摘要
从热水浸提过的阿魏侧耳 (Pleurotusferulae)子实体中用 3%的三氯乙酸提取出另一种水溶性粗多糖PFA ,将PFA经Sevage法脱蛋白、透析、CTAB络合去沉淀后得多糖PF3。苯酚硫酸法测得PF3糖含量为 94 1 % ;用光散射和GPC联机测得PF3重均分子量为 1 96 5× 1 0 6 ;用IR和GC分析了组成和结构 ,PF3由鼠李糖、木糖、葡萄糖、半乳糖和甘露糖组成 ,含有α 糖苷键。同时研究了PF3对核糖核酸酶的抑制作用 ,表明在浓度 5mg mL时其抑制率可达4 4 4 %。
The water soluble polysaccharide PFA isolated by 3% trichloroacetic acid extraction from residues of fruit bodies of Pleurotus ferulae , Proteins were removed from PFA by Sevag method, and dialysed with water, precipitated with CTAB, and supernatant precipitated with alcohol, PF 3 was obtained. PF 3 contained 94 1% carbohydrate, the Mw of PF 3 was 1 975×10 6 The components and structure of PF 3 were analyzed by IR and GC. PF 2 consisted of rhamnose, xylose, glucose, galactose, mannose; and PF 3 contained α glucoside. It was found PF 3 had inhibition on RNase,inhibition rate was 44 4% at 5mg/mL concentration of PF 3.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第1期101-103,共3页
Acta Microbiologica Sinica
基金
湖北省重点科技计划资助项目 ( 95 2P10 0 7)~~
