Traditional optimal scheduling methods are limited to accurate physical models and parameter settings, which aredifficult to adapt to the uncertainty of source and load, and there are problems such as the inability to...Traditional optimal scheduling methods are limited to accurate physical models and parameter settings, which aredifficult to adapt to the uncertainty of source and load, and there are problems such as the inability to make dynamicdecisions continuously. This paper proposed a dynamic economic scheduling method for distribution networksbased on deep reinforcement learning. Firstly, the economic scheduling model of the new energy distributionnetwork is established considering the action characteristics of micro-gas turbines, and the dynamic schedulingmodel based on deep reinforcement learning is constructed for the new energy distribution network system with ahigh proportion of new energy, and the Markov decision process of the model is defined. Secondly, Second, for thechanging characteristics of source-load uncertainty, agents are trained interactively with the distributed networkin a data-driven manner. Then, through the proximal policy optimization algorithm, agents adaptively learn thescheduling strategy and realize the dynamic scheduling decision of the new energy distribution network system.Finally, the feasibility and superiority of the proposed method are verified by an improved IEEE 33-node simulationsystem.展开更多
Genotyping arrays based on single nucleotide polymorphisms(SNPs)provide a low-cost,highthroughput platform.The development of a SNP array that fully reflects the genetic diversity of maize(Zea mays L.)germplasm and is...Genotyping arrays based on single nucleotide polymorphisms(SNPs)provide a low-cost,highthroughput platform.The development of a SNP array that fully reflects the genetic diversity of maize(Zea mays L.)germplasm and is applicable to molecular breeding programs is desirable.In this study,we developed a MaizeGerm50K array comprising 50,852 SNPs selected from the resequencing data of 1604 maize inbred lines and other markers.A genome-wide association study using a landrace panel genotyped with the array permitted mapping of several known genes.We also verified a candidate gene,RNA-binding motif protein 24-like 1(ZmRBM24L1),delaying flowering through overexpression lines.Genomic selection for yield and agronomic traits showed high prediction accuracy.The MaizeGerm50K array is thus a valuable genomic tool for maize genetic studies and breeding.展开更多
Previous studies have shown that deep learning is very effective in detecting known attacks.However,when facing unknown attacks,models such as Deep Neural Networks(DNN)combined with Long Short-Term Memory(LSTM),Convol...Previous studies have shown that deep learning is very effective in detecting known attacks.However,when facing unknown attacks,models such as Deep Neural Networks(DNN)combined with Long Short-Term Memory(LSTM),Convolutional Neural Networks(CNN)combined with LSTM,and so on are built by simple stacking,which has the problems of feature loss,low efficiency,and low accuracy.Therefore,this paper proposes an autonomous detectionmodel for Distributed Denial of Service attacks,Multi-Scale Convolutional Neural Network-Bidirectional Gated Recurrent Units-Single Headed Attention(MSCNN-BiGRU-SHA),which is based on a Multistrategy Integrated Zebra Optimization Algorithm(MI-ZOA).The model undergoes training and testing with the CICDDoS2019 dataset,and its performance is evaluated on a new GINKS2023 dataset.The hyperparameters for Conv_filter and GRU_unit are optimized using the Multi-strategy Integrated Zebra Optimization Algorithm(MIZOA).The experimental results show that the test accuracy of the MSCNN-BiGRU-SHA model based on the MIZOA proposed in this paper is as high as 0.9971 in the CICDDoS 2019 dataset.The evaluation accuracy of the new dataset GINKS2023 created in this paper is 0.9386.Compared to the MSCNN-BiGRU-SHA model based on the Zebra Optimization Algorithm(ZOA),the detection accuracy on the GINKS2023 dataset has improved by 5.81%,precisionhas increasedby 1.35%,the recallhas improvedby 9%,and theF1scorehas increasedby 5.55%.Compared to the MSCNN-BiGRU-SHA models developed using Grid Search,Random Search,and Bayesian Optimization,the MSCNN-BiGRU-SHA model optimized with the MI-ZOA exhibits better performance in terms of accuracy,precision,recall,and F1 score.展开更多
Genic male sterility(GMS) is one of the most important resources for exploiting heterosis in crop breeding, so that identifying genomic loci regulating GMS is desirable. However, many regulatory genes controlling GMS ...Genic male sterility(GMS) is one of the most important resources for exploiting heterosis in crop breeding, so that identifying genomic loci regulating GMS is desirable. However, many regulatory genes controlling GMS have not yet been characterized in maize, owing partly to a lack of genetic materials. We generated a recessive male-sterile maize mutant in the Jing 724 genetic background via ethyl methanesulfonate treatment, and found the male sterility to be due to a single gene mutation. Bulk-segregant RNA sequencing of three replicates indicated that one genomic region located at the end of chromosome 4 was associated with the observed mutant phenotype. Among genes with nonsynonymous mutations,Zm00001 d053895(bHLH51) showed abolished expression in the sterile bulks and was annotated as a bHLH transcription factor orthologous to Arabidopsis AMS, suggesting an association with the male sterility of the mutant. Kompetitive Allele-Specific PCR assays further validated the exclusive correlation of male sterility with the single C-to-T mutation in the fifth exon. The new maize mutant and the potential SNP locus provide novel genetic material for investigating the molecular mechanism underlying tapetal development and may facilitate the improvement of hybrid production systems.展开更多
Titanium alloy has been applied in the field of aerospace manufacturing for its high specific strength and hardness.Nonetheless,these properties also cause general problems in the machining,such as processing ineffici...Titanium alloy has been applied in the field of aerospace manufacturing for its high specific strength and hardness.Nonetheless,these properties also cause general problems in the machining,such as processing inefficiency,serious wear,poor workpiece face quality,etc.Aiming at the above problems,this paper carried out a comparative experimental study on titanium alloy milling based on the CAMCand BEMC.The variation law of cutting force and wear morphology of the two tools were obtained,and the wear mechanism and the effect of wear on machining quality were analyzed.The conclusion is that in contrast with BEMC,under the action of cutting thickness thinning mechanism,the force of CAMC was less,and its fluctuation was more stable.The flank wear was uniform and near the cutting edge,and the wear rate was slower.In the early period,the wear mechanism of CAMC was mainly adhesion.Gradually,oxidative wear also occurred with milling.Furthermore,the surface residual height of CAMC was lower.There is no obvious peak and trough accompanied by fewer surface defects.展开更多
Purpose:This study aimed to develop quality indicators for the care of older adults with disabilities in long-term care facilities(LTCFs)based on Maslow’s hierarchy of needs.Methods:The draft of the quality indicator...Purpose:This study aimed to develop quality indicators for the care of older adults with disabilities in long-term care facilities(LTCFs)based on Maslow’s hierarchy of needs.Methods:The draft of the quality indicators was drawn up based on a literature review and research group discussion.The quality indicators were finalized by two rounds of expert consultation(involving 15 experts)using the Delphi method.The Analytic Hierarchy Process was applied to calculate the indicators’weight.Results:The response rates of the two rounds of consultation were 100%and 93%,and the expert authority coefficients were 0.86 and 0.87.After two rounds of consultation,the expert opinion coordination coefficients of the first-,second-and third-level indicators were 0.42,0.25,and 0.96,respectively(P<0.05),and the variation coefficient was0.25.The final quality indicators for the care of older adults with disabilities in LTCFs included 7 first-level,19 second-level,and 107 third-level indicators.Conclusion:The quality indicators for the care of older adults with disabilities in LTCFs are reliable,scientific,comprehensive,and practical and specify the content of person-centered care needs.This can provide a reference for evaluating and improving care quality in LTCFs.展开更多
Swirling addition to the stream is beneficial for the fluid mixing.This work aims to study the mixing process intensification in a conventional T-jets mixer by the swirling addition.After experimental verification by ...Swirling addition to the stream is beneficial for the fluid mixing.This work aims to study the mixing process intensification in a conventional T-jets mixer by the swirling addition.After experimental verification by the planar laser-induced fluorescence technique,large eddy simulation with the dynamic kinetic energy sub-grid stress model is used to predict how the swirling strength(in terms of swirling number,S_(w))and swirling directions affect the mixing performance,e.g.the tracer concentration distribution,mixing time,and turbulent characteristics in the T-jets mixers,Predictions show that the swirling strength is the key factor affecting the mixing efficiency of the process.The overall mixing time,τ_(90),can be significantly reduced by increasing S_(w).Vortex analysis shows that more turbulent eddies appear in the collision zone and the turbulent kinetic energy dissipation rate increases obviously with the swirling addition.When S_(w) is kept constant,the mixing process can be accelerated and intensified by adding swirling to only one stream,to both streams with the opposite swirling directions,or to both streams with the same swirling directions.Amplification of the mixing process by enlarging the mixer size or increasing the flow rates is also optimized.Thus,this work provides a new strategy to improve the mixing performance of the traditional T-jets mixers by the swirling addition.展开更多
[ Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patu/a L. [ Method] By using tissue culture tech- nology, different mass fractions of 6-BA and NAA were added to MS...[ Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patu/a L. [ Method] By using tissue culture tech- nology, different mass fractions of 6-BA and NAA were added to MS medium to compare the effect of different culture medium on the rapid propagation of T. patu/a L. [Result] Shoot tips or stem segments of T. patu/a L. were used as explants for tissue culture with an appropriate sterilization time of 8 min; differentiation effect of shoot tips was better than that of stem segments; callus generation rate was high with the high content of growth regulators; MS medium containing O. 1 mg/L NAA and 1.5 rag/L 6-BA was used for subculture proliferation with a subculture period of 4 weeks; rooting rate of plantlet was the maximum （97%） in 1/2MS medium containing 0.2 mg/L NAA, and the root system was relatively developed. [ Conclusion] This study provided technical support for the industrialized seedling breeding of T. patula L.展开更多
In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and ...In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog （MS） medium supplemented with 0.5 mg/L N-phenyl-N＇-1,2,3-thiadiazol-5-ylurea （TDZ）, 1.0 mg/L 2,4-diehloro- phenoxyacetic acid （2,4-D）, and 30 g/L sucrose （ pH5. 8） in the dark at 25 ±1℃. Further study of the influence of kanamyein and hygromycin on callus induction and multiplication showed that, the lethal doses of kanamycin and hygromycin to bulb explants were 100 and 30 mg/L, respectively. All explants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage, and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg,/L hygromyein, and all the calli died at last. These results will provide important reference for further studies of trausgenic H. vittaturn.展开更多
Objective:To investigate the role of KIF3C gene in promoting the malignant phenotype of lung cancer cells and in regulating PI3K/AKT signaling pathway.Methods:CCK-8 and transwell assays were used to detect the changes...Objective:To investigate the role of KIF3C gene in promoting the malignant phenotype of lung cancer cells and in regulating PI3K/AKT signaling pathway.Methods:CCK-8 and transwell assays were used to detect the changes in cell proliferation and cell migration ability after being transfected with siKIF3C,as well as the protein expression levels of PI3K,p-PI3K,AKT,and p-AKT following the downregulation of KIF3C by Western blot.Results:The CCK-8 assay showed that the proliferation/viability of lung cancer cells A549 significantly reduced after being transfected with siKIF3C gene(P<0.05);the migration ability of lung cancer cells A549 was significantly reduced after transfected with siKIF3C gene(P<0.05);the levels of p-PI3K and p-AKT proteins were downregulated after KIF3C protein knockdown(P<0.05);however,the detection of PI3K and AKT protein levels was not statistically significant.Conclusion:KIF3C may promote the proliferation and migration ability of lung cancer cells A549 through PI3K/AKT signaling pathway.展开更多
The objective of this study is to reveal the role of KIF3C gene in the proliferation of lung cancer cells,and the regulation of epithelial mesenchymal transition(EMT)of tumor cells.The plate clone formation assay and ...The objective of this study is to reveal the role of KIF3C gene in the proliferation of lung cancer cells,and the regulation of epithelial mesenchymal transition(EMT)of tumor cells.The plate clone formation assay and cell scratch assay were used in this study to detect the changes of cell proliferation and migration ability after siKIF3C interference,while EMT-related protein expression after KIF3C downregulation was detected by Western blot.The cell clone formation assay showed that the number of clones of lung cancer cells A549 was significantly reduced after transfected with siKIF3C(P<0.05);The scratch assay showed that the healing ability of cells was significantly reduced after transfected with siKIF3C(P<0.05);Western blot protein analysis revealed that the levels of EMT-related proteins,N-cadherin,Vimentin,Snail,and Slug were significantly down-regulated(P<0.05),however,E-cadherin protein levels were up-regulated after siKIF3C interference.In conclusion,KIF3C may promote the proliferation and invasive ability of lung cancer cells A549 through EMT.展开更多
In the context of enterprise systems,intrusion detection(ID)emerges as a critical element driving the digital transformation of enterprises.With systems spanning various sectors of enterprises geographically dispersed...In the context of enterprise systems,intrusion detection(ID)emerges as a critical element driving the digital transformation of enterprises.With systems spanning various sectors of enterprises geographically dispersed,the necessity for seamless information exchange has surged significantly.The existing cross-domain solutions are challenged by such issues as insufficient security,high communication overhead,and a lack of effective update mechanisms,rendering them less feasible for prolonged application on resource-limited devices.This study proposes a new cross-domain collaboration scheme based on federated chains to streamline the server-side workload.Within this framework,individual nodes solely engage in training local data and subsequently amalgamate the final model employing a federated learning algorithm to uphold enterprise systems with efficiency and security.To curtail the resource utilization of blockchains and deter malicious nodes,a node administration module predicated on the workload paradigm is introduced,enabling the release of surplus resources in response to variations in a node’s contribution metric.Upon encountering an intrusion,the system triggers an alert and logs the characteristics of the breach,facilitating a comprehensive global update across all nodes for collective defense.Experimental results across multiple scenarios have verified the security and effectiveness of the proposed solution,with no loss of its recognition accuracy.展开更多
Small interfering RNA(siRNA)has a promising future in the treatment of ocular diseases due to its high efficiency,specificity,and low toxicity in inhibiting the expression of target genes and proteins.However,due to t...Small interfering RNA(siRNA)has a promising future in the treatment of ocular diseases due to its high efficiency,specificity,and low toxicity in inhibiting the expression of target genes and proteins.However,due to the unique anatomical structure of the eye and various barriers,delivering nucleic acids to the retina remains a significant challenge.In this study,we rationally design PACD,an A-B-C type non-viral vector copolymer composed of a hydrophilic PEG block(A),a siRNA binding block(B)and a pH-responsive block(C).PACDs can self-assemble into nanosized polymeric micelles that compact siRNAs into polyplexes through simple mixing.By evaluating its pH-responsive activity,gene silencing efficiency in retinal cells,intraocular distribution,and anti-angiogenesis therapy in a mouse model of hypoxia-induced angiogenesis,we demonstrate the efficiency and safety of PACD in delivering siRNA in the retina.We are surprised to discover that,the PACD/siRNA polyplexes exhibit remarkable intracellular endosomal escape efficiency,excellent gene silencing,and inhibit retinal angiogenesis.Our study provides design guidance for developing efficient nonviral ocular nucleic acid delivery systems.展开更多
The monkeypox virus(MPXV)outbreak,declared a Public Health Emergency of International Concern(PHEIC)by the World Health Organization(WHO)in 2022,continues to pose a significant threat due to the absence of vaccines or...The monkeypox virus(MPXV)outbreak,declared a Public Health Emergency of International Concern(PHEIC)by the World Health Organization(WHO)in 2022,continues to pose a significant threat due to the absence of vaccines or drugs for MPXV infection.In this study,we developed an mRNA vaccine that expressing the A29L antigen,a specific protein of the intracellular mature virus.Our vaccine utilizes a thermostable ionizable lipid nanoparticle(iLNP)platform and has been administered to mice.Our find-ings demonstrate that the MPXV A29L mRNA vaccine candidate induces robust cross-neutralizing immune responses against both vaccinia virus(VACV)and MPXV live virus.Furthermore,immunization with the vaccine candidate provided protection against the VACV challenge in mice.These findings underscore the potential of mRNA-LNP vaccines as safe and effective candidates against monkeypox epidemics.Given the current absence of specific interventions for MPXV infection,our study represents a significant step forward in developing a viable solution to combat this ongoing public health threat.展开更多
The occurrence and development of gastric cancer are regulated by many factors and result from the joint action of many factors. Studies have shown that ANP, NPRA, and Cx43 play a vital role in the proliferation and m...The occurrence and development of gastric cancer are regulated by many factors and result from the joint action of many factors. Studies have shown that ANP, NPRA, and Cx43 play a vital role in the proliferation and migration of gastric cancer. This article reviews the relationship between Atrial natriuretic peptide (ANP), Atrial natriuretic peptide receptor A (NPRA), and Connexin43 (Cx43) with the occurrence and development of gastric cancer. The review aims to provide an effective reference value for scientific research and clinical treatment.展开更多
Population size plays a crucial role in determining wheat yields.Altered carbohydrate accumulation resulting from increased competition between populations and individuals leads to poor-quality stems.The sowing date c...Population size plays a crucial role in determining wheat yields.Altered carbohydrate accumulation resulting from increased competition between populations and individuals leads to poor-quality stems.The sowing date can mitigate competition in densely planted populations.However,the underlying mechanism by which it confers resistance to wheat lodging remains elusive.In this study,Zimai 28(lodging-sensitive variety) and Shannong 28(lodging-resistant variety) were used with three sowing treatments on October 22(S1),October 28(S2),and November 3(S3).The sowing rate was adjusted to ensure adequate population size and consistency in the overwintering populations across sowing dates(300 plant m^(-2)for S1,375 plant m^(-2)for S2,and 525 plant m^(-2)for S3),The lodging resistance in winter wheat was increased by delayed sowing and increased sowing rate,which led to a reduction in tiller numbers and fostered primary stem development.A reduction in the overwinter cumulative temperature from 500 to 450℃,coupled with an elevation in sowing rates from 300 to 375 plant m^(-2)(transition from S1 to S2),corresponded with a notable increase in structural carbohydrates(lignin,cellulose,hemicellulose,and pectin) by 175.07 mg g^(-1).Additionally,there was a moderate increase in non-structural carbohydrates,including soluble sugars and starch,by 15.54 mg g^(-1).Delayed sowing and increased sowing rate elevated the precursor contents of lignin synthesis.Enhanced metabolic activity of related pathways ultimately increased dimer/trimer content.In summary,this study highlights the pivotal role of lignin metabolites and cross-linked structures in determining the stem stiffness breaking strength.展开更多
The Danjiangkou Reservoir is the water source for the middle route of the South-to-North Water Diversion Project in China. Thus, its water quality status is of great concern. Five water quality indicators （dissolved ...The Danjiangkou Reservoir is the water source for the middle route of the South-to-North Water Diversion Project in China. Thus, its water quality status is of great concern. Five water quality indicators （dissolved oxygen, permanganate index, ammonia nitrogen, total nitrogen, and total phosphorus）, were measured at three monitoring sites （the Danjiangkou Reservoir dam, the Hejiawan and the Jiangbei bridge）, to investigate changing trends, and spatiotemporal characteristics of water quality in the Danjiangkou Reservoir area from January 2006 to May 2012. We then applied a Bayesian statistical method to evaluate the water quality comprehensively. The normal distribution sampling method was used to calculate likelihood, and the entropy weight method was used to determine indicator weights for variables of interest in to the study. The results indicated that concentrations of all five indicators increased during the last six years. In addition, the water quality in the reservoir was worse during the wet season （from May to October）, than during the dry season （from November to April of the next year）. Overall, the probability of the water＇s belonging to quality category of type lI, according to environmental quality standards for surface water in China, was 27.7%-33.7%, larger than that of its belonging to the other four water quality types. The increasing concentrations of nutrients could result in eutrophication of the Danjiangkou Reser- voir. This method reduced the subjectivity that is commonly associated with determining indicator weights and artificial classifications, achieving more reliable results. These results indicate that it is important for the interbasin water diversion project to implement integrated water quality management in the Danjiangkou Reservoir area.展开更多
mRNA is a novel class of therapeutic modality that holds great promise in vaccination,protein replacement therapy,cancer immunotherapy,immune cell engineering etc.However,optimization of mRNA molecules and efficient i...mRNA is a novel class of therapeutic modality that holds great promise in vaccination,protein replacement therapy,cancer immunotherapy,immune cell engineering etc.However,optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application.Here we present an ionizable lipid nanoparticle(iLNP)based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs.mRNAs encoding luciferase or erythropoietin(EPO)were prepared by in vitro transcription and formulated with proposed iLNP,to form iLP171/mRNA formulations.It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes.The maximum amount of protein expression was found at 6 h post-administration.The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA.Moreover,no toxicity or immunogenicity was observed for these mRNA formulations.Therefore,our study provides a useful and promising platform for mRNA therapeutic development.展开更多
基金the State Grid Liaoning Electric Power Supply Co.,Ltd.(Research on Scheduling Decision Technology Based on Interactive Reinforcement Learning for Adapting High Proportion of New Energy,No.2023YF-49).
文摘Traditional optimal scheduling methods are limited to accurate physical models and parameter settings, which aredifficult to adapt to the uncertainty of source and load, and there are problems such as the inability to make dynamicdecisions continuously. This paper proposed a dynamic economic scheduling method for distribution networksbased on deep reinforcement learning. Firstly, the economic scheduling model of the new energy distributionnetwork is established considering the action characteristics of micro-gas turbines, and the dynamic schedulingmodel based on deep reinforcement learning is constructed for the new energy distribution network system with ahigh proportion of new energy, and the Markov decision process of the model is defined. Secondly, Second, for thechanging characteristics of source-load uncertainty, agents are trained interactively with the distributed networkin a data-driven manner. Then, through the proximal policy optimization algorithm, agents adaptively learn thescheduling strategy and realize the dynamic scheduling decision of the new energy distribution network system.Finally, the feasibility and superiority of the proposed method are verified by an improved IEEE 33-node simulationsystem.
基金supported by grants from Scientific Innovation 2030 Project (C.W,2022ZD0401703)National Key Research and Development Program of China (2021YFD1200700)+2 种基金National Natural Science Foundation of China (32372082)China Agriculture Research System (CARS-02-03)Innovation Program of Chinese Academy of Agricultural Sciences.
文摘Genotyping arrays based on single nucleotide polymorphisms(SNPs)provide a low-cost,highthroughput platform.The development of a SNP array that fully reflects the genetic diversity of maize(Zea mays L.)germplasm and is applicable to molecular breeding programs is desirable.In this study,we developed a MaizeGerm50K array comprising 50,852 SNPs selected from the resequencing data of 1604 maize inbred lines and other markers.A genome-wide association study using a landrace panel genotyped with the array permitted mapping of several known genes.We also verified a candidate gene,RNA-binding motif protein 24-like 1(ZmRBM24L1),delaying flowering through overexpression lines.Genomic selection for yield and agronomic traits showed high prediction accuracy.The MaizeGerm50K array is thus a valuable genomic tool for maize genetic studies and breeding.
基金supported by Science and Technology Innovation Programfor Postgraduate Students in IDP Subsidized by Fundamental Research Funds for the Central Universities(Project No.ZY20240335)support of the Research Project of the Key Technology of Malicious Code Detection Based on Data Mining in APT Attack(Project No.2022IT173)the Research Project of the Big Data Sensitive Information Supervision Technology Based on Convolutional Neural Network(Project No.2022011033).
文摘Previous studies have shown that deep learning is very effective in detecting known attacks.However,when facing unknown attacks,models such as Deep Neural Networks(DNN)combined with Long Short-Term Memory(LSTM),Convolutional Neural Networks(CNN)combined with LSTM,and so on are built by simple stacking,which has the problems of feature loss,low efficiency,and low accuracy.Therefore,this paper proposes an autonomous detectionmodel for Distributed Denial of Service attacks,Multi-Scale Convolutional Neural Network-Bidirectional Gated Recurrent Units-Single Headed Attention(MSCNN-BiGRU-SHA),which is based on a Multistrategy Integrated Zebra Optimization Algorithm(MI-ZOA).The model undergoes training and testing with the CICDDoS2019 dataset,and its performance is evaluated on a new GINKS2023 dataset.The hyperparameters for Conv_filter and GRU_unit are optimized using the Multi-strategy Integrated Zebra Optimization Algorithm(MIZOA).The experimental results show that the test accuracy of the MSCNN-BiGRU-SHA model based on the MIZOA proposed in this paper is as high as 0.9971 in the CICDDoS 2019 dataset.The evaluation accuracy of the new dataset GINKS2023 created in this paper is 0.9386.Compared to the MSCNN-BiGRU-SHA model based on the Zebra Optimization Algorithm(ZOA),the detection accuracy on the GINKS2023 dataset has improved by 5.81%,precisionhas increasedby 1.35%,the recallhas improvedby 9%,and theF1scorehas increasedby 5.55%.Compared to the MSCNN-BiGRU-SHA models developed using Grid Search,Random Search,and Bayesian Optimization,the MSCNN-BiGRU-SHA model optimized with the MI-ZOA exhibits better performance in terms of accuracy,precision,recall,and F1 score.
基金the project funding supported by the Beijing Nova Program(Z171100001117033)the Beijing Scholars Program(BSP041)the Youth Research Fund of BAAFS(QNJJ201931)。
文摘Genic male sterility(GMS) is one of the most important resources for exploiting heterosis in crop breeding, so that identifying genomic loci regulating GMS is desirable. However, many regulatory genes controlling GMS have not yet been characterized in maize, owing partly to a lack of genetic materials. We generated a recessive male-sterile maize mutant in the Jing 724 genetic background via ethyl methanesulfonate treatment, and found the male sterility to be due to a single gene mutation. Bulk-segregant RNA sequencing of three replicates indicated that one genomic region located at the end of chromosome 4 was associated with the observed mutant phenotype. Among genes with nonsynonymous mutations,Zm00001 d053895(bHLH51) showed abolished expression in the sterile bulks and was annotated as a bHLH transcription factor orthologous to Arabidopsis AMS, suggesting an association with the male sterility of the mutant. Kompetitive Allele-Specific PCR assays further validated the exclusive correlation of male sterility with the single C-to-T mutation in the fifth exon. The new maize mutant and the potential SNP locus provide novel genetic material for investigating the molecular mechanism underlying tapetal development and may facilitate the improvement of hybrid production systems.
基金Supported by National Natural Science Foundation of China(Grant No.51975168).
文摘Titanium alloy has been applied in the field of aerospace manufacturing for its high specific strength and hardness.Nonetheless,these properties also cause general problems in the machining,such as processing inefficiency,serious wear,poor workpiece face quality,etc.Aiming at the above problems,this paper carried out a comparative experimental study on titanium alloy milling based on the CAMCand BEMC.The variation law of cutting force and wear morphology of the two tools were obtained,and the wear mechanism and the effect of wear on machining quality were analyzed.The conclusion is that in contrast with BEMC,under the action of cutting thickness thinning mechanism,the force of CAMC was less,and its fluctuation was more stable.The flank wear was uniform and near the cutting edge,and the wear rate was slower.In the early period,the wear mechanism of CAMC was mainly adhesion.Gradually,oxidative wear also occurred with milling.Furthermore,the surface residual height of CAMC was lower.There is no obvious peak and trough accompanied by fewer surface defects.
基金This study was funded by the National Natural Science Foundation of China (Grant No. 72074164)Chinese Academy of Medical Sciences(Grant No. 2020-JKCS-024).
文摘Purpose:This study aimed to develop quality indicators for the care of older adults with disabilities in long-term care facilities(LTCFs)based on Maslow’s hierarchy of needs.Methods:The draft of the quality indicators was drawn up based on a literature review and research group discussion.The quality indicators were finalized by two rounds of expert consultation(involving 15 experts)using the Delphi method.The Analytic Hierarchy Process was applied to calculate the indicators’weight.Results:The response rates of the two rounds of consultation were 100%and 93%,and the expert authority coefficients were 0.86 and 0.87.After two rounds of consultation,the expert opinion coordination coefficients of the first-,second-and third-level indicators were 0.42,0.25,and 0.96,respectively(P<0.05),and the variation coefficient was0.25.The final quality indicators for the care of older adults with disabilities in LTCFs included 7 first-level,19 second-level,and 107 third-level indicators.Conclusion:The quality indicators for the care of older adults with disabilities in LTCFs are reliable,scientific,comprehensive,and practical and specify the content of person-centered care needs.This can provide a reference for evaluating and improving care quality in LTCFs.
基金the financial support from the National Natural Science Foundation of China(22078058)。
文摘Swirling addition to the stream is beneficial for the fluid mixing.This work aims to study the mixing process intensification in a conventional T-jets mixer by the swirling addition.After experimental verification by the planar laser-induced fluorescence technique,large eddy simulation with the dynamic kinetic energy sub-grid stress model is used to predict how the swirling strength(in terms of swirling number,S_(w))and swirling directions affect the mixing performance,e.g.the tracer concentration distribution,mixing time,and turbulent characteristics in the T-jets mixers,Predictions show that the swirling strength is the key factor affecting the mixing efficiency of the process.The overall mixing time,τ_(90),can be significantly reduced by increasing S_(w).Vortex analysis shows that more turbulent eddies appear in the collision zone and the turbulent kinetic energy dissipation rate increases obviously with the swirling addition.When S_(w) is kept constant,the mixing process can be accelerated and intensified by adding swirling to only one stream,to both streams with the opposite swirling directions,or to both streams with the same swirling directions.Amplification of the mixing process by enlarging the mixer size or increasing the flow rates is also optimized.Thus,this work provides a new strategy to improve the mixing performance of the traditional T-jets mixers by the swirling addition.
文摘[ Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patu/a L. [ Method] By using tissue culture tech- nology, different mass fractions of 6-BA and NAA were added to MS medium to compare the effect of different culture medium on the rapid propagation of T. patu/a L. [Result] Shoot tips or stem segments of T. patu/a L. were used as explants for tissue culture with an appropriate sterilization time of 8 min; differentiation effect of shoot tips was better than that of stem segments; callus generation rate was high with the high content of growth regulators; MS medium containing O. 1 mg/L NAA and 1.5 rag/L 6-BA was used for subculture proliferation with a subculture period of 4 weeks; rooting rate of plantlet was the maximum （97%） in 1/2MS medium containing 0.2 mg/L NAA, and the root system was relatively developed. [ Conclusion] This study provided technical support for the industrialized seedling breeding of T. patula L.
基金Supported by Science and Technology Program of Guangzhou City(2016201604030076)Science and Technology Program of Guangdong Province(2014A020208062)+2 种基金Cooperation Project of Industry,Education and Academy of Dongguan City(2015509105105)the Fund for Demonstration and Extension of Introduced Intellectual Achievements in Guangdong "Forcing Cultivation and Flowering Regulation Technology Introduction,Demonstration and Promotion in Hippeastrum vittatum" and "The Characteristics of Taiwan Varieties and Cultivation Technology Introduction and Promotion in Hippeastrum vittatum"Open Project of Key Laboratory of Urban Agriculture in South China,Ministry of Agriculture,China
文摘In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog （MS） medium supplemented with 0.5 mg/L N-phenyl-N＇-1,2,3-thiadiazol-5-ylurea （TDZ）, 1.0 mg/L 2,4-diehloro- phenoxyacetic acid （2,4-D）, and 30 g/L sucrose （ pH5. 8） in the dark at 25 ±1℃. Further study of the influence of kanamyein and hygromycin on callus induction and multiplication showed that, the lethal doses of kanamycin and hygromycin to bulb explants were 100 and 30 mg/L, respectively. All explants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage, and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg,/L hygromyein, and all the calli died at last. These results will provide important reference for further studies of trausgenic H. vittaturn.
基金supported by the Medical Science Research Program of Hebei Province(20211020).
文摘Objective:To investigate the role of KIF3C gene in promoting the malignant phenotype of lung cancer cells and in regulating PI3K/AKT signaling pathway.Methods:CCK-8 and transwell assays were used to detect the changes in cell proliferation and cell migration ability after being transfected with siKIF3C,as well as the protein expression levels of PI3K,p-PI3K,AKT,and p-AKT following the downregulation of KIF3C by Western blot.Results:The CCK-8 assay showed that the proliferation/viability of lung cancer cells A549 significantly reduced after being transfected with siKIF3C gene(P<0.05);the migration ability of lung cancer cells A549 was significantly reduced after transfected with siKIF3C gene(P<0.05);the levels of p-PI3K and p-AKT proteins were downregulated after KIF3C protein knockdown(P<0.05);however,the detection of PI3K and AKT protein levels was not statistically significant.Conclusion:KIF3C may promote the proliferation and migration ability of lung cancer cells A549 through PI3K/AKT signaling pathway.
基金Medical Science Research Program of Hebei Province(Project no.20211020).
文摘The objective of this study is to reveal the role of KIF3C gene in the proliferation of lung cancer cells,and the regulation of epithelial mesenchymal transition(EMT)of tumor cells.The plate clone formation assay and cell scratch assay were used in this study to detect the changes of cell proliferation and migration ability after siKIF3C interference,while EMT-related protein expression after KIF3C downregulation was detected by Western blot.The cell clone formation assay showed that the number of clones of lung cancer cells A549 was significantly reduced after transfected with siKIF3C(P<0.05);The scratch assay showed that the healing ability of cells was significantly reduced after transfected with siKIF3C(P<0.05);Western blot protein analysis revealed that the levels of EMT-related proteins,N-cadherin,Vimentin,Snail,and Slug were significantly down-regulated(P<0.05),however,E-cadherin protein levels were up-regulated after siKIF3C interference.In conclusion,KIF3C may promote the proliferation and invasive ability of lung cancer cells A549 through EMT.
基金supported by the Project of National Natural Science Foundation of China under the grant titled“Research on Intermittent Fault Diagnosis of New Interconnection Networks under Comparative Model”(Approval Number:61862003).
文摘In the context of enterprise systems,intrusion detection(ID)emerges as a critical element driving the digital transformation of enterprises.With systems spanning various sectors of enterprises geographically dispersed,the necessity for seamless information exchange has surged significantly.The existing cross-domain solutions are challenged by such issues as insufficient security,high communication overhead,and a lack of effective update mechanisms,rendering them less feasible for prolonged application on resource-limited devices.This study proposes a new cross-domain collaboration scheme based on federated chains to streamline the server-side workload.Within this framework,individual nodes solely engage in training local data and subsequently amalgamate the final model employing a federated learning algorithm to uphold enterprise systems with efficiency and security.To curtail the resource utilization of blockchains and deter malicious nodes,a node administration module predicated on the workload paradigm is introduced,enabling the release of surplus resources in response to variations in a node’s contribution metric.Upon encountering an intrusion,the system triggers an alert and logs the characteristics of the breach,facilitating a comprehensive global update across all nodes for collective defense.Experimental results across multiple scenarios have verified the security and effectiveness of the proposed solution,with no loss of its recognition accuracy.
基金supported by the National Natural Science Foundation(32001008,32171394,31901053,32101157,32101148,82202338,China)the National Key Research&Development Program of China(2021YFA1201000,2021YFE0106900,2021YFC2302400)+1 种基金the Fundamental Research Funds for the Central Universities(2022CX01013,China)Beijing Nova Program(Interdisciplinary Cooperation Project)from Beijing Municipal Science&Technology Commission(20220484207,China).
文摘Small interfering RNA(siRNA)has a promising future in the treatment of ocular diseases due to its high efficiency,specificity,and low toxicity in inhibiting the expression of target genes and proteins.However,due to the unique anatomical structure of the eye and various barriers,delivering nucleic acids to the retina remains a significant challenge.In this study,we rationally design PACD,an A-B-C type non-viral vector copolymer composed of a hydrophilic PEG block(A),a siRNA binding block(B)and a pH-responsive block(C).PACDs can self-assemble into nanosized polymeric micelles that compact siRNAs into polyplexes through simple mixing.By evaluating its pH-responsive activity,gene silencing efficiency in retinal cells,intraocular distribution,and anti-angiogenesis therapy in a mouse model of hypoxia-induced angiogenesis,we demonstrate the efficiency and safety of PACD in delivering siRNA in the retina.We are surprised to discover that,the PACD/siRNA polyplexes exhibit remarkable intracellular endosomal escape efficiency,excellent gene silencing,and inhibit retinal angiogenesis.Our study provides design guidance for developing efficient nonviral ocular nucleic acid delivery systems.
基金supported by National Key Research&Development Program of China(Nos.2021YFA1201000,2021YFC2302400)Beijing Institute of Technology Research Fund Program for Young Scholars(No.XSQD-6120220072)+1 种基金National Natural Science Foundation of China(No.82371846)China Postdoctoral Science Foundation(No.2022M720438).
文摘The monkeypox virus(MPXV)outbreak,declared a Public Health Emergency of International Concern(PHEIC)by the World Health Organization(WHO)in 2022,continues to pose a significant threat due to the absence of vaccines or drugs for MPXV infection.In this study,we developed an mRNA vaccine that expressing the A29L antigen,a specific protein of the intracellular mature virus.Our vaccine utilizes a thermostable ionizable lipid nanoparticle(iLNP)platform and has been administered to mice.Our find-ings demonstrate that the MPXV A29L mRNA vaccine candidate induces robust cross-neutralizing immune responses against both vaccinia virus(VACV)and MPXV live virus.Furthermore,immunization with the vaccine candidate provided protection against the VACV challenge in mice.These findings underscore the potential of mRNA-LNP vaccines as safe and effective candidates against monkeypox epidemics.Given the current absence of specific interventions for MPXV infection,our study represents a significant step forward in developing a viable solution to combat this ongoing public health threat.
文摘The occurrence and development of gastric cancer are regulated by many factors and result from the joint action of many factors. Studies have shown that ANP, NPRA, and Cx43 play a vital role in the proliferation and migration of gastric cancer. This article reviews the relationship between Atrial natriuretic peptide (ANP), Atrial natriuretic peptide receptor A (NPRA), and Connexin43 (Cx43) with the occurrence and development of gastric cancer. The review aims to provide an effective reference value for scientific research and clinical treatment.
基金supported by the National Natural Science Foundation of China(32172117,32101834)the Shandong Province Agricultural Major Technology Collaborative Promotion Plan Project(SDNYXTTG-2023-33)+1 种基金Postdoctoral Science Foundation of China(2022M711968)the Natural Science Foundation of Shandong Province(ZR2020QC106).
文摘Population size plays a crucial role in determining wheat yields.Altered carbohydrate accumulation resulting from increased competition between populations and individuals leads to poor-quality stems.The sowing date can mitigate competition in densely planted populations.However,the underlying mechanism by which it confers resistance to wheat lodging remains elusive.In this study,Zimai 28(lodging-sensitive variety) and Shannong 28(lodging-resistant variety) were used with three sowing treatments on October 22(S1),October 28(S2),and November 3(S3).The sowing rate was adjusted to ensure adequate population size and consistency in the overwintering populations across sowing dates(300 plant m^(-2)for S1,375 plant m^(-2)for S2,and 525 plant m^(-2)for S3),The lodging resistance in winter wheat was increased by delayed sowing and increased sowing rate,which led to a reduction in tiller numbers and fostered primary stem development.A reduction in the overwinter cumulative temperature from 500 to 450℃,coupled with an elevation in sowing rates from 300 to 375 plant m^(-2)(transition from S1 to S2),corresponded with a notable increase in structural carbohydrates(lignin,cellulose,hemicellulose,and pectin) by 175.07 mg g^(-1).Additionally,there was a moderate increase in non-structural carbohydrates,including soluble sugars and starch,by 15.54 mg g^(-1).Delayed sowing and increased sowing rate elevated the precursor contents of lignin synthesis.Enhanced metabolic activity of related pathways ultimately increased dimer/trimer content.In summary,this study highlights the pivotal role of lignin metabolites and cross-linked structures in determining the stem stiffness breaking strength.
基金Acknowledgements This study was supported by the National Science and Technology Support Program （No. 2011BAC12B02）, the National Science Foundation for Innovative Research Group （No. 51121003）, and the Outstanding Doctoral Thesis Fund of Beijing Normal University （No. 105512GK）. We are grateful to the editors and anonymous reviewers＇ careful review of this paper, whom have contributed substantially to improving the paper.
文摘The Danjiangkou Reservoir is the water source for the middle route of the South-to-North Water Diversion Project in China. Thus, its water quality status is of great concern. Five water quality indicators （dissolved oxygen, permanganate index, ammonia nitrogen, total nitrogen, and total phosphorus）, were measured at three monitoring sites （the Danjiangkou Reservoir dam, the Hejiawan and the Jiangbei bridge）, to investigate changing trends, and spatiotemporal characteristics of water quality in the Danjiangkou Reservoir area from January 2006 to May 2012. We then applied a Bayesian statistical method to evaluate the water quality comprehensively. The normal distribution sampling method was used to calculate likelihood, and the entropy weight method was used to determine indicator weights for variables of interest in to the study. The results indicated that concentrations of all five indicators increased during the last six years. In addition, the water quality in the reservoir was worse during the wet season （from May to October）, than during the dry season （from November to April of the next year）. Overall, the probability of the water＇s belonging to quality category of type lI, according to environmental quality standards for surface water in China, was 27.7%-33.7%, larger than that of its belonging to the other four water quality types. The increasing concentrations of nutrients could result in eutrophication of the Danjiangkou Reser- voir. This method reduced the subjectivity that is commonly associated with determining indicator weights and artificial classifications, achieving more reliable results. These results indicate that it is important for the interbasin water diversion project to implement integrated water quality management in the Danjiangkou Reservoir area.
基金supported by the National Natural Science Foundation of China(31871003,81402863,31901053)the Beijing Institute of Technology Research Fund Program for Young Scholars and the Fundamental Research Funds for the Central Universities(3052018065,1870012222004)+2 种基金the Natural Science Foundation of Guangdong Province(2019A1515010776)the Hunan Provincial Natural Science Foundation of China(2018JJ1019,2019JJ50196)the Hu-Xiang Young Talent Program(2018RS3094).
文摘mRNA is a novel class of therapeutic modality that holds great promise in vaccination,protein replacement therapy,cancer immunotherapy,immune cell engineering etc.However,optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application.Here we present an ionizable lipid nanoparticle(iLNP)based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs.mRNAs encoding luciferase or erythropoietin(EPO)were prepared by in vitro transcription and formulated with proposed iLNP,to form iLP171/mRNA formulations.It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes.The maximum amount of protein expression was found at 6 h post-administration.The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA.Moreover,no toxicity or immunogenicity was observed for these mRNA formulations.Therefore,our study provides a useful and promising platform for mRNA therapeutic development.
