The existence of cultured male epidermal allografts in 14 bioptic samples of 9 female burned cases was identified by detecting Y chromosome DNA fragments with polymerase chain reaction (PCR). Y chromosome DNA fragment...The existence of cultured male epidermal allografts in 14 bioptic samples of 9 female burned cases was identified by detecting Y chromosome DNA fragments with polymerase chain reaction (PCR). Y chromosome DNA fragments were found in all the samples and the longest case of allograft survival was detected in a 92nd-day sample. This suggests that the survival time of cultured human epidermal allografts on the wounds is longer than those of non-cultured ones.展开更多
A new artificial composite allograft for burn wound coverage was presented. It was first madefrom a porous collagen membrane from human dermis and then cultured human epidermal cells and fibroblasts were seeded on the...A new artificial composite allograft for burn wound coverage was presented. It was first madefrom a porous collagen membrane from human dermis and then cultured human epidermal cells and fibroblasts were seeded on the outer and inner surfaces of the membrane respectively. The functions and chracteristics of the artificial allograft were studied in experimental and clinical trials, and clinical application was carried out by placing the allograft directly onto the wound with the fibroblast (inner ) side against the woundsurface. After its take, the transplanted artificial allograft would adhere to the recipient wound without anysign of rejection in the whole period of our observation.展开更多
文摘The existence of cultured male epidermal allografts in 14 bioptic samples of 9 female burned cases was identified by detecting Y chromosome DNA fragments with polymerase chain reaction (PCR). Y chromosome DNA fragments were found in all the samples and the longest case of allograft survival was detected in a 92nd-day sample. This suggests that the survival time of cultured human epidermal allografts on the wounds is longer than those of non-cultured ones.
文摘A new artificial composite allograft for burn wound coverage was presented. It was first madefrom a porous collagen membrane from human dermis and then cultured human epidermal cells and fibroblasts were seeded on the outer and inner surfaces of the membrane respectively. The functions and chracteristics of the artificial allograft were studied in experimental and clinical trials, and clinical application was carried out by placing the allograft directly onto the wound with the fibroblast (inner ) side against the woundsurface. After its take, the transplanted artificial allograft would adhere to the recipient wound without anysign of rejection in the whole period of our observation.
