Objective: To identify the chemical constituents and biological activities of essential oil and crude methanol extract of Artemisia vulgaris(A.vulgaris) and Gaultheria fragrantissima(G.fragrantissima).Methods: Phytoch...Objective: To identify the chemical constituents and biological activities of essential oil and crude methanol extract of Artemisia vulgaris(A.vulgaris) and Gaultheria fragrantissima(G.fragrantissima).Methods: Phytochemical screening, total phenolic and flavonoid content, antibacterial activities, anti-oxidant assay of the crude extract were carried out to identify the biological activities and phytonutrients present in the extract.Furthermore, the chemical constituents present in the essential oil and crude methanol extract were analyzed using gas chromatography mass spectroscopy and high performance liquid chromatography(HPLC) analysis.Results: Gas chromatography mass spectroscopy analysis of essential oil from the aerial part of A.vulgaris revealed 24 different compounds in it.Sabinene(11.29%), b-thujone(19.19%), chrysanthenone(4.48%), camphor(11.89%), borneol(4.44%) and germacrene D(8.42%) were the major compounds.Similarly, leaves of G.fragrantissima contained methyl salicylate(95%) and asarone(4.64%).Furthermore, methanol extract of leaves of A.vulgaris and G.fragrantissima were found rich in the total flavonoids and phenolic content.HPLC analysis of the methanol extract of leaves A.vulgaris revealed the presence of morin and luteolin, whereas rutin was found as a major flavonoids compound in the leaves of G.fragrantissima.Further, methanol extract of the A.vulgaris and G.fragrantissima showed the highest antioxidant and antibacterial properties compared to the essential oil.Conclusions: The HPLC analysis of the methanol extract of A.vulgaris shows the presence of luteolin and morin, whereas G.fragrantissima reveals the presence of rutin and a glycosylated flavonoids.Results reveal that A.vulgaris oil is the rich source of monoterpene and sesquiterpene compounds.Furthermore, A.vulgaris and G.fragrantissima are the rich source of the phenolic and flavonoids compounds and show good antioxidant and antibacterial activity.展开更多
The effect on the germination and seedling growth of radish(Raphanus sativus)seeds were examined employing a dielectric barrier discharge(DBD)at atmospheric pressure and room temperature for various treatment time.DBD...The effect on the germination and seedling growth of radish(Raphanus sativus)seeds were examined employing a dielectric barrier discharge(DBD)at atmospheric pressure and room temperature for various treatment time.DBD plasma using argon gas of flow rate 21 m^(-1)was employed in this study.Radish seeds were treated with DBD plasma for 1-5 min,respectively.Germination characteristics,seedling growth parameters,the contact angle of the seed coat,water uptake capacity,mass loss,the temperature of the seeds,chlorophyll,and carotenoid contents of the seedlings were measured before and after the DBD plasma treatments.Plasma treatment of radish seeds significantly increased germination-related characters,including germination percentage,fresh and dry weight,vigor index,and total carotenoids contents.However,the cumulative production rate was found to be decreased.Results from the experiment indicate an acceleration in the water uptake of the radish seeds and make the seed surface hydrophilic by plasma treatment.Scanning electron microscopy analysis showed that etching effects on the seed coat occurred after the argon plasma treatments,which affected the wettability of the radish seed.The experimental findings showed that seeds being treated by DBD plasma for 2 and 3 min had a positive effect on the germination and seedling growth of radish.展开更多
Background:Murraya koenigii(L.)Spreng.(Rutaceae)is an evergreen shrub distributed over the sub-tropical to lower temperate climatic zone of Nepal which has been used as spice in culinary practice and traditionally bei...Background:Murraya koenigii(L.)Spreng.(Rutaceae)is an evergreen shrub distributed over the sub-tropical to lower temperate climatic zone of Nepal which has been used as spice in culinary practice and traditionally being used to treat bites of poisonous animals,dysentery,skin diseases,vomiting,diarrhea,and inflammations.Objective:The main objective of this study is to study the enzyme inhibition potential of different extracts of M.koenigii leaves collected from western Nepal.Methods:Total flavonoid content,total phenolic content,in vitro antioxidant,𝛼α-amylase,lipase,elastase,tyrosinase,and cholinesterase inhibition activities analysis of M.koenigii in ethyl acetate,methanol,and water extract were evaluated.In addition,high-resolution mass spectrometry-based profiling of flavonoid molecules and their docking analysis were performed.Results:Results revealed the noteworthy radical scavenging activity of ethyl acetate extract against ABTS and DPPH.Among the analyzed enzymes,M.koenigii extracts revealed the highest inhibitory potential towards acetylcholinesterase(AChE)and butyrylcholinesterase(BChE)and only water extract showed the inhibition of tyrosinase.The HPLC-ESI-QTOF-MS analysis revealed the presence of Rutin,Quercetin,Ferulic acid,Hyperoside,Kaempferol-7-O-glucoside,and Morin in M.koenigii.Further,in silico docking analysis revealed the significant binding energy of these flavonoid molecules;especially Rutin with BChE.Conclusion:The ethyl acetate and methanolic extracts of M.koenigii showed paramount flavonoid and phenolic content along with noteworthy potential towardsthe inhibition of neuro-degradative enzymes such as BChE which can be characterized further for pharmacological applications.Further study on the isolation of metabolites and their pharmacological properties from M.koenigii is recommended.展开更多
基金supported by Academy Partnership Program Track 1-1 funded by KOICA(APP-Track 1-1 2015-0249)
文摘Objective: To identify the chemical constituents and biological activities of essential oil and crude methanol extract of Artemisia vulgaris(A.vulgaris) and Gaultheria fragrantissima(G.fragrantissima).Methods: Phytochemical screening, total phenolic and flavonoid content, antibacterial activities, anti-oxidant assay of the crude extract were carried out to identify the biological activities and phytonutrients present in the extract.Furthermore, the chemical constituents present in the essential oil and crude methanol extract were analyzed using gas chromatography mass spectroscopy and high performance liquid chromatography(HPLC) analysis.Results: Gas chromatography mass spectroscopy analysis of essential oil from the aerial part of A.vulgaris revealed 24 different compounds in it.Sabinene(11.29%), b-thujone(19.19%), chrysanthenone(4.48%), camphor(11.89%), borneol(4.44%) and germacrene D(8.42%) were the major compounds.Similarly, leaves of G.fragrantissima contained methyl salicylate(95%) and asarone(4.64%).Furthermore, methanol extract of leaves of A.vulgaris and G.fragrantissima were found rich in the total flavonoids and phenolic content.HPLC analysis of the methanol extract of leaves A.vulgaris revealed the presence of morin and luteolin, whereas rutin was found as a major flavonoids compound in the leaves of G.fragrantissima.Further, methanol extract of the A.vulgaris and G.fragrantissima showed the highest antioxidant and antibacterial properties compared to the essential oil.Conclusions: The HPLC analysis of the methanol extract of A.vulgaris shows the presence of luteolin and morin, whereas G.fragrantissima reveals the presence of rutin and a glycosylated flavonoids.Results reveal that A.vulgaris oil is the rich source of monoterpene and sesquiterpene compounds.Furthermore, A.vulgaris and G.fragrantissima are the rich source of the phenolic and flavonoids compounds and show good antioxidant and antibacterial activity.
基金Nepal Academy of Science and Technology(NAST),Khumaltar,Lalitpur,Nepal for providing financial and technical support。
文摘The effect on the germination and seedling growth of radish(Raphanus sativus)seeds were examined employing a dielectric barrier discharge(DBD)at atmospheric pressure and room temperature for various treatment time.DBD plasma using argon gas of flow rate 21 m^(-1)was employed in this study.Radish seeds were treated with DBD plasma for 1-5 min,respectively.Germination characteristics,seedling growth parameters,the contact angle of the seed coat,water uptake capacity,mass loss,the temperature of the seeds,chlorophyll,and carotenoid contents of the seedlings were measured before and after the DBD plasma treatments.Plasma treatment of radish seeds significantly increased germination-related characters,including germination percentage,fresh and dry weight,vigor index,and total carotenoids contents.However,the cumulative production rate was found to be decreased.Results from the experiment indicate an acceleration in the water uptake of the radish seeds and make the seed surface hydrophilic by plasma treatment.Scanning electron microscopy analysis showed that etching effects on the seed coat occurred after the argon plasma treatments,which affected the wettability of the radish seed.The experimental findings showed that seeds being treated by DBD plasma for 2 and 3 min had a positive effect on the germination and seedling growth of radish.
基金supported by a UGC collaborative research grant(Award No:CRG-78/79-S&T-01)and also supported by H-Plant Nepal Private Limited.
文摘Background:Murraya koenigii(L.)Spreng.(Rutaceae)is an evergreen shrub distributed over the sub-tropical to lower temperate climatic zone of Nepal which has been used as spice in culinary practice and traditionally being used to treat bites of poisonous animals,dysentery,skin diseases,vomiting,diarrhea,and inflammations.Objective:The main objective of this study is to study the enzyme inhibition potential of different extracts of M.koenigii leaves collected from western Nepal.Methods:Total flavonoid content,total phenolic content,in vitro antioxidant,𝛼α-amylase,lipase,elastase,tyrosinase,and cholinesterase inhibition activities analysis of M.koenigii in ethyl acetate,methanol,and water extract were evaluated.In addition,high-resolution mass spectrometry-based profiling of flavonoid molecules and their docking analysis were performed.Results:Results revealed the noteworthy radical scavenging activity of ethyl acetate extract against ABTS and DPPH.Among the analyzed enzymes,M.koenigii extracts revealed the highest inhibitory potential towards acetylcholinesterase(AChE)and butyrylcholinesterase(BChE)and only water extract showed the inhibition of tyrosinase.The HPLC-ESI-QTOF-MS analysis revealed the presence of Rutin,Quercetin,Ferulic acid,Hyperoside,Kaempferol-7-O-glucoside,and Morin in M.koenigii.Further,in silico docking analysis revealed the significant binding energy of these flavonoid molecules;especially Rutin with BChE.Conclusion:The ethyl acetate and methanolic extracts of M.koenigii showed paramount flavonoid and phenolic content along with noteworthy potential towardsthe inhibition of neuro-degradative enzymes such as BChE which can be characterized further for pharmacological applications.Further study on the isolation of metabolites and their pharmacological properties from M.koenigii is recommended.
