摘要
目的:研究莪术抗癌成分β-榄香烯诱导肝癌细胞早期凋亡的作用及机制。方法:采用 Annex-in-V-FITC 标记后流式细胞术分析和 Fluo-3负载后激光扫描共聚焦显微镜实时监测,观察β-榄香烯诱导小鼠肝癌细胞早期凋亡效应及细胞内游离钙离子的变化。结果:(1)50 μg/ml 浓度的β-榄香烯可诱导肝癌细胞早期凋亡(P<0.01),且呈剂量依赖性;(2)β-榄香烯浓度为50 μg/ml 时,早期凋亡细胞内游离钙离子水平先一过性升高后迅速下降。结论:β-榄香烯可诱导肝癌细胞早期凋亡,其机制与细胞内游离 Ca^(2+)的变化有关。
Objective:To study the effect and mechanism of β-elemene,an active principle of Curcuma aromatica,in inducing liver carcinoma cell apoptosis.Methods:The effect of β-elemene in inducing apoptosis of liver carcinoma cell in early stage and on change of intracellular calcium ion in mice were monitored in real-time by flow cytometry after Annexin-V-FITC labeling and laser scanning confocal mi- croscopy with fluo-3/AM loading.Results:β-elemene in 50μg/ml concentration could induce the early stage apoptosis of li...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2002年第S1期190-192,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助课题(No.39730440-Ⅱ)
