Intestinal immune homeostasis plays a critical role in the pathogenesis of food allergy.However,the association between intestinal microenvironment factors and food allergy severity is not well studied.In this study,w...Intestinal immune homeostasis plays a critical role in the pathogenesis of food allergy.However,the association between intestinal microenvironment factors and food allergy severity is not well studied.In this study,we established a gluten allergy(GA)BALB/c mouse model and revealed the intestinal luminal factor-mediated alterations in phenotypes and endotypes of GA,combined with untargeted metabolomic profiling of the colonic contents.Our results showed that gluten sensitization induced severe allergic responses in BALB/c mice,characterized by exacerbated clinical allergic and diarrheal symptoms,increased histamine,elevated gluten-specific immunoglobulin(Ig)E and IgG2a levels,and increased mast cell degranulation.In response to GA,T-cell balance was disrupted,with aberrant production of interleukin(IL)-4,interferon(IFN)-γ,IL-10,and IL-2 in the spleen.GA led to a disrupted intestinal microenvironment homeostasis,including increased pH and water content,impaired intestinal antioxidant capacity and epithelial barrier function,decreased short-chain fatty acid production,and microbial dysbiosis,which was strongly correlated with GA severity.By metabolomic profiling,we found 29 differential expressed metabolites(DEMs)associated with GA,with 9 down-regulated and 20 up-regulated.A total of 11 out of all DEMs were classified into dipeptides,and 10 of them were up-regulated in the GA mice.Pathway enrichment analysis showed that most of the DEMs were enriched into the bile secretion metabolic route.展开更多
Emerging evidence indicated that the increase in food allergy(FA)over the past few decades was associated with the abnormal compositional and metabolic changes of gut microbiota.Gut microbiota played a vital role in m...Emerging evidence indicated that the increase in food allergy(FA)over the past few decades was associated with the abnormal compositional and metabolic changes of gut microbiota.Gut microbiota played a vital role in maintaining the homeostasis of the immune system and the dysbiosis of gut microbiota promoted the occurrence of FA.Recent research suggested that short-chain fatty acids(SCFAs),the main metabolites derived from gut microbiota,contributed to FA protection.Herein,we provided a comprehensive review on the relationship between gut microbiota and FA.The multifaceted mechanisms underlymg beneficial effects of gut microbiota composition/metabolites on the regulation of diverse cellular pathways in intestinal epithelial cells,dendritic cells,innate lymphoid cells,T cells,B cells and mast cells in the immune system were discussed systematically.These findings emphasized the positive function of gut microbiota in FA and provided novel ideas for the treatment or prevention of FA in the future.展开更多
Monitoring of subsurface fluid （underground fluid） is an important part of efforts for earthquake prediction in China. The nationwide network, which monitors groundwater level, water temperature, and radon and mercu...Monitoring of subsurface fluid （underground fluid） is an important part of efforts for earthquake prediction in China. The nationwide network, which monitors groundwater level, water temperature, and radon and mercury in groundwater, has been constructed in the last decades. Large amounts of abnormal fluid changes before and after major earthquakes have been recorded, providing precious data for research in earthquake sciences. Many studies have been done in earthquake fluid hydrogeology in order to probe the nature of the earthquake. Much progress in earthquake fluid hydrogeology has been made in the last decades. The paper provides a review of the advances in research on earthquake fluid hydrogeology over the last 40 years in China. It deals with the following five aspects： （1） an introduction to the development history of monitoring networks construction; （2） cases of different subsurface fluid changes recorded before some major earthquakes which occurred in the last decades; （3） characteristics of subsurface fluid changes following major earthquakes; （4） mechanism of subsurface fluid changes before and following earthquakes; （5） application of earthquake fluids in the hydrogeology field.展开更多
With the prevalence of food allergy increasing every year,food allergy has become a common public health problem.More and more studies have shown that probiotics can intervene in food allergy based on the intestinal m...With the prevalence of food allergy increasing every year,food allergy has become a common public health problem.More and more studies have shown that probiotics can intervene in food allergy based on the intestinal mucosal immune system.Probiotics and their metabolites can interact with immune cells and gut microbiota to alleviate food allergy.This review outlines the relationship between the intestinal mucosal immune system and food allergy.This review also presents the clinical application and potential immunomodulation mechanisms of probiotics on food allergy.We aim at providing a reference for further studies to explore the key active substances and immunomodulation mechanisms of anti-allergic probiotics.展开更多
Objective:Histology grade,subtypes and TNM stage of lung adenocarcinomas are useful predictors of prognosis and survival.The aim of the study was to investigate the relationship between chromosomal instability,morphol...Objective:Histology grade,subtypes and TNM stage of lung adenocarcinomas are useful predictors of prognosis and survival.The aim of the study was to investigate the relationship between chromosomal instability,morphological subtypes and the grading system used in lung non-mucinous adenocarcinoma(LNMA).Methods:We developed a whole genome copy number variation(WGCNV)scoring system and applied next generation sequencing to evaluate CNVs present in 91 LNMA tumor samples.Results:Higher histological grades,aggressive subtypes and more advanced TNM staging were associated with an increased WGCNV score,particularly in CNV regions enriched for tumor suppressor genes and oncogenes.In addition,we demonstrate that 24-chromosome CNV profiling can be performed reliably from specific cell types(<100 cells)isolated by sample laser capture microdissection.Conclusions:Our findings suggest that the WGCNV scoring system we developed may have potential value as an adjunct test for predicting the prognosis of patients diagnosed with LNMA.展开更多
Increasing incidences showed that food allergies have attracted more and more attention from researchers.BALB/c mice were sensitized with wheat gluten combined with aluminum hydroxide adjuvant via intraperitoneal inje...Increasing incidences showed that food allergies have attracted more and more attention from researchers.BALB/c mice were sensitized with wheat gluten combined with aluminum hydroxide adjuvant via intraperitoneal injection,transdermal sensitization,and oral gavage sensitization route.Results showed that all the three sensitization methods could induce allergic symptoms;increase the serum antibody(total immunoglobulin E(IgE),specific IgE,IgG,IgA)and histamine content;promote the secretion of Th2 cytokines(interleukin(IL)-4,IL-5,IL-13)and inflammatory factors(IL-6,IL-17 A,IL-10);and inhibit the production of Th1 cytokines(IFN-γ,IL-2).However,the allergic symptoms of mice sensitized by intraperitoneal injection were the most obvious among the three models.The level of serum antibodies in intraperitoneal injection group was significantly higher than control.Subsequently,16 S rRNA sequencing technology was used to analyze the intestinal flora of mice.The results showed that the abundance of Firmicutes in the wheat protein sensitized group was lower than that in the normal group,while the abundance of Bacteroidetes was higher,and Lactobacillus was the difference marker in normal group.Bacterial species diversity analysis showed that the species richness and diversity of intestinal flora in mice were decreased,the difference between mice induced by intraperitoneal injection and normal control group mice was the most significant.Taken together,these results show that among three sensitization methods used to build a mouse model with aluminum hydroxide as adjuvant,intraperitoneal injection is the comparably best way to build a mouse sensitization mode.展开更多
The conjecture of twin prime numbers is a mathematical problem. Proving the twin prime conjecture using traditional modern number theory is extremely profound and complex. We propose an elementary research method for ...The conjecture of twin prime numbers is a mathematical problem. Proving the twin prime conjecture using traditional modern number theory is extremely profound and complex. We propose an elementary research method for corresponding prime number, proved that the conjecture of twin prime numbers and obtain the corresponding prime distribution equation. According to the distribution rate of corresponding prime numbers, the distribution pattern of twin prime numbers was proved the distribution rate theorem. This is the distribution rate of prime numbers corresponding to composite numbers, which approaches the distribution rate of prime numbers corresponding to integers. Based on the corresponding prime distribution equation, obtain the twin prime inequality function. Then, the formula for calculating twin prime numbers was discussed. There is also the Hardy Littlewood conjecture. This provides a practical and feasible approach for studying the distribution of twin prime numbers.展开更多
We have examined the water level data from 16 wells in Sichuan province,China,recorded before the Wenchuan Ms8.0 earthquake occurred in 2008.We found that the data of 5 among these wells exhibit possible precursory an...We have examined the water level data from 16 wells in Sichuan province,China,recorded before the Wenchuan Ms8.0 earthquake occurred in 2008.We found that the data of 5 among these wells exhibit possible precursory anomalies,which are respectively named the Chuan No.08 well in Deyang,Chuan No.ll well in Pujiang,Chuan No.13 well in Luzhou,Chuan No.22 well in Qionglai and Beichuan.In time durations,these anomalies are of long-,intermediate-and short-term signals which are primarily distributed on the northeast(NE)trending Longmenshan fault zone as well as the parallel Huayingshan fault zone.It seems that the variations of the well levels on the Huayingshan fault zone imply compression while those on the Longmenshan fault zone reflect extension.These anomalies occurred first in the areas outside the epicenter region of the Wenchuan Ms8.0 earthquake,then migrated to the seismogenic fault zone.And they migrated from southwest to northeast in agreement with the rupture process of the main shock.展开更多
We systematically analyze coseismic responses and post-seismic characteristics of groundwater levels in the Three Gorges well-network to the Ms8.0 Wenchuan earthquake on 12 May 2008. The results indicate that these ch...We systematically analyze coseismic responses and post-seismic characteristics of groundwater levels in the Three Gorges well-network to the Ms8.0 Wenchuan earthquake on 12 May 2008. The results indicate that these characteristics differ among wells. On the conditions of similar borehole configurations, the differences are associated with geological structural sites of wells, burial types of aquifers monitored, and transmissivities of aquifer systems. We explored coseismic and post-seismic step-rise and step-drop mechanical mechanisms and their implication to earthquake prediction. We validated the inference that the residual step-rise zone is a possible earthquake risk zone based on recent seismic activity on the Xiannüshan fault in the area.展开更多
Theoretically,propagating internal tides in the ocean may reflect at turning depths,where buoyancy frequencies equal tidal frequencies,before colliding with the air-sea interface or rugged bottom topography.Globally,t...Theoretically,propagating internal tides in the ocean may reflect at turning depths,where buoyancy frequencies equal tidal frequencies,before colliding with the air-sea interface or rugged bottom topography.Globally,the internal tide lower turning depths(ITLTDs)in the open ocean have been mapped;however,knowledge of the presence of ITLTDs in the South China Sea(SCS)is lacking.In this study,2125 high-quality temperature-salinity profiles(including 58 deep-sea hydrographic measurements with observational depths exceeding 3000 m)are collected and analyzed to investigate the existence of ITLTDs in the SCS.Furthermore,the concept of the upper turning depth is first introduced in the context of internal tides,and internal tide upper turning depths(ITUTDs)are also investigated.ITLTDs are found to exist at several abyssal stations;these stations are distributed mostly in the southern part of the SCS basin,possibly due to the greater water depths there.Fewer locations show the presence of ITLTDs for K_(1) versus M_(2) tidal frequencies because of the lower tidal frequency.The distance between ITLTDs and the seafloor ranged from 270 m to more than 1200 m,implying the possible existence of multiple internal wave evanescent regions in the abyssal bottom.ITUTDs of tens of meters are ubiquitous in the SCS;stations with the presence of ITUTDs are located mainly in the northeastern SCS due to the intensive observations there.However,the calculated ITUTDs have large uncertainties;they are sensitive to the selected bin values.The horizontal propagation directions of internal tides in the SCS change dramatically,and as a result,the estimated turning depths under the full Coriolis force definition are different compared to that under the traditional approximation.展开更多
Wheat flour,as the most important source of food globally,is one of the most common causative agents of food allergy.This study aimed to investigate the effects of fermentation on wheat protein digestibility and aller...Wheat flour,as the most important source of food globally,is one of the most common causative agents of food allergy.This study aimed to investigate the effects of fermentation on wheat protein digestibility and allergenicity.Protein digestibility were evaluated using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and enzyme-linked immunosorbent assay.The effect of protein on intestinal permeability was investigated by Caco-2 cell monolayers.Co-culture fermentation with Pediococcus acidilactici XZ31 and yeast leads to improvement in digestibility of wheat protein compared to single strain fermentation.Fermentation leads to a decrease in albumin/globulin antigenicity and an increase in gluten R5 reactivity,with the most significant changes in the co-culture group.Digestion strengthen the decrease of protein antigenicity and counteracts the difference in antigenicity induced by fermentation between groups.However,pretreatment with P.acidilactici XZ31 reduces the amount of allergens across Caco-2 monolayer and attenuates the gluten-induced increase in permeability of Caco-2 cell monolayer by reducing actin polymerization and villous atrophy.Co-culture fermentation reduces gluten-induced cell monolayer damage to a greater extent than P.acidilactici XZ31 monoculture.These results gives valuable insight into the effects of P.acidilactici XZ31 fermentation on the allergenicity and toxicity of wheat proteins,which contribute to promoting the application of multi-strain leavening agent in hypoallergenic and gluten-free wheat products.展开更多
Molecular weaving is a powerful approach to make molecularly woven materials that have showed unprecedented characteristics and properties intrinsically distinct to those of non-woven materials.We here report a facile...Molecular weaving is a powerful approach to make molecularly woven materials that have showed unprecedented characteristics and properties intrinsically distinct to those of non-woven materials.We here report a facile and efficient approach for the synthesis of 2D woven supramolecular polymers by differentiated self-assembly through orthogonal noncovalent interactions.Importantly,the difference in binding strength of two orthogonal noncovalent interactions can be used to control the process of molecular weaving.Consequently,single-layered 2D woven supramolecular polymers were synthesized and fully characterized by various techniques.This study demonstrates a controllable method for molecular weaving,and will significantly hasten the development of molecularly woven materials.展开更多
Conventional type 1 dendritic cells(cDC1)are the essential antigen-presenting DC subset in antitumor immunity.Suppressing B-cell lymphoma 9 and B-cell lymphoma 9-like(BCL9/BCL9L)inhibits tumor growth and boosts immune...Conventional type 1 dendritic cells(cDC1)are the essential antigen-presenting DC subset in antitumor immunity.Suppressing B-cell lymphoma 9 and B-cell lymphoma 9-like(BCL9/BCL9L)inhibits tumor growth and boosts immune responses against cancer.However,whether oncogenic BCL9/BCL9L impairs antigen presentation in tumors is still not completely understood.Here,we show that targeting BCL9/BCL9L enhanced antigen presentation by stimulating cDC1 activation and infiltration into tumor.Pharmacological inhibition of BCL9/BCL9L with a novel inhibitor hsBCL9z96 or Bcl9/Bcl9l knockout mice markedly delayed tumor growth and promoted antitumor CD8^(+)T cell responses.Mechanistically,targeting BCL9/BCL9L promoted antigen presentation in tumors.This is due to the increase of cDC1 activation and tumor infiltration by the XCL1-XCR1 axis.Importantly,using single-cell transcriptomics analysis,we found that Bcl9/Bcl9l deficient cDC1 were superior to wild-type(WT)cDC1 at activation and antigen presentation via NF-κB/IRF1 signaling.Together,we demonstrate that targeting BCL9/BCL9L plays a crucial role in cDC1-modulated antigen presentation of tumor-derived antigens,as well as CD8^(+)T cell activation and tumor infiltration.Targeting BCL9/BCL9L to regulate cDC1 function and directly orchestrate a positive feedback loop necessary for optimal antitumor immunity could serve as a potential strategy to counter immune suppression and enhance cancer immunotherapy.展开更多
[Objectives] To investigate the species diversity of wood-decaying fungi in karst regions of southwest China. [Methods] The karst core regions of southwest China, specifically Guizhou, Guangxi, and Yunnan, were invest...[Objectives] To investigate the species diversity of wood-decaying fungi in karst regions of southwest China. [Methods] The karst core regions of southwest China, specifically Guizhou, Guangxi, and Yunnan, were investigated for the first time to analyze the species diversity characteristics of wood-decaying fungi in this area. This analysis was conducted through a comprehensive 5-year field investigation and systematic identification process. [Results] The wood-decaying fungi exhibited a distinctive species composition and distribution pattern within karst habitats, encompassing significant groups such as Polyporaceae and Hymenochaetaceae. Furthermore, these fungi demonstrated varying functional characteristics across the two ecological types of standing tree decay and wood decay. It was observed that wood-decaying fungi had a significant impact on the material cycle within karst ecosystems by degrading lignin and cellulose. Furthermore, the distribution of species diversity was closely associated with the characteristics of karst geomorphology and the types of vegetation. [Conclusions] This study not only addresses the lack of background data regarding wood-decaying fungi resources in karst regions, but also elucidates the maintenance mechanisms of fragile ecosystems from the perspective of decomposers. It provides a scientific foundation for biodiversity conservation, the prevention and control of forest diseases, and sustainable ecological management in karst regions.展开更多
Vehicle model recognition(VMR)benefits the parking,surveillance,and tolling system by automatically identifying the exact make and model of the passing vehicles.Edge computing technology enables the roadside facilitie...Vehicle model recognition(VMR)benefits the parking,surveillance,and tolling system by automatically identifying the exact make and model of the passing vehicles.Edge computing technology enables the roadside facilities and mobile cameras to achcieve VMR in realtime.Current work generally relies on a specific view of the vehicle or requires huge calculation capability to deploy the end-to-end deep learning network.This paper proposes a lightweight two-stage identification method based on object detection and image retrieval techniques,which empowers us the ability of recognizing the vehicle model from an arbitrary view.The first-stage model estimates the vehicle posture using object detection and similarity matching,which is cost-efficient and suitable to be programmed in the edge computing devices;the second-stage model retrieves the vehicle’s label from the dataset based on gradient boosting decision tree(GBDT)algorithm and VGGNet,which is flexible to the changing dataset.More than 8000 vehicle images are labeled with their components’information,such as headlights,windows,wheels,and logos.The YOLO network is employed to detect and localize the typical components of a vehicle.The vehicle postures are estimated by the spatial relationship between different segmented components.Due to the variety of the perspectives,a 7-dimensional vector is defined to represent the relative posture of the vehicle and screen out the images with a similar photographic perspective.Two algorithms are used to extract the features from each image patch:(1)the scale invariant feature transform(SIFT)combined with the bag-of-features(BoF)and(2)pre-trained deep neural network.The GBDT is applied to evaluate the weight of each component regarding its impact on VMR.The descriptors of each component are then aggregated to retrieve the best matching image from the database.The results showed its advantages in terms of accuracy(89.2%)and efficiency,demonstrating the vast potential of applying this method to large-scale vehicle model recognition.展开更多
Effective oil replenishment to the lubrication track of a running bearing is crucial to its sustainable operation.Reliable practical solutions are rare despite numerous theoretical studies were conducted in the last f...Effective oil replenishment to the lubrication track of a running bearing is crucial to its sustainable operation.Reliable practical solutions are rare despite numerous theoretical studies were conducted in the last few decades.This paper proposes the use of surface effect,wettability gradient,to achieve the goal.This method is simple and can be nicely implemented using femtosecond laser ablation.A periodic comb-tooth-shaped pattern with anisotropic wetting capability is devised and its effect on the anisotropic spreading behaviour of an oil droplet is studied.Results show that the comb-tooth-shaped pattern enables the rearrangement of oil distribution,thereby escalating oil replenishment to the lubrication track.The effect is due to the unbalanced interfacial force created by the surface pattern.The influence of the shape and the pitch of teeth,which are the two governing factors,on oil transport is also reported.The effects of the newly devised surface pattern on lubrication are experimentally evaluated under the conditions of limited lubricant supply.These results are promising,demonstrating the reduction in bearing friction and the increase in lubricating film thickness.展开更多
Neutral aqueous rechargeable Co_(3)O_(4)//Zn batteries with high-output voltage and outstanding cycling stability have yielded new insights into wearable energy-storage devices.To meet the increasing demand for a mean...Neutral aqueous rechargeable Co_(3)O_(4)//Zn batteries with high-output voltage and outstanding cycling stability have yielded new insights into wearable energy-storage devices.To meet the increasing demand for a means of powering wearable and portable devices,the development of a high-performance fiber-shaped Co//Zn battery would be highly desirable.However,the intrinsically poor conductivity of C 03O4 significantly restricts the application of these high-capacity and high-rate aqueous rechargeable battery.Encouragingly,density functional theory(DFT)calculations demonstrate that the substitution of Zn for Co^(3+)leads to an insulatormetal transition in the Zn-doped Co_(3)O_(4)(Zn-Co_(3)O_(4)).In this study,we used metallic Zn-Co_(3)O_(4)nanowire arrays(NWAs)as a novel binder-free cathode to successfully fabricate an all-solid-state fiber-shaped aqueous rechargeable(AFAR)Co//Zn battery.The resulting fiber-shaped Co//Zn battery takes advantage of the enhanced conductivity,increased capacity,and improved rate capability of Zn-Co_(3)O_(4)NWAs to yield a remarkable capacity of 1.25 mAh·cm^(-2)at a current density of 0.5 mA·cm^(-2),extraordinary rate capability(60.8%capacity retention at a high current density of 20 mA·cm^(-2))and an admirable energy density of 772.6 mWh·cm^(-3).Thus,the successful construction of Zn-Co_(3)O_(4)NWAs provides valuable insights into the design of high-capacity and high-rate cathode materials for aqueous rechargeable high-voltage batteries.展开更多
Fiber-shaped energy storage devices are indispensable parts of wearable and portable electronics.Aqueous rechargeable Ni/Fe battery is a very appropriate energy storage device due to their good safety without organic ...Fiber-shaped energy storage devices are indispensable parts of wearable and portable electronics.Aqueous rechargeable Ni/Fe battery is a very appropriate energy storage device due to their good safety without organic electrolytes, high ionic conductivity, and low cost. Unfortunately, the low energy density,poor power density and cycling performance hinder its further practical applications. In this study, in order to obtain high performance negative iron-based material, we first synthesized a-iron oxide(α-Fe2O3) nanorods(NRs) with superstructures on the surface of highly conductive carbon nanotube fibers(CNTFs), then electrically conductive polypyrrole(PPy) was coated to enhance the electron, ion diffusion and cycle stability. The as-prepared α-Fe2O3@PPy NRs/CNTF electrode shows a high specific capacity of 0.62 Ah cm-3 at the current density of 1 A cm-3. Furthermore, the Ni/Fe battery that was assembled by the above negative electrode shows a maximum volumetric energy density of 15.47 mWh cm-3 with228.2 mW cm-3 at a current density of 1 A cm-3. The cycling durability and mechanical flexibility of the Ni/Fe battery were tested, which show good prospect for practical application. In summary, these merits make it possible for our Ni/Fe battery to have practical applications in next generation flexible energy storage devices.展开更多
基金financially supported by the State Key Research and Development Plan(2019YFC1605000).
文摘Intestinal immune homeostasis plays a critical role in the pathogenesis of food allergy.However,the association between intestinal microenvironment factors and food allergy severity is not well studied.In this study,we established a gluten allergy(GA)BALB/c mouse model and revealed the intestinal luminal factor-mediated alterations in phenotypes and endotypes of GA,combined with untargeted metabolomic profiling of the colonic contents.Our results showed that gluten sensitization induced severe allergic responses in BALB/c mice,characterized by exacerbated clinical allergic and diarrheal symptoms,increased histamine,elevated gluten-specific immunoglobulin(Ig)E and IgG2a levels,and increased mast cell degranulation.In response to GA,T-cell balance was disrupted,with aberrant production of interleukin(IL)-4,interferon(IFN)-γ,IL-10,and IL-2 in the spleen.GA led to a disrupted intestinal microenvironment homeostasis,including increased pH and water content,impaired intestinal antioxidant capacity and epithelial barrier function,decreased short-chain fatty acid production,and microbial dysbiosis,which was strongly correlated with GA severity.By metabolomic profiling,we found 29 differential expressed metabolites(DEMs)associated with GA,with 9 down-regulated and 20 up-regulated.A total of 11 out of all DEMs were classified into dipeptides,and 10 of them were up-regulated in the GA mice.Pathway enrichment analysis showed that most of the DEMs were enriched into the bile secretion metabolic route.
基金financially supported by the National Key Research and Development Program of China(2019YFC1605000)the National Natural Science Foundation(31872904)。
文摘Emerging evidence indicated that the increase in food allergy(FA)over the past few decades was associated with the abnormal compositional and metabolic changes of gut microbiota.Gut microbiota played a vital role in maintaining the homeostasis of the immune system and the dysbiosis of gut microbiota promoted the occurrence of FA.Recent research suggested that short-chain fatty acids(SCFAs),the main metabolites derived from gut microbiota,contributed to FA protection.Herein,we provided a comprehensive review on the relationship between gut microbiota and FA.The multifaceted mechanisms underlymg beneficial effects of gut microbiota composition/metabolites on the regulation of diverse cellular pathways in intestinal epithelial cells,dendritic cells,innate lymphoid cells,T cells,B cells and mast cells in the immune system were discussed systematically.These findings emphasized the positive function of gut microbiota in FA and provided novel ideas for the treatment or prevention of FA in the future.
基金supported by the National Natural Science Foundation of China(40930637,41272269)Special Project for Earthquake Science(200808079)+2 种基金Subject Foundation of Ministry of Education for Doctor Candidates in Universities(20100022110001)Fundamental Research Funds for the Central Universities(2652013088)financial support from the China Scholarship Council
文摘Monitoring of subsurface fluid （underground fluid） is an important part of efforts for earthquake prediction in China. The nationwide network, which monitors groundwater level, water temperature, and radon and mercury in groundwater, has been constructed in the last decades. Large amounts of abnormal fluid changes before and after major earthquakes have been recorded, providing precious data for research in earthquake sciences. Many studies have been done in earthquake fluid hydrogeology in order to probe the nature of the earthquake. Much progress in earthquake fluid hydrogeology has been made in the last decades. The paper provides a review of the advances in research on earthquake fluid hydrogeology over the last 40 years in China. It deals with the following five aspects： （1） an introduction to the development history of monitoring networks construction; （2） cases of different subsurface fluid changes recorded before some major earthquakes which occurred in the last decades; （3） characteristics of subsurface fluid changes following major earthquakes; （4） mechanism of subsurface fluid changes before and following earthquakes; （5） application of earthquake fluids in the hydrogeology field.
基金supported by the National Key Research and Development Program of China(2019YFC1605000)the National Natural Science Foundation(31872904)。
文摘With the prevalence of food allergy increasing every year,food allergy has become a common public health problem.More and more studies have shown that probiotics can intervene in food allergy based on the intestinal mucosal immune system.Probiotics and their metabolites can interact with immune cells and gut microbiota to alleviate food allergy.This review outlines the relationship between the intestinal mucosal immune system and food allergy.This review also presents the clinical application and potential immunomodulation mechanisms of probiotics on food allergy.We aim at providing a reference for further studies to explore the key active substances and immunomodulation mechanisms of anti-allergic probiotics.
基金grants from Beijing Hospital Key Research Program(121 Research Program,No.BJ2019-195)。
文摘Objective:Histology grade,subtypes and TNM stage of lung adenocarcinomas are useful predictors of prognosis and survival.The aim of the study was to investigate the relationship between chromosomal instability,morphological subtypes and the grading system used in lung non-mucinous adenocarcinoma(LNMA).Methods:We developed a whole genome copy number variation(WGCNV)scoring system and applied next generation sequencing to evaluate CNVs present in 91 LNMA tumor samples.Results:Higher histological grades,aggressive subtypes and more advanced TNM staging were associated with an increased WGCNV score,particularly in CNV regions enriched for tumor suppressor genes and oncogenes.In addition,we demonstrate that 24-chromosome CNV profiling can be performed reliably from specific cell types(<100 cells)isolated by sample laser capture microdissection.Conclusions:Our findings suggest that the WGCNV scoring system we developed may have potential value as an adjunct test for predicting the prognosis of patients diagnosed with LNMA.
基金supported by the National Key Research and Development Program of China(2019YFC1605000)the National Natural Science Foundation(1872904)。
文摘Increasing incidences showed that food allergies have attracted more and more attention from researchers.BALB/c mice were sensitized with wheat gluten combined with aluminum hydroxide adjuvant via intraperitoneal injection,transdermal sensitization,and oral gavage sensitization route.Results showed that all the three sensitization methods could induce allergic symptoms;increase the serum antibody(total immunoglobulin E(IgE),specific IgE,IgG,IgA)and histamine content;promote the secretion of Th2 cytokines(interleukin(IL)-4,IL-5,IL-13)and inflammatory factors(IL-6,IL-17 A,IL-10);and inhibit the production of Th1 cytokines(IFN-γ,IL-2).However,the allergic symptoms of mice sensitized by intraperitoneal injection were the most obvious among the three models.The level of serum antibodies in intraperitoneal injection group was significantly higher than control.Subsequently,16 S rRNA sequencing technology was used to analyze the intestinal flora of mice.The results showed that the abundance of Firmicutes in the wheat protein sensitized group was lower than that in the normal group,while the abundance of Bacteroidetes was higher,and Lactobacillus was the difference marker in normal group.Bacterial species diversity analysis showed that the species richness and diversity of intestinal flora in mice were decreased,the difference between mice induced by intraperitoneal injection and normal control group mice was the most significant.Taken together,these results show that among three sensitization methods used to build a mouse model with aluminum hydroxide as adjuvant,intraperitoneal injection is the comparably best way to build a mouse sensitization mode.
文摘The conjecture of twin prime numbers is a mathematical problem. Proving the twin prime conjecture using traditional modern number theory is extremely profound and complex. We propose an elementary research method for corresponding prime number, proved that the conjecture of twin prime numbers and obtain the corresponding prime distribution equation. According to the distribution rate of corresponding prime numbers, the distribution pattern of twin prime numbers was proved the distribution rate theorem. This is the distribution rate of prime numbers corresponding to composite numbers, which approaches the distribution rate of prime numbers corresponding to integers. Based on the corresponding prime distribution equation, obtain the twin prime inequality function. Then, the formula for calculating twin prime numbers was discussed. There is also the Hardy Littlewood conjecture. This provides a practical and feasible approach for studying the distribution of twin prime numbers.
基金supported by the National Natural Science Foundation of China(41572238,U1602233)Special Funds for Scientific Research of the Institute of Geology,China Earthquake Administration(IGCEA1205)Special Projects for Seismological Community(200808079).
文摘We have examined the water level data from 16 wells in Sichuan province,China,recorded before the Wenchuan Ms8.0 earthquake occurred in 2008.We found that the data of 5 among these wells exhibit possible precursory anomalies,which are respectively named the Chuan No.08 well in Deyang,Chuan No.ll well in Pujiang,Chuan No.13 well in Luzhou,Chuan No.22 well in Qionglai and Beichuan.In time durations,these anomalies are of long-,intermediate-and short-term signals which are primarily distributed on the northeast(NE)trending Longmenshan fault zone as well as the parallel Huayingshan fault zone.It seems that the variations of the well levels on the Huayingshan fault zone imply compression while those on the Longmenshan fault zone reflect extension.These anomalies occurred first in the areas outside the epicenter region of the Wenchuan Ms8.0 earthquake,then migrated to the seismogenic fault zone.And they migrated from southwest to northeast in agreement with the rupture process of the main shock.
基金supportedby Basic Science Research Special Item of the Institute of Geology, China Earthquake Administration (NoDF-IGCEA-0608-2-10)Special Research Program of China Earthquake Administration (No. 200808079).
文摘We systematically analyze coseismic responses and post-seismic characteristics of groundwater levels in the Three Gorges well-network to the Ms8.0 Wenchuan earthquake on 12 May 2008. The results indicate that these characteristics differ among wells. On the conditions of similar borehole configurations, the differences are associated with geological structural sites of wells, burial types of aquifers monitored, and transmissivities of aquifer systems. We explored coseismic and post-seismic step-rise and step-drop mechanical mechanisms and their implication to earthquake prediction. We validated the inference that the residual step-rise zone is a possible earthquake risk zone based on recent seismic activity on the Xiannüshan fault in the area.
基金The National Natural Science Foundation of China under contract Nos 41906005,41149907 and 41706033the National Basic Research Program of China under contract No.2019-JCJQ-ZD-149-00the Open Fund of the Laboratory for Regional Oceanography and Numerical Modeling,Pilot National Laboratory for Marine Science and Technology(Qingdao)under contract No.2019A05.
文摘Theoretically,propagating internal tides in the ocean may reflect at turning depths,where buoyancy frequencies equal tidal frequencies,before colliding with the air-sea interface or rugged bottom topography.Globally,the internal tide lower turning depths(ITLTDs)in the open ocean have been mapped;however,knowledge of the presence of ITLTDs in the South China Sea(SCS)is lacking.In this study,2125 high-quality temperature-salinity profiles(including 58 deep-sea hydrographic measurements with observational depths exceeding 3000 m)are collected and analyzed to investigate the existence of ITLTDs in the SCS.Furthermore,the concept of the upper turning depth is first introduced in the context of internal tides,and internal tide upper turning depths(ITUTDs)are also investigated.ITLTDs are found to exist at several abyssal stations;these stations are distributed mostly in the southern part of the SCS basin,possibly due to the greater water depths there.Fewer locations show the presence of ITLTDs for K_(1) versus M_(2) tidal frequencies because of the lower tidal frequency.The distance between ITLTDs and the seafloor ranged from 270 m to more than 1200 m,implying the possible existence of multiple internal wave evanescent regions in the abyssal bottom.ITUTDs of tens of meters are ubiquitous in the SCS;stations with the presence of ITUTDs are located mainly in the northeastern SCS due to the intensive observations there.However,the calculated ITUTDs have large uncertainties;they are sensitive to the selected bin values.The horizontal propagation directions of internal tides in the SCS change dramatically,and as a result,the estimated turning depths under the full Coriolis force definition are different compared to that under the traditional approximation.
基金supported by the National Key Research and Development Program of China(2019YFC1605000)National Natural Science Foundation of China(31872904)。
文摘Wheat flour,as the most important source of food globally,is one of the most common causative agents of food allergy.This study aimed to investigate the effects of fermentation on wheat protein digestibility and allergenicity.Protein digestibility were evaluated using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and enzyme-linked immunosorbent assay.The effect of protein on intestinal permeability was investigated by Caco-2 cell monolayers.Co-culture fermentation with Pediococcus acidilactici XZ31 and yeast leads to improvement in digestibility of wheat protein compared to single strain fermentation.Fermentation leads to a decrease in albumin/globulin antigenicity and an increase in gluten R5 reactivity,with the most significant changes in the co-culture group.Digestion strengthen the decrease of protein antigenicity and counteracts the difference in antigenicity induced by fermentation between groups.However,pretreatment with P.acidilactici XZ31 reduces the amount of allergens across Caco-2 monolayer and attenuates the gluten-induced increase in permeability of Caco-2 cell monolayer by reducing actin polymerization and villous atrophy.Co-culture fermentation reduces gluten-induced cell monolayer damage to a greater extent than P.acidilactici XZ31 monoculture.These results gives valuable insight into the effects of P.acidilactici XZ31 fermentation on the allergenicity and toxicity of wheat proteins,which contribute to promoting the application of multi-strain leavening agent in hypoallergenic and gluten-free wheat products.
基金supported by the National Natural Science Foundation of China(Nos.92156012 and 22071078).
文摘Molecular weaving is a powerful approach to make molecularly woven materials that have showed unprecedented characteristics and properties intrinsically distinct to those of non-woven materials.We here report a facile and efficient approach for the synthesis of 2D woven supramolecular polymers by differentiated self-assembly through orthogonal noncovalent interactions.Importantly,the difference in binding strength of two orthogonal noncovalent interactions can be used to control the process of molecular weaving.Consequently,single-layered 2D woven supramolecular polymers were synthesized and fully characterized by various techniques.This study demonstrates a controllable method for molecular weaving,and will significantly hasten the development of molecularly woven materials.
基金the National Natural Science Foundation of China with grants 81872895 and 82073881 awarded to D.Z.,and 81872915,82073904,and 82011530150 to M.-W.W.the Shanghai Municipal Education Commission under the Shanghai Top-Level University Capacity Building Program(DGF817029-04 to M.-W.W.)+3 种基金the Shanghai Science and Technology Commission with grants 18ZR1403900,20430713600,and 18JC1413800 to D.ZInnovative Drug and Evaluation Innovation Team for Tumor Immunotherapy of Jinan Science and Technology Bureau(No.2020GXRC041 to D.Z.)funded by the Fudan School of Pharmacy and Minhang Hospital Joint Research Fund(RO-MY201712 to D.Z.)the Fudan-SIMM Joint Research Fund(FU-SIMM20181010 to D.Z.and D.Y.).
文摘Conventional type 1 dendritic cells(cDC1)are the essential antigen-presenting DC subset in antitumor immunity.Suppressing B-cell lymphoma 9 and B-cell lymphoma 9-like(BCL9/BCL9L)inhibits tumor growth and boosts immune responses against cancer.However,whether oncogenic BCL9/BCL9L impairs antigen presentation in tumors is still not completely understood.Here,we show that targeting BCL9/BCL9L enhanced antigen presentation by stimulating cDC1 activation and infiltration into tumor.Pharmacological inhibition of BCL9/BCL9L with a novel inhibitor hsBCL9z96 or Bcl9/Bcl9l knockout mice markedly delayed tumor growth and promoted antitumor CD8^(+)T cell responses.Mechanistically,targeting BCL9/BCL9L promoted antigen presentation in tumors.This is due to the increase of cDC1 activation and tumor infiltration by the XCL1-XCR1 axis.Importantly,using single-cell transcriptomics analysis,we found that Bcl9/Bcl9l deficient cDC1 were superior to wild-type(WT)cDC1 at activation and antigen presentation via NF-κB/IRF1 signaling.Together,we demonstrate that targeting BCL9/BCL9L plays a crucial role in cDC1-modulated antigen presentation of tumor-derived antigens,as well as CD8^(+)T cell activation and tumor infiltration.Targeting BCL9/BCL9L to regulate cDC1 function and directly orchestrate a positive feedback loop necessary for optimal antitumor immunity could serve as a potential strategy to counter immune suppression and enhance cancer immunotherapy.
基金Supported by National Natural Science Foundation of China(31900271,32160086)Key Technology Research for Identification of Toxic Fungi in Guizhou Province(GKHZC[2020]1Y065)+1 种基金Guizhou Grassroots Science Popularization Action Plan Project([2025]04)Study on Carbon Storage and Carbon Sink Value of Forest Vegetation in State-owned Shatang Forest Farms in Guangxi(2021KY02).
文摘[Objectives] To investigate the species diversity of wood-decaying fungi in karst regions of southwest China. [Methods] The karst core regions of southwest China, specifically Guizhou, Guangxi, and Yunnan, were investigated for the first time to analyze the species diversity characteristics of wood-decaying fungi in this area. This analysis was conducted through a comprehensive 5-year field investigation and systematic identification process. [Results] The wood-decaying fungi exhibited a distinctive species composition and distribution pattern within karst habitats, encompassing significant groups such as Polyporaceae and Hymenochaetaceae. Furthermore, these fungi demonstrated varying functional characteristics across the two ecological types of standing tree decay and wood decay. It was observed that wood-decaying fungi had a significant impact on the material cycle within karst ecosystems by degrading lignin and cellulose. Furthermore, the distribution of species diversity was closely associated with the characteristics of karst geomorphology and the types of vegetation. [Conclusions] This study not only addresses the lack of background data regarding wood-decaying fungi resources in karst regions, but also elucidates the maintenance mechanisms of fragile ecosystems from the perspective of decomposers. It provides a scientific foundation for biodiversity conservation, the prevention and control of forest diseases, and sustainable ecological management in karst regions.
基金supported by the Scientific Research Project of Shanghai Science and Technology Commission of China(No.21DZ1200601)the National Natural Science Foundation of China(No.NSFC52108411).
文摘Vehicle model recognition(VMR)benefits the parking,surveillance,and tolling system by automatically identifying the exact make and model of the passing vehicles.Edge computing technology enables the roadside facilities and mobile cameras to achcieve VMR in realtime.Current work generally relies on a specific view of the vehicle or requires huge calculation capability to deploy the end-to-end deep learning network.This paper proposes a lightweight two-stage identification method based on object detection and image retrieval techniques,which empowers us the ability of recognizing the vehicle model from an arbitrary view.The first-stage model estimates the vehicle posture using object detection and similarity matching,which is cost-efficient and suitable to be programmed in the edge computing devices;the second-stage model retrieves the vehicle’s label from the dataset based on gradient boosting decision tree(GBDT)algorithm and VGGNet,which is flexible to the changing dataset.More than 8000 vehicle images are labeled with their components’information,such as headlights,windows,wheels,and logos.The YOLO network is employed to detect and localize the typical components of a vehicle.The vehicle postures are estimated by the spatial relationship between different segmented components.Due to the variety of the perspectives,a 7-dimensional vector is defined to represent the relative posture of the vehicle and screen out the images with a similar photographic perspective.Two algorithms are used to extract the features from each image patch:(1)the scale invariant feature transform(SIFT)combined with the bag-of-features(BoF)and(2)pre-trained deep neural network.The GBDT is applied to evaluate the weight of each component regarding its impact on VMR.The descriptors of each component are then aggregated to retrieve the best matching image from the database.The results showed its advantages in terms of accuracy(89.2%)and efficiency,demonstrating the vast potential of applying this method to large-scale vehicle model recognition.
基金The authors would like to express their gratitude to the National Natural Science Foundation of China(No.51775286)the Research Grants Council of Hong Kong(Project No.CityU11269216)for the financial support.
文摘Effective oil replenishment to the lubrication track of a running bearing is crucial to its sustainable operation.Reliable practical solutions are rare despite numerous theoretical studies were conducted in the last few decades.This paper proposes the use of surface effect,wettability gradient,to achieve the goal.This method is simple and can be nicely implemented using femtosecond laser ablation.A periodic comb-tooth-shaped pattern with anisotropic wetting capability is devised and its effect on the anisotropic spreading behaviour of an oil droplet is studied.Results show that the comb-tooth-shaped pattern enables the rearrangement of oil distribution,thereby escalating oil replenishment to the lubrication track.The effect is due to the unbalanced interfacial force created by the surface pattern.The influence of the shape and the pitch of teeth,which are the two governing factors,on oil transport is also reported.The effects of the newly devised surface pattern on lubrication are experimentally evaluated under the conditions of limited lubricant supply.These results are promising,demonstrating the reduction in bearing friction and the increase in lubricating film thickness.
基金the National Natural Science Foundation of China(No.51703241)the Fundamental Research Funds for the Central Universities(No.020514380183)+1 种基金the Key Research Program of Frontier Science of Chinese Academy of Sciences(No.QYZDB-SSW-SLH031)the Science and Technology Project of Nanchang(No.2017-SJSYS-008).
文摘Neutral aqueous rechargeable Co_(3)O_(4)//Zn batteries with high-output voltage and outstanding cycling stability have yielded new insights into wearable energy-storage devices.To meet the increasing demand for a means of powering wearable and portable devices,the development of a high-performance fiber-shaped Co//Zn battery would be highly desirable.However,the intrinsically poor conductivity of C 03O4 significantly restricts the application of these high-capacity and high-rate aqueous rechargeable battery.Encouragingly,density functional theory(DFT)calculations demonstrate that the substitution of Zn for Co^(3+)leads to an insulatormetal transition in the Zn-doped Co_(3)O_(4)(Zn-Co_(3)O_(4)).In this study,we used metallic Zn-Co_(3)O_(4)nanowire arrays(NWAs)as a novel binder-free cathode to successfully fabricate an all-solid-state fiber-shaped aqueous rechargeable(AFAR)Co//Zn battery.The resulting fiber-shaped Co//Zn battery takes advantage of the enhanced conductivity,increased capacity,and improved rate capability of Zn-Co_(3)O_(4)NWAs to yield a remarkable capacity of 1.25 mAh·cm^(-2)at a current density of 0.5 mA·cm^(-2),extraordinary rate capability(60.8%capacity retention at a high current density of 20 mA·cm^(-2))and an admirable energy density of 772.6 mWh·cm^(-3).Thus,the successful construction of Zn-Co_(3)O_(4)NWAs provides valuable insights into the design of high-capacity and high-rate cathode materials for aqueous rechargeable high-voltage batteries.
基金This work was supported by the National Natural Science Foundation of China (51972162 and 51703241)the Postdoctoral Foundation of Jiangsu Province (2019Z203 and 2019K001)the Science and Technology Project of Nanchang (2017-SJSYS008)
文摘Fiber-shaped energy storage devices are indispensable parts of wearable and portable electronics.Aqueous rechargeable Ni/Fe battery is a very appropriate energy storage device due to their good safety without organic electrolytes, high ionic conductivity, and low cost. Unfortunately, the low energy density,poor power density and cycling performance hinder its further practical applications. In this study, in order to obtain high performance negative iron-based material, we first synthesized a-iron oxide(α-Fe2O3) nanorods(NRs) with superstructures on the surface of highly conductive carbon nanotube fibers(CNTFs), then electrically conductive polypyrrole(PPy) was coated to enhance the electron, ion diffusion and cycle stability. The as-prepared α-Fe2O3@PPy NRs/CNTF electrode shows a high specific capacity of 0.62 Ah cm-3 at the current density of 1 A cm-3. Furthermore, the Ni/Fe battery that was assembled by the above negative electrode shows a maximum volumetric energy density of 15.47 mWh cm-3 with228.2 mW cm-3 at a current density of 1 A cm-3. The cycling durability and mechanical flexibility of the Ni/Fe battery were tested, which show good prospect for practical application. In summary, these merits make it possible for our Ni/Fe battery to have practical applications in next generation flexible energy storage devices.
