在湖北利川海拔1100 m上下的2个不同地点和土类上,通过正规的田间小区试验,验证了有机水溶肥料这种新型叶面肥料在高海拔地区大豆上的应用效果。试验结果表明,在大豆生育中期用有机水溶肥料800倍液喷雾三次(每次间隔10~15天),可使大豆...在湖北利川海拔1100 m上下的2个不同地点和土类上,通过正规的田间小区试验,验证了有机水溶肥料这种新型叶面肥料在高海拔地区大豆上的应用效果。试验结果表明,在大豆生育中期用有机水溶肥料800倍液喷雾三次(每次间隔10~15天),可使大豆显著增产,比常规施肥亩增产大豆20 kg左右,净增收100元以上,增幅超过15%。该肥主要促进了大豆果荚数量、株粒数及百粒重提高,从而实现增产,可在相似区域的大豆种植上大面积推广应用。The application effect of organic water-soluble fertilizer, a new type of foliar fertilizer, on soybeans in high-altitude areas was verified through regular field trials at two different locations and soil types at an altitude of around 1100 meters in Lichuan, Hubei. The test results showed that in the middle growth period of soybeans, three times of spray with 800 times of organic water-soluble fertilizer (every 10~15 days) could significantly increase the yield of soybeans. Compared with conventional fertilization, the yield of soybeans per mu increased by about 20 kg, and the net income increased by more than 100 yuan, an increase of more than 15%. This fertilizer mainly promotes the increase in the number of soybean pods, the number of seeds per plant, and the weight of one hundred seeds, thereby achieving yield increase. It can be widely promoted and applied in soybean planting in similar areas.展开更多
目的探讨静脉移植骨髓间充质干细胞(BMSCs)治疗脑梗死的疗效及其机制。方法全骨髓贴壁法分离、培养BMSCs;流式细胞仪鉴定BMSCs;线栓法制作MCAO大鼠模型;采用粘附实验和改良神经功能损伤评分(mNSS)评估大鼠的行为功能恢复;直接免疫荧光...目的探讨静脉移植骨髓间充质干细胞(BMSCs)治疗脑梗死的疗效及其机制。方法全骨髓贴壁法分离、培养BMSCs;流式细胞仪鉴定BMSCs;线栓法制作MCAO大鼠模型;采用粘附实验和改良神经功能损伤评分(mNSS)评估大鼠的行为功能恢复;直接免疫荧光法检测Brdu标记的BMSCs;免疫组织化学染色检测大鼠脑组织NSE、Nogo-A、SYN、Ki-67、GFAP、VEGF表达;Bielshowsky's-Luxol fast blue双染检测神经纤维的变化。结果 BMSCs中表达CD106,低表达CD34、CD45、CD11b,高表达CD90、CD29。BMSCs移植后MCAO大鼠模型的神经功能明显好转(P<0.05),梗死侧的神经纤维明显增多,脑组织SYN、Ki-67、GFAP、VEGF的表达增多(P<0.05),Nogo-A表达降低(P<0.05),NSE无显著变化(P>0.05),脑梗死体积和胶质瘢痕厚度与PBS组比较无统计学差异(P>0.05)。结论静脉移植BMSCs能改善脑梗死的神经功能,其作用机制可能为促进血管再生和突触重建,增强神经纤维修复和内源性细胞增殖。展开更多
文摘在湖北利川海拔1100 m上下的2个不同地点和土类上,通过正规的田间小区试验,验证了有机水溶肥料这种新型叶面肥料在高海拔地区大豆上的应用效果。试验结果表明,在大豆生育中期用有机水溶肥料800倍液喷雾三次(每次间隔10~15天),可使大豆显著增产,比常规施肥亩增产大豆20 kg左右,净增收100元以上,增幅超过15%。该肥主要促进了大豆果荚数量、株粒数及百粒重提高,从而实现增产,可在相似区域的大豆种植上大面积推广应用。The application effect of organic water-soluble fertilizer, a new type of foliar fertilizer, on soybeans in high-altitude areas was verified through regular field trials at two different locations and soil types at an altitude of around 1100 meters in Lichuan, Hubei. The test results showed that in the middle growth period of soybeans, three times of spray with 800 times of organic water-soluble fertilizer (every 10~15 days) could significantly increase the yield of soybeans. Compared with conventional fertilization, the yield of soybeans per mu increased by about 20 kg, and the net income increased by more than 100 yuan, an increase of more than 15%. This fertilizer mainly promotes the increase in the number of soybean pods, the number of seeds per plant, and the weight of one hundred seeds, thereby achieving yield increase. It can be widely promoted and applied in soybean planting in similar areas.
文摘目的探讨静脉移植骨髓间充质干细胞(BMSCs)治疗脑梗死的疗效及其机制。方法全骨髓贴壁法分离、培养BMSCs;流式细胞仪鉴定BMSCs;线栓法制作MCAO大鼠模型;采用粘附实验和改良神经功能损伤评分(mNSS)评估大鼠的行为功能恢复;直接免疫荧光法检测Brdu标记的BMSCs;免疫组织化学染色检测大鼠脑组织NSE、Nogo-A、SYN、Ki-67、GFAP、VEGF表达;Bielshowsky's-Luxol fast blue双染检测神经纤维的变化。结果 BMSCs中表达CD106,低表达CD34、CD45、CD11b,高表达CD90、CD29。BMSCs移植后MCAO大鼠模型的神经功能明显好转(P<0.05),梗死侧的神经纤维明显增多,脑组织SYN、Ki-67、GFAP、VEGF的表达增多(P<0.05),Nogo-A表达降低(P<0.05),NSE无显著变化(P>0.05),脑梗死体积和胶质瘢痕厚度与PBS组比较无统计学差异(P>0.05)。结论静脉移植BMSCs能改善脑梗死的神经功能,其作用机制可能为促进血管再生和突触重建,增强神经纤维修复和内源性细胞增殖。
