五、图形E R G(Pattern ERG,PERG) 为了记录黄斑区的电活动,60年代起已开始研究由一定频率变化的光栅或黑白棋盘方格图形刺激所诱发的ERG(即PERG)并于70年代应用于临床.它是继闪光ERG之后兴起的一种检测视网膜电反应的新技术,也是现代ER...五、图形E R G(Pattern ERG,PERG) 为了记录黄斑区的电活动,60年代起已开始研究由一定频率变化的光栅或黑白棋盘方格图形刺激所诱发的ERG(即PERG)并于70年代应用于临床.它是继闪光ERG之后兴起的一种检测视网膜电反应的新技术,也是现代ERG研究的重大进展之一.PERG波形由一个小的负波和一个较大的正波组成。展开更多
Esterase D (EsD) was evaluated in 20 patients with retinoblastoma (RB). The EsD activity of the hemolysates of three unilateral cases was normal and the EsD phenotypes of their tumor cells were zero. In another three ...Esterase D (EsD) was evaluated in 20 patients with retinoblastoma (RB). The EsD activity of the hemolysates of three unilateral cases was normal and the EsD phenotypes of their tumor cells were zero. In another three cases, including one patient with bilateral RB, the EsD was heterozygous, but the tumor cells expressed enzyme from only one of the two EsD alleles. These results provided fresh evidence for Comings' theory of tumorigenesis in which the production of sporadic nonhereditary RB has been explained.展开更多
The status and expression of Rb gene was detected and analyzed in 19 surgical retinoblastoma specimens using Rb cDNA 3. 8 kb and 0. 9 kb fragment as probe and antibodies specific for synthetic Rb peptide or expressive...The status and expression of Rb gene was detected and analyzed in 19 surgical retinoblastoma specimens using Rb cDNA 3. 8 kb and 0. 9 kb fragment as probe and antibodies specific for synthetic Rb peptide or expressive product of Rb gene expression plasmld. DNA from those tumors had the hemlzygous deletion in 3 cases, the homozygous internal deletion In 2 cases and alterated restriction fragment involving In one copy of Rb gene In 1 case. The quantity of Rb protein demonstrated either absence of reduction in all the 16 cases examined In comparison with that in normal adult retina. It suggested that there were structural or/ and functional defects of Rb gene In retinoblastoma cells and provided evidence to support Knudson' s two hit hypothesis.展开更多
文摘五、图形E R G(Pattern ERG,PERG) 为了记录黄斑区的电活动,60年代起已开始研究由一定频率变化的光栅或黑白棋盘方格图形刺激所诱发的ERG(即PERG)并于70年代应用于临床.它是继闪光ERG之后兴起的一种检测视网膜电反应的新技术,也是现代ERG研究的重大进展之一.PERG波形由一个小的负波和一个较大的正波组成。
文摘Esterase D (EsD) was evaluated in 20 patients with retinoblastoma (RB). The EsD activity of the hemolysates of three unilateral cases was normal and the EsD phenotypes of their tumor cells were zero. In another three cases, including one patient with bilateral RB, the EsD was heterozygous, but the tumor cells expressed enzyme from only one of the two EsD alleles. These results provided fresh evidence for Comings' theory of tumorigenesis in which the production of sporadic nonhereditary RB has been explained.
文摘The status and expression of Rb gene was detected and analyzed in 19 surgical retinoblastoma specimens using Rb cDNA 3. 8 kb and 0. 9 kb fragment as probe and antibodies specific for synthetic Rb peptide or expressive product of Rb gene expression plasmld. DNA from those tumors had the hemlzygous deletion in 3 cases, the homozygous internal deletion In 2 cases and alterated restriction fragment involving In one copy of Rb gene In 1 case. The quantity of Rb protein demonstrated either absence of reduction in all the 16 cases examined In comparison with that in normal adult retina. It suggested that there were structural or/ and functional defects of Rb gene In retinoblastoma cells and provided evidence to support Knudson' s two hit hypothesis.
