Root-associated microbiota profoundly affect crop health and productivity.Plants can selectively recruit beneficial microbes from the soil and actively balance microbe-triggered plant-growth promotion and stress toler...Root-associated microbiota profoundly affect crop health and productivity.Plants can selectively recruit beneficial microbes from the soil and actively balance microbe-triggered plant-growth promotion and stress tolerance enhancement.The cost associated with this is the root-mediated support of a certain number of specific microbes under nutrient limitation.Thus,it is important to consider the dynamic changes in microbial quantity when it comes to nutrient condition-induced root microbiome reassembly.Quantitative microbiome profiling(QMP)has recently emerged as a means to estimate the specific microbial load variation of a root microbiome(instead of the traditional approach quantifying relative microbial abundances)and data from the QMP approach can be more closely correlated with plant development and/or function.However,due to a lack of detailed-QMP data,how soil nutrient conditions affect quantitative changes in microbial assembly of the root-associated microbiome remains poorly understood.A recent study quantified the dynamics of the soybean root microbiome,under unbalanced fertilization,using QMP and provided data on the use of specific synthetic communities(SynComs)for sustaining crop productivity.In this editorial,we explore potential opportunities for utilizing QMP to decode the microbiome for sustainable agriculture.展开更多
Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To de...Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide （NO） and brassinolide （BL） in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. ALl2 enhanced the activities of total protein phosphorylation, Ca2^-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte- induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.展开更多
Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales....Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales.Here,we surveyed 494 pairwise fungal communities in leaves and soils by Illumina sequencing,which were associated with 55 woody plant species across more than 2,000-km span of mountain forests in eastern China.The relative contributions of plant,climate,soil and space to the variation of fungal communities were assessed,and the plant-fungus network topologies were inferred.Plant phylogeny was the strongest predictor for fungal community composition in leaves,accounting for 19.1%of the variation.In soils,plant phylogeny,climatic factors and soil properties explained 9.2%,9.0%and 8.7%of the variation in soil fungal community,respectively.The plant-fungus networks in leaves exhibited significantly higher specialization,modularity and robustness(resistance to node loss),but less complicated topology(e.g.,significantly lower linkage density and mean number of links)than those in soils.In addition,host/fungus preference combinations and key species,such as hubs and connectors,in bipartite networks differed strikingly between aboveground and belowground samples.The findings provide novel insights into cross-kingdom(plant-fungus)species co-occurrence at large spatial scales.The data further suggest that community shifts of trees due to climate change or human activities will impair aboveground and belowground forest fungal diversity in different ways.展开更多
Compound biological bait can replace commercial bait to ensure fish growth.The compound biogenic bait can effectively improve the water and soil environment.The key microbiome induced by compound biogenic bait plays a...Compound biological bait can replace commercial bait to ensure fish growth.The compound biogenic bait can effectively improve the water and soil environment.The key microbiome induced by compound biogenic bait plays an important role.Traditional commercial aquatic fish bait(CA)is not conducive to the scientific breeding of rice and fish in cocropping systems,and excessive feeding easily causes environmental pollution in rice fields.In this study,an environment-friendly compound biogenic bait(CB)mixed with plant-derived(PB)and animal-derived(AB)baits was proposed.The rice–crucian carp cocropping system was used as the research object,and the soil microorganisms and fish gut microorganisms were sequenced with high throughput,respectively,to verify the effect of CB application and the microbial mechanism underlying its functional effect.The results showed that the AB and PB components in CB maintain the growth of fish by improving the metabolism-related functions of fish gut microbiome and reducing the abundance of intestinal pathogenic bacteria,including Actinomadura.In particular,the PB components induced soil microbiome,such as Pseudonocardia,that participate in soil nutrient cycling and increase dissolved oxygen in water,which is key for improving rice quality and yield.This is the first study to focus on how different bait components drive key microbial communities to regulate animal–plant–environment relationships in the integrated planting and breeding patterns of paddy fields.展开更多
基金supported by grants from the National Natural Science Foundation of China(grant nos.32301332,32071638 and 32100227)a project funded by the Priority Academic Program Development(PAPD)of the Jiangsu Higher Education Institutions of China.
文摘Root-associated microbiota profoundly affect crop health and productivity.Plants can selectively recruit beneficial microbes from the soil and actively balance microbe-triggered plant-growth promotion and stress tolerance enhancement.The cost associated with this is the root-mediated support of a certain number of specific microbes under nutrient limitation.Thus,it is important to consider the dynamic changes in microbial quantity when it comes to nutrient condition-induced root microbiome reassembly.Quantitative microbiome profiling(QMP)has recently emerged as a means to estimate the specific microbial load variation of a root microbiome(instead of the traditional approach quantifying relative microbial abundances)and data from the QMP approach can be more closely correlated with plant development and/or function.However,due to a lack of detailed-QMP data,how soil nutrient conditions affect quantitative changes in microbial assembly of the root-associated microbiome remains poorly understood.A recent study quantified the dynamics of the soybean root microbiome,under unbalanced fertilization,using QMP and provided data on the use of specific synthetic communities(SynComs)for sustaining crop productivity.In this editorial,we explore potential opportunities for utilizing QMP to decode the microbiome for sustainable agriculture.
基金the National Natural Science Foundation of China (No. 31070443 and No. 30970523)
文摘Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide （NO） and brassinolide （BL） in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. ALl2 enhanced the activities of total protein phosphorylation, Ca2^-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte- induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.
基金supported by the NSFC-NSF Dimensions of Biodiversity Program(31461123001)the National Natural Science Foundation of China(41907039,42277308)+3 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(XDA28020202)the National Key R&D Program of China(2022YFD1500202)the US National Science Foundation(DEB-1442280)to PSS and DESthe China Biodiversity Observation Network(Sino BON)。
文摘Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales.Here,we surveyed 494 pairwise fungal communities in leaves and soils by Illumina sequencing,which were associated with 55 woody plant species across more than 2,000-km span of mountain forests in eastern China.The relative contributions of plant,climate,soil and space to the variation of fungal communities were assessed,and the plant-fungus network topologies were inferred.Plant phylogeny was the strongest predictor for fungal community composition in leaves,accounting for 19.1%of the variation.In soils,plant phylogeny,climatic factors and soil properties explained 9.2%,9.0%and 8.7%of the variation in soil fungal community,respectively.The plant-fungus networks in leaves exhibited significantly higher specialization,modularity and robustness(resistance to node loss),but less complicated topology(e.g.,significantly lower linkage density and mean number of links)than those in soils.In addition,host/fungus preference combinations and key species,such as hubs and connectors,in bipartite networks differed strikingly between aboveground and belowground samples.The findings provide novel insights into cross-kingdom(plant-fungus)species co-occurrence at large spatial scales.The data further suggest that community shifts of trees due to climate change or human activities will impair aboveground and belowground forest fungal diversity in different ways.
基金supported by the National Natural Science Foundation of China(Grant No.32001210)the Opening Project of Jiangsu Province Engineering Research Center of Agricultural and Rural Pollution Prevention Technology and Equipment(Grant No.GCZXZD2301)the Natural Science Foundation of the Jiangsu Higher Education Institution of China(Grant No.22KJB180003).
文摘Compound biological bait can replace commercial bait to ensure fish growth.The compound biogenic bait can effectively improve the water and soil environment.The key microbiome induced by compound biogenic bait plays an important role.Traditional commercial aquatic fish bait(CA)is not conducive to the scientific breeding of rice and fish in cocropping systems,and excessive feeding easily causes environmental pollution in rice fields.In this study,an environment-friendly compound biogenic bait(CB)mixed with plant-derived(PB)and animal-derived(AB)baits was proposed.The rice–crucian carp cocropping system was used as the research object,and the soil microorganisms and fish gut microorganisms were sequenced with high throughput,respectively,to verify the effect of CB application and the microbial mechanism underlying its functional effect.The results showed that the AB and PB components in CB maintain the growth of fish by improving the metabolism-related functions of fish gut microbiome and reducing the abundance of intestinal pathogenic bacteria,including Actinomadura.In particular,the PB components induced soil microbiome,such as Pseudonocardia,that participate in soil nutrient cycling and increase dissolved oxygen in water,which is key for improving rice quality and yield.This is the first study to focus on how different bait components drive key microbial communities to regulate animal–plant–environment relationships in the integrated planting and breeding patterns of paddy fields.
