The static sealing of underground gas storage(UGS),including the integrity of cap rocks and the stability of faults,is analyzed from a macro perspective using a comprehensive geological evaluation method.Changes in po...The static sealing of underground gas storage(UGS),including the integrity of cap rocks and the stability of faults,is analyzed from a macro perspective using a comprehensive geological evaluation method.Changes in pore structure,permeability,and mechanical strength of cap rocks under cyclic loads may impact the rock sealing integrity during the injection and recovery phases of UGS.In this work,the mechanical deformation and failure tests of rocks,as well as rock damage tests under alternating loads,are conducted to analyze the changes in the strength and permeability of rocks under multiple-cycle intense injection and recovery of UGS.Additionally,this study proposes an evaluation method for the dynamic sealing performance of UGS cap rocks under multi-cycle alternating loads.The findings suggest that the failure strength(70%)can be used as the critical value for rock failure,thus providing theoretical support for determining the upper limit of operating pressure and the number of injection-recovery cycles for the safe operation of a UGS system.展开更多
Owing to its photonic band gap(PBG)and slow light effects,aniline black(AB)-poly(vinylidene fluoride)(PVDF)inverse opal(IO)photonic crystal(PC)was constructed to promote the utility of light and realize photothermal s...Owing to its photonic band gap(PBG)and slow light effects,aniline black(AB)-poly(vinylidene fluoride)(PVDF)inverse opal(IO)photonic crystal(PC)was constructed to promote the utility of light and realize photothermal synergetic catalysis.As a highly efficient reaction platform with the capability of restricting heat,a microreactor was introduced to further amplify the photothermal effects of near infrared(NIR)radiation.The photocatalytic efficiency of ZnO/0.5AB-PVDF IO(Z0.5A)increases 1.63-fold compared to that of pure ZnO film under a full solar spectrum,indicating the effectiveness of synergetic promotion by slow light and photothermal effects.Moreover,a 5.85-fold increase is achieved by combining Z0.5A with a microreactor compared to the film in a beaker.The photon localization effect of PVDF IO was further exemplified by finite-difference time-domain(FDTD)calculations.In conclusion,photonic crystal-microreactor enhanced photothermal catalysis has immense potential for alleviating the deteriorating water environment.展开更多
Photocatalytic conversion of CO_(2)into solar fuels provides a bright route for the green and sustainable development of human society.However,the realization of efficient photocatalytic CO_(2)reduction reaction(CO_(2...Photocatalytic conversion of CO_(2)into solar fuels provides a bright route for the green and sustainable development of human society.However,the realization of efficient photocatalytic CO_(2)reduction reaction(CO_(2)RR)is still challenging owing to the sluggish kinetics or unfavorable thermodynamics for basic chemical processes of CO_(2)RR,such as adsorption,activation,conversion and product desorption.To overcome these shortcomings,recent works have demonstrated that surface engineering of semiconductors,such as introducing surface vacancy,surface doping,and cocatalyst loading,serves as effective or promising strategies for improved photocatalytic CO_(2)RR with high activity and selectivity.The essential reason lies in the activation and reaction pathways can be optimized and regulated through the reconstruction of surface atomic and electronic structures.Herein,in this review,we focus on recent research advances about rational design of semiconductor surface for photocatalytic CO_(2)RR.The surface engineering strategies for improved CO_(2)adsorption,activation,and product selectivity will be reviewed.In addition,theoretical calculations along with in situ characterization techniques will be in the spotlight to clarify the kinetics and thermodynamics of the reaction process.The aim of this review is to provide deep understanding and rational guidance on the design of semiconductors for photocatalytic CO_(2)RR.展开更多
This research aims to investigate the effect of frost damage on chloride transportation mechanism in ordinary andfiber concrete with both theoretical and experimental methods.The proposed theoretical model takes into a...This research aims to investigate the effect of frost damage on chloride transportation mechanism in ordinary andfiber concrete with both theoretical and experimental methods.The proposed theoretical model takes into account the varying damage levels caused by concrete cover depth and freeze-thaw cycles,which are the two primary parameters affecting the expression of the chloride diffusion coefficient.In the experiment,three types of concrete were prepared:ordinary Portland concrete(OPC),polypropylenefiber concrete(PFC),and steelfiber concrete(SFC).These were then immersed in NaCl solution for 120 days after undergoing 10,25,and 50 freeze-thaw cycles.The damage coefficient of the tested concrete was determined by measuring the dynamic elas-tic modulus.The results indicated that the relative dynamic elasticity modulus of the specimens decreased with each freeze-thaw cycle,and the chloride diffusion coefficient of the specimens increased as the degree of frost degradation increased.Samples containing steel and polypropylenefibers exhibited greater resistance to cyclic water freezing compared to the controlled concrete withoutfibers.A model has been also developed that takes into account the damage caused by freezing-thawing cycles and the depth of the concrete,which can predict variations in free chloride concentration at different depths.The calculated values were in good agreement with the test results for depths between 10 to 30 mm.This new damage-induced diffusion model can helpfill the gap in research on the effects of freeze-thaw cycles on chloride diffusion.展开更多
Integrating hierarchical plasmonic cavities into photocatalysis offers a promising avenue for expanding the light utilization range to cover the entire solar spectrum. However, fabricating these nanostructures with se...Integrating hierarchical plasmonic cavities into photocatalysis offers a promising avenue for expanding the light utilization range to cover the entire solar spectrum. However, fabricating these nanostructures with seamless size transitions for a wide plasmon resonant range remains technically challenging, requiring precise nanofabrication control and often relying on expensive and laborious techniques like e-beam lithography and reactive ion etching. Herein, a one-step forming strategy was explored to fabricate simple yet hierarchical plasmonic cavities featuring the surface nanodome array-integrated plasmonic Fabry–Pérot cavity through a facile large-area nanoimprinting method. This design leverages a uniform feature size and periodic arrangement to broaden the light utilization range of TiO_(2) across the entire solar spectrum (200–2500 nm). It consists of an upper nanodome array cavity with vertically continuous graded sizes for broadband absorption (200–1500 nm), coupled with a bottom plate cavity that enlarges the overall cavity size to extend the range to 2500 nm. Remarkably, simply adjusting the thickness of the plate cavity can tune the resonant position, eliminating the need for expensive mold modifications. When combined with TiO_(2), this hierarchical plasmonic cavity significantly enhances the photocatalytic hydrogen evolution rate to 36.3 µmol/h, achieving a remarkable 9.8-fold increase compared to pure TiO_(2) under full-spectrum illumination. This approach offers a convenient and inexpensive alternative to sophisticated nanofabrication techniques for large-area hierarchical plasmonic cavities with broadband plasmon resonance to enhance the photocatalytic hydrogen evolution.展开更多
The development of low-cost and high-active cocatalysts is one of the most significant links for photocatalytic water splitting.Herein,a novel strategy of electron delocalization modulation for transition metal sulfid...The development of low-cost and high-active cocatalysts is one of the most significant links for photocatalytic water splitting.Herein,a novel strategy of electron delocalization modulation for transition metal sulfides has been developed by anion hybridization.P-modified CoS_(2)(CoS_(2)|P)nanocrystals were firstly fabricated via a gas-solid reaction and coupled with CdS nanorods to construct a composite catalyst for solar H2 evolution reaction(HER).The CdS/CoS_(2)|P catalyst shows an HER rate of 57.8 pmol h-1,which is 18 times that of the bare CdS,8 times that of the CdS/CoS2,and twice that of Pt/CdS.The reduced energy barrier and suppressed reverse reaction for HER on the catalyst have been predicted and explained by density functional theory(DFT)calculation.The underlying design strategy of novel cocatalysts by electron delocalization modulation may shed light on the rational development of other advanced catalysts for energy conversion.展开更多
Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nuc...Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.展开更多
Dual inhibition of glycolysis and oxidative phosphorylation(OXPHOS)can break the metabolic plasticity of cancer cells to inhibit most energy supply and lead to effective cancer therapy.However,the pharmacokinetic diff...Dual inhibition of glycolysis and oxidative phosphorylation(OXPHOS)can break the metabolic plasticity of cancer cells to inhibit most energy supply and lead to effective cancer therapy.However,the pharmacokinetic difference among drugs hinders these two inhibitions to realize a uniform temporal and spatial distribution.Herein,we report an aptamer-based artificial enzyme for simultaneous dual inhibition of glycolysis and OXPHOS,which is constructed by arginine aptamer modified carbon-dots-doped graphitic carbon nitride(AptCCN).AptCCN can circularly capture intracellular arginine attribute to the specific binding ability of arginine aptamers to arginine,and further catalyze the oxidation of enriched arginine to nitric oxide(NO)under red light irradiation.In vitro and in vivo experiments showed that arginine depletion and NO stress could inhibit glycolysis and OXPHOS,leading to energy blockage and apoptosis of cancer cells.The presented aptamer-based artificial enzyme strategy provides a new path for cell pathway regulation and synergistic cancer therapy.展开更多
Small molecule aptamers discovered by traditional selection methods usually lack conformational changes upon target binding.This limits the use of aptamers as molecular probes for small molecule detection and regulato...Small molecule aptamers discovered by traditional selection methods usually lack conformational changes upon target binding.This limits the use of aptamers as molecular probes for small molecule detection and regulatory elements of genetic circuits.Here,we report a new method called capture and in vitro transcription-systematic evolution of ligands by exponential enrichment(CIVT-SELEX)to select DNA aptamers that can not only bind to small molecule ligands but also undergo significant conformational changes.Through this method,we select a structure-switching aptamer of uridine-5′-diphosphate(UDP).Taking advantage of its conformational changes,we first construct a UDP-responsive transcriptional switch by inserting the aptamer in a genetic circuit and demonstrate that it can respond to the addition of UDP and regulate the transcription of downstream genes.We also build a UDP aptamer-based biosensor that can be used for active glycosyltransferase screening.We believe this method can provide a universal platform for selecting small molecule aptamers with conformational changes and expand the use of aptamers in small molecule detection and genetic regulation.展开更多
Development of low-cost and efficient photocatalytic materials with visible-light response is of urgent need for solving energy and environmental problems.Here,a metal-free two-dimensional(2D)π-conjugated hybrid g-C_...Development of low-cost and efficient photocatalytic materials with visible-light response is of urgent need for solving energy and environmental problems.Here,a metal-free two-dimensional(2D)π-conjugated hybrid g-C_(3)N_(4)photocatalyst with tunable band structure was prepared by a novel one-pot bottom-up method based on a supersaturated precipitation process of urea and triethanolamine(TEOA)solution.The microstructure of the hybrid g-C_(3)N_(4)is revealed to be a compound of periodic tri-s-triazine units grafted with N-doped graphene(GR)fragments.From experimental evidence and theoretical calculations,the two differentπ-conjugated fragments in the hybrid g-C_(3)N_(4)material are proved to construct a 2D in-plane junction structure,thereby expanding the light absorption range and accelerating the interface charge transfer.Theπ-conjugated electron coupling in the 2D photocatalyst eliminates the grain boundary effect,and the coupled highest occupied molecular orbital(HOMO)effectively promotes the separation of photo-induced charge carriers.Compared with the g-C_(3)N_(4)prepared by the conventional method,the visible-light H2 production activity of the optimized sample is enhanced by 253%.This work provides a new strategy of constructing metal-free g-C_(3)N_(4)hybrids for efficient photocatalytic water splitting.展开更多
Chronic myeloid leukemia(CML)is a hematopoietic malignancy driven by the fusion gene BCR::ABL1.Drug resistance to tyrosine kinase inhibitors(TKIs),due to BCR::ABL1 mutations and residual leukemia stem cells(LSCs),rema...Chronic myeloid leukemia(CML)is a hematopoietic malignancy driven by the fusion gene BCR::ABL1.Drug resistance to tyrosine kinase inhibitors(TKIs),due to BCR::ABL1 mutations and residual leukemia stem cells(LSCs),remains a major challenge in CML treatment.Here,we revealed the requirement of the vitamin D receptor(VDR)in the progression of CML.VDR was upregulated by BCR::ABL1 and highly expressed in CML cells.Interestingly,VDR knockdown inhibited the proliferation of CML cells driven by both BCR::ABL1 and TKI-resistant BCR::ABL1 mutations.Mechanistically,VDR transcriptionally regulated DDIT4 expression;reduced DDIT4 levels upon VDR knockdown triggered DNA damage and senescence via p53 signaling activation in CML cells.Furthermore,VDR deficiency not only suppressed tumor burden and progression in primary CML mice but also reduced the self-renewal capacity of CML-LSCs.Together,our study demonstrated that targeting VDR is a promising strategy to overcome TKI resistance and eradicate LSCs in CML.展开更多
文摘The static sealing of underground gas storage(UGS),including the integrity of cap rocks and the stability of faults,is analyzed from a macro perspective using a comprehensive geological evaluation method.Changes in pore structure,permeability,and mechanical strength of cap rocks under cyclic loads may impact the rock sealing integrity during the injection and recovery phases of UGS.In this work,the mechanical deformation and failure tests of rocks,as well as rock damage tests under alternating loads,are conducted to analyze the changes in the strength and permeability of rocks under multiple-cycle intense injection and recovery of UGS.Additionally,this study proposes an evaluation method for the dynamic sealing performance of UGS cap rocks under multi-cycle alternating loads.The findings suggest that the failure strength(70%)can be used as the critical value for rock failure,thus providing theoretical support for determining the upper limit of operating pressure and the number of injection-recovery cycles for the safe operation of a UGS system.
文摘Owing to its photonic band gap(PBG)and slow light effects,aniline black(AB)-poly(vinylidene fluoride)(PVDF)inverse opal(IO)photonic crystal(PC)was constructed to promote the utility of light and realize photothermal synergetic catalysis.As a highly efficient reaction platform with the capability of restricting heat,a microreactor was introduced to further amplify the photothermal effects of near infrared(NIR)radiation.The photocatalytic efficiency of ZnO/0.5AB-PVDF IO(Z0.5A)increases 1.63-fold compared to that of pure ZnO film under a full solar spectrum,indicating the effectiveness of synergetic promotion by slow light and photothermal effects.Moreover,a 5.85-fold increase is achieved by combining Z0.5A with a microreactor compared to the film in a beaker.The photon localization effect of PVDF IO was further exemplified by finite-difference time-domain(FDTD)calculations.In conclusion,photonic crystal-microreactor enhanced photothermal catalysis has immense potential for alleviating the deteriorating water environment.
基金financially supported by JSPS KAKENHI(JP18H02065)Photo-excitonic Project in Hokkaido University,National Natural Science Foundation of China(21633004,22002060,and 51872138)+4 种基金Natural Science Foundation of Jiangsu Province(BK20181380)Qing Lan Project,Six Talent Peaks Project in Jiangsu Province(XCL-029)Priority Academic Program Development of the Jiangsu Higher Education Institutions(PAPD)the support provided by the China Scholarships Council(202008320109)China Postdoctoral Science Foundation(2020M681564)。
文摘Photocatalytic conversion of CO_(2)into solar fuels provides a bright route for the green and sustainable development of human society.However,the realization of efficient photocatalytic CO_(2)reduction reaction(CO_(2)RR)is still challenging owing to the sluggish kinetics or unfavorable thermodynamics for basic chemical processes of CO_(2)RR,such as adsorption,activation,conversion and product desorption.To overcome these shortcomings,recent works have demonstrated that surface engineering of semiconductors,such as introducing surface vacancy,surface doping,and cocatalyst loading,serves as effective or promising strategies for improved photocatalytic CO_(2)RR with high activity and selectivity.The essential reason lies in the activation and reaction pathways can be optimized and regulated through the reconstruction of surface atomic and electronic structures.Herein,in this review,we focus on recent research advances about rational design of semiconductor surface for photocatalytic CO_(2)RR.The surface engineering strategies for improved CO_(2)adsorption,activation,and product selectivity will be reviewed.In addition,theoretical calculations along with in situ characterization techniques will be in the spotlight to clarify the kinetics and thermodynamics of the reaction process.The aim of this review is to provide deep understanding and rational guidance on the design of semiconductors for photocatalytic CO_(2)RR.
基金supported by the Graduate Research Innovation Program of Jiangsu University(Si Y.D.,SJCX21_1689)the Foundation from the National Natural Science Foundation of China(Yan Y.D.,51608233).
文摘This research aims to investigate the effect of frost damage on chloride transportation mechanism in ordinary andfiber concrete with both theoretical and experimental methods.The proposed theoretical model takes into account the varying damage levels caused by concrete cover depth and freeze-thaw cycles,which are the two primary parameters affecting the expression of the chloride diffusion coefficient.In the experiment,three types of concrete were prepared:ordinary Portland concrete(OPC),polypropylenefiber concrete(PFC),and steelfiber concrete(SFC).These were then immersed in NaCl solution for 120 days after undergoing 10,25,and 50 freeze-thaw cycles.The damage coefficient of the tested concrete was determined by measuring the dynamic elas-tic modulus.The results indicated that the relative dynamic elasticity modulus of the specimens decreased with each freeze-thaw cycle,and the chloride diffusion coefficient of the specimens increased as the degree of frost degradation increased.Samples containing steel and polypropylenefibers exhibited greater resistance to cyclic water freezing compared to the controlled concrete withoutfibers.A model has been also developed that takes into account the damage caused by freezing-thawing cycles and the depth of the concrete,which can predict variations in free chloride concentration at different depths.The calculated values were in good agreement with the test results for depths between 10 to 30 mm.This new damage-induced diffusion model can helpfill the gap in research on the effects of freeze-thaw cycles on chloride diffusion.
基金supported by the National Natural Science Foundation of China(No.52272082)the General Project of Natural Science Foundation of Universities and Colleges in Jiangsu Province(No.22KJB430026)The Priority Academic Program Development of the Jiangsu Higher Education Institutions(PAPD)is gratefully acknowledged.
文摘Integrating hierarchical plasmonic cavities into photocatalysis offers a promising avenue for expanding the light utilization range to cover the entire solar spectrum. However, fabricating these nanostructures with seamless size transitions for a wide plasmon resonant range remains technically challenging, requiring precise nanofabrication control and often relying on expensive and laborious techniques like e-beam lithography and reactive ion etching. Herein, a one-step forming strategy was explored to fabricate simple yet hierarchical plasmonic cavities featuring the surface nanodome array-integrated plasmonic Fabry–Pérot cavity through a facile large-area nanoimprinting method. This design leverages a uniform feature size and periodic arrangement to broaden the light utilization range of TiO_(2) across the entire solar spectrum (200–2500 nm). It consists of an upper nanodome array cavity with vertically continuous graded sizes for broadband absorption (200–1500 nm), coupled with a bottom plate cavity that enlarges the overall cavity size to extend the range to 2500 nm. Remarkably, simply adjusting the thickness of the plate cavity can tune the resonant position, eliminating the need for expensive mold modifications. When combined with TiO_(2), this hierarchical plasmonic cavity significantly enhances the photocatalytic hydrogen evolution rate to 36.3 µmol/h, achieving a remarkable 9.8-fold increase compared to pure TiO_(2) under full-spectrum illumination. This approach offers a convenient and inexpensive alternative to sophisticated nanofabrication techniques for large-area hierarchical plasmonic cavities with broadband plasmon resonance to enhance the photocatalytic hydrogen evolution.
基金supported by the National Natural Science Foundation of China(Nos.51872138 and 22002060)Natural Science Foundation of Jiangsu Province(No.BK20181380)+2 种基金Qing Lan Project,Six Talent Peaks Project in Jiangsu Province(No.XCL-029)Priority Academic Program Development of the Jiangsu Higher Education Institutions(PAPD).Dr.Hengming Huang gratefully acknowledges the support provided by China Scholarships Council(CSC No.202008320109)China Postdoctoral Science Foundation(No.2020M681564).
文摘The development of low-cost and high-active cocatalysts is one of the most significant links for photocatalytic water splitting.Herein,a novel strategy of electron delocalization modulation for transition metal sulfides has been developed by anion hybridization.P-modified CoS_(2)(CoS_(2)|P)nanocrystals were firstly fabricated via a gas-solid reaction and coupled with CdS nanorods to construct a composite catalyst for solar H2 evolution reaction(HER).The CdS/CoS_(2)|P catalyst shows an HER rate of 57.8 pmol h-1,which is 18 times that of the bare CdS,8 times that of the CdS/CoS2,and twice that of Pt/CdS.The reduced energy barrier and suppressed reverse reaction for HER on the catalyst have been predicted and explained by density functional theory(DFT)calculation.The underlying design strategy of novel cocatalysts by electron delocalization modulation may shed light on the rational development of other advanced catalysts for energy conversion.
文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided.
基金supported by the National Key Research and Development Program of China(Nos.2018YFA0902600 and 2020YFA0210800)the National Natural Science Foundation of China(Nos.22174019,21775025,U1705281,and 22027805)+1 种基金the Natural Science Foundation of Fujian(No.2020J06036)the Major Project of Science and Technology of Fujian Province(No.2020HZ06006).
文摘Dual inhibition of glycolysis and oxidative phosphorylation(OXPHOS)can break the metabolic plasticity of cancer cells to inhibit most energy supply and lead to effective cancer therapy.However,the pharmacokinetic difference among drugs hinders these two inhibitions to realize a uniform temporal and spatial distribution.Herein,we report an aptamer-based artificial enzyme for simultaneous dual inhibition of glycolysis and OXPHOS,which is constructed by arginine aptamer modified carbon-dots-doped graphitic carbon nitride(AptCCN).AptCCN can circularly capture intracellular arginine attribute to the specific binding ability of arginine aptamers to arginine,and further catalyze the oxidation of enriched arginine to nitric oxide(NO)under red light irradiation.In vitro and in vivo experiments showed that arginine depletion and NO stress could inhibit glycolysis and OXPHOS,leading to energy blockage and apoptosis of cancer cells.The presented aptamer-based artificial enzyme strategy provides a new path for cell pathway regulation and synergistic cancer therapy.
基金supported by the National Natural Science Foundation of China(32001037,22176035)the National Key R&D Program of China(2020YFA0210800,2018YFA0902600)+1 种基金the Natural Science Foundation of Fujian Province(2020J01491,2020J05120)Fuzhou University Research Fund(GXRC-20033)。
文摘Small molecule aptamers discovered by traditional selection methods usually lack conformational changes upon target binding.This limits the use of aptamers as molecular probes for small molecule detection and regulatory elements of genetic circuits.Here,we report a new method called capture and in vitro transcription-systematic evolution of ligands by exponential enrichment(CIVT-SELEX)to select DNA aptamers that can not only bind to small molecule ligands but also undergo significant conformational changes.Through this method,we select a structure-switching aptamer of uridine-5′-diphosphate(UDP).Taking advantage of its conformational changes,we first construct a UDP-responsive transcriptional switch by inserting the aptamer in a genetic circuit and demonstrate that it can respond to the addition of UDP and regulate the transcription of downstream genes.We also build a UDP aptamer-based biosensor that can be used for active glycosyltransferase screening.We believe this method can provide a universal platform for selecting small molecule aptamers with conformational changes and expand the use of aptamers in small molecule detection and genetic regulation.
基金financially supported by National Natural Science Foundation of China(Nos.22002060 and 51872138)Natural Science Foundation of Jiangsu Province(No.BK20181380)+3 种基金Qing Lan Project,Six Talent Peaks Project in Jiangsu Province(No.XCL029)Priority Academic Program Development of the Jiangsu Higher Education Institutions(PAPD)support provided by China Scholarships Council(CSC No.202008320109)China Postdoctoral Science Foundation(No.2020M681564)。
文摘Development of low-cost and efficient photocatalytic materials with visible-light response is of urgent need for solving energy and environmental problems.Here,a metal-free two-dimensional(2D)π-conjugated hybrid g-C_(3)N_(4)photocatalyst with tunable band structure was prepared by a novel one-pot bottom-up method based on a supersaturated precipitation process of urea and triethanolamine(TEOA)solution.The microstructure of the hybrid g-C_(3)N_(4)is revealed to be a compound of periodic tri-s-triazine units grafted with N-doped graphene(GR)fragments.From experimental evidence and theoretical calculations,the two differentπ-conjugated fragments in the hybrid g-C_(3)N_(4)material are proved to construct a 2D in-plane junction structure,thereby expanding the light absorption range and accelerating the interface charge transfer.Theπ-conjugated electron coupling in the 2D photocatalyst eliminates the grain boundary effect,and the coupled highest occupied molecular orbital(HOMO)effectively promotes the separation of photo-induced charge carriers.Compared with the g-C_(3)N_(4)prepared by the conventional method,the visible-light H2 production activity of the optimized sample is enhanced by 253%.This work provides a new strategy of constructing metal-free g-C_(3)N_(4)hybrids for efficient photocatalytic water splitting.
基金supported by grants from the National Natural Science Foundation of China(81874294)the Taishan Scholars Program(TSQN201812015)+3 种基金the program for Multidisciplinary Research and Innovation Team of Young Scholars of Shandong University(2020QNQT007)the key Program of the Natural Science Foundation of Shandong Province(ZR2022LSW027)This work was also supported by the program for Innovative Research Team at the University of Ministry of Education of China(IRT_17R68)the Foundation for Innovative Research Groups of State Key Laboratory of Microbial Technology(WZCX2021-03).
文摘Chronic myeloid leukemia(CML)is a hematopoietic malignancy driven by the fusion gene BCR::ABL1.Drug resistance to tyrosine kinase inhibitors(TKIs),due to BCR::ABL1 mutations and residual leukemia stem cells(LSCs),remains a major challenge in CML treatment.Here,we revealed the requirement of the vitamin D receptor(VDR)in the progression of CML.VDR was upregulated by BCR::ABL1 and highly expressed in CML cells.Interestingly,VDR knockdown inhibited the proliferation of CML cells driven by both BCR::ABL1 and TKI-resistant BCR::ABL1 mutations.Mechanistically,VDR transcriptionally regulated DDIT4 expression;reduced DDIT4 levels upon VDR knockdown triggered DNA damage and senescence via p53 signaling activation in CML cells.Furthermore,VDR deficiency not only suppressed tumor burden and progression in primary CML mice but also reduced the self-renewal capacity of CML-LSCs.Together,our study demonstrated that targeting VDR is a promising strategy to overcome TKI resistance and eradicate LSCs in CML.
