本研究旨在深度剖析长链非编码RNA P53和P21在大鼠动脉粥样硬化模型中的作用及应用潜能。择取60只大鼠,随机平均划分为正常组与粥样硬化组,借由构建大鼠动脉粥样硬化模型,综合运用多种实验技术(如免疫组织化学染色、实时荧光定量PCR、...本研究旨在深度剖析长链非编码RNA P53和P21在大鼠动脉粥样硬化模型中的作用及应用潜能。择取60只大鼠,随机平均划分为正常组与粥样硬化组,借由构建大鼠动脉粥样硬化模型,综合运用多种实验技术(如免疫组织化学染色、实时荧光定量PCR、蛋白质印迹法、酶联免疫分析等)及仪器(如彩色多普勒超声诊断仪),对两组中P53和P21的表达状况、调控机制及其与疾病演进的关联予以系统性且全方位的解析。研究成果有望为动脉粥样硬化的诊断与治疗提供新颖且极具价值的靶点与策略。This study aims to deeply analyze the role and application potential of long non-coding RNA P53 and P21 in rat atherosclerosis model. 60 rats were selected and randomly divided into normal group and atherosclerosis group. By constructing rat atherosclerosis model, a variety of experimental techniques (such as immunohistochemistry staining, real-time fluorescence quantitative PCR, protein blotting, enzyme-linked immunosorbent assay, etc.) and instruments (such as color Doppler ultrasound diagnostic instrument) were used to systematically and comprehensively analyze the expression status, regulatory mechanism and relationship between P53 and P21 and disease progression in the two groups. The research results are expected to provide novel and valuable targets and strategies for the diagnosis and treatment of atherosclerosis.展开更多
本文在耦合海气动力学基础上,研制了一个全球浅水波耦合海气模式;并模拟 EL Nino型海温异常;作了耦合系统对两种强度异常海温响应试验。通过与仅考虑大气系统动力变化的AGCM模拟试验比较,揭示了热带海洋和全球大气之间海气相互作用在气...本文在耦合海气动力学基础上,研制了一个全球浅水波耦合海气模式;并模拟 EL Nino型海温异常;作了耦合系统对两种强度异常海温响应试验。通过与仅考虑大气系统动力变化的AGCM模拟试验比较,揭示了热带海洋和全球大气之间海气相互作用在气候系统的演变中起到十分重要作用。热带海洋和全球500百帕大气平均环流在AGCM与COAGCMs中均存在明显变化。尤其是强的EL Nino信号出现时大气环流中通常为东风气流控制下的亦道地区在印度洋及其北侧出现西风,南半球的付热带西风显著加强。热带地区风场与SSTA响应明显,较高纬度也受到影响。展开更多
文摘本研究旨在深度剖析长链非编码RNA P53和P21在大鼠动脉粥样硬化模型中的作用及应用潜能。择取60只大鼠,随机平均划分为正常组与粥样硬化组,借由构建大鼠动脉粥样硬化模型,综合运用多种实验技术(如免疫组织化学染色、实时荧光定量PCR、蛋白质印迹法、酶联免疫分析等)及仪器(如彩色多普勒超声诊断仪),对两组中P53和P21的表达状况、调控机制及其与疾病演进的关联予以系统性且全方位的解析。研究成果有望为动脉粥样硬化的诊断与治疗提供新颖且极具价值的靶点与策略。This study aims to deeply analyze the role and application potential of long non-coding RNA P53 and P21 in rat atherosclerosis model. 60 rats were selected and randomly divided into normal group and atherosclerosis group. By constructing rat atherosclerosis model, a variety of experimental techniques (such as immunohistochemistry staining, real-time fluorescence quantitative PCR, protein blotting, enzyme-linked immunosorbent assay, etc.) and instruments (such as color Doppler ultrasound diagnostic instrument) were used to systematically and comprehensively analyze the expression status, regulatory mechanism and relationship between P53 and P21 and disease progression in the two groups. The research results are expected to provide novel and valuable targets and strategies for the diagnosis and treatment of atherosclerosis.
文摘本文在耦合海气动力学基础上,研制了一个全球浅水波耦合海气模式;并模拟 EL Nino型海温异常;作了耦合系统对两种强度异常海温响应试验。通过与仅考虑大气系统动力变化的AGCM模拟试验比较,揭示了热带海洋和全球大气之间海气相互作用在气候系统的演变中起到十分重要作用。热带海洋和全球500百帕大气平均环流在AGCM与COAGCMs中均存在明显变化。尤其是强的EL Nino信号出现时大气环流中通常为东风气流控制下的亦道地区在印度洋及其北侧出现西风,南半球的付热带西风显著加强。热带地区风场与SSTA响应明显,较高纬度也受到影响。
