摘要
【目的】在已构建GOC(乙醇酸氧化酶OsGLO、草酸氧化酶OsOXO和过氧化物酶OsCAT)光呼吸支路的工程水稻中,敲除乙醇酸转运体编码基因OsPLGG1a或OsPLGG1b,以优化光呼吸支路的改造,提高水稻光合效率。【方法】以GOC工程水稻为背景材料,通过CRISPR-Cas9技术分别敲除OsPLGG1a或OsPLGG1b基因,通过抗性筛选和测序鉴定osplgg1a-GOC和osplgg1b-GOC纯合敲除株系,并测定光合速率。【结果】获得敲除OsPLGG1a或OsPLGG1b的GOC代谢支路的纯合株系(osplgg1a-GOC和osplgg1b-GOC)。osplgg1a-GOC植株与ZH11背景中OsPLGG1a敲除突变体表型相似,均表现出黄化矮小生长抑制的表型;osplgg1b-GOC植株相比GOC水稻的净光合速率提高,说明OsPLGG1b突变有利于叶绿体中滞留乙醇酸,增加GOC支路的代谢通量,进一步提高叶绿体中的CO_(2)浓度。【结论】相对于GOC水稻,osplgg1b-GOC部分株系的净光合速率和气孔导度增加,暗示敲除OsPLGG1b可用于光呼吸代谢支路的优化。
【Objective】By deleting either OsPLGG1a or OsPLGG1b to optimize the pathway in the pre-engineered GOC rice for enhancing photosynthesis and photorespiration of the plant was postulated.【Method】The knockout vectors osplgg1a-Cas9 or osplgg1b-Cas9 were constructed and transformed into the GOC rice to obtain transgenic homozygous lines with OsPLGG1a or OsPLGG1b deleted.Resulting photosynthesis and photorespiration of the rice plant were measured to determine the validity of the proposed hypothesis.【Result】The transgenic homozygous lines were successfully obtained to show a similar phenotype of osplgg1a-GOC and osplgg1a plants with stunted growth.The osplgg1b-GOC plants displayed a net photosynthetic rate increase over that of GOC rice.That indicated the OsPLGG1b mutation to be conducive to the retention of chloroplast glycolate,rise of metabolic flux through GOC bypass,and reduction of plant photorespiration metabolism manifested with enriched CO_(2) in the chloroplasts.【Conclusion】Compared with the GOC rice,the transgenic osplgg1b-GOC plants had an elevated net photosynthetic rate and stomatal conductance.It suggested that the deletion of OsPLGG1b could be a means to enhance the photorespiration metabolism in a rice plant.
作者
崔丽丽
蔡秋华
邱洁瑜
高蓉蓉
赵永超
王颖姮
彭新湘
朱国辉
张建福
CUI Lili;CAI Qiuhua;QIU Jieyu;GAO Rongrong;ZHAO Yongchao;WANG Yingheng;PENG Xinxiang;ZHU Guohui;ZHANG Jianfu(Rice Research Institute,Fujian Academy of Agricultural Sciences/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China,Ministry of Agriculture and Rural Affairs/Fuzhou Branch,National Rice Improvement Center of China/National Engineering Laboratory of Rice/Incubator of Key Laboratory of Crop Germplasm Enhancement and Molecular Breeding between Fujian and Ministry of Sciences and Technology/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Research Base of South-China of National Key Laboratory of Hybrid Rice of China,Fuzhou,Fujian 350003,China;College of Life Sciences,South China Agricultural University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangzhou,Guangdong 510642,China)
出处
《福建农业学报》
2024年第10期1111-1119,共9页
Fujian Journal of Agricultural Sciences
基金
国家自然科学基金项目(32201733)
福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021019-SDS01)
中央引导地方科技发展专项(2022L3018)
福建省自然科学基金项目(2022J01453)
福建省科技计划公益类专项(2023R1068、2020R1023008)
福建省农业科学院科研项目(ZYTS202203、GJYS202301)
