[Objective] The aim was to carry out isozyme analysis of jin silver carp (Hypophthalmichthys molitrix Var Jing). [Method] The isozyme of AAT, EST, α-GPD, GPI, IDH, LDH, MDH, ME, PGM and PROT of muscles and liver in t...[Objective] The aim was to carry out isozyme analysis of jin silver carp (Hypophthalmichthys molitrix Var Jing). [Method] The isozyme of AAT, EST, α-GPD, GPI, IDH, LDH, MDH, ME, PGM and PROT of muscles and liver in two populations of the silver carp (Hypophthalmichthys molitrix): Jin silver carp (a breed through selective breeding) and artificially propagated population bought from Jingzhou city, Hubei Province were examined by horizontal starch gel electrophoresis. [Result] Eighteen loci were observed in two populations. Two loci of GPI* and PGM* in Jing silver carp population and the locus of GPI* in Jingzhou population were polymorphic. The proportions of polymorphic loci (maximum allele frequency≤0.99) of Jing silver carp and Jingzhou populations were 11.11% and 5.56% respectively, expected heterozygosity were 0.015 0 and 0.001 1 respectively. The Nei’s genetic distances were 0.000 59 between two populations. The result of chi-square test of the GPI* gene in two populations showed that their genetic structure has very significant difference. [Conclusion] This study provided a theoretical basis for large-scale extension of Jing silver carp.展开更多
目的 建立病情-麻醉-手术三位一体预测术中心血管并发症风险的新方法.方法 分别以美国麻醉学协会的患者体质分级标准(American Society of Anesthesiologists Physical Status Classification,ASA-PS)、Carrillo's方法作为病情、手...目的 建立病情-麻醉-手术三位一体预测术中心血管并发症风险的新方法.方法 分别以美国麻醉学协会的患者体质分级标准(American Society of Anesthesiologists Physical Status Classification,ASA-PS)、Carrillo's方法作为病情、手术、麻醉风险分级标准,制作风险评估量表.在2016年1月-2016年12月期间对3 543例各科手术患者进行术前风险评估和分级,记录术中心血管并发症.将病情-麻醉-手术的风险等级与术中心血管并发症进行二元逻辑分析,获得回归系数.利用Logistic回归方程,建立病情-麻醉-手术三位一体风险评估数学模型,用三位一体模型对术中心血管并发症进行预测并与直接用ASA-PS建模的模型比较.结果 3 543例患者术中共发生心血管并发症311例(8.78%).三位一体方法中3种元素对术中并发症的贡献大小依次为病情、麻醉和手术元素,回归系数分别为0.886、0.508、0.268;ASA-pS的回归系数为1.089.三位一体方法术中并发症预测公式为logit (P) =-6.298+0.886×ASA-PS等级+0.508×麻醉等级+0.268×手术等级;ASA-PS方法公式为logit(P)=-4.758+ 1.089×ASA-PS.三位一体方法的受试者工作特征曲线(receiver operating characteristic curve,ROC)和ROC曲线下面积(area under the ROC curve,AUROC)为0.809,ASA-PS的AUROC为0.732.结论 与ASA-PS比较,病情-麻醉-手术三位一体风险评估新方法预测术中心血管并发症的效力和拟合度较好.展开更多
文摘[Objective] The aim was to carry out isozyme analysis of jin silver carp (Hypophthalmichthys molitrix Var Jing). [Method] The isozyme of AAT, EST, α-GPD, GPI, IDH, LDH, MDH, ME, PGM and PROT of muscles and liver in two populations of the silver carp (Hypophthalmichthys molitrix): Jin silver carp (a breed through selective breeding) and artificially propagated population bought from Jingzhou city, Hubei Province were examined by horizontal starch gel electrophoresis. [Result] Eighteen loci were observed in two populations. Two loci of GPI* and PGM* in Jing silver carp population and the locus of GPI* in Jingzhou population were polymorphic. The proportions of polymorphic loci (maximum allele frequency≤0.99) of Jing silver carp and Jingzhou populations were 11.11% and 5.56% respectively, expected heterozygosity were 0.015 0 and 0.001 1 respectively. The Nei’s genetic distances were 0.000 59 between two populations. The result of chi-square test of the GPI* gene in two populations showed that their genetic structure has very significant difference. [Conclusion] This study provided a theoretical basis for large-scale extension of Jing silver carp.
文摘目的 建立病情-麻醉-手术三位一体预测术中心血管并发症风险的新方法.方法 分别以美国麻醉学协会的患者体质分级标准(American Society of Anesthesiologists Physical Status Classification,ASA-PS)、Carrillo's方法作为病情、手术、麻醉风险分级标准,制作风险评估量表.在2016年1月-2016年12月期间对3 543例各科手术患者进行术前风险评估和分级,记录术中心血管并发症.将病情-麻醉-手术的风险等级与术中心血管并发症进行二元逻辑分析,获得回归系数.利用Logistic回归方程,建立病情-麻醉-手术三位一体风险评估数学模型,用三位一体模型对术中心血管并发症进行预测并与直接用ASA-PS建模的模型比较.结果 3 543例患者术中共发生心血管并发症311例(8.78%).三位一体方法中3种元素对术中并发症的贡献大小依次为病情、麻醉和手术元素,回归系数分别为0.886、0.508、0.268;ASA-pS的回归系数为1.089.三位一体方法术中并发症预测公式为logit (P) =-6.298+0.886×ASA-PS等级+0.508×麻醉等级+0.268×手术等级;ASA-PS方法公式为logit(P)=-4.758+ 1.089×ASA-PS.三位一体方法的受试者工作特征曲线(receiver operating characteristic curve,ROC)和ROC曲线下面积(area under the ROC curve,AUROC)为0.809,ASA-PS的AUROC为0.732.结论 与ASA-PS比较,病情-麻醉-手术三位一体风险评估新方法预测术中心血管并发症的效力和拟合度较好.
