Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein(bovine serum albumin) and a typical fluorophore(rhodamine B) to demonstrate a direct labeling met...Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein(bovine serum albumin) and a typical fluorophore(rhodamine B) to demonstrate a direct labeling method just by physical adsorption. In combination with size exclusion chromatography and the Scartchard equation, we have developed a facile analysis method for calculating the binding constant and binding sites.The molecular docking method has been used to study the binding site in amino acid level.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.21171086 and 81160213)Inner Mongolia Autonomous Region science and Technology Department(No.211-202077)+2 种基金Inner Mongolia Grassland Talent(No.108-108038)Natural Science Foundation of Inner Mongolia Autonomous Region of China(No.2013MS1121)Inner Mongolia Agricultural University(Nos.109-108040,211-109003 and 211-206038)
文摘Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein(bovine serum albumin) and a typical fluorophore(rhodamine B) to demonstrate a direct labeling method just by physical adsorption. In combination with size exclusion chromatography and the Scartchard equation, we have developed a facile analysis method for calculating the binding constant and binding sites.The molecular docking method has been used to study the binding site in amino acid level.
