抗体工程是随着重组 DNA 技术的不断进步和分子免疫学研究的日臻深入而逐渐发展起来的一项生物高新技术。它是指在基因水平上,模拟抗体产生过程,改造抗体结构和强化抗体功能的一系列新兴生物工程技术手段。它的出现,为方便大量、快速地...抗体工程是随着重组 DNA 技术的不断进步和分子免疫学研究的日臻深入而逐渐发展起来的一项生物高新技术。它是指在基因水平上,模拟抗体产生过程,改造抗体结构和强化抗体功能的一系列新兴生物工程技术手段。它的出现,为方便大量、快速地制备高效的抗体,使之广泛应用于医学领域成为一种有效的治疗手段提供了可能。近年来研究人员通过不懈的努力。展开更多
DeveloPment Promoting Factor-1 (DPF-1) was derived from the rabbit oviductmucosa epithelial cells. fertilized eggs were treated with DPF-1 and it was adminis-tered to female mice in order to investigate its effect on...DeveloPment Promoting Factor-1 (DPF-1) was derived from the rabbit oviductmucosa epithelial cells. fertilized eggs were treated with DPF-1 and it was adminis-tered to female mice in order to investigate its effect on embryonic development and implantation. The results of experiment I indicated that of the 82 fertilized eggs cul-tured in the absence of DPF-1, only 9 were normally divided to the morula stage onday4. By contrast, in the medium containing DPF-1 109(90% ) of 121 embryos weredeveloped to the blastocyst stage. In eoperiment Ⅱ, the developed blastocysts weretransferred into the uterus of 3-day pseudopregnant DPF-1-treated or control recipi-ents. Three different combinations of intrauterine transfer were performed: the DPF-1 treated embryos transferred to the control reclpients(DPF- 1→C),the control embryosto the DPF- 1 treated recipients(C→DPF- 1), and the control embryos to the control re-cipients (C→C). The implantation rate was assessed on day 18 after transfer. Therewere significant differences in pregnancy rate per mouse between DPF-1→C and C→DPF- 1 or control groups(C→C). The embryo implantation rate was low in C→DPF-1 (26. 0%, 75/288) and C→ C (25 3%, 73/288) as compared with DPF-1→C(61. 8%, 178/288). These results suggest that the major site of the DPF-1 actionduring early pregnancy is the embryo itself. DPF-1 enables embryos to develop, buthas no obvious direct effect on uterine receptivity.展开更多
文摘抗体工程是随着重组 DNA 技术的不断进步和分子免疫学研究的日臻深入而逐渐发展起来的一项生物高新技术。它是指在基因水平上,模拟抗体产生过程,改造抗体结构和强化抗体功能的一系列新兴生物工程技术手段。它的出现,为方便大量、快速地制备高效的抗体,使之广泛应用于医学领域成为一种有效的治疗手段提供了可能。近年来研究人员通过不懈的努力。
文摘DeveloPment Promoting Factor-1 (DPF-1) was derived from the rabbit oviductmucosa epithelial cells. fertilized eggs were treated with DPF-1 and it was adminis-tered to female mice in order to investigate its effect on embryonic development and implantation. The results of experiment I indicated that of the 82 fertilized eggs cul-tured in the absence of DPF-1, only 9 were normally divided to the morula stage onday4. By contrast, in the medium containing DPF-1 109(90% ) of 121 embryos weredeveloped to the blastocyst stage. In eoperiment Ⅱ, the developed blastocysts weretransferred into the uterus of 3-day pseudopregnant DPF-1-treated or control recipi-ents. Three different combinations of intrauterine transfer were performed: the DPF-1 treated embryos transferred to the control reclpients(DPF- 1→C),the control embryosto the DPF- 1 treated recipients(C→DPF- 1), and the control embryos to the control re-cipients (C→C). The implantation rate was assessed on day 18 after transfer. Therewere significant differences in pregnancy rate per mouse between DPF-1→C and C→DPF- 1 or control groups(C→C). The embryo implantation rate was low in C→DPF-1 (26. 0%, 75/288) and C→ C (25 3%, 73/288) as compared with DPF-1→C(61. 8%, 178/288). These results suggest that the major site of the DPF-1 actionduring early pregnancy is the embryo itself. DPF-1 enables embryos to develop, buthas no obvious direct effect on uterine receptivity.
