Objective:To investigate the protective role of Ocimum sanctum（O.sanctum） leaves against Salmonella typhim.urium（S.typhimurium） infection in rats by inducing TNF-α,IFN-γ& IL-2 cytokines.Methods:Wistar albino...Objective:To investigate the protective role of Ocimum sanctum（O.sanctum） leaves against Salmonella typhim.urium（S.typhimurium） infection in rats by inducing TNF-α,IFN-γ& IL-2 cytokines.Methods:Wistar albino rats were fed with aqueous extract of 0.sanctum leaves using 250 mg/kg body weight dose once a day for 20 consecutive days.Control rats were fed with placebo.Rats were infected with LD<sub>50</sub> dose of 5.typhimurium infection and monitored for their survival.Bacterial blood burden in both the groups was compared and numbers of activated peritoneal macrophages were counted.Concentration of TNF-α,IFN-γand IL-2 cytokines in serum during different time intervals was assayed by sandwich ELISA.Results:Rats of control group showed a high mortality rate and had higher bacterial blood burden when compared with O.sanctum extract fed rats.There was a significant increase in the number of S.typhimurium engulfed peritoneal macrophages in the peritoneal fluid of O.sanctum fed animals.The protective control against bacterial infection in O,sanctum fed rats was associated with elevated level of TNF-α,IFN-γand IL-2 cytokines in serum.Conclusions:These findings suggest that orally administered O.sanctum leaves extract effectively enhanced activation in macrophage and lymphocytes,depicted by the elevated serum concentration of TNF-α,IFN-γand IL-2 cytokines,leading to induce a protective resistance against Salmonella typhimurium infection.展开更多
Objective:To investigate the regulatory effect of aqueous extract of leaves of Ocimum sanctum on IL-2 cytokine production in vivo and in vitro,and the effect of leaves extract on general blood picture including T&...Objective:To investigate the regulatory effect of aqueous extract of leaves of Ocimum sanctum on IL-2 cytokine production in vivo and in vitro,and the effect of leaves extract on general blood picture including T& B lymphocytes.Methods:For in vivo studies albino Wistar rats were treated with aqueous crude leaves extract of Ocimum sanctum for 20 consecutive days.Spleen cells were harvested and assayed for IL-2 production by using sandwich enzyme-linked immunosorbent assay(ELISA) and mRNA expression methods.For in vitro study aqueous Ocimum sanctum leaves extract= in different concentrations(25-500μ/mL) was added into culture plates containing ConA stimulated splenocytes.To study the overall effect on blood picture,density gradient purified lymphocytes analysis and conventional methodology for total and differential leukocyte count and hemoglobin level were also done.Results:It indicated that the rats treated with Ocimum sanctum leaves extract had significantly enhanced(P【0.001) ability of spleen cells to secrete IL-2.Investigation in vitro also showed regulation of IL-2 production.Blood study exhibited leucocytosis and augmentation of T& B lymphocytes by 25%approximately.4-5%increase in Hemoglobin value was also noticed.Conclusion:Aqueous Ocimum sanctum leaves extract may have stimulatory effect on T & B lymphocytes particularly on Th 1 subset of lymphocytes as shown by enhancement in IL-2 production.展开更多
基金Dept.Of Science & Technology, New Delhi for providing the financial grant
文摘Objective:To investigate the protective role of Ocimum sanctum（O.sanctum） leaves against Salmonella typhim.urium（S.typhimurium） infection in rats by inducing TNF-α,IFN-γ& IL-2 cytokines.Methods:Wistar albino rats were fed with aqueous extract of 0.sanctum leaves using 250 mg/kg body weight dose once a day for 20 consecutive days.Control rats were fed with placebo.Rats were infected with LD<sub>50</sub> dose of 5.typhimurium infection and monitored for their survival.Bacterial blood burden in both the groups was compared and numbers of activated peritoneal macrophages were counted.Concentration of TNF-α,IFN-γand IL-2 cytokines in serum during different time intervals was assayed by sandwich ELISA.Results:Rats of control group showed a high mortality rate and had higher bacterial blood burden when compared with O.sanctum extract fed rats.There was a significant increase in the number of S.typhimurium engulfed peritoneal macrophages in the peritoneal fluid of O.sanctum fed animals.The protective control against bacterial infection in O,sanctum fed rats was associated with elevated level of TNF-α,IFN-γand IL-2 cytokines in serum.Conclusions:These findings suggest that orally administered O.sanctum leaves extract effectively enhanced activation in macrophage and lymphocytes,depicted by the elevated serum concentration of TNF-α,IFN-γand IL-2 cytokines,leading to induce a protective resistance against Salmonella typhimurium infection.
基金financially supported by Woman Scientist Scheme-A to the first author from the Department of Science and Technology,New Delhi
文摘Objective:To investigate the regulatory effect of aqueous extract of leaves of Ocimum sanctum on IL-2 cytokine production in vivo and in vitro,and the effect of leaves extract on general blood picture including T& B lymphocytes.Methods:For in vivo studies albino Wistar rats were treated with aqueous crude leaves extract of Ocimum sanctum for 20 consecutive days.Spleen cells were harvested and assayed for IL-2 production by using sandwich enzyme-linked immunosorbent assay(ELISA) and mRNA expression methods.For in vitro study aqueous Ocimum sanctum leaves extract= in different concentrations(25-500μ/mL) was added into culture plates containing ConA stimulated splenocytes.To study the overall effect on blood picture,density gradient purified lymphocytes analysis and conventional methodology for total and differential leukocyte count and hemoglobin level were also done.Results:It indicated that the rats treated with Ocimum sanctum leaves extract had significantly enhanced(P【0.001) ability of spleen cells to secrete IL-2.Investigation in vitro also showed regulation of IL-2 production.Blood study exhibited leucocytosis and augmentation of T& B lymphocytes by 25%approximately.4-5%increase in Hemoglobin value was also noticed.Conclusion:Aqueous Ocimum sanctum leaves extract may have stimulatory effect on T & B lymphocytes particularly on Th 1 subset of lymphocytes as shown by enhancement in IL-2 production.
