目的:探讨简易乳胶环改良造口术治疗前庭大腺囊(脓)肿的疗效。方法:对145例前庭大腺囊(脓)肿患者随机分为两组,75例行简易乳胶环改良造口术的患者为观察组,70例行传统造口术的患者为对照组,比较两种手术方式的手术时间、术中出血量、1%...目的:探讨简易乳胶环改良造口术治疗前庭大腺囊(脓)肿的疗效。方法:对145例前庭大腺囊(脓)肿患者随机分为两组,75例行简易乳胶环改良造口术的患者为观察组,70例行传统造口术的患者为对照组,比较两种手术方式的手术时间、术中出血量、1%利多卡因用量及复发率。结果:观察组与对照组相比,手术时间短(8.3±1.3分钟vs 13.2±1.7分钟)、术中出血量少(11.9±2.0ml vs 23.7±4.6ml)、1%利多卡因用量少(8.6±1.0ml vs 12.5±1.6ml),差异均有统计学意义(P<0.01)。复发率两组相似(1.3%与2.9%)(P>0.05)。结论:简易乳胶环行改良造口术治疗前庭大腺囊(脓)肿的效果优于传统造口术。展开更多
By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of re...By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...展开更多
文摘目的:探讨简易乳胶环改良造口术治疗前庭大腺囊(脓)肿的疗效。方法:对145例前庭大腺囊(脓)肿患者随机分为两组,75例行简易乳胶环改良造口术的患者为观察组,70例行传统造口术的患者为对照组,比较两种手术方式的手术时间、术中出血量、1%利多卡因用量及复发率。结果:观察组与对照组相比,手术时间短(8.3±1.3分钟vs 13.2±1.7分钟)、术中出血量少(11.9±2.0ml vs 23.7±4.6ml)、1%利多卡因用量少(8.6±1.0ml vs 12.5±1.6ml),差异均有统计学意义(P<0.01)。复发率两组相似(1.3%与2.9%)(P>0.05)。结论:简易乳胶环行改良造口术治疗前庭大腺囊(脓)肿的效果优于传统造口术。
基金supported by grants from National Natural Sciences Foundation of China(No.30672227,30600668)"973"Program of China(No.2009CB521800)Joint Research Fund for Overseas Chinese,Hong Kong and Macao Young Scholars(No.30628029)
文摘By using a yeast two-hybrid system,a yeast two-hybrid bait vector was constructed and identified for screening of the HPV18 E6-interacting proteins,and its effects on the growth of yeast cells and the activation of reporter genes were investigated.Total mRNA extracted from Hela cells was reversely transcribed into cDNA.Fragment of HPV18 E6 cDNA was amplified using RT-PCR and directly ligated to the pGBKT7 vector.The recombinant plasmid was confirmed by restriction endonuclease analysis and DNA sequencing.Th...