摘要
转录因子XBP1是真菌发育和其他生物过程的关键调节因子。利用RNAi法探究其在糙皮侧耳生长发育过程中的功能。以糙皮侧耳CCMSSC00389为材料,克隆获得PoXBP1基因,全长1326 bp,编码441个氨基酸,生物信息学分析结果表明XBP1蛋白含有HTH APSES结构域,无信号肽,是非跨膜结构蛋白。在野生型菌株的不同发育阶段的表达显示,PoXBP1基因在菌丝、原基、孢子、菌盖、菌柄中的表达依次降低。通过农杆菌介导的遗传转化法筛选获得PoXBP1干扰菌株,其中,干扰菌株RNAiPoXBP1-1和RNAiPoXBP1-4的生长速度慢于野生型菌株,表明了PoXBP1表达量降低,延缓了糙皮侧耳菌丝和子实体的生长速度。
Transcription factor XBP1 is a key regulatory factor for fungi development and other biological processes.In this study,RNA interference method was used to explore its function in the growth and development of Pleurotus ostreatus.The PoXBP1 gene was cloned from the CCMSSC00389(Pleurotus ostreatus).The full-length of PoXBP1 was 1326 bp and it encoded a protein of 441 amino acids.Bioinformatics analysis showed that XBP1 protein contained HTH APSES domain and had no signal peptide.It was a non transmembrane structural protein.The expression of PoXBP1 gene in different developmental stages of wild type strains decreased in turn in hyphae,primordium,spores,cap and stalk.PoXBP1 interfering strain were screened by Agrobacterium tumefaciens-mediated genetic transformation.Among them,the growth rates of interfering strain RNAiPoXBP1-1 and RNAiPoXBP1-4 were slower than that of wild-type strains,indicating that the expression of PoXBP1 decreased and the growth rate of hyphae and fruiting bodies of P.ostreatus delayed.
作者
齐雨慧
黄晨阳
张利姣
QI Yu-hui;HUANG Chen-yang;ZHANG Li-jiao(Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Key Laboratory of Microbial Resources,Ministry of Agriculture and Rural Affairs,Beijing 100081)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第11期65-71,共7页
Biotechnology Bulletin
基金
中央级公益性科研院所基本科研业务费专项(1610132020004)。
关键词
糙皮侧耳
转录因子
XBP1
生长发育
Pleurotus ostreatus
transcription factor
XBP1
growth and development
