[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the ...[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the research materials,total DNA was extracted from transgenic Brassica napus by using modified CTAB method.After enzyme digestion and purification,self-joining was made.Two circles of nested PCR and the sequence alignment were carried out.[Result] A fragement with the size of 4.0 kb was amplified ...展开更多
通过不同甘蓝型油菜中SLG基因的克隆及序列分析,探索了甘蓝型油菜中是否存在与芸薹属二倍体中相同或相似的S-locus基因。对10个甘蓝型油菜品种和品系中SLG基因的PCR扩增和序列比较分析发现,这些甘蓝型油菜中都存在第2类的SLG基因,而且,...通过不同甘蓝型油菜中SLG基因的克隆及序列分析,探索了甘蓝型油菜中是否存在与芸薹属二倍体中相同或相似的S-locus基因。对10个甘蓝型油菜品种和品系中SLG基因的PCR扩增和序列比较分析发现,这些甘蓝型油菜中都存在第2类的SLG基因,而且,同二倍体芸薹属物种一样,第2类SLG基因之间具有较高的同源性;只有5个甘蓝型油菜品种和品系中存在第一类SLG基因,而且这些基因序列之间表现出高度的保守性,即同源性在96%以上,明显高于不同等位基因之间的同源性。这些甘蓝型油菜中的class I SLG基因可能源于同一个自交不亲和单体。展开更多
基金Supported by National 863 Program of China(2006AA10A113)Natural Science foundation of Zhejiang Province(Y306097)~~
文摘[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the research materials,total DNA was extracted from transgenic Brassica napus by using modified CTAB method.After enzyme digestion and purification,self-joining was made.Two circles of nested PCR and the sequence alignment were carried out.[Result] A fragement with the size of 4.0 kb was amplified ...
文摘通过不同甘蓝型油菜中SLG基因的克隆及序列分析,探索了甘蓝型油菜中是否存在与芸薹属二倍体中相同或相似的S-locus基因。对10个甘蓝型油菜品种和品系中SLG基因的PCR扩增和序列比较分析发现,这些甘蓝型油菜中都存在第2类的SLG基因,而且,同二倍体芸薹属物种一样,第2类SLG基因之间具有较高的同源性;只有5个甘蓝型油菜品种和品系中存在第一类SLG基因,而且这些基因序列之间表现出高度的保守性,即同源性在96%以上,明显高于不同等位基因之间的同源性。这些甘蓝型油菜中的class I SLG基因可能源于同一个自交不亲和单体。
