摘要
研究了干露胁迫对日本囊对虾(Marsupenaeus japonicus)抗氧化酶活性的影响。结果显示,与对照组相比,4个实验组的对虾均于干露胁迫5 h后出现大量死亡,而低温湿润组对虾成活率高于其他实验组;随干露胁迫时间的增加,4个实验组对虾肝胰腺中谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性呈现先升高后降低的趋势,但低温干燥(DG)和常温湿润(CS)组GPx活性在胁迫早期有显著降低现象(P<0.05);谷胱甘肽-S转移酶(GST)和过氧化物酶(POD)活性显著降低(P<0.05);丙二醛(MDA)含量呈现先降低后升高的趋势。结果表明,干露胁迫显著诱导日本囊对虾氧化应激反应,GPx、CAT、SOD和MDA可作为其应答干露胁迫反应的监测指标。适量的低温湿润条件可提高对虾干法运输的成活率,该条件下体质量约10 g的日本囊对虾在干露胁迫10 h内具有较高的成活率。
We investgated the effect of desiccation on antioxidant enzyme activities of kuruma shrimp （Marsupenaeus japonicus）. The results show that many shrimps died in the four treatment groups in 5 h after desiccation, and wet and low temperature improved the survival rate of shrimps significantly. Compared with the control, the activities of glutathione peroxidase （GPx）, catalase （CAT） and superoxide dismutase （SOD） in hepatopancreas of the four treatment groups increased firstly and then decreased gradually, but GPx activities in the low temperature and dry （DG） and the normal temperature and wet （CS） groups decreased significantly at the begin- ning of the experiment （ P 〈 0. 05 ）. The activities of glutathione S-transferase （GST） and peroxidase （ POD ） decreased significantly （P 〈0.05）. The contents of malondialdehyde （MI）A） decreased firstly and then increased gradually. These results indicate that the response to oxidative stress of M. japonicus could be induced by desiccation. GPx, CAT, SOD and MDA were all sensitive to desicca- tion and could be regarded as monitor indices for desiccation in M. japonicus. It is beneficial to keep appropriate low and wet tempera- ture during the transportation of M.japonicus without water, and the shrimps of average body weight of about 10 g would have a higher survival rate under such condition after 10-hour desiccation.
出处
《南方水产科学》
CAS
CSCD
北大核心
2015年第4期102-108,共7页
South China Fisheries Science
基金
国家科技支撑计划项目(2011BAD13B10)
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2014TS15
2012YD02)
广东省海洋渔业科技推广专项(A201101E02
A201208H01
A201201B04)
关键词
日本囊对虾
干露
抗氧化酶
成活
Marsupenaeus japonicus
desiccation
antioxidant enzyme
survival
