probable ALS according to E1 Escorial-revised diagnostic criteria, were selected from the Outpatient Department of Neurology, Peking University Third Hospital from 2002 to 2006. All patients were from Hart populations, with no family history of ALS. In addition, 103 gender- and age-matched healthy volunteers were selected as controls. METHODS： Peripheral venous blood was collected, and whole blood genomic DNA was extracted. VEGF gene promoter 7 locus polymorphisms were analyzed by PCR using the fluorescent Taqman system. The relationship between VEGF gene promoter single nucleotide polymorphisms and ALS was analyzed using Logistic regression model analysis and was stratified according to gender. MAIN OUTCOME MEASURES： 7 VEGF gene promoter polymorphisms genotype distribution and allele frequencies. RESULTS： There were no significant differences in VEGF gene promoter 7 locus genotype distribution and allele frequency between case and control groups （P 〉 0.05）. Stratified analysis based on gender demonstrated that female Han subjects carrying the VEGF genotype -1154AA, -2549TT, -634CC genotype were more susceptible to ALS than those carrying -1154GG, -2549CC, -634GG （VEGF-1154AA： OR= 8.9, 95%CI= 1 .0-77.3, P= 0.047; VEGF-2549TT： OR= 3.1,95% CI= 1.0-9.6, P= 0.049, VEGF-634CC： OR = 0.2, 95%CI= 0.1-0.7, P= 0.012）. Moreover, in female Han populations, people carrying allele -1154A, -2549T, -634C exhibited an increased risk of ALS （VEGF -1154A： OR = 2.3, 95%CI= 1.2 4.5, P= 0.018; VEGF-2549T： OR= 3.1,95%CI= 1.4 6.9, P= 0.005; VEGF-634C： OR= 0.5, 95%CI= 0.3 0.9, P= 0.015）. CONCLUSION： Results showed that VEGF 7 gene promoter polymorphisms were associated with ALS in Han Chinese women. The VEGF gene -1154A, -2549T, -634C allele and -1154AA, -2549TT, -634CC genotype could function as susceptibility genes for ALS in Han Chinese women.展开更多
1|BACKGROUND Noncommunicable diseases(NCDs)are a significant public concern,greatly impacting the economic and social development in China.In 2019,NCDs accounted for a staggering 88.5%of total deaths in China,with car...1|BACKGROUND Noncommunicable diseases(NCDs)are a significant public concern,greatly impacting the economic and social development in China.In 2019,NCDs accounted for a staggering 88.5%of total deaths in China,with cardiovas-cular diseases,cancer,chronic respiratory diseases,and diabetes—the four major chronic diseases—contributing to a premature mortality rate of 16.5%[1].The complexity of NCDs arises from the involvement of multiple genetic and environmental factors that interact in intricate ways.The complexity is characterized by a multitude of interactions among genes,proteins,and metabolic path-ways throughout the various stages of life.展开更多
Dear Editor, Nicotine is a psychoactive alkaloid that is thought to play a key role in addiction to commercial tobacco products [1] and cotinine is its primary metabolite [2]. Pharmacological treatment, such as nicoti...Dear Editor, Nicotine is a psychoactive alkaloid that is thought to play a key role in addiction to commercial tobacco products [1] and cotinine is its primary metabolite [2]. Pharmacological treatment, such as nicotine replacement therapy （NRT）, is a valid solution to this problem. Tobacco smoke contains many carcinogens such as nitrosamines .展开更多
文摘BACKGROUND： Studies have shown that vascular endothelial growth factor （VEGF） gene polymorphisms highly correlate with sporadic amyotrophic lateral sclerosis （ALS）, although this remains controversial. To date, the relationship between VEGF gene polymorphism and sporadic ALS in a Han Chinese population remains unclear. OBJECTIVE： To explore the relationship between sporadic ALS and VEGF gene promoter 7 locus polymorphisms in a Hart Chinese population, and to investigate whether this relationship exhibits gender differences. DESIGN, TIME AND SETTING： A case-control study regarding genetic association was performed at the Central Laboratory of Peking University Third Hospital from 2002 to 2006. PARTICIPANTS： A total of 93 patients, who were diagnosed with definite or probable ALS according to E1 Escorial-revised diagnostic criteria, were selected from the Outpatient Department of Neurology, Peking University Third Hospital from 2002 to 2006. All patients were from Hart populations, with no family history of ALS. In addition, 103 gender- and age-matched healthy volunteers were selected as controls. METHODS： Peripheral venous blood was collected, and whole blood genomic DNA was extracted. VEGF gene promoter 7 locus polymorphisms were analyzed by PCR using the fluorescent Taqman system. The relationship between VEGF gene promoter single nucleotide polymorphisms and ALS was analyzed using Logistic regression model analysis and was stratified according to gender. MAIN OUTCOME MEASURES： 7 VEGF gene promoter polymorphisms genotype distribution and allele frequencies. RESULTS： There were no significant differences in VEGF gene promoter 7 locus genotype distribution and allele frequency between case and control groups （P 〉 0.05）. Stratified analysis based on gender demonstrated that female Han subjects carrying the VEGF genotype -1154AA, -2549TT, -634CC genotype were more susceptible to ALS than those carrying -1154GG, -2549CC, -634GG （VEGF-1154AA： OR= 8.9, 95%CI= 1 .0-77.3, P= 0.047; VEGF-2549TT： OR= 3.1,95% CI= 1.0-9.6, P= 0.049, VEGF-634CC： OR = 0.2, 95%CI= 0.1-0.7, P= 0.012）. Moreover, in female Han populations, people carrying allele -1154A, -2549T, -634C exhibited an increased risk of ALS （VEGF -1154A： OR = 2.3, 95%CI= 1.2 4.5, P= 0.018; VEGF-2549T： OR= 3.1,95%CI= 1.4 6.9, P= 0.005; VEGF-634C： OR= 0.5, 95%CI= 0.3 0.9, P= 0.015）. CONCLUSION： Results showed that VEGF 7 gene promoter polymorphisms were associated with ALS in Han Chinese women. The VEGF gene -1154A, -2549T, -634C allele and -1154AA, -2549TT, -634CC genotype could function as susceptibility genes for ALS in Han Chinese women.
基金National Natural Science Foundation of China,Grant/Award Numbers:81872692,82073642。
文摘1|BACKGROUND Noncommunicable diseases(NCDs)are a significant public concern,greatly impacting the economic and social development in China.In 2019,NCDs accounted for a staggering 88.5%of total deaths in China,with cardiovas-cular diseases,cancer,chronic respiratory diseases,and diabetes—the four major chronic diseases—contributing to a premature mortality rate of 16.5%[1].The complexity of NCDs arises from the involvement of multiple genetic and environmental factors that interact in intricate ways.The complexity is characterized by a multitude of interactions among genes,proteins,and metabolic path-ways throughout the various stages of life.
基金partly supported by the National Natural Science Foundation of China(81271475 and 81571297)
文摘Dear Editor, Nicotine is a psychoactive alkaloid that is thought to play a key role in addiction to commercial tobacco products [1] and cotinine is its primary metabolite [2]. Pharmacological treatment, such as nicotine replacement therapy （NRT）, is a valid solution to this problem. Tobacco smoke contains many carcinogens such as nitrosamines .
