Plant peroxidase （POD） belongs to multigene family, which not is only one of the important enzymes responsible for the removal of active oxygen radicals, but also participates in a variety of physiological and bioch...Plant peroxidase （POD） belongs to multigene family, which not is only one of the important enzymes responsible for the removal of active oxygen radicals, but also participates in a variety of physiological and biochemical processes and plays a crucial role in the maintenance of plant growth and development. In this study, the structures and functions of proteins encoded by 73 gene of POD family in Arabidopsis were analyzed with bioinformatics method, including the number of amino acids, isoelectric point, transmemberane domains, signal peptides, secondary structures and phosphorylation sites, and the phylogenic trees with and without signal peptides were constructed by using Mega4.0 software, to investigate the structural characteristics. In addition, the structures of AtPER members were analyzed, to reveal the relationship between the structures and functions, thereby providing theoretical basis for the research of plant oxidative stress resistance.展开更多
[目的]寻找一种有效的磁铁矿尾矿宏基因组DNA的提取方法。[方法]分别采用直接提取法和SDS高-盐法提取邯邢矿区磁铁矿尾矿宏基因组DNA,比较2种方法的提取效果。[结果]直接提取法的提取效果较差,基本上没有提出DNA,SDS高-盐法的提取效果较...[目的]寻找一种有效的磁铁矿尾矿宏基因组DNA的提取方法。[方法]分别采用直接提取法和SDS高-盐法提取邯邢矿区磁铁矿尾矿宏基因组DNA,比较2种方法的提取效果。[结果]直接提取法的提取效果较差,基本上没有提出DNA,SDS高-盐法的提取效果较好,DNA得率较高;23 S rDNA特异序列扩增结果显示,所提DNA能够满足PCR扩增的需要。[结论]所取尾矿样品中含有大量的金属离子,这些金属离子不但影响溶菌酶的活性,而且影响后续PCR扩增效率,所以提取DNA前需对样品进行高浓度TE处理。展开更多
基金Supported by National Natural Science Foundation of China(31070361)the Fundamental Research Funds for the Central Universities(0910KYZY43,1112KYQN31)+1 种基金"985 Project"from Minzu University of China(MUC98504-14)Scientific Research Project from State Ethnic Affairs Commission(10ZY01)~~
文摘Plant peroxidase （POD） belongs to multigene family, which not is only one of the important enzymes responsible for the removal of active oxygen radicals, but also participates in a variety of physiological and biochemical processes and plays a crucial role in the maintenance of plant growth and development. In this study, the structures and functions of proteins encoded by 73 gene of POD family in Arabidopsis were analyzed with bioinformatics method, including the number of amino acids, isoelectric point, transmemberane domains, signal peptides, secondary structures and phosphorylation sites, and the phylogenic trees with and without signal peptides were constructed by using Mega4.0 software, to investigate the structural characteristics. In addition, the structures of AtPER members were analyzed, to reveal the relationship between the structures and functions, thereby providing theoretical basis for the research of plant oxidative stress resistance.
文摘[目的]寻找一种有效的磁铁矿尾矿宏基因组DNA的提取方法。[方法]分别采用直接提取法和SDS高-盐法提取邯邢矿区磁铁矿尾矿宏基因组DNA,比较2种方法的提取效果。[结果]直接提取法的提取效果较差,基本上没有提出DNA,SDS高-盐法的提取效果较好,DNA得率较高;23 S rDNA特异序列扩增结果显示,所提DNA能够满足PCR扩增的需要。[结论]所取尾矿样品中含有大量的金属离子,这些金属离子不但影响溶菌酶的活性,而且影响后续PCR扩增效率,所以提取DNA前需对样品进行高浓度TE处理。
