Aim To investigate the protective effect of hydroxysafflor yellow A（HSYA）,a soluble element extracted from Carthamus tinctorius L.,on focal cerebral ischemia in rats.Methods Focal cerebral ischemia in male Wistar-Ky...Aim To investigate the protective effect of hydroxysafflor yellow A（HSYA）,a soluble element extracted from Carthamus tinctorius L.,on focal cerebral ischemia in rats.Methods Focal cerebral ischemia in male Wistar-Kyoto（WKY） rats were induced by permanent middle cerebral artery occlusion（MCAO）.Three doses of 1.5,3.0 and 6.0 mg·kg-1 of HSYA were administrated to three groups of rats,separately,via sublingular vein injection 30 min after the onset of ischemia.24 h after ischemia in rats,neurological deficit scores were evaluated and the infarction area of brain was assessed by quantitative image analysis.The in vitro neuroprotective effect of HSYA was tested in cultured fetal cortical neurons exposed to glutamate and sodium cyanide（NaCN）.Results HSYA at doses of 3.0 and 6.0(mg·kg-1) exerted significant neuroprotective effects on rats with focal cerebral ischemic injury as expressed by neurological deficit scores and reduced the infarct area as compared with saline group,and the potency of HSYA at dose of 6.0 mg·kg-1 was similar to that of 0.2 mg·kg-1 of nimodipine.In vitro studies,HSYA significantly inhibited neurons damage induced by exposure to glutamate and NaCN in cultured fetal cortical cells.Conclusion HSYA has potential neuroprotective action against focal cerebral ischemia in rats and cultured rat fetal cortical neurons as well.展开更多
文摘Aim To investigate the protective effect of hydroxysafflor yellow A（HSYA）,a soluble element extracted from Carthamus tinctorius L.,on focal cerebral ischemia in rats.Methods Focal cerebral ischemia in male Wistar-Kyoto（WKY） rats were induced by permanent middle cerebral artery occlusion（MCAO）.Three doses of 1.5,3.0 and 6.0 mg·kg-1 of HSYA were administrated to three groups of rats,separately,via sublingular vein injection 30 min after the onset of ischemia.24 h after ischemia in rats,neurological deficit scores were evaluated and the infarction area of brain was assessed by quantitative image analysis.The in vitro neuroprotective effect of HSYA was tested in cultured fetal cortical neurons exposed to glutamate and sodium cyanide（NaCN）.Results HSYA at doses of 3.0 and 6.0(mg·kg-1) exerted significant neuroprotective effects on rats with focal cerebral ischemic injury as expressed by neurological deficit scores and reduced the infarct area as compared with saline group,and the potency of HSYA at dose of 6.0 mg·kg-1 was similar to that of 0.2 mg·kg-1 of nimodipine.In vitro studies,HSYA significantly inhibited neurons damage induced by exposure to glutamate and NaCN in cultured fetal cortical cells.Conclusion HSYA has potential neuroprotective action against focal cerebral ischemia in rats and cultured rat fetal cortical neurons as well.
文摘目的研究人参皂苷CK对CC l4致慢性肝损伤的影响。方法用CC l4致大鼠慢性肝损伤模型,观察人参皂苷CK(0.3,1,3 mg/kg)对大鼠血清天冬氨酸转氨酶(aspartate transam inase,ALT)、丙氨酸转氨酶(alan ine transam inase,AST)、超氧化物歧化酶(superoxide d ismutase,SOD)、丙二醛(m alond ialdehyde,MDA)及透明质酸(hyaluron ic ac id,HA)、Ⅲ型前胶原(pre-collagenⅢ,PCⅢ)的影响,并对肝脏组织进行病理学观察。结果人参皂苷CK小剂量(0.3 mg/kg)能降低血清转氨酶ALT,AST水平,增加血清SOD的含量,降低MDA含量;CK中、高剂量无明显作用。CK各剂量组血清HA、PCⅢ和肝组织病理未见明显改变。结论CK低剂量对CC l4致慢性肝损伤具有一定的保护作用,其作用可能与抗氧化有关。
