以切花菊品种优香组培苗为试验材料,用4种磁感应强度(6、12、18、24 m T)和4种磁处理时间(2.5、5、10、20 h)的组合进行磁处理,测定磁处理后组培苗的繁殖系数、可溶性糖含量、相对电导率以及POD、SOD、CAT活性等指标。结果发现,12 m T/1...以切花菊品种优香组培苗为试验材料,用4种磁感应强度(6、12、18、24 m T)和4种磁处理时间(2.5、5、10、20 h)的组合进行磁处理,测定磁处理后组培苗的繁殖系数、可溶性糖含量、相对电导率以及POD、SOD、CAT活性等指标。结果发现,12 m T/10 h处理下组培苗繁殖系数最高,12 m T/5 h处理下组培苗可溶性糖含量最高,18 m T/20 h处理下组培苗相对电导率最低,24 m T/2.5 h处理下组培苗CAT活性最低,18 m T/10 h处理下SOD活性最低,12 m T/2.5 h处理下POD活性最低。以上结果表明,磁处理对菊花组培苗繁殖系数及生理生化具有不同程度的影响,这为今后将磁处理应用在菊花乃至其他观赏植物组织培养方面提供理论的参考。展开更多
Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hor- mone combination and explants on chrysanthemum species. The results ...Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hor- mone combination and explants on chrysanthemum species. The results indicated that stem of 9 chrysanthemum species and leaf of 6 chrysanthemum species all showed higher regeneration capacity, with regeneration rate in 84%-100% and re- generation coefficient at 2.3-9.4. However, none adventitious buds were found differ- entiated from leaves of ＂28＂ and ＂5-17＂, and leaf regeneration capacity of ＂11＂ was lower, either. Most chrysanthemum species proved higher in stem regeneration ca- pacity, instead of leaf. Therefore, chrysanthemum species can be applied for rapid reproduction and genetic engineering.展开更多
文摘以切花菊品种优香组培苗为试验材料,用4种磁感应强度(6、12、18、24 m T)和4种磁处理时间(2.5、5、10、20 h)的组合进行磁处理,测定磁处理后组培苗的繁殖系数、可溶性糖含量、相对电导率以及POD、SOD、CAT活性等指标。结果发现,12 m T/10 h处理下组培苗繁殖系数最高,12 m T/5 h处理下组培苗可溶性糖含量最高,18 m T/20 h处理下组培苗相对电导率最低,24 m T/2.5 h处理下组培苗CAT活性最低,18 m T/10 h处理下SOD活性最低,12 m T/2.5 h处理下POD活性最低。以上结果表明,磁处理对菊花组培苗繁殖系数及生理生化具有不同程度的影响,这为今后将磁处理应用在菊花乃至其他观赏植物组织培养方面提供理论的参考。
文摘Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hor- mone combination and explants on chrysanthemum species. The results indicated that stem of 9 chrysanthemum species and leaf of 6 chrysanthemum species all showed higher regeneration capacity, with regeneration rate in 84%-100% and re- generation coefficient at 2.3-9.4. However, none adventitious buds were found differ- entiated from leaves of ＂28＂ and ＂5-17＂, and leaf regeneration capacity of ＂11＂ was lower, either. Most chrysanthemum species proved higher in stem regeneration ca- pacity, instead of leaf. Therefore, chrysanthemum species can be applied for rapid reproduction and genetic engineering.
