本文通过考察血酮(血液中酮体)浓度、产羔情况及粪便微生物区系组成,评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)缓解围产期母羊能量负平衡的效果。试验期共57 d,从产前30 d到产后20 d,预饲期7 d。选取80只妊娠期(120~130 d)湖羊,随机分为4...本文通过考察血酮(血液中酮体)浓度、产羔情况及粪便微生物区系组成,评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)缓解围产期母羊能量负平衡的效果。试验期共57 d,从产前30 d到产后20 d,预饲期7 d。选取80只妊娠期(120~130 d)湖羊,随机分为4组,对照组饲喂基础饲粮,RPC组每只每天添加3 g RPC,RPG组添加15 g RPG,RPC+RPG组添加3 g RPC和15 g RPG。结果表明,1)RPG组母羊在产羔当天血酮浓度显著低于对照组(P<0.05);2)RPC+RPG组羔羊初生质量显著高于其他组(P<0.05);3)RPC和RPG的添加没有改变门水平和属水平上母羊粪便的优势菌种类,但影响了核心菌群的相对丰度,各试验组厚壁菌门和瘤胃球菌科UCG-010相对丰度均显著低于对照组(P<0.05),RPC+RPG组拟杆菌门相对丰度显著高于其他组(P<0.05),RPG组毛螺菌科AC2044群相对丰度显著高于对照组(P<0.05)。由此得出,围产期母羊饲粮中添加RPC和RPG均有助于提高羔羊初生质量,提高母羊肠道中碳水化合物利用菌拟杆菌门的相对丰度,降低纤维分解菌群厚壁菌门的相对丰度,RPG的添加还使得肠道抗炎有益菌毛螺菌科属相对丰度得到提高,同时有效降低血酮浓度。综合考虑,RPG单独添加效果最佳,RPC的适宜添加量有待进一步研究。展开更多
This experiment was conducted to study the effect of molasses on the fermentation characteristics of mixed silage ensiled rice straw and vegetable by-products with alfalfa.Mixture（202 g kg^-1 dry matter（DM））consis...This experiment was conducted to study the effect of molasses on the fermentation characteristics of mixed silage ensiled rice straw and vegetable by-products with alfalfa.Mixture（202 g kg^-1 dry matter（DM））consisting of rice straw,broccoli residue and alfalfa at the ratio of 5：4：1 was ensiled with three experimental treatments：（1）no additives（control）;（2）molasses at 2.5%（M1）;（3）molasses at 5%（M2）on a fresh matter basis of mixture,respectively.All treatments were packed into laboratory-scale silos,and three silos per treatment were sampled on days 1,3,5,14 and 30.The result showed that the p H value of all mixed silages decreased gradually with the time of ensiling except for the control silage,in which a significant increase（P〈0.05）on day 30 occurred.The lactic acid content increased gradually with the time of ensiling and reached the highest value on day 14,and a marked decrease（P〈0.05）was found in the control silage on day 30.The changes of acetic acid content showed similar pattern with lactic acid content.A trace amount of propionic and butyric acid contents were found in the three mixed silages during the fermentation period.Comparing to the control,M1 and M2 treatments improved the fermentation quality of mixed silages as indicated by higher（P〈0.05）lactic acid contents and lower（P〈0.05）p H and ammonia-N contents.The Flieg points also showed that M1 and M2 silages were well preserved,whereas the control silage had a bad quality.Overall,the findings of this study suggested that adding molasses could improve fermentation quality of mixed silage,and M1 was more suitable for practical application.展开更多
Reconstructed synsedimentary paleogeomorpholgy is an effective method in predicting the distribution of sandbodies and can provide valid evidence in the search for reservoirs. Based on a synsedimentary paleogeomorphol...Reconstructed synsedimentary paleogeomorpholgy is an effective method in predicting the distribution of sandbodies and can provide valid evidence in the search for reservoirs. Based on a synsedimentary paleogeomorpholgy reconstruction of the third member of the Dongying formation (Ed3) in the Qikou sag,the basic paleogeomorphic characteristics of Ed3 are described and the spatial distributions of denudation and subsidence areas are discussed. Key boundary faults controlling the deposition of important sediment bodies are proposed and the interrelations among faulted slope-break belts or flexure belts,intra-depression uplifts and sags,sediment sources and sediments input points have been investigated.展开更多
A new fluorescent reagent bis（2-diazoaminobenzothiazoyl）-benzidine（BDABTB） was synthesized by combining benzothiazole with benzidine and triazene reagent. The product was confirmed by elemental analysis, ^1H NMR a...A new fluorescent reagent bis（2-diazoaminobenzothiazoyl）-benzidine（BDABTB） was synthesized by combining benzothiazole with benzidine and triazene reagent. The product was confirmed by elemental analysis, ^1H NMR and FTIR. The excitation and emission wavelengths of BDABTB were 214 and 338 nm in a basic medium, respectively. The fluorescence was quenched by Hg^2＋ selectively and sensitively. The linear range of Hg^2＋ was 1.0×10^-8-1.0×10^-5 mol/L with a detection limit of 5.0×10^-10 mol/L. It has been used to determine trace Hg^2＋ in water sample with satisfactory results.展开更多
Finish effect of four feeding patterns on weaned lamb was studied in the paper. The results showed that the performance of growing lambs by agricultural byproduct residual forage was superior to other feeding methods;...Finish effect of four feeding patterns on weaned lamb was studied in the paper. The results showed that the performance of growing lambs by agricultural byproduct residual forage was superior to other feeding methods; too high proportion of straw in diet was unfavorable for finish lamb, and corn straw should be combined with complete feed to get better feeding effect.展开更多
文摘本文通过考察血酮(血液中酮体)浓度、产羔情况及粪便微生物区系组成,评估过瘤胃胆碱(RPC)和过瘤胃葡萄糖(RPG)缓解围产期母羊能量负平衡的效果。试验期共57 d,从产前30 d到产后20 d,预饲期7 d。选取80只妊娠期(120~130 d)湖羊,随机分为4组,对照组饲喂基础饲粮,RPC组每只每天添加3 g RPC,RPG组添加15 g RPG,RPC+RPG组添加3 g RPC和15 g RPG。结果表明,1)RPG组母羊在产羔当天血酮浓度显著低于对照组(P<0.05);2)RPC+RPG组羔羊初生质量显著高于其他组(P<0.05);3)RPC和RPG的添加没有改变门水平和属水平上母羊粪便的优势菌种类,但影响了核心菌群的相对丰度,各试验组厚壁菌门和瘤胃球菌科UCG-010相对丰度均显著低于对照组(P<0.05),RPC+RPG组拟杆菌门相对丰度显著高于其他组(P<0.05),RPG组毛螺菌科AC2044群相对丰度显著高于对照组(P<0.05)。由此得出,围产期母羊饲粮中添加RPC和RPG均有助于提高羔羊初生质量,提高母羊肠道中碳水化合物利用菌拟杆菌门的相对丰度,降低纤维分解菌群厚壁菌门的相对丰度,RPG的添加还使得肠道抗炎有益菌毛螺菌科属相对丰度得到提高,同时有效降低血酮浓度。综合考虑,RPG单独添加效果最佳,RPC的适宜添加量有待进一步研究。
基金supported by the project of Jiangsu Independent Innovation, China (CX(15)1003-3)the Key Technologies R&D Program of China during the 13th Five Year Plan period (2016YFC0502005)the special project of grass of Tibetan Autonomous Region for the “13th FiveYear” Plan, China
文摘This experiment was conducted to study the effect of molasses on the fermentation characteristics of mixed silage ensiled rice straw and vegetable by-products with alfalfa.Mixture（202 g kg^-1 dry matter（DM））consisting of rice straw,broccoli residue and alfalfa at the ratio of 5：4：1 was ensiled with three experimental treatments：（1）no additives（control）;（2）molasses at 2.5%（M1）;（3）molasses at 5%（M2）on a fresh matter basis of mixture,respectively.All treatments were packed into laboratory-scale silos,and three silos per treatment were sampled on days 1,3,5,14 and 30.The result showed that the p H value of all mixed silages decreased gradually with the time of ensiling except for the control silage,in which a significant increase（P〈0.05）on day 30 occurred.The lactic acid content increased gradually with the time of ensiling and reached the highest value on day 14,and a marked decrease（P〈0.05）was found in the control silage on day 30.The changes of acetic acid content showed similar pattern with lactic acid content.A trace amount of propionic and butyric acid contents were found in the three mixed silages during the fermentation period.Comparing to the control,M1 and M2 treatments improved the fermentation quality of mixed silages as indicated by higher（P〈0.05）lactic acid contents and lower（P〈0.05）p H and ammonia-N contents.The Flieg points also showed that M1 and M2 silages were well preserved,whereas the control silage had a bad quality.Overall,the findings of this study suggested that adding molasses could improve fermentation quality of mixed silage,and M1 was more suitable for practical application.
基金Projects 40872077 supported by the National Natural Science Foundation of China2008CDA098 by the Key Natural Science Foundation of Hubei Province
文摘Reconstructed synsedimentary paleogeomorpholgy is an effective method in predicting the distribution of sandbodies and can provide valid evidence in the search for reservoirs. Based on a synsedimentary paleogeomorpholgy reconstruction of the third member of the Dongying formation (Ed3) in the Qikou sag,the basic paleogeomorphic characteristics of Ed3 are described and the spatial distributions of denudation and subsidence areas are discussed. Key boundary faults controlling the deposition of important sediment bodies are proposed and the interrelations among faulted slope-break belts or flexure belts,intra-depression uplifts and sags,sediment sources and sediments input points have been investigated.
基金Supported by the National Natural Science Foundation of China(No.20575057)the Natural Science Foundation of Shanxi Province,China(No.2009011015-1)
文摘A new fluorescent reagent bis（2-diazoaminobenzothiazoyl）-benzidine（BDABTB） was synthesized by combining benzothiazole with benzidine and triazene reagent. The product was confirmed by elemental analysis, ^1H NMR and FTIR. The excitation and emission wavelengths of BDABTB were 214 and 338 nm in a basic medium, respectively. The fluorescence was quenched by Hg^2＋ selectively and sensitively. The linear range of Hg^2＋ was 1.0×10^-8-1.0×10^-5 mol/L with a detection limit of 5.0×10^-10 mol/L. It has been used to determine trace Hg^2＋ in water sample with satisfactory results.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201203050-4)Agricultural Science and Technology Innovation Funds of Jiangsu Province[CX(11)2050]
文摘Finish effect of four feeding patterns on weaned lamb was studied in the paper. The results showed that the performance of growing lambs by agricultural byproduct residual forage was superior to other feeding methods; too high proportion of straw in diet was unfavorable for finish lamb, and corn straw should be combined with complete feed to get better feeding effect.
