A double immunocytochemical staining method was used to demonstrate HBsAg and HBcAg simultaneously in the same sections of 58 hepatocellular carcinomas. Several tumor cells contained both HBsAg and HBcAg. These result...A double immunocytochemical staining method was used to demonstrate HBsAg and HBcAg simultaneously in the same sections of 58 hepatocellular carcinomas. Several tumor cells contained both HBsAg and HBcAg. These results demonstrate that hepatitis B virus (HBV) DNA in tumor cells can simultaneously express both HBsAg and HBcAg and suggest the production of a complete virus by these cells. Cytoplasmic membranous HBcAg was found not only in well differentiated hepatocellular carcinoma but also in moderate and poorly differentiated hepatocellular carcinoma. The localization of HBcAg in tumor cells appears to be the same as that of surrounding nontumorous liver tissue.展开更多
It is reported that 64DP is a tumor-related human serum DNA binding protein. The significance of its existence in serum and the mechanism of its level changing during malignant and other diseases remain unknown. 64DP ...It is reported that 64DP is a tumor-related human serum DNA binding protein. The significance of its existence in serum and the mechanism of its level changing during malignant and other diseases remain unknown. 64DP in hepatocellular carcinoma (HCC), hepatitis B, and normal livers was studied with immuno- histochemistry technique (PAP). While 12/20 HCC were positive for 64DP in the cytoplasm, the positive staining were only showed in the nuclei of most of the hepatitis B and normal livers. Auto-radiography of immobilized human chromatin with 125I-antibody against 64DP proved the existence of 64DP in chromatin. Incorporation of 3H- TdR in cultivated mouse fibroblasts showed that 64DP could inhibit cellular DNA synthesis with the inhibition rate over 30%. These results suggested that 64DP might be an inhibitory DNA binding protein, playing its physiological function in the nucleus.展开更多
文摘A double immunocytochemical staining method was used to demonstrate HBsAg and HBcAg simultaneously in the same sections of 58 hepatocellular carcinomas. Several tumor cells contained both HBsAg and HBcAg. These results demonstrate that hepatitis B virus (HBV) DNA in tumor cells can simultaneously express both HBsAg and HBcAg and suggest the production of a complete virus by these cells. Cytoplasmic membranous HBcAg was found not only in well differentiated hepatocellular carcinoma but also in moderate and poorly differentiated hepatocellular carcinoma. The localization of HBcAg in tumor cells appears to be the same as that of surrounding nontumorous liver tissue.
文摘It is reported that 64DP is a tumor-related human serum DNA binding protein. The significance of its existence in serum and the mechanism of its level changing during malignant and other diseases remain unknown. 64DP in hepatocellular carcinoma (HCC), hepatitis B, and normal livers was studied with immuno- histochemistry technique (PAP). While 12/20 HCC were positive for 64DP in the cytoplasm, the positive staining were only showed in the nuclei of most of the hepatitis B and normal livers. Auto-radiography of immobilized human chromatin with 125I-antibody against 64DP proved the existence of 64DP in chromatin. Incorporation of 3H- TdR in cultivated mouse fibroblasts showed that 64DP could inhibit cellular DNA synthesis with the inhibition rate over 30%. These results suggested that 64DP might be an inhibitory DNA binding protein, playing its physiological function in the nucleus.
