The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of b...The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of black sea bream stock enhancement.In order to detect the contribution of released individuals in Zhujiang River Estuary(ZRE)and Daya Bay(DB),six microsatellite markers were used to identify the hatchery-released individuals.In addition,this pedigree of hatchery populations(broodfish and hatchery-released offspring)was traced to detect the number of effective parents(N_(e)),the inbreeding coefficient and the decrease of genetic variability in the reproduction.The pedigree reconstruction showed that at least 69(out of 93)broodfish had offspring.The estimated N_(e) was 54.8,consequently the inbreeding coefficient was 0.91%.The genetic diversity of hatchery-released offspring was lower than that in that of broodfish(heterozygosity alleles,0.727-0.774),some alleles(number of alleles,61-69)and genetic variance were lost during reproduction.It was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area.A total of 128 hatchery-released black sea bream were identified among 487 recaptured samples in ZRE,while a total of 15 samples were identified among 96 samples in DB.In summary,there was a high survival of released fish.N_(e)vertheless,the results provided evidence to consider a loss of genetic variation in hatcheryreleased stock and a negative genetic effect of the stock enhancement.展开更多
Based on predictable,complementary base pairing,DNA can be artificially pre-designed into versatile DNA nanostructures of well-defined shapes and sizes.With excellent addressability and biocompatibility,DNA nanostruct...Based on predictable,complementary base pairing,DNA can be artificially pre-designed into versatile DNA nanostructures of well-defined shapes and sizes.With excellent addressability and biocompatibility,DNA nanostructures have been widely employed in biomedical research,such as biosensing,bio-imaging,and drug delivery.With the development of the chemical biology of nucleic acid.展开更多
Strontium doped perovskite-type Nd0.7Sr0.3MnO3 ceramics were synthesized completely by high-energy ball milling raw oxides of Nd2O3, SrCO3 and MnO2. The optimal ball milling time and mass ratio of milling balls to raw...Strontium doped perovskite-type Nd0.7Sr0.3MnO3 ceramics were synthesized completely by high-energy ball milling raw oxides of Nd2O3, SrCO3 and MnO2. The optimal ball milling time and mass ratio of milling balls to raw materials are 4 h and 10：1, respectively. The grain size of as-milled Nd0.7Sr0.3MnO3 ceramics ranges from 51 to 93 nm, and the fine particles contain two phases of crystalline phase and amorphous phase. For the Nd0.7r0.3MnO3 synthesized by ball milling and sequent heat treatment, a remarkable colossal electroresistance （CER） effect is observed and the CER ratio reaches 900% at Curie temperature Tc when the load voltage increases from 0.1 to 0.8 V.展开更多
基金The Science and Technology Project of Guangdong Province under contract No.2019B121201001the National Key R&D Program of China under contract No.2019YFD0901301+3 种基金the Fund of China-Vietnam Joint Survey on Fish Stocks in the Common Fishing Zone of the Beibu Gulfthe Central Public-interest Scientific Institution Basal Research Fund,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science under contract No.2021SD14the China-ASEAN Maritime Cooperation Fund(China-Vietnam Fishery Stock Enhancement and Conservation in Beibu Gulf)the Hainan Provincial Natural Science Foundation of China under contract No.320QN358.
文摘The stock enhancement programs for black sea bream Acanthopagrus schlegelii have been conducted in China for a fewyears.However,little information has been reported concerning the effectiveness and genetic effect of black sea bream stock enhancement.In order to detect the contribution of released individuals in Zhujiang River Estuary(ZRE)and Daya Bay(DB),six microsatellite markers were used to identify the hatchery-released individuals.In addition,this pedigree of hatchery populations(broodfish and hatchery-released offspring)was traced to detect the number of effective parents(N_(e)),the inbreeding coefficient and the decrease of genetic variability in the reproduction.The pedigree reconstruction showed that at least 69(out of 93)broodfish had offspring.The estimated N_(e) was 54.8,consequently the inbreeding coefficient was 0.91%.The genetic diversity of hatchery-released offspring was lower than that in that of broodfish(heterozygosity alleles,0.727-0.774),some alleles(number of alleles,61-69)and genetic variance were lost during reproduction.It was observed that wild samples had higher levels of genetic diversity compared with hatchery populations as well as recaptured samples in releasing area.A total of 128 hatchery-released black sea bream were identified among 487 recaptured samples in ZRE,while a total of 15 samples were identified among 96 samples in DB.In summary,there was a high survival of released fish.N_(e)vertheless,the results provided evidence to consider a loss of genetic variation in hatcheryreleased stock and a negative genetic effect of the stock enhancement.
基金This work is supported by the National Key R&D Program of China(2021YFA1200302,2018YFA0208900)the National Natural Science Foundation of China(22025201,22077023,and 21721002)+1 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(XDB36000000)the CAS Interdisciplinary Innovation Team,the Youth Innovation Promotion Association CAS,and K.C.Wong Education Foundation(GJTD-2018-03).
文摘Based on predictable,complementary base pairing,DNA can be artificially pre-designed into versatile DNA nanostructures of well-defined shapes and sizes.With excellent addressability and biocompatibility,DNA nanostructures have been widely employed in biomedical research,such as biosensing,bio-imaging,and drug delivery.With the development of the chemical biology of nucleic acid.
基金the National Natural Science Foundation of China (Grant No.10774040) and the joint Chinese-Russian Project for their financial supports
文摘Strontium doped perovskite-type Nd0.7Sr0.3MnO3 ceramics were synthesized completely by high-energy ball milling raw oxides of Nd2O3, SrCO3 and MnO2. The optimal ball milling time and mass ratio of milling balls to raw materials are 4 h and 10：1, respectively. The grain size of as-milled Nd0.7Sr0.3MnO3 ceramics ranges from 51 to 93 nm, and the fine particles contain two phases of crystalline phase and amorphous phase. For the Nd0.7r0.3MnO3 synthesized by ball milling and sequent heat treatment, a remarkable colossal electroresistance （CER） effect is observed and the CER ratio reaches 900% at Curie temperature Tc when the load voltage increases from 0.1 to 0.8 V.
