[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequence...[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood（ML）method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.展开更多
现就2005年7月至2010年7月在我院住院的16例主动脉夹层的诊疗过程进行回顾性分析,报道如下.
1 临床资料
16例患者中,男性12例,女性4例,年龄36～79岁,平均61.5岁.既往有高血压12例、冠心病7例、马凡综合征l例;胸痛8例、腹痛3例、偏...现就2005年7月至2010年7月在我院住院的16例主动脉夹层的诊疗过程进行回顾性分析,报道如下.
1 临床资料
16例患者中,男性12例,女性4例,年龄36～79岁,平均61.5岁.既往有高血压12例、冠心病7例、马凡综合征l例;胸痛8例、腹痛3例、偏瘫2例、失语吞咽困难l例、双下肢活动受限伴麻木l例、便血l例、腰痛1例.16例均经心脏超声、胸腹部MRI检查符合主动脉夹层改变,结合症状、体征,确诊为主动脉夹层.按DeBakey分型：Ⅰ型6例、Ⅱ型7例、Ⅲ型3例.入院后,对收缩压≥140 mm Hg（1 mm Hg=0.133 kPa）、舒张压≥90 mm Hg者,静脉滴注硝普钠;对血容量不足、血压偏低者予补充血容量,纠正心衰.监测血压、心率及心电图,根据病情变化调节药物剂量,对治疗过程中发生的各种情况予以对症治疗.尽早服用β受体阻滞剂,可联用血管紧张素转换酶抑制剂（ACEI）或钙通道阻滞剂（CCB）,有休克及心功能Ⅲ级以上者暂不用,心功能改善后再服用上述药物.本组2例因主动脉夹层破裂死亡,5例经内科初始治疗病情稳定后转外院行外科手术治疗,9例经内科保守治疗病情好转出院,出院后继续口服药物治疗.展开更多
基金Supported by New Century Program for Excellent Talents of Ministry of Education of China(NCET-07-0251)Talents Foundation of Anhui Province(08040106803)~~
文摘[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood（ML）method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.
文摘现就2005年7月至2010年7月在我院住院的16例主动脉夹层的诊疗过程进行回顾性分析,报道如下.
1 临床资料
16例患者中,男性12例,女性4例,年龄36～79岁,平均61.5岁.既往有高血压12例、冠心病7例、马凡综合征l例;胸痛8例、腹痛3例、偏瘫2例、失语吞咽困难l例、双下肢活动受限伴麻木l例、便血l例、腰痛1例.16例均经心脏超声、胸腹部MRI检查符合主动脉夹层改变,结合症状、体征,确诊为主动脉夹层.按DeBakey分型：Ⅰ型6例、Ⅱ型7例、Ⅲ型3例.入院后,对收缩压≥140 mm Hg（1 mm Hg=0.133 kPa）、舒张压≥90 mm Hg者,静脉滴注硝普钠;对血容量不足、血压偏低者予补充血容量,纠正心衰.监测血压、心率及心电图,根据病情变化调节药物剂量,对治疗过程中发生的各种情况予以对症治疗.尽早服用β受体阻滞剂,可联用血管紧张素转换酶抑制剂（ACEI）或钙通道阻滞剂（CCB）,有休克及心功能Ⅲ级以上者暂不用,心功能改善后再服用上述药物.本组2例因主动脉夹层破裂死亡,5例经内科初始治疗病情稳定后转外院行外科手术治疗,9例经内科保守治疗病情好转出院,出院后继续口服药物治疗.
