Objective: To evaluate the treatment outcome of iron isomaltoside compared with an oral iron supplement in the management of iron deficiency anemia (IDA). Methods: The study included patients with IDA who visited the ...Objective: To evaluate the treatment outcome of iron isomaltoside compared with an oral iron supplement in the management of iron deficiency anemia (IDA). Methods: The study included patients with IDA who visited the Outpatient Clinic of the Department of Hematology, the Affiliated Hospital of Qingdao University from October 2021 to August 2022 and met the inclusion and exclusion criteria. According to the actual application of iron supplementation, the patients were divided into two groups: iron isomaltoside treatment group and oral iron treatment group. Baseline measurements were collected before the start of treatment, and measurements were collected subsequently at intervals of 1 week, 1 month, and 3 months. The hematological parameters analyzed included Hemoglobin (Hb), Mean corpuscular hemoglobin (MCH), Mean Hemoglobin content (MCH), Mean corpuscular Hemoglobin concentration (MCHC), and Platelet (Plt). Safety data and adverse event profiles were recorded. Results: Intra-group comparisons: After 1 month of treatment, the Hb significantly improved (P 0.05). Inter-group comparisons: The biochemical parameters were significantly improved (P 0.05) in the iron isomaltoside group compared with those in the oral iron group after 1 month of iron supplementation in patients with mild and moderate anemia. Adverse reactions were tolerable for the patients in both iron isomaltoside group and oral iron group. Only 1 patient in iron isomaltoside group developed anaphylactic shock during medication and recovered after aggressive rescue. Conclusions: Iron isomaltoside which increases Hb more rapidly compared with the oral iron supplementation has few adverse reactions and good acceptance.展开更多
Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial ...Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation.展开更多
Precise quantifi cation of climate-growth relationships can make a major contribution to scientifi c forest management.However,whether diff erences in the response of growth to climate at diff erent altitudes remains ...Precise quantifi cation of climate-growth relationships can make a major contribution to scientifi c forest management.However,whether diff erences in the response of growth to climate at diff erent altitudes remains unclear.To answer this,264 trees of Larix kaempferi from 88 plots,representing diff erent altitudinal ranges(1000-2100 m)and tree classes were sampled and used to develop tree-ring chronologies.Tree-ring growth(TRG)was either positively(dominant)or negatively(intermediate and suppressed)correlated with climate in diff erent tree classes at diff erent altitudes.TRG was strongly correlated with growing season at low altitudes,but was less sensitive to climate at middle altitudes.It was mainly limited by precipitation and was highly sensitive to climate at low altitudes.Climate-growth relationships at high altitudes were opposite compared to those at low altitudes.TRG of dominant trees was more sensitive to climate change compared to intermediate and suppressed trees.Climate factors(annual temperatures;moisture,the number of frost-free days)had diff erent eff ects on tree-ring growth of diff erent tree classes along altitudinal gradients.It was concluded that the increase in summer temperatures decreased water availability,resulting in a signifi cant decline in growth rates after 2005 at lower altitudes.L.kaempferi is suitable for planting in middle altitudes and dominant trees were the best sampling choice for accurately assessing climate-growth relationships.展开更多
Antimony sulfide–selenide Sb2（S,Se）3,including Sb2S3and Sb2Se3,can be regarded as binary metal chalcogenides semiconductors since Sb2S3and Sb2Se3are isomorphous.They possess abundant elemental storage,nontoxicity,g...Antimony sulfide–selenide Sb2（S,Se）3,including Sb2S3and Sb2Se3,can be regarded as binary metal chalcogenides semiconductors since Sb2S3and Sb2Se3are isomorphous.They possess abundant elemental storage,nontoxicity,good stability with regard to moisture at elevated temperatures and suitable physical parameters for light absorption materials in solar cells.To date,quite a few attempts have been conducted in the materials synthesis,photovoltaic property investigation and device fabrication.Benefiting from previous investigation in thin film solar cells and new generation nanostructured solar cells,this class of materials has been applied in either sensitized-architecture or planar heterojunction solar cells.Decent power conversion efficiencies from 5%to 7.5%have been achieved.Apparently,further improvement on the efficiency is required for future practical applications.To give an overview of this research field,this paper displays some typical researches regarding the methodologies toward the antimony sulfide–selenide synthesis,development of interfacial materials and device fabrications,during which we highlight some critical findings that promote the efficiency enhancement.Finally,this paper proposes some outstanding issue regarding fundamental understanding of the materials,some viewpoints for the efficiency improvement and their future challenges in solar cell applications.展开更多
BACKGROUND： In the process of early screening and interventions to high risk infants with brain damage, the occasion and choosing methods of interventions and the combined application of different interventions are s...BACKGROUND： In the process of early screening and interventions to high risk infants with brain damage, the occasion and choosing methods of interventions and the combined application of different interventions are still at the exploratory phase. OBJECTIVE： To observe the efficacy of early intervention using Vojta and Bobath combined treatment in high risk infants with brain damage, and investigate the effect of early rehabilitation on the prognosis. DESIGN： A randomized controlled comparative observation. SETTING： Daqing Oil Field General Hospital of Heilongjiang Province. PARTICIPANTS： Eighty-four high risk infants younger than l year were selected from the Department of Pediatrics, Daqing Oil Field General Hospital of Heilongjiang Province from October 2005 to October 2006, including 52 boys （62%） and 32 girls （38%）. The treatment started at the age of 0 - 3 months in II cases （13%）, 4 - 6 months in 28 cases （33%）, 7 - 9 months in 35 cases （42%）, and 10 - 12 months in 10 cases （1 2%）. Infants with at least two of the followings were enrolled, including 7 Vojta abnormal postural reflexes, slow or disorder of motor development, increase of muscular tension, postural abnormality, primary reflection residual and CT/MRI abnormalities. Informed consents were obtained from their guardians. The 84 infants were randomly divided into treatment group （n =42） and control group （n =42）. METHODS： All the children were intravenously injected with cerebroprotein hydrolysate injection or cattle encephalon glycoside and ignotin injection, 10 times as a course for 2 - 5 courses; Besides, the infants in the treatment group also received early rehabilitative training of Vojta and Bobath combined treatment, once a day, 40 minutes per time, 5 times a week followed by a 2-day rest, 1 month as a course, and totally 2 - 5 courses. The Vojta method was to facilitate the automatic regulation by reflexlocomotion. Bobath method was to inhibit abnormal posture but facilitate the normal one, thus it is also called neurodevelopmental therapy realized by reflex inhibition and facilitation. The rehabilitation was evaluated clinically using Gesell Development Schedules （GDS） after the courses when the infants were 1 year old. GDS included five aspects of adaptive behavior, gross motor, refine motor, speech, individual-social behavior. The developmental quotient （DQ） could be calculated according to their development. DQ=developmental age/actual age × 100, 〈 85 points was taken as abnormal; The higher the DQ value, the better the development. （2） Evaluation of efficacy： Cured： Developmental order reached normal or generally normal, abnormal posture disappeared, muscular tension ameliorated, symmetric limbs＇ motor function, free movement, normal intelligence, powerful reaction, and clear speech. Significant effect： Developmental order tended to normal, abnormal posture disappeared incompletely, muscular tension ameliorated incompletely, transient pause in the flexion and extension of limbs, good reaction, and improved intelligence. Effective： Developmental order improved to a certain degree, muscular tension ameliorated, limbs＇ motor function and posture improved as compared with before treatment, but still under control, intelligence improved a little. Invalid： No obvious changes before and after treatment. Total significant effective rate=（cured cases＋cases of significant effect）/total cases × 100%. （3） The enumeration data and measurement data were compared with chi-square test and t test respectively. MAIN OUTCOME MEASURES： Clinical efficacy and GDS results at 1 year old in both groups. RESUTLS： All the 84 infants were involved in the analysis of results. （1） Efficacy： The total significant effective rate in the treatment group was higher than that in the control group （93%, 74%, x^2=4.2, P 〈 0.05）. （2） Results of GDS evaluation： The DQ values of individual intercourse and social adaptation in the treatment group （93.57±11.23, 95.13±9.32） were higher than those in the control group （88.27±10.18, 90.10±10.67, t =2.267, 2.301, P 〈 0.05）; The DQ values of gross motor, fine motor and speech in the treatment group （90.78±10.20, 98.63±9.23, 93.69±11.31） were higher than those in the control group （84.32± 11.33, 87.80±8.61, 85.52±10.61, t =2.746, 5.560, 2.746, P 〈 0.01）. The interventional effects on each functional area were significant, especially the gross motor, fine motor and speech. CONCLUSION： The Vojta and Bobath combined treatment on the basis of drug therapy can improve the general developments of abilities in motor, intelligence, social intercourse, social adaptation and speech of high risk infants with brain damage, also can improve the curative efficacy.展开更多
Background: The accurate estimation of soil nutrient content is particularly important in view of its impact on plant growth and forest regeneration. In order to investigate soil nutrient content and quality for the n...Background: The accurate estimation of soil nutrient content is particularly important in view of its impact on plant growth and forest regeneration. In order to investigate soil nutrient content and quality for the natural regeneration of Dacrydium pectinatum communities in China, designing advanced and accurate estimation methods is necessary.Methods: This study uses machine learning techniques created a series of comprehensive and novel models from which to evaluate soil nutrient content. Soil nutrient evaluation methods were built by using six support vector machines and four artificial neural networks.Results: The generalized regression neural network model was the best artificial neural network evaluation model with the smallest root mean square error(5.1), mean error(-0.85), and mean square prediction error(29). The accuracy rate of the combined k-nearest neighbors(k-NN) local support vector machines model(i.e. k-nearest neighbors-support vector machine(KNNSVM)) for soil nutrient evaluation was high, comparing to the other five partial support vector machines models investigated. The area under curve value of generalized regression neural network(0.6572) was the highest, and the cross-validation result showed that the generalized regression neural network reached 92.5%.Conclusions: Both the KNNSVM and generalized regression neural network models can be effectively used to evaluate soil nutrient content and quality grades in conjunction with appropriate model variables. Developing a new feasible evaluation method to assess soil nutrient quality for Dacrydium pectinatum, results from this study can be used as a reference for the adaptive management of rare and endangered tree species. This study, however, found some uncertainties in data acquisition and model simulations, which will be investigated in upcoming studies.展开更多
Leeches and earthworms are the main ingredients of Shuxuetong injection compositions,whichare natural biomedicines.Near infrared(NIR)diffuse reflection spectroscopy has been used forquality assurance of Chinese medici...Leeches and earthworms are the main ingredients of Shuxuetong injection compositions,whichare natural biomedicines.Near infrared(NIR)diffuse reflection spectroscopy has been used forquality assurance of Chinese medicines.In the present work,NIR spectroscopy was proposed as arapid and nondestructive technique to assess the moisture content(MC),soluble solid content(SSC)and hypoxanthine content(HXC)of leeches and earthworms.This study goal was toimprove NIR models for accurate quality control of leech and earthworm using outlier multiplediagnoses(OMD).OMD was composed of four outlier detection methods:spectrum outlier di-agnostic(MD),leverage diagnostic(LD),principal component scores diagnostic(PCSD)andfactor loading diagnostic(FLD),Conventional outlier diagnoses(MD,LD)and OMD werecompared,and the best NIR models were those based on OMD.The correlation coefficients(R)for leech were 0.9779,0.9616 and 0.9406 for MC,SSC and HXC,respectively.The values ofrelative standard error of prediction(RSEP)for leech were 2.3%,5.1%and 9.0%for MC,SSC and HXC,respectively.The values of R for earthworm were 0.9478,0.9991 and 0.9605 for MC,SSC and HXC,respectively.The values of RSEP for earthworm were 8.8%,2.4%and 12%for MC,SSC and HXC,respectively.The performance of the NIR models was certainly improved by OMD.展开更多
Background:Dihydroartemisinin(DHA)is reported to be a potential anticancer agent,and the mechanisms underlying the effects of DHA on diffuse large B cell lymphoma however are still obscure.This study aimed to assess t...Background:Dihydroartemisinin(DHA)is reported to be a potential anticancer agent,and the mechanisms underlying the effects of DHA on diffuse large B cell lymphoma however are still obscure.This study aimed to assess the antitumor effect of DHA on diffuse large B cell lymphoma cells and to determine the potential underlying mechanisms of DHA-induced cell apoptosis.Methods:Here,the Cell Counting Kit 8 assay was conducted to study cell proliferation.We performed Annexin V-FITC/propidium iodide staining,real-time polymerase chain reaction,and western blot analysis to analyze cell apoptosis and potential molecular mechanisms.Results:The results showed that DHA substantially suppressed cell proliferation and induced cell apoptosis in vitro in a time-and concentration-dependent fashion.Moreover,STAT3 activity could be inhibited after stimulation with DHA.Conclusion:These results imply that the underlying anti-tumoral effect of DHA may increase apoptosis in diffuse large B cell lymphoma cells via the STAT3 signaling pathway.In addition,DHA might be an effective drug for diffuse large B cell lymphoma therapy.展开更多
In this study, two rape varieties, ＂Zajiaolvbang＂ and ＂bilv＂, were used as experimental materials to investigate the effect of different lower irrigation limits （80%, 60%, 40% and 20% of field capacity） on quali...In this study, two rape varieties, ＂Zajiaolvbang＂ and ＂bilv＂, were used as experimental materials to investigate the effect of different lower irrigation limits （80%, 60%, 40% and 20% of field capacity） on quality of pet-grown rape. The results showed that contents of soluble sugar, soluble protein, vitamin C, nitrate and organic acids in rape all increased with the decreasing lower irrigation limit. Rape plants represented the best quality with lower irrigation limit of 60% of field capacity.展开更多
[ Objectives] This study was conducted to identify the random amplification of polymorphic DNA （RAPD） markers linked to chewy texture-controlling gene of Chinese cabbage. [ Methods] The RAPD markers associated with ...[ Objectives] This study was conducted to identify the random amplification of polymorphic DNA （RAPD） markers linked to chewy texture-controlling gene of Chinese cabbage. [ Methods] The RAPD markers associated with chewy texture of Chinese cabbage were identified via bulked segregant analysis （BSA） in an F2 population derived from the cross between Hua 273 （female parent） and 114 Fushan （male parent）. [ Results] OPA06-1400 was identified to he linked to the chewy texture-controlling gene of Chinese cabbage. The genetic distance between the target gene and the RAPD marker was 24.8 cM. [ Conclusions] The resuits provide experimental evidence for breeding of Chinese cabbage.展开更多
With the rapid development of social economy,the sense of urgency among enterprises is increasing.The primary reason for this is the fierce competition among enterprises.As an important strategy for establishing a mod...With the rapid development of social economy,the sense of urgency among enterprises is increasing.The primary reason for this is the fierce competition among enterprises.As an important strategy for establishing a modern enterprise system,asset restructuring plays a pivotal role in improving the economic efficiency of enterprises,optimizing the allocation of social resources,and promoting both,social and economic development.Therefore,based on the existing problems of enterprise asset restructuring accounting,this paper discusses the causes of the accounting deficiencies in enterprise asset restructuring from various perspectives,including asset restructuring concept,financial authority and responsibility authority,as well as commercial credit,and subsequently proposes corresponding optimization strategies,which include strengthening asset valuation,enhancing information disclosure of connected transactions,improving relevant accounting standards,as well as strengthening information audit and improving related systems.展开更多
Patients with non-small cell lung cancer(NSCLC)harboring epidermal growth factor receptor(EGFR)mutations show a notable response to tyrosine kinase inhibitor(TKI)treatment.However,almost all patients eventually develo...Patients with non-small cell lung cancer(NSCLC)harboring epidermal growth factor receptor(EGFR)mutations show a notable response to tyrosine kinase inhibitor(TKI)treatment.However,almost all patients eventually develop resistance[1].Given the limited post-resistance treatment options,identifying effective solutions for this sizable group has become a pressing clinical concern.The emergence of immunotherapy has marked a significant breakthrough for patients with NSCLC without driver gene mutations.However,its efficacy remains a subject of debate on the carriers of EGFR mutation.While preclinical studies have hinted at the potential benefits of immunotherapy for patients with EGFR mutations,early clinical investigations have painted a less optimistic picture[2,3].展开更多
Although third-generation Epidermal growth factor receptor—tyrosine kinase inhibitors(EGFR-TKI)is standard of care for patients with EGFR-mutant Non-small cell lung cancer(NSCLC),little is known about the predictors ...Although third-generation Epidermal growth factor receptor—tyrosine kinase inhibitors(EGFR-TKI)is standard of care for patients with EGFR-mutant Non-small cell lung cancer(NSCLC),little is known about the predictors of response or resistance.Here,we integrated single-cell RNA(scRNA)sequencing,bulk RNA sequencing,multiplexed immunofluorescence andflow cytometry data from pretreatment and post-resistant tumor samples of EGFR-mutant NSCLC patients received third-generation EGFR-TKIs.We show that resistant samples had a markedly enriched CXCR1^(+)neutrophils infiltration(P<0.01)than pretreatment samples,which were distinguished from other subtypes of neutrophils and displayed immunosupressive characteristics.Spatial analysis showed that increased CXCR1^(+)neutrophils predominantly infiltrated into the tumor core in resistant samples and the average distance of neutrophils to tumor cells markedly reduced from 33 to 19μm.Deep analysis of scRNA and bulk RNA sequencing data revealed the increased interactions between CXCR1^(+)neutrophils and tumor cells and activated TNF-α/NF-κB signaling pathway in tumor cells of resistant samples.In vitro and in vivo experiments validated that CXCR1^(+)neutrophils resulted in resistance to third-generation EGFR-TKI via activating TNF-α/NF-κB signaling pathway in tumor cells.Importantly,patients with low pretreatment CXCR1^(+)neutrophil infiltration abundance had a dramatically longer progression-free survival(11.8 vs.7.5 months;P=0.019)and overall survival(33.0 vs.23.5 months;P=0.029)than those with high infiltration abundance.Collectively,thesefindings suggest that CXCR1^(+)neutrophils infiltration was associated with the efficacy of third-generation EGFR-TKI in patients with EGFR-mutant NSCLC.展开更多
Stability under light is critical for lead sulfide quantum dots(PbS QDs)in solar cell applications.To improve the stability of PbS QDs solar cells,the influence of the light illumination on the performance of the as-p...Stability under light is critical for lead sulfide quantum dots(PbS QDs)in solar cell applications.To improve the stability of PbS QDs solar cells,the influence of the light illumination on the performance of the as-prepared PbS QDs solar cells was carefully investigated.Combined with X-ray and ultraviolet photoelectron spectroscopies,it was revealed that the 1,2-ethanedithiol(EDT)ligands of the hole transport p-type PbS QDs reacted with the ligands of the PbS QDs active layer under light illumination.The reaction not only undermines the n-type characteristics of the active layer,but also increases the number of defects,leading to a serious deterioration in device performance.An interface layer was introduced to block the EDT penetration to avoid this issue,significantly improving the stability of the device under light irradiation.Moreover,the separation of carriers was also enhanced due to the better matching the energy level of the interface layer.The device with an interface layer yielded a power conversion efficiency of 12.55%and sustained to a long time light illumination.展开更多
N fertilizer altered bacterial community compositions by changing soil nutrients.•Bacterial ammonia oxidation became predominated with the increasing N rate.•Excessive N input caused the information of a more complex ...N fertilizer altered bacterial community compositions by changing soil nutrients.•Bacterial ammonia oxidation became predominated with the increasing N rate.•Excessive N input caused the information of a more complex microbial network.•Intensified microbial competition by excessive N was due to negative link increase.Nitrogen(N)fertilization drives the structure and function of soil microbial communities,which are crucial for regulating soil biogeochemical cycling and maintaining ecosystem stability.Despite the N fertilizer effects on soil microbial composition and diversity have been widely investigated,it is generally overlooked that ecosystem processes are carried out via complex associations among microbiome members.Here,we examined the effects of five N fertilization levels(0,135,180,225,and 360 kg N ha−1)on microbial co-occurrence networks and key functional taxa such as ammonia-oxidizers in paddy soils.The results showed that N addition altered microbial community composition,which were positively related to soil total N and available phosphorus(P)contents.The abundance of ammonia-oxidizing archaea(AOA)significantly decreased after N addition,whereas ammonia-oxidizing bacteria(AOB)increased in N360 treatment.Compared with low-N group(N0 and N135),the high-N group(N225 and N360)shaped more complex microbial webs and thus improved the stability of the microbial community.Partial least squares path modeling further revealed that N fertilizer had a higher effect on microbial network complexity in the high-N group(0.83)than the low-N group(0.49).Although there were more positive links across all microbial networks,the proportion of negative links significantly increased in the high-N network,suggesting that excess N addition aggravated the competition among microbial species.Disentangling these interactions between microbial communities and N fertilization advances our understanding of biogeochemical processes in paddy soils and their effects on nutrient supply to rice production.Our findings highlighted that highly N-enriched paddy soils have more stable microbial networks and can better sustain soil ecological functions to cope with the ongoing environmental changes.展开更多
The gut microbiota,serving as the only“organ”that humans can share,has been extensively applied in the treatment of various intestinal and extraintestinal diseases through fecal microbiota transplantation(FMT)techno...The gut microbiota,serving as the only“organ”that humans can share,has been extensively applied in the treatment of various intestinal and extraintestinal diseases through fecal microbiota transplantation(FMT)technology.By optimizing the donor selection strategy,FMT exhibited the highest effectiveness(more than 90%)in treating acute intestinal inflammations,such as recurrent Clostridium difficile infection.However,the efficacy diminishes when addressing intestinal functional diseases(more than 60%),chronic intestinal inflammations or organic diseases(less than 60%),and extraintestinal diseases,particularly those lacking any gastrointestinal symptoms(less than 50%).Ongoing efforts to enhance the therapeutic response of FMT across diverse diseases represent a central focus of current clinical research.This review concentrates on donor selection,recipient management,precise donor-recipient matching,antagonistic mechanism of native bacteria to transplanted bacteria,and targeted treatment strategies for different diseases,with the aim of providing practical strategies to improve the clinical efficacy of FMT.展开更多
In vivo imaging of large-scale neuronal activity plays a pivotal role in unraveling the function of the brain’s circuitry.Multiphoton microscopy,a powerful tool for deep-tissue imaging,has received sustained interest...In vivo imaging of large-scale neuronal activity plays a pivotal role in unraveling the function of the brain’s circuitry.Multiphoton microscopy,a powerful tool for deep-tissue imaging,has received sustained interest in advancing its speed,field of view and imaging depth.However,to avoid thermal damage in scattering biological tissue,field of view decreases exponentially as imaging depth increases.We present a suite of innovations to optimize three-photon microscopy for large field-of-view imaging at depths unreachable by two-photon microscopy.These techniques enable us to image neuronal activities of transgenic animals expressing protein calcium sensors in a~3.5-mm diameter field-of-view with single-cell resolution in the deepest cortical layer of mouse brains.We further demonstrate simultaneous large field-of-view two-photon and three-photon imaging,subcortical imaging in the mouse brain,and whole-brain imaging in adult zebrafish.The demonstrated techniques can be integrated into typical multiphoton microscopes to enlarge field of view for system-level neural circuit research.展开更多
Solar-blind deep-ultraviolet(DUV)photodetectors based on Ga_(2)O_(3)have attracted great attention due to their potential applications for many military and civil purposes.However,the development of device integration...Solar-blind deep-ultraviolet(DUV)photodetectors based on Ga_(2)O_(3)have attracted great attention due to their potential applications for many military and civil purposes.However,the development of device integration for optoelectronic system applications remains a huge challenge.Herein,we report a facile method for patterned-growth of high-qualityβ-Ga_(2)O_(3)thin films,which are assembled into a photodetectors array comprising 8×8 device units.A representative detector exhibits outstanding photoresponse performance,in terms of an ultra-low dark current of 0.62 pA,a large Ilight/Idark ratio exceeding 10^(4),a high responsivity of 0.72 A W^(-1) and a decent specific detectivity of 4.18×10^(11)Jones,upon 265 nm DUV illumination.What is more,the DUV/visible(250/400 nm)rejection ratio is as high as 10^(3) with a sharp response cut-off wave length at 280 nm.Further optoelectronic analysis reveals that the photodetectors array has good uniformity and repeatability,endowing it the capability to serve as a reliable DUV light image sensor with a decent spatial resolution.These results suggest that the proposed technique offers an effective avenue for patterned growth ofβ-Ga_(2)O_(3)thin films for multifunctional DUV optoelectronic applications.展开更多
Sb_2S_3 is a kind of stable light absorption materials with suitable band gap, promising for practical applications. Here we demonstrate that the engineering on the composition ratio enables significant improvement in...Sb_2S_3 is a kind of stable light absorption materials with suitable band gap, promising for practical applications. Here we demonstrate that the engineering on the composition ratio enables significant improvement in the device performance. We found that the co-evaporation of sulfur or antimony with Sb_2S_3 is able to generate sulfur-or antimony-rich Sb_2S_3. This composition does not generate essential influence on the crystal structure, optical band and film formability, while the carrier concentration and transport dynamics are considerably changed. The device investigations show that sulfur-rich Sb_2S_3 film is favorable for efficient energy conversion, while antimony-rich Sb_2S_3 leads to greatly decreased device performance. With optimizations on the sulfur-rich Sb_2S_3 films, the final power conversion efficiency reaches5.8%, which is the highest efficiency in thermal evaporation derived Sb_2S_3 solar cells.展开更多
Exposure of airborne particulate matter(PM)with an aerodynamic diameter less than 2.5μm(PM2.5)is epidemiologically associated with lung dysfunction and respiratory symptoms,including pulmonary fibrosis.However,whethe...Exposure of airborne particulate matter(PM)with an aerodynamic diameter less than 2.5μm(PM2.5)is epidemiologically associated with lung dysfunction and respiratory symptoms,including pulmonary fibrosis.However,whether epigenetic mechanisms are involved in PM2.5-induced pulmonary fibrosis is currently poorly understood.Herein,using a PM2.5-induced pulmonary fibrosis mouse model,we found that PM2.5 exposure leads to aberrant mRNA5-methylcytosine(m5C)gain and loss in fibrotic lung tissues.Moreover,we showed the m5C-mediated regulatory map of gene functions in pulmonary fibrosis after PM2.5 exposure.Several genes act as m5C gain-upregulated factors,probably critical for the development of PM2.5-induced fibrosis in mouse lungs.These genes,including Lcn2,Mmp9,Chi3l1,Adipoq,Atp5j2,Atp5l,Atpif1,Ndufb6,Fgr,Slc11 a1,and Tyrobp,are highly related to oxidative stress response,inflammatory responses,and immune system processes.Our study illustrates the first epitranscriptomic RNA m5C profile in PM2.5-induced pulmonary fibrosis and will be valuable in identifying biomarkers for PM2.5 exposure-related lung pathogenesis with translational potential.展开更多
文摘Objective: To evaluate the treatment outcome of iron isomaltoside compared with an oral iron supplement in the management of iron deficiency anemia (IDA). Methods: The study included patients with IDA who visited the Outpatient Clinic of the Department of Hematology, the Affiliated Hospital of Qingdao University from October 2021 to August 2022 and met the inclusion and exclusion criteria. According to the actual application of iron supplementation, the patients were divided into two groups: iron isomaltoside treatment group and oral iron treatment group. Baseline measurements were collected before the start of treatment, and measurements were collected subsequently at intervals of 1 week, 1 month, and 3 months. The hematological parameters analyzed included Hemoglobin (Hb), Mean corpuscular hemoglobin (MCH), Mean Hemoglobin content (MCH), Mean corpuscular Hemoglobin concentration (MCHC), and Platelet (Plt). Safety data and adverse event profiles were recorded. Results: Intra-group comparisons: After 1 month of treatment, the Hb significantly improved (P 0.05). Inter-group comparisons: The biochemical parameters were significantly improved (P 0.05) in the iron isomaltoside group compared with those in the oral iron group after 1 month of iron supplementation in patients with mild and moderate anemia. Adverse reactions were tolerable for the patients in both iron isomaltoside group and oral iron group. Only 1 patient in iron isomaltoside group developed anaphylactic shock during medication and recovered after aggressive rescue. Conclusions: Iron isomaltoside which increases Hb more rapidly compared with the oral iron supplementation has few adverse reactions and good acceptance.
基金supported by grants from Guangdong Provincial Key Lab of Translational Medicine in Lung Cancer (Grant No. 2017B030314120)General Research Project of Guangzhou Science and Technology Bureau (Grant No. 201607010391)+1 种基金National Key Research and Development Program of China (Grant No. 2016YFC1303800)Guangdong Provincial Applied S&T R&D Program (Grant No. 2016B020237006)
文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation.
基金funded by Fundamental Research Funds of CAF (CAFYBB2022ZA00103)National Natural Science Foundation of China (General Program)(31971652)+1 种基金National Natural Science Foundation of China (32001308)Fundamental Research Funds of CAF (CAFYBB2022ZC001)
文摘Precise quantifi cation of climate-growth relationships can make a major contribution to scientifi c forest management.However,whether diff erences in the response of growth to climate at diff erent altitudes remains unclear.To answer this,264 trees of Larix kaempferi from 88 plots,representing diff erent altitudinal ranges(1000-2100 m)and tree classes were sampled and used to develop tree-ring chronologies.Tree-ring growth(TRG)was either positively(dominant)or negatively(intermediate and suppressed)correlated with climate in diff erent tree classes at diff erent altitudes.TRG was strongly correlated with growing season at low altitudes,but was less sensitive to climate at middle altitudes.It was mainly limited by precipitation and was highly sensitive to climate at low altitudes.Climate-growth relationships at high altitudes were opposite compared to those at low altitudes.TRG of dominant trees was more sensitive to climate change compared to intermediate and suppressed trees.Climate factors(annual temperatures;moisture,the number of frost-free days)had diff erent eff ects on tree-ring growth of diff erent tree classes along altitudinal gradients.It was concluded that the increase in summer temperatures decreased water availability,resulting in a signifi cant decline in growth rates after 2005 at lower altitudes.L.kaempferi is suitable for planting in middle altitudes and dominant trees were the best sampling choice for accurately assessing climate-growth relationships.
基金supported by the Recruitment Program of Global Expertsthe Fundamental Research Funds for the Central Universities(Nos.WK2060140022,WK2060140023 and WK2060140024)
文摘Antimony sulfide–selenide Sb2（S,Se）3,including Sb2S3and Sb2Se3,can be regarded as binary metal chalcogenides semiconductors since Sb2S3and Sb2Se3are isomorphous.They possess abundant elemental storage,nontoxicity,good stability with regard to moisture at elevated temperatures and suitable physical parameters for light absorption materials in solar cells.To date,quite a few attempts have been conducted in the materials synthesis,photovoltaic property investigation and device fabrication.Benefiting from previous investigation in thin film solar cells and new generation nanostructured solar cells,this class of materials has been applied in either sensitized-architecture or planar heterojunction solar cells.Decent power conversion efficiencies from 5%to 7.5%have been achieved.Apparently,further improvement on the efficiency is required for future practical applications.To give an overview of this research field,this paper displays some typical researches regarding the methodologies toward the antimony sulfide–selenide synthesis,development of interfacial materials and device fabrications,during which we highlight some critical findings that promote the efficiency enhancement.Finally,this paper proposes some outstanding issue regarding fundamental understanding of the materials,some viewpoints for the efficiency improvement and their future challenges in solar cell applications.
文摘BACKGROUND： In the process of early screening and interventions to high risk infants with brain damage, the occasion and choosing methods of interventions and the combined application of different interventions are still at the exploratory phase. OBJECTIVE： To observe the efficacy of early intervention using Vojta and Bobath combined treatment in high risk infants with brain damage, and investigate the effect of early rehabilitation on the prognosis. DESIGN： A randomized controlled comparative observation. SETTING： Daqing Oil Field General Hospital of Heilongjiang Province. PARTICIPANTS： Eighty-four high risk infants younger than l year were selected from the Department of Pediatrics, Daqing Oil Field General Hospital of Heilongjiang Province from October 2005 to October 2006, including 52 boys （62%） and 32 girls （38%）. The treatment started at the age of 0 - 3 months in II cases （13%）, 4 - 6 months in 28 cases （33%）, 7 - 9 months in 35 cases （42%）, and 10 - 12 months in 10 cases （1 2%）. Infants with at least two of the followings were enrolled, including 7 Vojta abnormal postural reflexes, slow or disorder of motor development, increase of muscular tension, postural abnormality, primary reflection residual and CT/MRI abnormalities. Informed consents were obtained from their guardians. The 84 infants were randomly divided into treatment group （n =42） and control group （n =42）. METHODS： All the children were intravenously injected with cerebroprotein hydrolysate injection or cattle encephalon glycoside and ignotin injection, 10 times as a course for 2 - 5 courses; Besides, the infants in the treatment group also received early rehabilitative training of Vojta and Bobath combined treatment, once a day, 40 minutes per time, 5 times a week followed by a 2-day rest, 1 month as a course, and totally 2 - 5 courses. The Vojta method was to facilitate the automatic regulation by reflexlocomotion. Bobath method was to inhibit abnormal posture but facilitate the normal one, thus it is also called neurodevelopmental therapy realized by reflex inhibition and facilitation. The rehabilitation was evaluated clinically using Gesell Development Schedules （GDS） after the courses when the infants were 1 year old. GDS included five aspects of adaptive behavior, gross motor, refine motor, speech, individual-social behavior. The developmental quotient （DQ） could be calculated according to their development. DQ=developmental age/actual age × 100, 〈 85 points was taken as abnormal; The higher the DQ value, the better the development. （2） Evaluation of efficacy： Cured： Developmental order reached normal or generally normal, abnormal posture disappeared, muscular tension ameliorated, symmetric limbs＇ motor function, free movement, normal intelligence, powerful reaction, and clear speech. Significant effect： Developmental order tended to normal, abnormal posture disappeared incompletely, muscular tension ameliorated incompletely, transient pause in the flexion and extension of limbs, good reaction, and improved intelligence. Effective： Developmental order improved to a certain degree, muscular tension ameliorated, limbs＇ motor function and posture improved as compared with before treatment, but still under control, intelligence improved a little. Invalid： No obvious changes before and after treatment. Total significant effective rate=（cured cases＋cases of significant effect）/total cases × 100%. （3） The enumeration data and measurement data were compared with chi-square test and t test respectively. MAIN OUTCOME MEASURES： Clinical efficacy and GDS results at 1 year old in both groups. RESUTLS： All the 84 infants were involved in the analysis of results. （1） Efficacy： The total significant effective rate in the treatment group was higher than that in the control group （93%, 74%, x^2=4.2, P 〈 0.05）. （2） Results of GDS evaluation： The DQ values of individual intercourse and social adaptation in the treatment group （93.57±11.23, 95.13±9.32） were higher than those in the control group （88.27±10.18, 90.10±10.67, t =2.267, 2.301, P 〈 0.05）; The DQ values of gross motor, fine motor and speech in the treatment group （90.78±10.20, 98.63±9.23, 93.69±11.31） were higher than those in the control group （84.32± 11.33, 87.80±8.61, 85.52±10.61, t =2.746, 5.560, 2.746, P 〈 0.01）. The interventional effects on each functional area were significant, especially the gross motor, fine motor and speech. CONCLUSION： The Vojta and Bobath combined treatment on the basis of drug therapy can improve the general developments of abilities in motor, intelligence, social intercourse, social adaptation and speech of high risk infants with brain damage, also can improve the curative efficacy.
基金financially supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFBB2017ZB004)。
文摘Background: The accurate estimation of soil nutrient content is particularly important in view of its impact on plant growth and forest regeneration. In order to investigate soil nutrient content and quality for the natural regeneration of Dacrydium pectinatum communities in China, designing advanced and accurate estimation methods is necessary.Methods: This study uses machine learning techniques created a series of comprehensive and novel models from which to evaluate soil nutrient content. Soil nutrient evaluation methods were built by using six support vector machines and four artificial neural networks.Results: The generalized regression neural network model was the best artificial neural network evaluation model with the smallest root mean square error(5.1), mean error(-0.85), and mean square prediction error(29). The accuracy rate of the combined k-nearest neighbors(k-NN) local support vector machines model(i.e. k-nearest neighbors-support vector machine(KNNSVM)) for soil nutrient evaluation was high, comparing to the other five partial support vector machines models investigated. The area under curve value of generalized regression neural network(0.6572) was the highest, and the cross-validation result showed that the generalized regression neural network reached 92.5%.Conclusions: Both the KNNSVM and generalized regression neural network models can be effectively used to evaluate soil nutrient content and quality grades in conjunction with appropriate model variables. Developing a new feasible evaluation method to assess soil nutrient quality for Dacrydium pectinatum, results from this study can be used as a reference for the adaptive management of rare and endangered tree species. This study, however, found some uncertainties in data acquisition and model simulations, which will be investigated in upcoming studies.
文摘Leeches and earthworms are the main ingredients of Shuxuetong injection compositions,whichare natural biomedicines.Near infrared(NIR)diffuse reflection spectroscopy has been used forquality assurance of Chinese medicines.In the present work,NIR spectroscopy was proposed as arapid and nondestructive technique to assess the moisture content(MC),soluble solid content(SSC)and hypoxanthine content(HXC)of leeches and earthworms.This study goal was toimprove NIR models for accurate quality control of leech and earthworm using outlier multiplediagnoses(OMD).OMD was composed of four outlier detection methods:spectrum outlier di-agnostic(MD),leverage diagnostic(LD),principal component scores diagnostic(PCSD)andfactor loading diagnostic(FLD),Conventional outlier diagnoses(MD,LD)and OMD werecompared,and the best NIR models were those based on OMD.The correlation coefficients(R)for leech were 0.9779,0.9616 and 0.9406 for MC,SSC and HXC,respectively.The values ofrelative standard error of prediction(RSEP)for leech were 2.3%,5.1%and 9.0%for MC,SSC and HXC,respectively.The values of R for earthworm were 0.9478,0.9991 and 0.9605 for MC,SSC and HXC,respectively.The values of RSEP for earthworm were 8.8%,2.4%and 12%for MC,SSC and HXC,respectively.The performance of the NIR models was certainly improved by OMD.
基金supported by the Shandong Provincial Natural Science Foundation of China(ZR2019MH096).
文摘Background:Dihydroartemisinin(DHA)is reported to be a potential anticancer agent,and the mechanisms underlying the effects of DHA on diffuse large B cell lymphoma however are still obscure.This study aimed to assess the antitumor effect of DHA on diffuse large B cell lymphoma cells and to determine the potential underlying mechanisms of DHA-induced cell apoptosis.Methods:Here,the Cell Counting Kit 8 assay was conducted to study cell proliferation.We performed Annexin V-FITC/propidium iodide staining,real-time polymerase chain reaction,and western blot analysis to analyze cell apoptosis and potential molecular mechanisms.Results:The results showed that DHA substantially suppressed cell proliferation and induced cell apoptosis in vitro in a time-and concentration-dependent fashion.Moreover,STAT3 activity could be inhibited after stimulation with DHA.Conclusion:These results imply that the underlying anti-tumoral effect of DHA may increase apoptosis in diffuse large B cell lymphoma cells via the STAT3 signaling pathway.In addition,DHA might be an effective drug for diffuse large B cell lymphoma therapy.
基金Supported by Project of Modern Agricultural Industry Technology System of Jilin Province-Research and Demonstration of High-efficiency Production Technology for Protected VegetablesSpecial Fund for Breeding of Jilin Province Department of Finance(201104)
文摘In this study, two rape varieties, ＂Zajiaolvbang＂ and ＂bilv＂, were used as experimental materials to investigate the effect of different lower irrigation limits （80%, 60%, 40% and 20% of field capacity） on quality of pet-grown rape. The results showed that contents of soluble sugar, soluble protein, vitamin C, nitrate and organic acids in rape all increased with the decreasing lower irrigation limit. Rape plants represented the best quality with lower irrigation limit of 60% of field capacity.
文摘[ Objectives] This study was conducted to identify the random amplification of polymorphic DNA （RAPD） markers linked to chewy texture-controlling gene of Chinese cabbage. [ Methods] The RAPD markers associated with chewy texture of Chinese cabbage were identified via bulked segregant analysis （BSA） in an F2 population derived from the cross between Hua 273 （female parent） and 114 Fushan （male parent）. [ Results] OPA06-1400 was identified to he linked to the chewy texture-controlling gene of Chinese cabbage. The genetic distance between the target gene and the RAPD marker was 24.8 cM. [ Conclusions] The resuits provide experimental evidence for breeding of Chinese cabbage.
文摘With the rapid development of social economy,the sense of urgency among enterprises is increasing.The primary reason for this is the fierce competition among enterprises.As an important strategy for establishing a modern enterprise system,asset restructuring plays a pivotal role in improving the economic efficiency of enterprises,optimizing the allocation of social resources,and promoting both,social and economic development.Therefore,based on the existing problems of enterprise asset restructuring accounting,this paper discusses the causes of the accounting deficiencies in enterprise asset restructuring from various perspectives,including asset restructuring concept,financial authority and responsibility authority,as well as commercial credit,and subsequently proposes corresponding optimization strategies,which include strengthening asset valuation,enhancing information disclosure of connected transactions,improving relevant accounting standards,as well as strengthening information audit and improving related systems.
基金supported by the China Postdoctoral Science Foundation(2023M742119)the National Natural Science Foundation of China(82403395,82373319,and 82102766)+2 种基金the Natural Science Foundation of Shandong Province(ZR2024QH219)the National Key Clinical Program on Oncologythe Shanghai Science and Technology Innovation Action Plan Medical Innovation Research Special Project(23Y11908600)。
文摘Patients with non-small cell lung cancer(NSCLC)harboring epidermal growth factor receptor(EGFR)mutations show a notable response to tyrosine kinase inhibitor(TKI)treatment.However,almost all patients eventually develop resistance[1].Given the limited post-resistance treatment options,identifying effective solutions for this sizable group has become a pressing clinical concern.The emergence of immunotherapy has marked a significant breakthrough for patients with NSCLC without driver gene mutations.However,its efficacy remains a subject of debate on the carriers of EGFR mutation.While preclinical studies have hinted at the potential benefits of immunotherapy for patients with EGFR mutations,early clinical investigations have painted a less optimistic picture[2,3].
基金supported in part by grants from the National Natural Science Foundation of China(Nos.82102859,82272875 and 12126605)Shanghai Rising-Star Program(Nos.23QA1408000 and 22QA1407800)+5 种基金Shanghai Science and Technology Innovation Action Plan Medical Innovation Research Program(Nos.23Y11904100 and 21Y11913600)the Health and Family Planning Commission of Shanghai Municipality(No.20224Y0067)Ningbo Top Medical and Health Research Program(No.2022030208)Shanghai Nature Foundation Project(No.21ZR1453200)National Key Clinical Specialty Construction Project,Original exploration project of Shanghai Natural Science Foundation(No.23ZR1480600)Shanghai“Rising Stars of Medical Talent”Youth Development Program Youth Medical Talents-Specialist Program.
文摘Although third-generation Epidermal growth factor receptor—tyrosine kinase inhibitors(EGFR-TKI)is standard of care for patients with EGFR-mutant Non-small cell lung cancer(NSCLC),little is known about the predictors of response or resistance.Here,we integrated single-cell RNA(scRNA)sequencing,bulk RNA sequencing,multiplexed immunofluorescence andflow cytometry data from pretreatment and post-resistant tumor samples of EGFR-mutant NSCLC patients received third-generation EGFR-TKIs.We show that resistant samples had a markedly enriched CXCR1^(+)neutrophils infiltration(P<0.01)than pretreatment samples,which were distinguished from other subtypes of neutrophils and displayed immunosupressive characteristics.Spatial analysis showed that increased CXCR1^(+)neutrophils predominantly infiltrated into the tumor core in resistant samples and the average distance of neutrophils to tumor cells markedly reduced from 33 to 19μm.Deep analysis of scRNA and bulk RNA sequencing data revealed the increased interactions between CXCR1^(+)neutrophils and tumor cells and activated TNF-α/NF-κB signaling pathway in tumor cells of resistant samples.In vitro and in vivo experiments validated that CXCR1^(+)neutrophils resulted in resistance to third-generation EGFR-TKI via activating TNF-α/NF-κB signaling pathway in tumor cells.Importantly,patients with low pretreatment CXCR1^(+)neutrophil infiltration abundance had a dramatically longer progression-free survival(11.8 vs.7.5 months;P=0.019)and overall survival(33.0 vs.23.5 months;P=0.029)than those with high infiltration abundance.Collectively,thesefindings suggest that CXCR1^(+)neutrophils infiltration was associated with the efficacy of third-generation EGFR-TKI in patients with EGFR-mutant NSCLC.
基金supported by the National Key Research and Development Program of China(No.2022YFB3602902)Zhejiang Provincial Natural Science Foundation of China(No.LR21F050001)+5 种基金YONGJIANG Talent Introduction Program(No.2021A-038-B)Ningbo 3315 Program(No.2020A01-B)Zhejiang Innovation and Entrepreneurship Team(No.2021R01003)Science and Technology Innovation 2025 Major Project of Ningbo(No.2022Z085)supported by the Flexible Electronics Zhejiang Province Key Laboratory fund project(No.2022FE002)and the Key projects of National Natural Science Foundation of China(No.62234004)dedicated in memory of Dr.Lei Qian,who was a great mentor,colleague,and friend.He made many significant scientific contributions during his highly productive career and will be remembered.
文摘Stability under light is critical for lead sulfide quantum dots(PbS QDs)in solar cell applications.To improve the stability of PbS QDs solar cells,the influence of the light illumination on the performance of the as-prepared PbS QDs solar cells was carefully investigated.Combined with X-ray and ultraviolet photoelectron spectroscopies,it was revealed that the 1,2-ethanedithiol(EDT)ligands of the hole transport p-type PbS QDs reacted with the ligands of the PbS QDs active layer under light illumination.The reaction not only undermines the n-type characteristics of the active layer,but also increases the number of defects,leading to a serious deterioration in device performance.An interface layer was introduced to block the EDT penetration to avoid this issue,significantly improving the stability of the device under light irradiation.Moreover,the separation of carriers was also enhanced due to the better matching the energy level of the interface layer.The device with an interface layer yielded a power conversion efficiency of 12.55%and sustained to a long time light illumination.
基金financially supported by the Joint Funds of the National Natural Science of China(U21A20237).
文摘N fertilizer altered bacterial community compositions by changing soil nutrients.•Bacterial ammonia oxidation became predominated with the increasing N rate.•Excessive N input caused the information of a more complex microbial network.•Intensified microbial competition by excessive N was due to negative link increase.Nitrogen(N)fertilization drives the structure and function of soil microbial communities,which are crucial for regulating soil biogeochemical cycling and maintaining ecosystem stability.Despite the N fertilizer effects on soil microbial composition and diversity have been widely investigated,it is generally overlooked that ecosystem processes are carried out via complex associations among microbiome members.Here,we examined the effects of five N fertilization levels(0,135,180,225,and 360 kg N ha−1)on microbial co-occurrence networks and key functional taxa such as ammonia-oxidizers in paddy soils.The results showed that N addition altered microbial community composition,which were positively related to soil total N and available phosphorus(P)contents.The abundance of ammonia-oxidizing archaea(AOA)significantly decreased after N addition,whereas ammonia-oxidizing bacteria(AOB)increased in N360 treatment.Compared with low-N group(N0 and N135),the high-N group(N225 and N360)shaped more complex microbial webs and thus improved the stability of the microbial community.Partial least squares path modeling further revealed that N fertilizer had a higher effect on microbial network complexity in the high-N group(0.83)than the low-N group(0.49).Although there were more positive links across all microbial networks,the proportion of negative links significantly increased in the high-N network,suggesting that excess N addition aggravated the competition among microbial species.Disentangling these interactions between microbial communities and N fertilization advances our understanding of biogeochemical processes in paddy soils and their effects on nutrient supply to rice production.Our findings highlighted that highly N-enriched paddy soils have more stable microbial networks and can better sustain soil ecological functions to cope with the ongoing environmental changes.
基金This study was supported by the National NaturalScience Foundation of China(81972221,81730102,and 82300753)China postdoctoral science foundation(2022M722412)+6 种基金Top priority project of Shanghai(076478684Q/2023-00154)Major project of Zhangjiang(ZJ2022-ZD-005)Clinical Research Plan of SHDC(SHDC2020CR4026,SHDC2020CR1030B,and SHDC2022CRS041)Project of Shanghai Science and Technology Commission(YDZX20213100003690)Project of Shanghai Municipal Health Commission(19YF1431000 and ZHYY-ZXYJHZX-202105)Anhui Provincial Key R&D Programmes(202304295107020025)Climb Plan of Tenth People’s Hospital of Tongji University(2021SYPDRC045).
文摘The gut microbiota,serving as the only“organ”that humans can share,has been extensively applied in the treatment of various intestinal and extraintestinal diseases through fecal microbiota transplantation(FMT)technology.By optimizing the donor selection strategy,FMT exhibited the highest effectiveness(more than 90%)in treating acute intestinal inflammations,such as recurrent Clostridium difficile infection.However,the efficacy diminishes when addressing intestinal functional diseases(more than 60%),chronic intestinal inflammations or organic diseases(less than 60%),and extraintestinal diseases,particularly those lacking any gastrointestinal symptoms(less than 50%).Ongoing efforts to enhance the therapeutic response of FMT across diverse diseases represent a central focus of current clinical research.This review concentrates on donor selection,recipient management,precise donor-recipient matching,antagonistic mechanism of native bacteria to transplanted bacteria,and targeted treatment strategies for different diseases,with the aim of providing practical strategies to improve the clinical efficacy of FMT.
基金National Science Foundation NeuroNex(Grant No.DBI-1707312 to C.X.).NIH/NINDS(Grant No.U01NS103516 to C.X.).Cornell Neurotech Mong Fellowship to A.M.
文摘In vivo imaging of large-scale neuronal activity plays a pivotal role in unraveling the function of the brain’s circuitry.Multiphoton microscopy,a powerful tool for deep-tissue imaging,has received sustained interest in advancing its speed,field of view and imaging depth.However,to avoid thermal damage in scattering biological tissue,field of view decreases exponentially as imaging depth increases.We present a suite of innovations to optimize three-photon microscopy for large field-of-view imaging at depths unreachable by two-photon microscopy.These techniques enable us to image neuronal activities of transgenic animals expressing protein calcium sensors in a~3.5-mm diameter field-of-view with single-cell resolution in the deepest cortical layer of mouse brains.We further demonstrate simultaneous large field-of-view two-photon and three-photon imaging,subcortical imaging in the mouse brain,and whole-brain imaging in adult zebrafish.The demonstrated techniques can be integrated into typical multiphoton microscopes to enlarge field of view for system-level neural circuit research.
基金supported by the National Natural Science Foundation of China(NSFC,Nos.51902078,62074048)the Fundamental Research Funds for the Central Universities(PA2020GDKC0014,JZ2020HGTB0051,JZ2018HGXC0001)the Anhui Provincial Natural Science Foundation(2008085MF205)。
文摘Solar-blind deep-ultraviolet(DUV)photodetectors based on Ga_(2)O_(3)have attracted great attention due to their potential applications for many military and civil purposes.However,the development of device integration for optoelectronic system applications remains a huge challenge.Herein,we report a facile method for patterned-growth of high-qualityβ-Ga_(2)O_(3)thin films,which are assembled into a photodetectors array comprising 8×8 device units.A representative detector exhibits outstanding photoresponse performance,in terms of an ultra-low dark current of 0.62 pA,a large Ilight/Idark ratio exceeding 10^(4),a high responsivity of 0.72 A W^(-1) and a decent specific detectivity of 4.18×10^(11)Jones,upon 265 nm DUV illumination.What is more,the DUV/visible(250/400 nm)rejection ratio is as high as 10^(3) with a sharp response cut-off wave length at 280 nm.Further optoelectronic analysis reveals that the photodetectors array has good uniformity and repeatability,endowing it the capability to serve as a reliable DUV light image sensor with a decent spatial resolution.These results suggest that the proposed technique offers an effective avenue for patterned growth ofβ-Ga_(2)O_(3)thin films for multifunctional DUV optoelectronic applications.
基金supported by the Fundamental Research Funds for the Central Universities(WK2060140023,CX3430000001,and WK2060140024)the Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology(2016FXZY003)the National Natural Science Foundation of China(U1732150)
文摘Sb_2S_3 is a kind of stable light absorption materials with suitable band gap, promising for practical applications. Here we demonstrate that the engineering on the composition ratio enables significant improvement in the device performance. We found that the co-evaporation of sulfur or antimony with Sb_2S_3 is able to generate sulfur-or antimony-rich Sb_2S_3. This composition does not generate essential influence on the crystal structure, optical band and film formability, while the carrier concentration and transport dynamics are considerably changed. The device investigations show that sulfur-rich Sb_2S_3 film is favorable for efficient energy conversion, while antimony-rich Sb_2S_3 leads to greatly decreased device performance. With optimizations on the sulfur-rich Sb_2S_3 films, the final power conversion efficiency reaches5.8%, which is the highest efficiency in thermal evaporation derived Sb_2S_3 solar cells.
基金supported by the State Key Program of the National Natural Science Foundation of China(Grant No.91643206)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB14030300)the Chinese Academy of Sciences/State Administration of Foreign Experts Affairs(CAS/SAFEA)International Partnership Program for Creative Research Teams of China
文摘Exposure of airborne particulate matter(PM)with an aerodynamic diameter less than 2.5μm(PM2.5)is epidemiologically associated with lung dysfunction and respiratory symptoms,including pulmonary fibrosis.However,whether epigenetic mechanisms are involved in PM2.5-induced pulmonary fibrosis is currently poorly understood.Herein,using a PM2.5-induced pulmonary fibrosis mouse model,we found that PM2.5 exposure leads to aberrant mRNA5-methylcytosine(m5C)gain and loss in fibrotic lung tissues.Moreover,we showed the m5C-mediated regulatory map of gene functions in pulmonary fibrosis after PM2.5 exposure.Several genes act as m5C gain-upregulated factors,probably critical for the development of PM2.5-induced fibrosis in mouse lungs.These genes,including Lcn2,Mmp9,Chi3l1,Adipoq,Atp5j2,Atp5l,Atpif1,Ndufb6,Fgr,Slc11 a1,and Tyrobp,are highly related to oxidative stress response,inflammatory responses,and immune system processes.Our study illustrates the first epitranscriptomic RNA m5C profile in PM2.5-induced pulmonary fibrosis and will be valuable in identifying biomarkers for PM2.5 exposure-related lung pathogenesis with translational potential.
