MYB类转录因子在调控逆境应答基因的表达起着重要的作用,是最大的植物转录因子之一。文章通过同源基因克隆方法和RACE(Rapid-amplification of cDNA ends)技术,以毛竹幼苗为材料,获得一个MYB类转录因子,命名PeMYB2。氨基酸序列分析表明,...MYB类转录因子在调控逆境应答基因的表达起着重要的作用,是最大的植物转录因子之一。文章通过同源基因克隆方法和RACE(Rapid-amplification of cDNA ends)技术,以毛竹幼苗为材料,获得一个MYB类转录因子,命名PeMYB2。氨基酸序列分析表明,PeMYB2具有典型的R2R3-MYB特征,N端含有两个串联重复保守结构域,C端含有一个膜蛋白DUF3651;进化树分析表明,PeMYB2与水稻OsMYB18序列相似性最高,达到85.98%;酵母单杂实验表明,PeMYB2具有转录激活功能。将PeMYB2转化拟南芥对其功能进行分析,获得7株转基因纯合体植株。比较转基因和野生型拟南芥表型发现,PeMYB2的过量表达使转基因拟南芥出现矮化、晚花的现象;非生物胁迫处理(盐胁迫、干旱胁迫、低温胁迫)结果表明,转基因拟南芥中PeMYB2的过量表达,导致转基因植株对盐胁迫和低温胁迫有更高的耐性,但是对低温胁迫的耐受性没有明显的变化;进一步通过盐胁迫信号通路相关Marker基因(NXH1、SOS1、RD29A、COR15A)的定量PCR实验验证,发现PeMYB2对下游这些抗逆基因的表达具有调控作用。上述实验结果表明,毛竹PeMYB2可参与非生物胁迫调控,对毛竹盐胁迫和低温胁迫的响应起着重要的作用。展开更多
Objective:To explore the therapeutic effect of combination of Zhuanyindan (ZYD, 转阴丹, a self-made Chinese herbal preparation) and hormone in treating male infertility with positive antisperm antibody and its influen...Objective:To explore the therapeutic effect of combination of Zhuanyindan (ZYD, 转阴丹, a self-made Chinese herbal preparation) and hormone in treating male infertility with positive antisperm antibody and its influence on nitric oxide (NO) level. Methods:Eighty-two patients were randomly divided (according to the digital list) into the WM group (n = 20, treated with prednisone), the TCM group (n=28, treated with ZYD) and the ICWM group (n=34, treated with prednisone plus ZYD). The clinical effect, negative converting rate of antisperm antibody, changes of NO level in semen and various parameters of sperm motion before and after treatment were observed. Results: The total effective rate in the ICWM group was 88.2%, that in theTCM group 75. 0% and in the WM group 65. 0%. Significant difference was seen in the ICWM and TCM group before and after treatment in NO level, sperm motion parameters, including linear motion speed, linearity, propulsion, whip frequency, sperm vitality and mean moving angle, and quality of semen ( P < 0. 05 or P < 0. 01). In the WM group, significant difference in comparison before and after treatment was seen in NO level, propulsion, whip frequency, mean moving angle and quality of semen, including vitality and survival rate (P<0. 01). Conclusion: Combination of Chinese herbs and hormone could lower the NO level in semen and improve the quality of sperm.Original article on CJITWM (Chin) 2004 ;23 (3): 223展开更多
文摘MYB类转录因子在调控逆境应答基因的表达起着重要的作用,是最大的植物转录因子之一。文章通过同源基因克隆方法和RACE(Rapid-amplification of cDNA ends)技术,以毛竹幼苗为材料,获得一个MYB类转录因子,命名PeMYB2。氨基酸序列分析表明,PeMYB2具有典型的R2R3-MYB特征,N端含有两个串联重复保守结构域,C端含有一个膜蛋白DUF3651;进化树分析表明,PeMYB2与水稻OsMYB18序列相似性最高,达到85.98%;酵母单杂实验表明,PeMYB2具有转录激活功能。将PeMYB2转化拟南芥对其功能进行分析,获得7株转基因纯合体植株。比较转基因和野生型拟南芥表型发现,PeMYB2的过量表达使转基因拟南芥出现矮化、晚花的现象;非生物胁迫处理(盐胁迫、干旱胁迫、低温胁迫)结果表明,转基因拟南芥中PeMYB2的过量表达,导致转基因植株对盐胁迫和低温胁迫有更高的耐性,但是对低温胁迫的耐受性没有明显的变化;进一步通过盐胁迫信号通路相关Marker基因(NXH1、SOS1、RD29A、COR15A)的定量PCR实验验证,发现PeMYB2对下游这些抗逆基因的表达具有调控作用。上述实验结果表明,毛竹PeMYB2可参与非生物胁迫调控,对毛竹盐胁迫和低温胁迫的响应起着重要的作用。
文摘Objective:To explore the therapeutic effect of combination of Zhuanyindan (ZYD, 转阴丹, a self-made Chinese herbal preparation) and hormone in treating male infertility with positive antisperm antibody and its influence on nitric oxide (NO) level. Methods:Eighty-two patients were randomly divided (according to the digital list) into the WM group (n = 20, treated with prednisone), the TCM group (n=28, treated with ZYD) and the ICWM group (n=34, treated with prednisone plus ZYD). The clinical effect, negative converting rate of antisperm antibody, changes of NO level in semen and various parameters of sperm motion before and after treatment were observed. Results: The total effective rate in the ICWM group was 88.2%, that in theTCM group 75. 0% and in the WM group 65. 0%. Significant difference was seen in the ICWM and TCM group before and after treatment in NO level, sperm motion parameters, including linear motion speed, linearity, propulsion, whip frequency, sperm vitality and mean moving angle, and quality of semen ( P < 0. 05 or P < 0. 01). In the WM group, significant difference in comparison before and after treatment was seen in NO level, propulsion, whip frequency, mean moving angle and quality of semen, including vitality and survival rate (P<0. 01). Conclusion: Combination of Chinese herbs and hormone could lower the NO level in semen and improve the quality of sperm.Original article on CJITWM (Chin) 2004 ;23 (3): 223
