摘要
目的探讨可溶性共刺激分子B7-H3(sB7-H3)和干扰素γ(IFN-γ)在肺炎支原体肺炎(MPP)患儿肺泡灌洗液(BALF)中浓度变化及作用机制,为MPP的临床诊断、评估及治疗提供依据。方法选择重症MPP患儿35例(重症组)及轻症MPP(轻症组)患儿35例为实验组,以同期行纤维支气管镜异物取出术的患儿15例作为对照组。采用酶联免疫吸附试验(ELISA法)检测BALF中sB7-H3、IFN-γ表达水平,并比较各组间的差异,以及MPP患儿急性期BALF中sB7-H3水平与IFN-γ水平的相关性。结果重症组BALF中sB7-H3、IFN-γ水平显著高于轻症组及对照组,差异有统计学意义(P<0.05)。轻症组BALF中sB7-H3、IFN-γ水平显著高于对照组,差异有统计学意义(P<0.05或P<0.01)。MPP组急性期BALF中sB7-H3与IFN-γ的相关系数r为0.941(P<0.05),表明在MPP中IFN-γ的表达水平与sB7-H3表达水平呈正相关。结论在儿童MPP中sB7-H3可能通过调控IFN-γ等炎症因子的分泌,参与重症MPP的发生发展。
Objective To explore the changes of sB7-H3 and IFN-γ in the bronchoalveolar lavage fluid(BACF)of children with Mycoplasma pneumoniae pneumonia(MPP),so as to provide evidence for clinical diagnosis,evaluation and treatment of MPP.Methods A total of 35 children with severe MPP and 35 children with slight MPP were selected as an experiment group,while 15 children who underwent fiber broncoscopy were selected as acontrol group.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of sB7-H3 and IFN-γ in BALF,and to compare the differences between the groups,as well as the correlation between sB7-H3 and IFN-γlevel in acute phase BALF of MPP children.Results The severe MPP group produced remarkably higher levels of sB7-H3 and IFN-γ in BALF than those in the slight MPP group and the control group(P<0.05).The slight MPP group produced remarkably higher levels of sB7-H3 and IFN-γthan the control group(P<0.05 or P<0.01).The correlation coefficient r of sB7-H3 and IFN-γin acute BALF of the MPP group was 0.941(P<0.05),indicating that the level of IFN-γ in MPP was positively correlated with the expression of sB7-H3.Conclusions sB7-H3 may be involved in the pathogenesis of severe MPP by regulating the secretion of inflammatory factors such as IFN-γ.
作者
姜之焕
武怡
JIANG Zhihuan;WU Yi(Department of Pediatrics,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu 221002,China)
出处
《徐州医科大学学报》
CAS
2020年第2期114-117,共4页
Journal of Xuzhou Medical University
