Desire under the Elms reveals a tragedy of spiritual and survival desire of human being. O'Neill creates many Bible archetypes in Desire under the Elms. The thesis analyzes and appreciates Desire under the Elms fr...Desire under the Elms reveals a tragedy of spiritual and survival desire of human being. O'Neill creates many Bible archetypes in Desire under the Elms. The thesis analyzes and appreciates Desire under the Elms from aspect of Bible archetype to highlight the destructive power of improper desires in Desire under the Elms.展开更多
The paper explores the prospect of introducing language, perception, culture and communication. Starting with some definitions of language, perception, culture and communication, the paper argues for the internal conn...The paper explores the prospect of introducing language, perception, culture and communication. Starting with some definitions of language, perception, culture and communication, the paper argues for the internal connection among them. It provides better understanding of these factors in foreign language learning and encourages learners to achieve the better learning result to communicate effectively through language, culture etc.展开更多
从棉花cDNA文库中分离了3个编码富含甘氨酸蛋白(glycine-rich proteins,GRPs)的基因,分别命名为GhGRP1、GhGRP2、GhGRP3.推断的编码蛋白质的氨基酸序列都富含甘氨酸,甘氨酸含量超过40%.而且GhGRP1和GhGRP2蛋白质序列同源性高达99%,仅在...从棉花cDNA文库中分离了3个编码富含甘氨酸蛋白(glycine-rich proteins,GRPs)的基因,分别命名为GhGRP1、GhGRP2、GhGRP3.推断的编码蛋白质的氨基酸序列都富含甘氨酸,甘氨酸含量超过40%.而且GhGRP1和GhGRP2蛋白质序列同源性高达99%,仅在C末端有1个氨基酸残基(Arg/Pro)的差别.这3个蛋白在富含甘氨酸区相互显示较高的同源性,GhGRP1与GhGRP2达到100%,而GhGRP2与GhGRP3达到45·1%,但它们与基因数据库中其它蛋白质的同源性很低.根据结构域的组织特点,将GhGRP1和GhGRP2归为C端富含半胱氨酸结构域(C-cysteine-rich)类GRP,将GhGRP3归为N端有信号肽的GRP.GhGRP1和GhGRP2都含有12个GGX(此处X代表P/W/F)重复,GhGRP3含有22个GGX(此处X代表A/F/V/L/T/P)重复.此外,它们还含有不同数量GX,GGGX等的重复.实时RT-PCR分析表明,GhGRP1在花药中优势表达.GhGRP2在10dpa(day post anthesis)胚珠中表达最强,10dpa纤维和下胚轴次之,而在花药、根和花瓣中表达量相对较低.GhGRP3在花药,根和下胚轴中表达量较高,而在子叶,花瓣、纤维和胚珠中表达较低.上述结果表明,GhPRP基因家族的不同成员可能分别在棉花不同组织细胞的发育过程中发挥作用.展开更多
文摘Desire under the Elms reveals a tragedy of spiritual and survival desire of human being. O'Neill creates many Bible archetypes in Desire under the Elms. The thesis analyzes and appreciates Desire under the Elms from aspect of Bible archetype to highlight the destructive power of improper desires in Desire under the Elms.
文摘The paper explores the prospect of introducing language, perception, culture and communication. Starting with some definitions of language, perception, culture and communication, the paper argues for the internal connection among them. It provides better understanding of these factors in foreign language learning and encourages learners to achieve the better learning result to communicate effectively through language, culture etc.
文摘从棉花cDNA文库中分离了3个编码富含甘氨酸蛋白(glycine-rich proteins,GRPs)的基因,分别命名为GhGRP1、GhGRP2、GhGRP3.推断的编码蛋白质的氨基酸序列都富含甘氨酸,甘氨酸含量超过40%.而且GhGRP1和GhGRP2蛋白质序列同源性高达99%,仅在C末端有1个氨基酸残基(Arg/Pro)的差别.这3个蛋白在富含甘氨酸区相互显示较高的同源性,GhGRP1与GhGRP2达到100%,而GhGRP2与GhGRP3达到45·1%,但它们与基因数据库中其它蛋白质的同源性很低.根据结构域的组织特点,将GhGRP1和GhGRP2归为C端富含半胱氨酸结构域(C-cysteine-rich)类GRP,将GhGRP3归为N端有信号肽的GRP.GhGRP1和GhGRP2都含有12个GGX(此处X代表P/W/F)重复,GhGRP3含有22个GGX(此处X代表A/F/V/L/T/P)重复.此外,它们还含有不同数量GX,GGGX等的重复.实时RT-PCR分析表明,GhGRP1在花药中优势表达.GhGRP2在10dpa(day post anthesis)胚珠中表达最强,10dpa纤维和下胚轴次之,而在花药、根和花瓣中表达量相对较低.GhGRP3在花药,根和下胚轴中表达量较高,而在子叶,花瓣、纤维和胚珠中表达较低.上述结果表明,GhPRP基因家族的不同成员可能分别在棉花不同组织细胞的发育过程中发挥作用.
