Objective： This paper attempts to discuss the effects of surviving antisense RNA on doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1. Methods： A surviving antisense eukaryotic vector pcDNA3-SV Vas...Objective： This paper attempts to discuss the effects of surviving antisense RNA on doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1. Methods： A surviving antisense eukaryotic vector pcDNA3-SV Vas prepared in previous study was delivered into PANC-1 by electroperforation. Cell survival fraction and MTT assay were used to investigate the sensibility of transfected cells to doxorubicin. Apoptosis was detected by DNA gel electrophoresis. Results： We obtained two positive cell clone PANC-1/SVVas and PANC-1/neo cells, the growth of PANC-1/SV Vas cells was significantly reduced （P〈0.05）. By MTT assay, the IC50 to doxorubicin of PANC-1/SV Vas, PANC-1/neo and PANC-1 cells were （0.285±0.012） μmol/L, （1.528±0.317） μmol/L and （1.540±0.253） μmol/L respectively, the difference was significant by statistic analysis （P〈0.01）. Agarose gel electrophoresis of genomic DNA from PANC/SV Vas showed typical DNA ladder, but DNA from PANC-1/neo and PANC-1 did not. Conclusion： Survivin antisense RNA could enhance doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-I. This may lay an experimental foundation for further research of gene therapy in pancreatic cancer.展开更多
Objective： To study the relationship between the Survivin expression and the histological grade, status of ER, expression of PS2 and the prognosis of patients with primary invasive breast cancer. Method： By using LS...Objective： To study the relationship between the Survivin expression and the histological grade, status of ER, expression of PS2 and the prognosis of patients with primary invasive breast cancer. Method： By using LSAB and SP immunohistochemical method, the expression of Survivin, PS2 and ER in 95 cases of invasive breast cancer were detected. Results： the positive rate of Survivin was 70.5% （67/95） and the expression of Survivin was positively related to the histological grade and status of PS2 and ER. The survival time after operation of patients without expression of Survivin was longer than those with positive Survivin. Conclusion： These data suggest that Survivin expression may be considered as a new unfavorable prognostic factor of breast cancer.展开更多
基金This work was supported by a grant from the Natural Sciences Foundation of the Inner Mongolia Autonomous Region (No. 200308020605).
文摘Objective： This paper attempts to discuss the effects of surviving antisense RNA on doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-1. Methods： A surviving antisense eukaryotic vector pcDNA3-SV Vas prepared in previous study was delivered into PANC-1 by electroperforation. Cell survival fraction and MTT assay were used to investigate the sensibility of transfected cells to doxorubicin. Apoptosis was detected by DNA gel electrophoresis. Results： We obtained two positive cell clone PANC-1/SVVas and PANC-1/neo cells, the growth of PANC-1/SV Vas cells was significantly reduced （P〈0.05）. By MTT assay, the IC50 to doxorubicin of PANC-1/SV Vas, PANC-1/neo and PANC-1 cells were （0.285±0.012） μmol/L, （1.528±0.317） μmol/L and （1.540±0.253） μmol/L respectively, the difference was significant by statistic analysis （P〈0.01）. Agarose gel electrophoresis of genomic DNA from PANC/SV Vas showed typical DNA ladder, but DNA from PANC-1/neo and PANC-1 did not. Conclusion： Survivin antisense RNA could enhance doxorubicin-induced apoptosis in pancreatic cancer cell line PANC-I. This may lay an experimental foundation for further research of gene therapy in pancreatic cancer.
基金This work was supported by a grant fromthe Natural Sciences Foundation of the Inner MongoliaAutonomous Region (No. 200308020605).
文摘Objective： To study the relationship between the Survivin expression and the histological grade, status of ER, expression of PS2 and the prognosis of patients with primary invasive breast cancer. Method： By using LSAB and SP immunohistochemical method, the expression of Survivin, PS2 and ER in 95 cases of invasive breast cancer were detected. Results： the positive rate of Survivin was 70.5% （67/95） and the expression of Survivin was positively related to the histological grade and status of PS2 and ER. The survival time after operation of patients without expression of Survivin was longer than those with positive Survivin. Conclusion： These data suggest that Survivin expression may be considered as a new unfavorable prognostic factor of breast cancer.
